RchiOBHm_Chr3g0473821

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004049
Physical Location & Seq
Reverse (-)
19524890 .. 19525686
797 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43948

Sequence Viewer

Length: 606 bp
ATGAAGTATGAGGGGAAGGTTGTAACCATCTACTTTAAATACCACTCTTATTCCCACCACCCTCATTCCTTCACAACTTTTAAAACTAAAGTTTTGTCACCAGGCTTTGATAAAACAGGAGCAATGGGAAATTGCTTGGTTTTGCAACAAAATGTTACAAAGATAATGAAACCAGATGGGAAAGTCCTAGAGTACAGAGCATCCATAAAAGTTTACCAGGTGTTGTCCGAGTTTTCTGGCCACGCAATATCTGAGGCGACTCCGGTGTACCAGCATCTCCGGCCAGATACAAAGCTGCTCGGTGGTCGCCTGTACTATCTTGTACCTCTGCCTTTGCCGTCAGAGAAGGCATCACAGAAGAAGGTGAGGTTTTCAACTGATCCTGAAGGTGAAGCTCAACAAGAAACTAAAGTTGTAAGAATTAAGCTAGTTATAAGCAAGAAACAACTGCAGGAGATGTTGGGAAAGGGAGGGGTTTCAGTTGATGACATGGTTTCCAGAATTCAATGTCAAACAAGCACAGATAAGAAGACTAACAGCTCCGACAACGATGGTAGTTGTGAAGGGTGGAAACCAGTTTTAGAAAGCATACCTGAAGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

201

Amino Acids

22.53

Weight (kDa)

9.19

Isoelectric Point (pI)

24.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 42 - 199 5.2e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 2 cut(s) 238, 281
AcsI RAATTY 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 4 cut(s) 194, 269, 314, 324
AgsI TTSAA 2 cut(s) 375, 506
AjnI CCWGG 2 cut(s) 100, 216
AluBI AGCT 4 cut(s) 295, 395, 427, 540
AluI AGCT 4 cut(s) 295, 395, 427, 540
AlwI GGATC 1 cut(s) 374
AoxI GGCC 2 cut(s) 238, 281
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 1 cut(s) 501
AsuHPI GGTGA 3 cut(s) 90, 376, 401
BalI TGGCCA 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 536
BbvI GCAGC 1 cut(s) 282
BccI CCATC 3 cut(s) 35, 170, 545
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 2 cut(s) 102, 218
BfaI CTAG 3 cut(s) 188, 428, 604
BfmI CTRYAG 1 cut(s) 449
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 2 cut(s) 102, 218
BmrFI CCNGG 2 cut(s) 102, 218
BmsI GCATC 3 cut(s) 209, 283, 359
BpiI GAAGAC 1 cut(s) 536
BsaWI WCCGGW 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 575
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 2 cut(s) 102, 218
BseGI GGATG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 282
BshFI GGCC 2 cut(s) 240, 283
BsiSI CCGG 2 cut(s) 263, 280
BsnI GGCC 2 cut(s) 240, 283
Bsp143I GATC 1 cut(s) 379
BspANI GGCC 2 cut(s) 240, 283
BspCNI CTCAG 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 453
BspPI GGATC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 129
BsrI ACTGG 1 cut(s) 575
BssMI GATC 1 cut(s) 379
Bst2UI CCWGG 2 cut(s) 102, 218
BstDEI CTNAG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 200
BstKTI GATC 1 cut(s) 382
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 2 cut(s) 102, 218
BstSCI CCNGG 2 cut(s) 100, 216
BstSFI CTRYAG 1 cut(s) 449
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 240, 283
BtsCI GGATG 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 216
Csp6I GTAC 4 cut(s) 193, 268, 313, 323
CviAII CATG 1 cut(s) 490
CviJI RGCY 7 cut(s) 105, 240, 283, 295, 395, 427, 540
CviKI_1 RGCY 7 cut(s) 105, 240, 283, 295, 395, 427, 540
CviQI GTAC 4 cut(s) 193, 268, 313, 323
DdeI CTNAG 1 cut(s) 252
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
DraI TTTAAA 2 cut(s) 37, 82
EaeI YGGCCR 2 cut(s) 238, 281
Eco57I CTGAAG 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 501
EcoRII CCWGG 2 cut(s) 100, 216
FaeI CATG 1 cut(s) 493
FaiI YATR 5 cut(s) 9, 206, 434, 491, 590
FatI CATG 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 3 cut(s) 188, 428, 604
GluI GCNGC 1 cut(s) 296
HaeIII GGCC 2 cut(s) 240, 283
HapII CCGG 2 cut(s) 263, 280
Hin1II CATG 1 cut(s) 493
HinfI GANTC 1 cut(s) 259
HpaII CCGG 2 cut(s) 263, 280
HphI GGTGA 3 cut(s) 90, 376, 401
Hpy166II GTNNAC 3 cut(s) 214, 268, 601
Hpy188I TCNGA 4 cut(s) 229, 253, 343, 544
Hpy188III TCNNGA 2 cut(s) 383, 498
Hpy8I GTNNAC 3 cut(s) 214, 268, 601
HpyAV CCTTC 6 cut(s) 10, 79, 340, 355, 380, 557
HpyCH4V TGCA 2 cut(s) 145, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 1 cut(s) 252
Hsp92II CATG 1 cut(s) 493
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 2 cut(s) 119, 545
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 3 cut(s) 209, 283, 359
MabI ACCWGGT 1 cut(s) 216
MaeI CTAG 3 cut(s) 188, 428, 604
MaeIII GTNAC 3 cut(s) 22, 96, 154
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 2 cut(s) 370, 541
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 3 cut(s) 130, 420, 501
MluNI TGGCCA 1 cut(s) 240
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 567
MnlI CCTC 6 cut(s) 4, 72, 247, 336, 360, 464
Mox20I TGGCCA 1 cut(s) 240
MscI TGGCCA 1 cut(s) 240
MseI TTAA 3 cut(s) 36, 81, 423
Msp20I TGGCCA 1 cut(s) 240
MspI CCGG 2 cut(s) 263, 280
MspR9I CCNGG 2 cut(s) 102, 218
MvaI CCWGG 2 cut(s) 102, 218
MwoI GCNNNNNNNGC 1 cut(s) 280
NdeII GATC 1 cut(s) 379
NlaIII CATG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 96
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PsiI TTATAA 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 100, 216
PspGI CCWGG 2 cut(s) 100, 216
PstI CTGCAG 1 cut(s) 453
RsaI GTAC 4 cut(s) 194, 269, 314, 324
RsaNI GTAC 4 cut(s) 193, 268, 313, 323
SaqAI TTAA 3 cut(s) 36, 81, 423
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 1 cut(s) 379
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 102, 218
SexAI ACCWGGT 1 cut(s) 216
SfaNI GCATC 3 cut(s) 209, 283, 359
SfcI CTRYAG 1 cut(s) 449
Sse9I AATT 3 cut(s) 130, 420, 501
SspMI CTAG 3 cut(s) 188, 428, 604
StyD4I CCNGG 2 cut(s) 100, 216
TasI AATT 3 cut(s) 130, 420, 501
TatI WGTACW 2 cut(s) 192, 312
Tru1I TTAA 3 cut(s) 36, 81, 423
Tru9I TTAA 3 cut(s) 36, 81, 423
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 2 cut(s) 17, 182
XapI RAATTY 1 cut(s) 501
XspI CTAG 3 cut(s) 188, 428, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.