Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004514

Physical Location & Seq

Genomic Coordinates Reverse (-)

5089975 .. 5096627

Gene Structure 6653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1045900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGGAGAAACCAAACTCATCTGGGTTGGCAAGGACCAAGTCTGAACAATTGGTGGAAACTATAGCCGCGGAGTTGAAGTCACCTCCGCCGGGCGAAGCCACTTCGACGGTTGAAGGCGGATCAACACTGTCGAGGAAGTCAAGCCGGCGCATGATGGGGGCGTCACCCGGGCGTGGTAGTGGCAGTGTTGGCAAAAACACACACATCAGGAAGTCCAGGAGCGCCCAAATGAAGCTGGACATGGATGAGATCAGCAGCGGCGCTGCCTTAAGCCGCGCCTCTAGCGCCAGCTTAGGATTTTCGTTCTCTTTCACCGGCTTCACTGTGCCTGCTGATGATATCGCCGACACAAAGCCTTTCAGCGACGACGATGATTTACCAGAAGATCTTGAAGCTGGCATCACCAGGAAGCCAAAATTCCAAACAGAACCCACACTGCCATTATATCTCAAGTTCACAGATGTGACATACAAGGTCATTTTCAAAGGAATGAGGACAACTGAAGAGAAGTATATCTTGAACGGGATTGCTGGTTTGGTTAACCCTGGTGAAGTTTTGGCCCTGATGGGACCATCAGGAAGTGGAAAGACCACCCTCCTTAATCTGCTTGGAGGCAGGGGAAGCCAAGCCAATGTTGGCGGTTCAATTACTTACAATGATCAGCCATATTCCAAGTACCTAAAGAGAAAGATAGGGTTTGTGACTCAGGATGACGTTCTATTTCCTCACCTTACGGTGAAAGAGACATTGACATATGCAGCCCTCCTACGGCTGCCAAAGACATTGACAAAAGAGCAGAAGGAAAAACGAGCGATAGATGTCATCTGTGAGCTAGGCTTAGAGAGGTGCCAAGACACTATGATTGGTGGCTCCTTTGTCCGCGGGGTATCAGGTGGAGAGAGGAAGAGAGTTTGCATTGGCAATGAGATCCTAATCAACCCTTCTCTTCTGTTTCTGGATGAACCAACTTCCGGCTTGGATTCTACAACTGCACTGCGAATTGGTCAGATGTTACAAGACATAGCAGAGGCTGGCAAGACTGTGGTGACAACAATCCACCAGCCATCAAGTCGACTCTTCCACAAATTCGACAAGTTGATCCTTCTTGGGAAGGGAAGCTTGCTCTACTTTGGAAAAGCATCAGAAGCAATGGTGTACTTCTCATGCATAGGATGTTCTCCGCTTATTGCCATGAACCCAGCAGAGTTCTTGCTAGACCTTGCAAACGGAAATTTAAATGATGTTTCAGTACCTTCAGAATTAGAGGATAAAGTGCAAATGGGAAGTTCAGAAGCAGATGATACAAGAAATGGAAAGCCATCTCCAGCAGTTGTGCATGAGTATCTTGTGGAGTCATACGAGACACGAGTTGCAAACGAGGAGAAGAAGAAGCTTATGGTTCCCCTTCCCATCGATGAGGAACTGAAGTCAAAGATGTCATTTTCGAAGCGGGAATGGGGAACAAGCTGGTGGGAGCAATTTTCTATCTTGTTCTGGAGAGGAATTAAAGAAAGGAGGCATGACTACTTCAGCTGGTTGAGATTCACCCAAGTGCTCTCCACCGCAATAATTTTGGGCTTGCTCTGGTGGCAGTCAGATGCTAACAATCCCAAAGGCCGGGAGGATCAGGCAGGGCTGCTTTTCTTCATTTCTGTTTTCTGGGGATTTTTACCCGTCTTCACAGCAATCTTCACATTTCCTCAAGAAAGAGCAATGCTGGTGAAGGAACGATCGGCTGACATGTACAGATTAAGTGCATATTTTGCTTCTAGGACTACTAGTGACCTCCCACTTGATCTAATGCTACCAGTTATGTTCCTCGTCGTTGTATATTTCATGGCTGGCTTAAGGCCGAGTGCTGAAACCTTCTTCCTAAGCGTGCTTACAGTTTTCCTCTGCATTGTGGCAGCACAGGGACTTGGACTCGCTATCGGAGCTACATTGATGGACCTAAAGAAAGCCACAACTCTTGCGTCAGTAACTGTGATGACGTTCATGCTTGCTGGAGGGTTCTTTGTGAAAGAAGTTCCGGTGTTCATATCTTGGATTCGGTATATGTCTTTCGACTACCACACATACAGGATTCTTCTTAAGGTGCAGTACGAACACATCACACCGACTGTTCATGGGTTGAGCACAGACCGCGGTTTAACAGAAATTGGTGCCCTTATAGCCATGGTTTTTGGATACCGGCTCTTGGCATACCTTTCTTTGCGAAGAATGAAGCTTCAAGGTGGGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006833 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0010035 GO:0010148 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0042044 GO:0042221 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

748

Amino Acids

82.6

Weight (kDa)

8.58

Isoelectric Point (pI)

37.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	174 - 324	1.4e-28	ABC transporter
ABC2_membrane_7	PF19055	353 - 411	1.6e-06	ABC-2 type transporter
ABC2_membrane_7	PF19055	415 - 644	8.3e-07	ABC-2 type transporter
ABC2_membrane	PF01061	492 - 701	1.6e-46	ABC-2 type transporter
ABC2_membrane_3	PF12698	546 - 736	5.6e-06	ABC-2 family transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06530 AT5G06530 AT5G06530 AT5G06530
fragaria_vesca	FvH4_6g21010 FvH4_6g21010 FvH4_6g21010
malus_domestica	MD12G1045900.v1.1 MD14G1044500.v1.1
prunus_persica	Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1
pyrus_communis	pycom12g03940 pycom14g03770
rosa_chinensis	RchiOBHm_Chr3g0477391
rosa_laevigata	RLG00000023726
rosa_multiflora	Rmu_ssc0000341.1_g000030
rosa_roxburghii	Rroxscaffold_1G00008820 Rroxscaffold_3G00241160 Rroxscaffold_4G00299920 Rroxscaffold_6G00404230 Rroxscaffold_6G00404700
rosa_rugosa	Rorug03G0159900 Rorug03G0160000 Rorug03G0160100
rosa_samantha	Rh3AG210600 Rh3BG243800 Rh3CG237700 Rh3DG237400
rosa_wichuraiana	Rw3G019080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	846, 2162
AccI	GTMKAC	1 cut(s)	1072
AccII	CGCG	4 cut(s)	68, 276, 882, 2145
AclWI	GGATC	4 cut(s)	127, 922, 1093, 1632
AcsI	RAATTY	3 cut(s)	416, 1085, 1231
AcuI	CTGAAG	4 cut(s)	522, 1239, 1445, 1513
AcyI	GRCGYC	1 cut(s)	161
AfaI	GTAC	5 cut(s)	677, 1157, 1251, 1745, 2102
AfiI	CCNNNNNNNGG	6 cut(s)	89, 173, 768, 971, 1617, 2197
AflII	CTTAAG	3 cut(s)	268, 1846, 2090
AflIII	ACRYGT	1 cut(s)	1740
