RchiOBHm_Chr1g0382771

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004049
Physical Location & Seq
Reverse (-)
67430892 .. 67431500
609 bp
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UTR
Exon/CDS
Intron
PRQ60571

Sequence Viewer

Length: 486 bp
ATGGGCAACTGTTTTGTTCTTTGCAGAATCAGTACTGCTTCTCGGATTGTTTCTGAGGATGTTGCGCCGAAGCAAGGAAAAGTTGTGAAGGTTCTGAAGATGGACGGGGAAGTTTTAGAATTCAGGGCACCTATTCTTGTCAAGAATGTCTTGTTGAGCTTTTCAGGCTCTGCAGGTATAGGTGTATCAAAGCAATTAGCTTCAGAACATCTCCCTGCAAATTATGAACTGAAGTTAGGGAAGTTTTACTATATGGTTCCTCTTCTTGGTTCTGTTACAAAAAACAACAGGACAGCTTCTGCTACAACAAGAAGGATTAAAATTGTAATAACTACGCAACAGCTTCAGGAGCTTTTGACAAACCAAGTATCAGTCCAGAAGTTACTTTCATTTTCAGGGCCGAAAATTAAACCGTGTAATTTGGTTGATCCTTCCACAAATTGGAAGCCTAAGCTTCAGGTCATCCCTGAAGAAAGCGAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

161

Amino Acids

17.74

Weight (kDa)

9.65

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 157 6.3e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 441
AclWI GGATC 1 cut(s) 422
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 5 cut(s) 116, 186, 251, 329, 440
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 3 cut(s) 74, 266, 441
AluBI AGCT 6 cut(s) 159, 200, 296, 343, 352, 454
AluI AGCT 6 cut(s) 159, 200, 296, 343, 352, 454
Alw21I GWGCWC 1 cut(s) 483
AlwI GGATC 1 cut(s) 422
AlwNI CAGNNNCTG 2 cut(s) 170, 299
AoxI GGCC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 119
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 483
BccI CCATC 1 cut(s) 94
BfmI CTRYAG 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 164
BmcAI AGTACT 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 2 cut(s) 129, 258
Bpu10I CCTNAGC 1 cut(s) 450
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 266, 441
BseGI GGATG 2 cut(s) 64, 462
BseLI CCNNNNNNNGG 3 cut(s) 74, 266, 441
BseMII CTCAG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 130
BshFI GGCC 1 cut(s) 400
BshNI GGYRCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 483
BslI CCNNNNNNNGG 3 cut(s) 74, 266, 441
BsnI GGCC 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 130, 483
Bsp143I GATC 1 cut(s) 427
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 129, 258
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 127
BssMI GATC 1 cut(s) 427
Bst4CI ACNGT 2 cut(s) 11, 414
Bst6I CTCTTC 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 2 cut(s) 54, 450
BstF5I GGATG 2 cut(s) 64, 462
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 1 cut(s) 430
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 2 cut(s) 165, 349
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 130
BsuRI GGCC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 64, 462
BtsIMutI CAGTG 1 cut(s) 481
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 479
CaiI CAGNNNCTG 2 cut(s) 170, 299
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 398
Csp6I GTAC 1 cut(s) 33
CviJI RGCY 9 cut(s) 159, 168, 200, 296, 343, 352, 400, 448, 454
CviKI_1 RGCY 9 cut(s) 159, 168, 200, 296, 343, 352, 400, 448, 454
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 2 cut(s) 54, 450
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco57I CTGAAG 5 cut(s) 116, 186, 251, 329, 440
EcoRI GAATTC 1 cut(s) 119
FaiI YATR 4 cut(s) 179, 225, 252, 254
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FokI GGATG 2 cut(s) 71, 449
GlaI GCGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 400
HhaI GCGC 1 cut(s) 67
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 1 cut(s) 27
Hpy188I TCNGA 4 cut(s) 45, 55, 96, 205
Hpy188III TCNNGA 3 cut(s) 142, 347, 376
HpyAV CCTTC 3 cut(s) 82, 306, 441
HpyCH4III ACNGT 2 cut(s) 11, 414
HpyCH4V TGCA 3 cut(s) 24, 173, 218
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 349
HpyF3I CTNAG 2 cut(s) 54, 450
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 427
LmnI GCTCC 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 274, 381
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 3 cut(s) 109, 254, 482
MhlI GDGCHC 2 cut(s) 130, 483
MluCI AATT 7 cut(s) 119, 194, 220, 321, 405, 418, 439
MnlI CCTC 2 cut(s) 49, 270
MseI TTAA 2 cut(s) 318, 408
MwoI GCNNNNNNNGC 2 cut(s) 165, 349
NdeII GATC 1 cut(s) 427
NlaIV GGNNCC 2 cut(s) 129, 258
PfeI GAWTC 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 441
PspN4I GGNNCC 2 cut(s) 129, 258
PspPI GGNCC 1 cut(s) 398
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 170, 299
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 318, 408
Sau3AI GATC 1 cut(s) 427
Sau96I GGNCC 1 cut(s) 398
ScaI AGTACT 1 cut(s) 34
SduI GDGCHC 2 cut(s) 130, 483
SfcI CTRYAG 1 cut(s) 171
Sse9I AATT 7 cut(s) 119, 194, 220, 321, 405, 418, 439
TaaI ACNGT 2 cut(s) 11, 414
TasI AATT 7 cut(s) 119, 194, 220, 321, 405, 418, 439
TatI WGTACW 1 cut(s) 32
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 2 cut(s) 318, 408
Tru9I TTAA 2 cut(s) 318, 408
TspDTI ATGAA 2 cut(s) 240, 378
Van91I CCANNNNNTGG 1 cut(s) 441
XapI RAATTY 1 cut(s) 119
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.