FvH4_6g18810

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004049
Physical Location & Seq
Reverse (-)
12380917 .. 12381936
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18810.t1

Sequence Viewer

Length: 606 bp
ATGAACTATGAGGGGAAGGTTGTAATCATCTCCTTTATATATCACTCTTATTTCCACCACCCTCAATCCTTCCCAACTTTCAAAACTAAAGTGTTGTCATCAAGCTTTGATAAAAGAGAAGCAATGGGAAATTGCCTGGTTCTGCAACAAAATGTTACAAAGATAATGAAACCAGATGGGAAAGTCCTGGAGTACAGAGCATCCATAAAAGTTTACCAGGTGTTGTCCGAGTTTTCTGGCTTTGCAATATCTGAGACGACTCTGGGGTACCAGCATCTCCGGCCAGATACCAAGCTGCTCGGCGGTCGCCTGTACTATCTTGTACCACTTCCTTTGCCATCAAAGAAGGCATCACAGAAGAAGGTGAGGTTTTCAATTGATCCTGAAGGTGAAGCTCAACAAGAAACTAAAGTTGTAAGAATTAAGCTAGTTATAAGTAAGCAACAACTGCAGGAGATGTTGGGAAAGGGAGCGGTTTCAGTTGATGACATGGTTTCCAGAATTCAATGTCAAACTAGCGTAGATAAGAAGACTGACAGCTTCGACAACAATGGTAGCTGTGAAGGGTGGAAACCTGTTTTAGAAAGCATACCTGAAGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

202

Amino Acids

22.73

Weight (kDa)

9.12

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 42 - 199 1.1e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 473
AciI CCGC 2 cut(s) 303, 473
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 4 cut(s) 194, 269, 314, 324
AgsI TTSAA 3 cut(s) 82, 375, 506
AjnI CCWGG 3 cut(s) 135, 186, 216
AluBI AGCT 6 cut(s) 105, 295, 395, 427, 540, 558
AluI AGCT 6 cut(s) 105, 295, 395, 427, 540, 558
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 1 cut(s) 374
AoxI GGCC 1 cut(s) 281
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 1 cut(s) 501
Asp718I GGTACC 1 cut(s) 267
AsuHPI GGTGA 2 cut(s) 376, 401
BanI GGYRCC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 536
BbvI GCAGC 1 cut(s) 282
BccI CCATC 2 cut(s) 170, 346
BciT130I CCWGG 3 cut(s) 137, 188, 218
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 3 cut(s) 428, 516, 604
BfmI CTRYAG 1 cut(s) 449
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 3 cut(s) 137, 188, 218
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 3 cut(s) 137, 188, 218
BmsI GCATC 3 cut(s) 209, 283, 359
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 3 cut(s) 137, 188, 218
BseGI GGATG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 1 cut(s) 243
BseXI GCAGC 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 1 cut(s) 283
BshNI GGYRCC 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 280
BsmAI GTCTC 1 cut(s) 248
BsmBI CGTCTC 1 cut(s) 248
BsnI GGCC 1 cut(s) 283
Bsp143I GATC 1 cut(s) 379
BspACI CCGC 2 cut(s) 303, 473
BspANI GGCC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 269
BspMAI CTGCAG 1 cut(s) 453
BspPI GGATC 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 267
BsrBI CCGCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 129
BssMI GATC 1 cut(s) 379
Bst2UI CCWGG 3 cut(s) 137, 188, 218
BstAPI GCANNNNNTGC 1 cut(s) 448
BstDEI CTNAG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 200
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 2 cut(s) 280, 448
BstNI CCWGG 3 cut(s) 137, 188, 218
BstSCI CCNGG 3 cut(s) 135, 186, 216
BstSFI CTRYAG 1 cut(s) 449
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 536
BsuRI GGCC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 216
Csp6I GTAC 4 cut(s) 193, 268, 313, 323
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 1 cut(s) 490
CviJI RGCY 8 cut(s) 105, 240, 283, 295, 395, 427, 540, 558
CviKI_1 RGCY 8 cut(s) 105, 240, 283, 295, 395, 427, 540, 558
CviQI GTAC 4 cut(s) 193, 268, 313, 323
DdeI CTNAG 1 cut(s) 252
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 501
EcoRII CCWGG 3 cut(s) 135, 186, 216
Esp3I CGTCTC 1 cut(s) 248
FaeI CATG 1 cut(s) 493
FaiI YATR 7 cut(s) 9, 38, 40, 206, 434, 491, 590
FatI CATG 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 3 cut(s) 428, 516, 604
GluI GCNGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 1 cut(s) 283
HapII CCGG 1 cut(s) 280
Hin1II CATG 1 cut(s) 493
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 1 cut(s) 259
HpaII CCGG 1 cut(s) 280
HphI GGTGA 2 cut(s) 376, 401
Hpy166II GTNNAC 2 cut(s) 214, 601
Hpy188I TCNGA 2 cut(s) 229, 253
Hpy188III TCNNGA 2 cut(s) 383, 498
Hpy8I GTNNAC 2 cut(s) 214, 601
HpyAV CCTTC 6 cut(s) 10, 79, 340, 355, 380, 557
HpyCH4V TGCA 3 cut(s) 145, 245, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 448
HpyF3I CTNAG 1 cut(s) 252
Hsp92II CATG 1 cut(s) 493
KpnI GGTACC 1 cut(s) 271
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 470
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 3 cut(s) 209, 283, 359
MabI ACCWGGT 1 cut(s) 216
MaeI CTAG 3 cut(s) 428, 516, 604
MaeIII GTNAC 1 cut(s) 154
MalI GATC 1 cut(s) 381
MbiI CCGCTC 1 cut(s) 473
MboI GATC 1 cut(s) 379
MboII GAAGA 2 cut(s) 370, 541
MfeI CAATTG 1 cut(s) 375
MluCI AATT 4 cut(s) 130, 375, 420, 501
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 3 cut(s) 4, 72, 360
MseI TTAA 1 cut(s) 423
MspI CCGG 1 cut(s) 280
MspR9I CCNGG 3 cut(s) 137, 188, 218
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 3 cut(s) 137, 188, 218
MwoI GCNNNNNNNGC 2 cut(s) 280, 448
NdeII GATC 1 cut(s) 379
NlaIII CATG 1 cut(s) 493
NlaIV GGNNCC 1 cut(s) 269
NmeAIII GCCGAG 1 cut(s) 279
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PsiI TTATAA 1 cut(s) 434
Psp6I CCWGG 3 cut(s) 135, 186, 216
PspGI CCWGG 3 cut(s) 135, 186, 216
PspN4I GGNNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 453
RsaI GTAC 4 cut(s) 194, 269, 314, 324
RsaNI GTAC 4 cut(s) 193, 268, 313, 323
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 1 cut(s) 379
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 3 cut(s) 137, 188, 218
SexAI ACCWGGT 1 cut(s) 216
SfaNI GCATC 3 cut(s) 209, 283, 359
SfcI CTRYAG 1 cut(s) 449
Sse9I AATT 4 cut(s) 130, 375, 420, 501
SsiI CCGC 2 cut(s) 303, 473
SspMI CTAG 3 cut(s) 428, 516, 604
StyD4I CCNGG 3 cut(s) 135, 186, 216
TaqI TCGA 1 cut(s) 543
TasI AATT 4 cut(s) 130, 375, 420, 501
TatI WGTACW 2 cut(s) 192, 312
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 17, 182
XapI RAATTY 1 cut(s) 501
XspI CTAG 3 cut(s) 428, 516, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.