AgsI	TTSAA	7 cut(s)	76, 113, 392, 484, 520, 645, 2231
AhlI	ACTAGT	1 cut(s)	1778
AjnI	CCWGG	3 cut(s)	215, 404, 544
AleI	CACNNNNGTG	2 cut(s)	461, 1550
Alw21I	GWGCWC	2 cut(s)	1557, 2138
Alw26I	GTCTC	2 cut(s)	737, 1355
AlwI	GGATC	4 cut(s)	127, 922, 1093, 1632
AlwNI	CAGNNNCTG	2 cut(s)	1031, 1982
Ama87I	CYCGRG	1 cut(s)	167
AoxI	GGCC	3 cut(s)	558, 1615, 1850
ApeKI	GCWGC	6 cut(s)	255, 263, 758, 772, 1636, 1907
ApoI	RAATTY	3 cut(s)	416, 1085, 1231
AspLEI	GCGC	5 cut(s)	150, 224, 263, 278, 287
AspS9I	GGNCC	4 cut(s)	33, 559, 569, 1948
AsuC2I	CCSGG	4 cut(s)	90, 168, 169, 1619
AsuII	TTCGAA	1 cut(s)	1445
AvaI	CYCGRG	1 cut(s)	167
AvaII	GGWCC	3 cut(s)	33, 569, 1948
BaeGI	GKGCMC	1 cut(s)	2167
BanI	GGYRCC	2 cut(s)	846, 2162
BauI	CACGAG	1 cut(s)	1365
BbsI	GAAGAC	1 cut(s)	1669
Bbv12I	GWGCWC	2 cut(s)	1557, 2138
BbvI	GCAGC	6 cut(s)	250, 267, 759, 770, 1623, 1919
BccI	CCATC	7 cut(s)	148, 559, 580, 1072, 1327, 1418, 1939
BceAI	ACGGC	1 cut(s)	785
BciT130I	CCWGG	3 cut(s)	217, 406, 546
BciVI	GTATCC	1 cut(s)	2180
BclI	TGATCA	1 cut(s)	658
BcnI	CCSGG	4 cut(s)	90, 168, 169, 1619
BcoDI	GTCTC	2 cut(s)	737, 1355
BcuI	ACTAGT	1 cut(s)	1778
BfaI	CTAG	5 cut(s)	282, 833, 1214, 1770, 1779
BfmI	CTRYAG	1 cut(s)	60
BfoI	RGCGCY	3 cut(s)	225, 264, 288
BfrI	CTTAAG	3 cut(s)	268, 1846, 2090
BfuI	GTATCC	1 cut(s)	2180
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	9 cut(s)	66, 256, 259, 264, 274, 759, 773, 1637, 1908
BlsI	GCNGC	9 cut(s)	67, 257, 260, 265, 275, 760, 774, 1638, 1909
Bme1390I	CCNGG	7 cut(s)	90, 168, 169, 217, 406, 546, 1619
Bme18I	GGWCC	3 cut(s)	33, 569, 1948
BmeT110I	CYCGRG	1 cut(s)	167
BmgT120I	GGNCC	4 cut(s)	33, 559, 569, 1948
BmiI	GGNNCC	5 cut(s)	570, 848, 871, 1401, 2164
BmrFI	CCNGG	7 cut(s)	90, 168, 169, 217, 406, 546, 1619
BmsI	GCATC	3 cut(s)	408, 1148, 1588
BpiI	GAAGAC	1 cut(s)	1669
BpmI	CTGGAG	3 cut(s)	1308, 1516, 2025
Bpu10I	CCTNAGC	2 cut(s)	292, 1874
Bpu14I	TTCGAA	1 cut(s)	1445
BpuEI	CTTGAG	2 cut(s)	434, 1686
BpuMI	CCSGG	4 cut(s)	90, 168, 169, 1619
Bsa29I	ATCGAT	1 cut(s)	1413
BsaBI	GATNNNNATC	1 cut(s)	932
BsaHI	GRCGYC	1 cut(s)	161
BsaJI	CCNNGG	6 cut(s)	66, 167, 544, 880, 2143, 2175
BsaWI	WCCGGW	1 cut(s)	2029
BsaXI	ACNNNNNCTCC	2 cut(s)	62, 92
