RLG00000026162

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004049
Physical Location & Seq
Forward (+)
903168 .. 903653
486 bp
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UTR
Exon/CDS
Intron
RLM00000026162

Sequence Viewer

Length: 486 bp
ATGGGCAACTGTTTTGTTCTTTGCAGAATCAGTACTGCTTCTCGGATTGTTTCTGAGGATGTTGCACCGAAGCAAGGAAAAGTTGTGAAGGTTCTGAAGATGGACGGGAAAGTTTTAGAATTCAGGTCACCTATTCTTGTCAAGAATGTCTTGTTGAGCTTTTCAGACTCTGCAAGTATAGGTTTATCAAAGCAATTAGCTTCAGAACATCTCCCTGCAAATTATGAACTGAAGTTAGGGAAGTTTTACTGTATGGTTCCTCTTCTTGGTTCTGTTACAAAAAACAACAGGACAGCTTCTGCTACAACAAGAAGGATTAAAATTGTAATAACTAGGCAACAGCTTCAGGAGCTTTTGACAAACCAAGTATCAGTCCAGAAGTTACTTTCATTTTCAGGGCCGAAAATCAAACCGTGTAATTTGGTTGATCCTTCCACAATTTGGAAGCCTAAGCTTCAGGTCATCCCCGAAGGAAGCGAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

162

Amino Acids

17.78

Weight (kDa)

9.85

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 157 2.1e-14 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 441
AclWI GGATC 1 cut(s) 422
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 5 cut(s) 116, 186, 251, 329, 440
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 3 cut(s) 74, 266, 441
AluBI AGCT 6 cut(s) 159, 200, 296, 343, 352, 454
AluI AGCT 6 cut(s) 159, 200, 296, 343, 352, 454
Alw21I GWGCWC 1 cut(s) 483
AlwI GGATC 1 cut(s) 422
AlwNI CAGNNNCTG 2 cut(s) 170, 299
AoxI GGCC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 483
BccI CCATC 1 cut(s) 94
BfaI CTAG 1 cut(s) 333
BmcAI AGTACT 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 450
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 266, 441
BseGI GGATG 2 cut(s) 64, 462
BseLI CCNNNNNNNGG 3 cut(s) 74, 266, 441
BseMII CTCAG 1 cut(s) 45
BshFI GGCC 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 483
BslI CCNNNNNNNGG 3 cut(s) 74, 266, 441
BsnI GGCC 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 1 cut(s) 427
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 1 cut(s) 422
BssMI GATC 1 cut(s) 427
Bst4CI ACNGT 3 cut(s) 11, 251, 414
Bst6I CTCTTC 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 2 cut(s) 54, 450
BstEII GGTNACC 1 cut(s) 126
BstF5I GGATG 2 cut(s) 64, 462
BstKTI GATC 1 cut(s) 430
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstPI GGTNACC 1 cut(s) 126
BsuRI GGCC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 64, 462
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 479
CaiI CAGNNNCTG 2 cut(s) 170, 299
Cfr13I GGNCC 1 cut(s) 398
Csp6I GTAC 1 cut(s) 33
CviJI RGCY 8 cut(s) 159, 200, 296, 343, 352, 400, 448, 454
CviKI_1 RGCY 8 cut(s) 159, 200, 296, 343, 352, 400, 448, 454
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 2 cut(s) 54, 450
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco57I CTGAAG 5 cut(s) 116, 186, 251, 329, 440
Eco91I GGTNACC 1 cut(s) 126
EcoO65I GGTNACC 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 119
FaiI YATR 3 cut(s) 179, 225, 254
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FokI GGATG 2 cut(s) 71, 449
FspBI CTAG 1 cut(s) 333
HaeIII GGCC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 2 cut(s) 27, 167
HphI GGTGA 1 cut(s) 120
Hpy188I TCNGA 5 cut(s) 45, 55, 96, 166, 205
Hpy188III TCNNGA 3 cut(s) 142, 347, 376
HpyAV CCTTC 4 cut(s) 82, 306, 441, 464
HpyCH4III ACNGT 3 cut(s) 11, 251, 414
HpyCH4V TGCA 4 cut(s) 24, 65, 173, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 2 cut(s) 54, 450
Kzo9I GATC 1 cut(s) 427
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 7 cut(s) 109, 228, 274, 332, 381, 389, 443
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 126, 274, 381
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 2 cut(s) 109, 254
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 6 cut(s) 119, 194, 220, 321, 418, 438
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 2 cut(s) 49, 270
MseI TTAA 1 cut(s) 318
MwoI GCNNNNNNNGC 1 cut(s) 349
NdeII GATC 1 cut(s) 427
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 441
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PspEI GGTNACC 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 398
PstNI CAGNNNCTG 2 cut(s) 170, 299
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 1 cut(s) 427
Sau96I GGNCC 1 cut(s) 398
ScaI AGTACT 1 cut(s) 34
SchI GAGTC 1 cut(s) 161
SduI GDGCHC 1 cut(s) 483
Sse9I AATT 6 cut(s) 119, 194, 220, 321, 418, 438
SspMI CTAG 1 cut(s) 333
TaaI ACNGT 3 cut(s) 11, 251, 414
TasI AATT 6 cut(s) 119, 194, 220, 321, 418, 438
TatI WGTACW 1 cut(s) 32
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseFI GTSAC 1 cut(s) 126
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 2 cut(s) 240, 378
Van91I CCANNNNNTGG 1 cut(s) 441
XapI RAATTY 1 cut(s) 119
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.