AT3G10120

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004049
Physical Location & Seq
Forward (+)
3130072 .. 3130891
820 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10120.1

Sequence Viewer

Length: 522 bp
ATGGGGAACTGTTTAGTAATGGAGAAGAAAGTGATAAAGATAATGAGAAATGATGGGAAAGTAGTTGAATACAGAGGACCTATGAAGGTTCATCACATCCTCACCCAATTCTCTCCTCACTACTCTCTCTTTGACTCTCTCACCAACAATTGTCATCTTCATCCCCAAGCCAAGCTTCTCTGTGGCCGTCTCTACTATCTATTGCCCCAGGAGACTAACTCAATCAAGCACATGAAGAAGACGATGAAGAAAGTCAGGTTTGCAAATCCAGAGGTGGAAAAAGAAGAACAAGAAGAAGATAGATTAACAGATTGTTGTGACAACACCAAGGAGAAGACTAATGGTGTTGTGAGAGTGAAGATGGTTGTGAGTAAGCAAGAGCTGGAGAAGCTGCTTCAAGGAGGTTCTGTTCATGAAATGGTTTATAGGACTCTTGCTAAGCAACATCTGTGTGATGATGATGATGAGTGTCATAAAGAAGGCTGGAGACCTTTGTTAGATAGCATTCCTGAAACTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

173

Amino Acids

20.15

Weight (kDa)

7.1

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 171 2e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 184
AgsI TTSAA 2 cut(s) 68, 398
AjnI CCWGG 1 cut(s) 207
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AloI GAACNNNNNNTCC 2 cut(s) 393, 425
AluBI AGCT 3 cut(s) 175, 382, 391
AluI AGCT 3 cut(s) 175, 382, 391
Alw26I GTCTC 3 cut(s) 194, 206, 481
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 391
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 94, 133
AvaII GGWCC 1 cut(s) 77
BbsI GAAGAC 2 cut(s) 245, 341
BbvI GCAGC 1 cut(s) 378
BccI CCATC 2 cut(s) 47, 355
BceAI ACGGC 1 cut(s) 171
BciT130I CCWGG 1 cut(s) 209
BcoDI GTCTC 3 cut(s) 194, 206, 481
BisI GCNGC 1 cut(s) 392
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 393
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 209
BpiI GAAGAC 2 cut(s) 245, 341
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 2 cut(s) 404, 505
Bpu1102I GCTNAGC 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 207, 327
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 207, 327
BseGI GGATG 2 cut(s) 96, 160
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 378
BshFI GGCC 1 cut(s) 186
BsmAI GTCTC 3 cut(s) 194, 206, 481
BsmBI CGTCTC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 1 cut(s) 186
Bso31I GGTCTC 1 cut(s) 481
Bsp1720I GCTNAGC 1 cut(s) 438
BspANI GGCC 1 cut(s) 186
BspHI TCATGA 1 cut(s) 412
BspTNI GGTCTC 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 207, 327
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 11
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 96, 160
BstMAI GTCTC 3 cut(s) 194, 206, 481
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 378
BstV2I GAAGAC 2 cut(s) 245, 341
BsuRI GGCC 1 cut(s) 186
BtsCI GGATG 2 cut(s) 96, 160
CciI TCATGA 1 cut(s) 412
Cfr13I GGNCC 1 cut(s) 77
CviAII CATG 2 cut(s) 232, 413
CviJI RGCY 6 cut(s) 170, 175, 186, 382, 391, 483
CviKI_1 RGCY 6 cut(s) 170, 175, 186, 382, 391, 483
DdeI CTNAG 1 cut(s) 438
EaeI YGGCCR 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 481
Eco47I GGWCC 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 2 cut(s) 235, 416
FaiI YATR 5 cut(s) 83, 233, 414, 426, 474
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 2 cut(s) 231, 412
Fnu4HI GCNGC 1 cut(s) 392
FokI GGATG 2 cut(s) 83, 147
Fsp4HI GCNGC 1 cut(s) 392
GluI GCNGC 1 cut(s) 392
GsuI CTGGAG 2 cut(s) 404, 505
HaeIII GGCC 1 cut(s) 186
Hin1II CATG 2 cut(s) 235, 416
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 2 cut(s) 134, 430
HphI GGTGA 2 cut(s) 94, 133
Hpy188III TCNNGA 3 cut(s) 269, 413, 509
HpyAV CCTTC 2 cut(s) 79, 473
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 1 cut(s) 438
Hsp92II CATG 2 cut(s) 235, 416
LpnPI CCDG 6 cut(s) 194, 221, 241, 282, 368, 469
Lsp1109I GCAGC 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 317
MfeI CAATTG 1 cut(s) 148
MluCI AATT 2 cut(s) 107, 148
MlyI GAGTC 2 cut(s) 128, 424
MnlI CCTC 5 cut(s) 68, 110, 126, 265, 395
MseI TTAA 1 cut(s) 305
MslI CAYNNNNRTG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 209
MunI CAATTG 1 cut(s) 148
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 1 cut(s) 388
NlaIII CATG 2 cut(s) 235, 416
NmuCI GTSAC 1 cut(s) 317
PagI TCATGA 1 cut(s) 412
PctI GAATGC 1 cut(s) 504
PkrI GCNGC 1 cut(s) 393
PleI GAGTC 2 cut(s) 128, 424
PpsI GAGTC 2 cut(s) 128, 424
PpuMI RGGWCCY 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspPI GGNCC 1 cut(s) 77
PspPPI RGGWCCY 1 cut(s) 77
RseI CAYNNNNRTG 1 cut(s) 450
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 1 cut(s) 392
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 2 cut(s) 128, 424
ScrFI CCNGG 1 cut(s) 209
SetI ASST 9 cut(s) 82, 90, 177, 260, 276, 384, 393, 406, 493
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 450
Sse9I AATT 2 cut(s) 107, 148
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 11
TasI AATT 2 cut(s) 107, 148
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 1 cut(s) 391
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 7 cut(s) 80, 98, 149, 248, 260, 401, 429
VpaK11BI GGWCC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.