Bsc4I	CCNNNNNNNGG	6 cut(s)	89, 173, 768, 971, 1617, 2197
Bse118I	RCCGGY	3 cut(s)	144, 314, 2190
Bse1I	ACTGG	1 cut(s)	1808
Bse3DI	GCAATG	3 cut(s)	928, 1155, 1719
Bse8I	GATNNNNATC	1 cut(s)	932
BseBI	CCWGG	3 cut(s)	217, 406, 546
BseCI	ATCGAT	1 cut(s)	1413
BseDI	CCNNGG	6 cut(s)	66, 167, 544, 880, 2143, 2175
BseGI	GGATG	4 cut(s)	250, 715, 964, 1178
BseJI	GATNNNNATC	1 cut(s)	932
BseLI	CCNNNNNNNGG	6 cut(s)	89, 173, 768, 971, 1617, 2197
BseMI	GCAATG	3 cut(s)	928, 1155, 1719
BseMII	CTCAG	1 cut(s)	719
BseNI	ACTGG	1 cut(s)	1808
BseRI	GAGGAG	1 cut(s)	1394
BseSI	GKGCMC	1 cut(s)	2167
BseXI	GCAGC	6 cut(s)	250, 267, 759, 770, 1623, 1919
BseYI	CCCAGC	1 cut(s)	1198
BsgI	GTGCAG	2 cut(s)	975, 2117
Bsh1236I	CGCG	4 cut(s)	68, 276, 882, 2145
Bsh1285I	CGRYCG	1 cut(s)	1733
BshFI	GGCC	3 cut(s)	560, 1617, 1852
BshNI	GGYRCC	2 cut(s)	846, 2162
BshVI	ATCGAT	1 cut(s)	1413
BsiEI	CGRYCG	1 cut(s)	1733
BsiHKAI	GWGCWC	2 cut(s)	1557, 2138
BsiHKCI	CYCGRG	1 cut(s)	167
BsiSI	CCGG	8 cut(s)	89, 145, 168, 315, 972, 1618, 2030, 2191
BslFI	GGGAC	2 cut(s)	582, 1929
BslI	CCNNNNNNNGG	6 cut(s)	89, 173, 768, 971, 1617, 2197
BsmAI	GTCTC	2 cut(s)	737, 1355
BsmFI	GGGAC	2 cut(s)	582, 1929
BsnI	GGCC	3 cut(s)	560, 1617, 1852
BsoBI	CYCGRG	1 cut(s)	167
Bsp119I	TTCGAA	1 cut(s)	1445
Bsp1286I	GDGCHC	3 cut(s)	1557, 2138, 2167
Bsp1407I	TGTACA	1 cut(s)	1743
Bsp143I	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
Bsp19I	CCATGG	1 cut(s)	2175
BspANI	GGCC	3 cut(s)	560, 1617, 1852
BspCNI	CTCAG	1 cut(s)	718
BspDI	ATCGAT	1 cut(s)	1413
BspFNI	CGCG	4 cut(s)	68, 276, 882, 2145
BspLI	GGNNCC	5 cut(s)	570, 848, 871, 1401, 2164
BspPI	GGATC	4 cut(s)	127, 922, 1093, 1632
BspT104I	TTCGAA	1 cut(s)	1445
BspT107I	GGYRCC	2 cut(s)	846, 2162
BspTI	CTTAAG	3 cut(s)	268, 1846, 2090
BsrDI	GCAATG	3 cut(s)	928, 1155, 1719
BsrFI	RCCGGY	3 cut(s)	144, 314, 2190
BsrGI	TGTACA	1 cut(s)	1743
BsrI	ACTGG	1 cut(s)	1808
BssAI	RCCGGY	3 cut(s)	144, 314, 2190
BssECI	CCNNGG	6 cut(s)	66, 167, 544, 880, 2143, 2175
BssMI	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
BssNI	GRCGYC	1 cut(s)	161
BssSI	CACGAG	1 cut(s)	1365
BssT1I	CCWWGG	1 cut(s)	2175
Bst2BI	CACGAG	1 cut(s)	1365
Bst2UI	CCWGG	3 cut(s)	217, 406, 546
Bst4CI	ACNGT	8 cut(s)	109, 129, 325, 736, 1042, 1888, 1984, 2122
