pycom01g23580

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004049
Physical Location & Seq
Reverse (-)
20852815 .. 20853237
423 bp
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UTR
Exon/CDS
Intron
pycom01g23580.1

Sequence Viewer

Length: 423 bp
ATGGGCAACTGTTTGGTTCTCTCCAAGGAGGACAAAGTGGTGAGGGTTTTGAAGACAGATGGGAAGGTTTTAGAATTCAGGGCACCAACCCTAGTTAAGGACATTTTATCGAACTTTTCAGGCGTCGGTATTAGTTTATCAAAGCAAGCTTCGGAGCAGCTTTCTCCTGATTGCAAACTGAAGCTCGGGAAGGTTTACTATATGATTCCCCTTCCGGGTTCTGCAGAAAATGATAGTGCTGCTACAAGAAGGATTAAGATTGTCGTAACAAAGCAACAGCTTGAAGACCTACTGACTAAGCAAATATCAGTGCAGGAAGCACTTTCAGCATCAGGACCTGAAAAGAAAGCTTCTTCTTCTTGTGTTGATTCTTCAAAAATTTGGAAACCGAAGCTCAAATCCATTCCCGAAGAAATCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

141

Amino Acids

15.28

Weight (kDa)

9.11

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 137 9.7e-19 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AcsI RAATTY 2 cut(s) 74, 378
AcuI CTGAAG 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 97, 215
AgsI TTSAA 3 cut(s) 52, 284, 375
AluBI AGCT 6 cut(s) 149, 160, 184, 280, 350, 394
AluI AGCT 6 cut(s) 149, 160, 184, 280, 350, 394
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 157, 239
ApoI RAATTY 2 cut(s) 74, 378
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 52
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 82
BbsI GAAGAC 2 cut(s) 59, 291
BbvI GCAGC 2 cut(s) 169, 226
BccI CCATC 1 cut(s) 53
BcnI CCSGG 1 cut(s) 216
BfaI CTAG 1 cut(s) 92
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 2 cut(s) 158, 240
BlsI GCNGC 2 cut(s) 159, 241
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 1 cut(s) 338
BpiI GAAGAC 2 cut(s) 59, 291
BpuMI CCSGG 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 215
BseDI CCNNGG 1 cut(s) 24
BseLI CCNNNNNNNGG 2 cut(s) 97, 215
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 2 cut(s) 169, 226
BsgI GTGCAG 1 cut(s) 332
BshNI GGYRCC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 97, 215
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 84
BspMAI CTGCAG 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 24
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 1 cut(s) 297
BstENI CCTNNNNNAGG 1 cut(s) 95
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 214
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 169, 226
BstV2I GAAGAC 2 cut(s) 59, 291
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 147
CaiI CAGNNNCTG 1 cut(s) 338
Cfr13I GGNCC 1 cut(s) 335
CseI GACGC 1 cut(s) 112
CviJI RGCY 6 cut(s) 149, 160, 184, 280, 350, 394
CviKI_1 RGCY 6 cut(s) 149, 160, 184, 280, 350, 394
DdeI CTNAG 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 200
Eco88I CYCGRG 1 cut(s) 185
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaiI YATR 2 cut(s) 201, 203
Fnu4HI GCNGC 2 cut(s) 158, 240
Fsp4HI GCNGC 2 cut(s) 158, 240
FspBI CTAG 1 cut(s) 92
GluI GCNGC 2 cut(s) 158, 240
HapII CCGG 1 cut(s) 215
HgaI GACGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 123
HindIII AAGCTT 2 cut(s) 147, 348
HinfI GANTC 2 cut(s) 205, 368
HpaII CCGG 1 cut(s) 215
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 4 cut(s) 167, 187, 333, 407
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 4 cut(s) 58, 184, 221, 243
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 174, 224, 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 1 cut(s) 297
Hsp92I GRCGYC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 7 cut(s) 64, 105, 180, 228, 299, 318, 351
Lsp1109I GCAGC 2 cut(s) 169, 226
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 265
MboII GAAGA 6 cut(s) 64, 296, 345, 348, 363, 422
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 2 cut(s) 74, 378
MnlI CCTC 2 cut(s) 22, 36
MseI TTAA 2 cut(s) 96, 255
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 326
NciI CCSGG 1 cut(s) 216
NlaIV GGNNCC 1 cut(s) 84
PfeI GAWTC 2 cut(s) 205, 368
PkrI GCNGC 2 cut(s) 159, 241
PpuMI RGGWCCY 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 84
PspPI GGNCC 1 cut(s) 335
PspPPI RGGWCCY 1 cut(s) 335
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 338
SaqAI TTAA 2 cut(s) 96, 255
SatI GCNGC 2 cut(s) 158, 240
Sau96I GGNCC 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 85
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 1 cut(s) 335
Sse9I AATT 2 cut(s) 74, 378
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 110, 417
TasI AATT 2 cut(s) 74, 378
TfiI GAWTC 2 cut(s) 205, 368
Tru1I TTAA 2 cut(s) 96, 255
Tru9I TTAA 2 cut(s) 96, 255
TscAI CASTG 1 cut(s) 315
TseI GCWGC 2 cut(s) 157, 239
TspRI CASTG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 335
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 2 cut(s) 74, 378
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.