Rh1CG431300

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004049
Physical Location & Seq
Reverse (-)
68830759 .. 68831253
495 bp
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UTR
Exon/CDS
Intron
Rh1CG431300.1

Sequence Viewer

Length: 435 bp
ATGGGCAACTGTTTTGTTCTTTGCAGAATCAGTACTGCTTCTCGGATTGTTTCTGAGGATGTTGCTTCGAAGCAAGGAAAAGTTGTGAAGGTTCTGAAGATGGACGGGAAAGTTTTAGAATTCAGGGCACCTATTCTTGTCAAGAATGTCTTGTTGAGCTTTTCAGGCTCTGCAGGTATAGGTTTAGGGAAGTTTTACTATATGGTTCCTCTTCTTCGTTCTGTTACAAAAAACAACAGGACAGCTTCTGCTACAACAAGAAGGATTAAAATTGTCATAACTAGGCAACAGCTTCAGGAGCTTTTGACAAACCAAGTATCAGTCCAGAAGTTACTTTCATTTTCAGGGCCGAAAATTAAACCGTGTAATTTGGTTGATCCTTCCACAAATTGGAAGCCTAAGCTTCAGGTCATCCCTGAAGGAAGCGAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

144

Amino Acids

15.9

Weight (kDa)

10.23

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 56 1.8e-06 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 390
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 3 cut(s) 116, 278, 389
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 1 cut(s) 390
AluBI AGCT 5 cut(s) 159, 245, 292, 301, 403
AluI AGCT 5 cut(s) 159, 245, 292, 301, 403
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 2 cut(s) 170, 248
AoxI GGCC 1 cut(s) 347
ApoI RAATTY 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 347
AsuII TTCGAA 1 cut(s) 68
BaeGI GKGCMC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 1 cut(s) 94
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 164
BmcAI AGTACT 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 129, 207
Bpu10I CCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 390
BseGI GGATG 2 cut(s) 64, 411
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 130
BshFI GGCC 1 cut(s) 349
BshNI GGYRCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 390
BsnI GGCC 1 cut(s) 349
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 2 cut(s) 130, 432
Bsp143I GATC 1 cut(s) 376
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 129, 207
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 1 cut(s) 371
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 127
BssMI GATC 1 cut(s) 376
Bst4CI ACNGT 2 cut(s) 11, 363
Bst6I CTCTTC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 428
BstDEI CTNAG 2 cut(s) 54, 399
BstF5I GGATG 2 cut(s) 64, 411
BstKTI GATC 1 cut(s) 379
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 2 cut(s) 165, 298
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 130
BsuRI GGCC 1 cut(s) 349
BtsCI GGATG 2 cut(s) 64, 411
BtsIMutI CAGTG 1 cut(s) 430
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 428
CaiI CAGNNNCTG 2 cut(s) 170, 248
Cfr13I GGNCC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 33
CviJI RGCY 8 cut(s) 159, 168, 245, 292, 301, 349, 397, 403
CviKI_1 RGCY 8 cut(s) 159, 168, 245, 292, 301, 349, 397, 403
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 2 cut(s) 54, 399
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco57I CTGAAG 3 cut(s) 116, 278, 389
EcoRI GAATTC 1 cut(s) 119
FaiI YATR 4 cut(s) 179, 201, 203, 278
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FokI GGATG 2 cut(s) 71, 398
FspBI CTAG 1 cut(s) 282
HaeIII GGCC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 1 cut(s) 27
Hpy188I TCNGA 3 cut(s) 45, 55, 96
Hpy188III TCNNGA 3 cut(s) 142, 296, 325
HpyAV CCTTC 4 cut(s) 82, 255, 390, 413
HpyCH4III ACNGT 2 cut(s) 11, 363
HpyCH4V TGCA 2 cut(s) 24, 173
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 298
HpyF3I CTNAG 2 cut(s) 54, 399
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 9 cut(s) 109, 150, 159, 223, 281, 330, 338, 392, 429
MaeI CTAG 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 223, 330
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 109, 203, 206
MhlI GDGCHC 2 cut(s) 130, 432
MluCI AATT 5 cut(s) 119, 270, 354, 367, 388
MnlI CCTC 2 cut(s) 49, 219
MseI TTAA 2 cut(s) 267, 357
MwoI GCNNNNNNNGC 2 cut(s) 165, 298
NdeII GATC 1 cut(s) 376
NlaIV GGNNCC 2 cut(s) 129, 207
NspV TTCGAA 1 cut(s) 68
PfeI GAWTC 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 129, 207
PspPI GGNCC 1 cut(s) 347
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 170, 248
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 267, 357
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 347
ScaI AGTACT 1 cut(s) 34
SduI GDGCHC 2 cut(s) 130, 432
SfcI CTRYAG 1 cut(s) 171
SfuI TTCGAA 1 cut(s) 68
Sse9I AATT 5 cut(s) 119, 270, 354, 367, 388
SspMI CTAG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 11, 363
TaqI TCGA 1 cut(s) 68
TasI AATT 5 cut(s) 119, 270, 354, 367, 388
TatI WGTACW 1 cut(s) 32
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 2 cut(s) 267, 357
Tru9I TTAA 2 cut(s) 267, 357
TspDTI ATGAA 1 cut(s) 327
Van91I CCANNNNNTGG 1 cut(s) 390
XapI RAATTY 1 cut(s) 119
XspI CTAG 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.