Bst6I	CTCTTC	4 cut(s)	498, 899, 951, 1082
BstACI	GRCGYC	1 cut(s)	161
BstAFI	CTTAAG	3 cut(s)	268, 1846, 2090
BstAPI	GCANNNNNTGC	1 cut(s)	1763
BstAUI	TGTACA	1 cut(s)	1743
BstBI	TTCGAA	1 cut(s)	1445
BstDEI	CTNAG	4 cut(s)	292, 705, 838, 1874
BstDSI	CCRYGG	4 cut(s)	66, 880, 2143, 2175
BstF5I	GGATG	4 cut(s)	250, 715, 964, 1178
BstFNI	CGCG	4 cut(s)	68, 276, 882, 2145
BstH2I	RGCGCY	3 cut(s)	225, 264, 288
BstHHI	GCGC	5 cut(s)	150, 224, 263, 278, 287
BstKTI	GATC	9 cut(s)	122, 252, 388, 661, 930, 1101, 1627, 1733, 1798
BstMAI	GTCTC	2 cut(s)	737, 1355
BstMBI	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
BstMCI	CGRYCG	1 cut(s)	1733
BstNI	CCWGG	3 cut(s)	217, 406, 546
BstNSI	RCATGY	1 cut(s)	1744
BstSCI	CCNGG	7 cut(s)	88, 166, 167, 215, 404, 544, 1617
BstSFI	CTRYAG	1 cut(s)	60
BstSLI	GKGCMC	1 cut(s)	2167
BstUI	CGCG	4 cut(s)	68, 276, 882, 2145
BstV1I	GCAGC	6 cut(s)	250, 267, 759, 770, 1623, 1919
BstV2I	GAAGAC	1 cut(s)	1669
BstX2I	RGATCY	2 cut(s)	385, 927
BstYI	RGATCY	2 cut(s)	385, 927
Bsu15I	ATCGAT	1 cut(s)	1413
BsuI	GTATCC	1 cut(s)	2180
BsuRI	GGCC	3 cut(s)	560, 1617, 1852
BsuTUI	ATCGAT	1 cut(s)	1413
BtgI	CCRYGG	4 cut(s)	66, 880, 2143, 2175
BtsCI	GGATG	4 cut(s)	250, 715, 964, 1178
BtsI	GCAGTG	3 cut(s)	190, 434, 992
BtsIMutI	CAGTG	5 cut(s)	125, 190, 321, 434, 992
CaiI	CAGNNNCTG	2 cut(s)	1031, 1982
CfoI	GCGC	5 cut(s)	150, 224, 263, 278, 287
Cfr10I	RCCGGY	3 cut(s)	144, 314, 2190
Cfr13I	GGNCC	4 cut(s)	33, 559, 569, 1948
Cfr42I	CCGCGG	3 cut(s)	69, 883, 2146
Cfr9I	CCCGGG	1 cut(s)	167
ClaI	ATCGAT	1 cut(s)	1413
CseI	GACGC	2 cut(s)	150, 1962
Csp6I	GTAC	5 cut(s)	676, 1156, 1250, 1744, 2101
CspCI	CAANNNNNGTGG	2 cut(s)	1951, 1986
CviQI	GTAC	5 cut(s)	676, 1156, 1250, 1744, 2101
DdeI	CTNAG	4 cut(s)	292, 705, 838, 1874
DpnI	GATC	9 cut(s)	121, 251, 387, 660, 929, 1100, 1626, 1732, 1797
DpnII	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
DraI	TTTAAA	1 cut(s)	1236
Eam1104I	CTCTTC	4 cut(s)	498, 899, 951, 1082
EarI	CTCTTC	4 cut(s)	498, 899, 951, 1082
EciI	GGCGGA	2 cut(s)	75, 132
Eco130I	CCWWGG	1 cut(s)	2175
Eco32I	GATATC	1 cut(s)	340
Eco47I	GGWCC	3 cut(s)	33, 569, 1948
Eco57I	CTGAAG	4 cut(s)	522, 1239, 1445, 1513
