MD14G1023400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004049
Physical Location & Seq
Reverse (-)
2088709 .. 2089209
501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1023400.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGGGAATTGCTTGGTTATGCAAGAAAATGTTACAAAGATTATGAGACCTGATGGGAAGATCCTAGAGTACAGCGCAGACATGAAAGTTTACCAGGTGCTGTCGGAGTTTTCGGGGCATGCAATATCTGAGACGGCTCCAAACTACCAGCATCTCCGGCCAGACTCCAAGCTGCTTGGTGGTCACCTCTACTATCTCGTACCTCTCCCTTTGCCACCGCCCAAAAAGGCTTCCCAGAAGAAGGTGAGATTTTCGGAGCCTGAAGCCGAACGGCCTGAACCTGAACAAGAAACTAAAGTTGTAAGGATTAAGTTGGTTATAAGCAAGAAAGAATTGCAGGAGATGTTGACAAAGGGAGGAGTTGCAGTTCTTGATGAAATGGTTTCTCGGCTTCAAAGTGAAGAAAAAGGCATAGATAAGAGTAACAGTAACAGCTTCAACGTTGATGGTAACTGCGAAGGGTGGAAGCCTGCTCTCGAAAGCATACCTGAAGTAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

167

Amino Acids

18.61

Weight (kDa)

5.98

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 164 2.9e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
AciI CCGC 1 cut(s) 218
AclI AACGTT 1 cut(s) 441
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 2 cut(s) 71, 201
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 2 cut(s) 395, 439
AjnI CCWGG 1 cut(s) 93
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 2 cut(s) 172, 435
AluI AGCT 2 cut(s) 172, 435
Alw26I GTCTC 2 cut(s) 40, 125
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 100
AoxI GGCC 2 cut(s) 158, 272
ApeKI GCWGC 1 cut(s) 172
AspLEI GCGC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 176, 256
BbvI GCAGC 1 cut(s) 159
BccI CCATC 2 cut(s) 47, 440
BceAI ACGGC 2 cut(s) 150, 287
BciT130I CCWGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 40, 125
BfaI CTAG 2 cut(s) 65, 499
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme1390I CCNGG 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 138, 258
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bsc4I CCNNNNNNNGG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 120
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 2 cut(s) 160, 274
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 2 cut(s) 40, 125
BsmBI CGTCTC 1 cut(s) 125
BsnI GGCC 2 cut(s) 160, 274
Bso31I GGTCTC 1 cut(s) 40
Bsp143I GATC 1 cut(s) 60
BspACI CCGC 1 cut(s) 218
BspANI GGCC 2 cut(s) 160, 274
BspCNI CTCAG 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 138, 258
BspPI GGATC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 40
BssMI GATC 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 120, 471
BstDEI CTNAG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 182
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 1 cut(s) 63
BstMAI GTCTC 2 cut(s) 40, 125
BstMBI GATC 1 cut(s) 60
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BsuRI GGCC 2 cut(s) 160, 274
Cac8I GCNNGC 2 cut(s) 120, 471
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 1 cut(s) 77
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 2 cut(s) 70, 200
CviAII CATG 2 cut(s) 82, 119
CviQI GTAC 2 cut(s) 70, 200
DdeI CTNAG 1 cut(s) 129
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 282
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 2 cut(s) 85, 122
FaiI YATR 7 cut(s) 20, 44, 83, 120, 320, 413, 485
FatI CATG 2 cut(s) 81, 118
Fnu4HI GCNGC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 173
FspBI CTAG 2 cut(s) 65, 499
GlaI GCGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 173
HaeIII GGCC 2 cut(s) 160, 274
HapII CCGG 1 cut(s) 157
HhaI GCGC 1 cut(s) 77
Hin1II CATG 2 cut(s) 85, 122
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 1 cut(s) 164
HpaII CCGG 1 cut(s) 157
HphI GGTGA 2 cut(s) 176, 256
Hpy166II GTNNAC 2 cut(s) 91, 348
Hpy188I TCNGA 3 cut(s) 106, 130, 256
Hpy188III TCNNGA 2 cut(s) 371, 476
Hpy8I GTNNAC 2 cut(s) 91, 348
HpyAV CCTTC 2 cut(s) 235, 452
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 4 cut(s) 22, 122, 337, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 2 cut(s) 85, 122
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 1 cut(s) 60
LmnI GCTCC 2 cut(s) 142, 256
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 1 cut(s) 160
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 2 cut(s) 65, 499
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 5 cut(s) 31, 182, 422, 428, 449
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MboII GAAGA 3 cut(s) 70, 250, 413
MflI RGATCY 1 cut(s) 60
MluCI AATT 2 cut(s) 7, 332
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 3 cut(s) 197, 213, 350
MseI TTAA 1 cut(s) 309
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 1 cut(s) 60
NlaIII CATG 2 cut(s) 85, 122
NlaIV GGNNCC 2 cut(s) 138, 258
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 182
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PkrI GCNGC 1 cut(s) 174
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PsiI TTATAA 1 cut(s) 320
Psp1406I AACGTT 1 cut(s) 441
Psp6I CCWGG 1 cut(s) 93
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 138, 258
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 60
RsaI GTAC 2 cut(s) 71, 201
RsaNI GTAC 2 cut(s) 70, 200
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 1 cut(s) 60
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 95
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 1 cut(s) 160
SphI GCATGC 1 cut(s) 122
Sse9I AATT 2 cut(s) 7, 332
SsiI CCGC 1 cut(s) 218
SspMI CTAG 2 cut(s) 65, 499
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 1 cut(s) 444
TaqI TCGA 1 cut(s) 477
TasI AATT 2 cut(s) 7, 332
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 98, 390
XceI RCATGY 1 cut(s) 122
XspI CTAG 2 cut(s) 65, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.