Eco88I	CYCGRG	1 cut(s)	167
EcoRII	CCWGG	3 cut(s)	215, 404, 544
EcoRV	GATATC	1 cut(s)	340
EcoT14I	CCWWGG	1 cut(s)	2175
EcoT22I	ATGCAT	1 cut(s)	1169
ErhI	CCWWGG	1 cut(s)	2175
FalI	AAGNNNNNCTT	4 cut(s)	500, 532, 1103, 1135
FaqI	GGGAC	2 cut(s)	582, 1929
FauI	CCCGC	2 cut(s)	875, 1443
FauNDI	CATATG	1 cut(s)	754
FbaI	TGATCA	1 cut(s)	658
FblI	GTMKAC	1 cut(s)	1072
Fnu4HI	GCNGC	9 cut(s)	66, 256, 259, 264, 274, 759, 773, 1637, 1908
FokI	GGATG	4 cut(s)	257, 722, 971, 1185
Fsp4HI	GCNGC	9 cut(s)	66, 256, 259, 264, 274, 759, 773, 1637, 1908
FspBI	CTAG	5 cut(s)	282, 833, 1214, 1770, 1779
GlaI	GCGC	5 cut(s)	149, 223, 262, 277, 286
GluI	GCNGC	9 cut(s)	66, 256, 259, 264, 274, 759, 773, 1637, 1908
GsaI	CCCAGC	1 cut(s)	1202
GsuI	CTGGAG	3 cut(s)	1308, 1516, 2025
HaeII	RGCGCY	3 cut(s)	225, 264, 288
HaeIII	GGCC	3 cut(s)	560, 1617, 1852
HapII	CCGG	8 cut(s)	89, 145, 168, 315, 972, 1618, 2030, 2191
HgaI	GACGC	2 cut(s)	150, 1962
HhaI	GCGC	5 cut(s)	150, 224, 263, 278, 287
Hin1I	GRCGYC	1 cut(s)	161
Hin6I	GCGC	5 cut(s)	148, 222, 261, 276, 285
HinP1I	GCGC	5 cut(s)	148, 222, 261, 276, 285
HincII	GTYRAC	2 cut(s)	541, 1073
HindII	GTYRAC	2 cut(s)	541, 1073
HindIII	AAGCTT	3 cut(s)	1117, 1391, 2225
HinfI	GANTC	8 cut(s)	703, 980, 1074, 1352, 1542, 1923, 2047, 2083
HpaI	GTTAAC	1 cut(s)	541
HpaII	CCGG	8 cut(s)	89, 145, 168, 315, 972, 1618, 2030, 2191
Hpy166II	GTNNAC	4 cut(s)	456, 541, 1073, 1156
Hpy188I	TCNGA	7 cut(s)	43, 1008, 1144, 1258, 1291, 1597, 1934
Hpy188III	TCNNGA	8 cut(s)	208, 389, 517, 576, 707, 956, 1495, 1703
Hpy8I	GTNNAC	4 cut(s)	456, 541, 1073, 1156
Hpy99I	CGWCG	4 cut(s)	109, 368, 371, 1826
HpyAV	CCTTC	9 cut(s)	107, 793, 951, 1105, 1112, 1263, 1415, 1717, 1876
HpyCH4III	ACNGT	8 cut(s)	109, 129, 325, 736, 1042, 1888, 1984, 2122
HpyCH4IV	ACGT	2 cut(s)	714, 1991
HpyF3I	CTNAG	4 cut(s)	292, 705, 838, 1874
HpySE526I	ACGT	2 cut(s)	714, 1991
Hsp92I	GRCGYC	1 cut(s)	161
HspAI	GCGC	5 cut(s)	148, 222, 261, 276, 285
KroI	GCCGGC	1 cut(s)	144
KroNI	GCCGGC	1 cut(s)	146
Ksp22I	TGATCA	1 cut(s)	658
KspAI	GTTAAC	1 cut(s)	541
KspI	CCGCGG	3 cut(s)	69, 883, 2146
Kzo9I	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
LmnI	GCTCC	4 cut(s)	219, 875, 1474, 1934
Lsp1109I	GCAGC	6 cut(s)	250, 267, 759, 770, 1623, 1919
LweI	GCATC	3 cut(s)	408, 1148, 1588
MaeI	CTAG	5 cut(s)	282, 833, 1214, 1770, 1779
MaeII	ACGT	2 cut(s)	714, 1991
MaeIII	GTNAC	8 cut(s)	78, 162, 463, 700, 1011, 1045, 1781, 1978
MalI	GATC	9 cut(s)	121, 251, 387, 660, 929, 1100, 1626, 1732, 1797
MboI	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
MfeI	CAATTG	1 cut(s)	47
MflI	RGATCY	2 cut(s)	385, 927
MhlI	GDGCHC	3 cut(s)	1557, 2138, 2167
MlyI	GAGTC	4 cut(s)	697, 1068, 1361, 1917
Mph1103I	ATGCAT	1 cut(s)	1169
MroNI	GCCGGC	1 cut(s)	144
MseI	TTAA	9 cut(s)	269, 540, 600, 1235, 1506, 1751, 1847, 2091, 2150
MslI	CAYNNNNRTG	2 cut(s)	461, 1550
MspA1I	CMGCKG	5 cut(s)	68, 258, 882, 1533, 2145
MspCI	CTTAAG	3 cut(s)	268, 1846, 2090
MspI	CCGG	8 cut(s)	89, 145, 168, 315, 972, 1618, 2030, 2191
MspR9I	CCNGG	7 cut(s)	90, 168, 169, 217, 406, 546, 1619
MunI	CAATTG	1 cut(s)	47
MvaI	CCWGG	3 cut(s)	217, 406, 546
MvnI	CGCG	4 cut(s)	68, 276, 882, 2145
NaeI	GCCGGC	1 cut(s)	146
NciI	CCSGG	4 cut(s)	90, 168, 169, 1619
NcoI	CCATGG	1 cut(s)	2175
NdeI	CATATG	1 cut(s)	754
NdeII	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
NgoMIV	GCCGGC	1 cut(s)	144
NlaIV	GGNNCC	5 cut(s)	570, 848, 871, 1401, 2164
NmeAIII	GCCGAG	1 cut(s)	1878
NmuCI	GTSAC	6 cut(s)	78, 162, 463, 700, 1045, 1781
NsiI	ATGCAT	1 cut(s)	1169
NspI	RCATGY	1 cut(s)	1744
NspV	TTCGAA	1 cut(s)	1445
OliI	CACNNNNGTG	2 cut(s)	461, 1550
PciI	ACATGT	1 cut(s)	1740
PdiI	GCCGGC	1 cut(s)	146
PfeI	GAWTC	4 cut(s)	980, 1542, 2047, 2083
PflFI	GACNNNGTC	1 cut(s)	37
PfoI	TCCNGGA	1 cut(s)	215
PkrI	GCNGC	9 cut(s)	67, 257, 260, 265, 275, 760, 774, 1638, 1909
Ple19I	CGATCG	1 cut(s)	1733
PleI	GAGTC	4 cut(s)	697, 1068, 1360, 1917
PpsI	GAGTC	4 cut(s)	697, 1068, 1360, 1917
PscI	ACATGT	1 cut(s)	1740
Psp6I	CCWGG	3 cut(s)	215, 404, 544
PspFI	CCCAGC	1 cut(s)	1198
PspGI	CCWGG	3 cut(s)	215, 404, 544
PspN4I	GGNNCC	5 cut(s)	570, 848, 871, 1401, 2164
PspPI	GGNCC	4 cut(s)	33, 559, 569, 1948
PstNI	CAGNNNCTG	2 cut(s)	1031, 1982
PsuI	RGATCY	2 cut(s)	385, 927
PsyI	GACNNNGTC	1 cut(s)	37
PvuI	CGATCG	1 cut(s)	1733
PvuII	CAGCTG	1 cut(s)	1533
RsaI	GTAC	5 cut(s)	677, 1157, 1251, 1745, 2102
RsaNI	GTAC	5 cut(s)	676, 1156, 1250, 1744, 2101
RseI	CAYNNNNRTG	2 cut(s)	461, 1550
SacII	CCGCGG	3 cut(s)	69, 883, 2146
SalI	GTCGAC	1 cut(s)	1071
SaqAI	TTAA	9 cut(s)	269, 540, 600, 1235, 1506, 1751, 1847, 2091, 2150
SatI	GCNGC	9 cut(s)	66, 256, 259, 264, 274, 759, 773, 1637, 1908
Sau3AI	GATC	9 cut(s)	119, 249, 385, 658, 927, 1098, 1624, 1730, 1795
Sau96I	GGNCC	4 cut(s)	33, 559, 569, 1948
SchI	GAGTC	4 cut(s)	697, 1068, 1361, 1917
ScrFI	CCNGG	7 cut(s)	90, 168, 169, 217, 406, 546, 1619
SduI	GDGCHC	3 cut(s)	1557, 2138, 2167
SfaNI	GCATC	3 cut(s)	408, 1148, 1588
SfcI	CTRYAG	1 cut(s)	60
Sfr303I	CCGCGG	3 cut(s)	69, 883, 2146
SfuI	TTCGAA	1 cut(s)	1445
SgrBI	CCGCGG	3 cut(s)	69, 883, 2146
SinI	GGWCC	3 cut(s)	33, 569, 1948
SmaI	CCCGGG	1 cut(s)	169
SmiI	ATTTAAAT	1 cut(s)	1236
SmiMI	CAYNNNNRTG	2 cut(s)	461, 1550
SmlI	CTYRAG	5 cut(s)	268, 449, 1701, 1846, 2090
SmoI	CTYRAG	5 cut(s)	268, 449, 1701, 1846, 2090
SpeI	ACTAGT	1 cut(s)	1778
SspMI	CTAG	5 cut(s)	282, 833, 1214, 1770, 1779
StyD4I	CCNGG	7 cut(s)	88, 166, 167, 215, 404, 544, 1617
StyI	CCWWGG	1 cut(s)	2175
SwaI	ATTTAAAT	1 cut(s)	1236
TaaI	ACNGT	8 cut(s)	109, 129, 325, 736, 1042, 1888, 1984, 2122
TaiI	ACGT	2 cut(s)	717, 1994
TaqI	TCGA	7 cut(s)	104, 131, 1072, 1089, 1413, 1445, 2064
TatI	WGTACW	2 cut(s)	1155, 1743
TauI	GCSGC	3 cut(s)	68, 261, 276
TfiI	GAWTC	4 cut(s)	980, 1542, 2047, 2083
Tru1I	TTAA	9 cut(s)	269, 540, 600, 1235, 1506, 1751, 1847, 2091, 2150
Tru9I	TTAA	9 cut(s)	269, 540, 600, 1235, 1506, 1751, 1847, 2091, 2150
TscAI	CASTG	5 cut(s)	132, 190, 328, 441, 999
TseFI	GTSAC	6 cut(s)	78, 162, 463, 700, 1045, 1781
TseI	GCWGC	6 cut(s)	255, 263, 758, 772, 1636, 1907
Tsp45I	GTSAC	6 cut(s)	78, 162, 463, 700, 1045, 1781
TspDTI	ATGAA	9 cut(s)	245, 975, 1208, 1636, 1825, 1984, 2026, 2114, 2237
TspGWI	ACGGA	1 cut(s)	1242
TspMI	CCCGGG	1 cut(s)	167
TspRI	CASTG	5 cut(s)	132, 190, 328, 441, 999
Tth111I	GACNNNGTC	1 cut(s)	37
Vha464I	CTTAAG	3 cut(s)	268, 1846, 2090
VpaK11BI	GGWCC	3 cut(s)	33, 569, 1948
XapI	RAATTY	3 cut(s)	416, 1085, 1231
XceI	RCATGY	1 cut(s)	1744
XcmI	CCANNNNNNNNNTGG	1 cut(s)	632
XmaI	CCCGGG	1 cut(s)	167
XmiI	GTMKAC	1 cut(s)	1072
XspI	CTAG	5 cut(s)	282, 833, 1214, 1770, 1779
Zsp2I	ATGCAT	1 cut(s)	1169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.