Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004514

Physical Location & Seq

Genomic Coordinates Reverse (-)

3819490 .. 3821414

Gene Structure 1925 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g03940.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGAGATCGATCCTAATCAACCCTTCTCTTCTGTTTCTGGATGAACCAACTTCCGGCTTGGATTCTACAACTGCACTGCGAATTGGTCAGATGTTACAAGACATAGCAGAGGCTGGCAAGACTGTGGTGACAACAATCCACCAGCCATCAAGTCGACTCTTCCACAAATTTGACAAGTTGATCCTTCTTGGGAAGGGAAGCTTGCTCTACTTTGGAAAAGCATCAGAAGCAATGGTGTACTTCTCATCCATAGGATGTTCTCCACTTATTGCCATGAACCCAGCAGAGTTCTTGTTAGACCTTGCAAACGGAAATTTAAATGATATTTCAGTACCATCAGAATTAGAGGATAAGGTGCAAATGGGAAATTCAGAAGCAGATGATACAAGAAATGGAAAGCTATCTCCAGCAGTTGTGCATGAGTATCTTGTGGAGTCATACGAGGCACAAGTTGCAAACAAGGAGAAGAAGAAGCTTATGGCTCCCCTTCCCATCGATGAGGAACTGAAGTCAAAGATGTCATTTTCGAAGCGAGAATGGGGAACAAGCTGGTGGGAGCAATTTTCTATCTTGTTCTGGAGAGGAATTAAGGAAAGGAGGCATGACTACTTCAGCTGGTTGAGATTCACCCAAGTGCTCTCCACCGCGATGGTTTTGGGCTTGCTCTGGTGGCAGTCAGATGCTAACAATCCCAAAGGCCGGGAGGATCAGGCAGGGTTGCTTTTCTTCATTTCTGTTTTCTGGGGATTTTTACCCGTCTTCACAGCAATCTTCACATTTCCTCAAGAAAAAGCAATGCTGGTGAAGGAACGATCGGCTGACATGTACAGATTAAGTGCATATTTTGCTTCTAGGACTACTAGTGACCTCCCACTGGATCTAATGCTACCAGTTATGTTCCTCGTCGTTGTATATTTCATGGCTGGCTTAAGGCTGAGTGCTGAAACCTTCTTCCTAAGCATGCTTACGGTTTTCCTCTGCATTGTGGCAGCACAGGGACTTGGACTTGCTATCGGAGCTACATTGATGGACCTAAAGAAAGCCACAACTCTTGCATCAGTAATTGTGATGACATTCATGCTTGCTGGAGGGTTCTTTGTGAAAGAAGTTCCGGTGTTCATATCTTGGATTCGGTACATGTCTTTTGACTACCACACATACAGGATTCTTCTTAAGGTGCAGTACGAACACATCGCACCGACTGTTCACGGGTTGACCACAGACTGCGGTTTAACAGAAGTTGGTGCCCTTGTAGCCATGGTTTTTGGATACCGGCTCTTGGCATACCTTTCTTTGCGAAGAATGAAGCTTCAAGGTGGGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006833 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0010035 GO:0010148 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0042044 GO:0042221 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

442

Amino Acids

49.63

Weight (kDa)

7.06

Isoelectric Point (pI)

38.05

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06530 AT5G06530 AT5G06530 AT5G06530
fragaria_vesca	FvH4_6g21010 FvH4_6g21010 FvH4_6g21010
malus_domestica	MD12G1045900.v1.1 MD14G1044500.v1.1
prunus_persica	Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1 Prupe.7G089500_v2.0.a1
pyrus_communis	pycom12g03940 pycom14g03770
rosa_chinensis	RchiOBHm_Chr3g0477391
rosa_laevigata	RLG00000023726
rosa_multiflora	Rmu_ssc0000341.1_g000030
rosa_roxburghii	Rroxscaffold_1G00008820 Rroxscaffold_3G00241160 Rroxscaffold_4G00299920 Rroxscaffold_6G00404230 Rroxscaffold_6G00404700
rosa_rugosa	Rorug03G0159900 Rorug03G0160000 Rorug03G0160100
rosa_samantha	Rh3AG210600 Rh3BG243800 Rh3CG237700 Rh3DG237400
rosa_wichuraiana	Rw3G019080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1244
AccI	GTMKAC	1 cut(s)	154
AccII	CGCG	1 cut(s)	647
AciI	CCGC	2 cut(s)	645, 1227
AclWI	GGATC	4 cut(s)	4, 175, 714, 885
AcsI	RAATTY	3 cut(s)	167, 313, 367
AcuI	CTGAAG	2 cut(s)	527, 595
AfaI	GTAC	5 cut(s)	239, 333, 827, 1136, 1184
AfiI	CCNNNNNNNGG	4 cut(s)	53, 699, 874, 1279
AflII	CTTAAG	2 cut(s)	928, 1172
AflIII	ACRYGT	2 cut(s)	822, 1137
AgsI	TTSAA	1 cut(s)	1313
AhlI	ACTAGT	1 cut(s)	860
AleI	CACNNNNGTG	1 cut(s)	632
AluBI	AGCT	7 cut(s)	201, 400, 475, 549, 615, 1019, 1309
AluI	AGCT	7 cut(s)	201, 400, 475, 549, 615, 1019, 1309
Alw21I	GWGCWC	1 cut(s)	639
AlwI	GGATC	4 cut(s)	4, 175, 714, 885
AlwNI	CAGNNNCTG	1 cut(s)	113
AoxI	GGCC	1 cut(s)	697
ApeKI	GCWGC	1 cut(s)	989
ApoI	RAATTY	3 cut(s)	167, 313, 367
AspS9I	GGNCC	1 cut(s)	1030
AsuC2I	CCSGG	1 cut(s)	701
AsuHPI	GGTGA	3 cut(s)	139, 619, 814
AsuII	TTCGAA	1 cut(s)	527
AvaII	GGWCC	1 cut(s)	1030
BaeGI	GKGCMC	1 cut(s)	1249
BanI	GGYRCC	1 cut(s)	1244
BbsI	GAAGAC	1 cut(s)	751
Bbv12I	GWGCWC	1 cut(s)	639
BbvI	GCAGC	1 cut(s)	1001
BccI	CCATC	5 cut(s)	154, 343, 500, 643, 1021
BciVI	GTATCC	1 cut(s)	1262
BcnI	CCSGG	1 cut(s)	701
BcuI	ACTAGT	1 cut(s)	860
BfaI	CTAG	2 cut(s)	852, 861
BfrI	CTTAAG	2 cut(s)	928, 1172
BfuI	GTATCC	1 cut(s)	1262
BisI	GCNGC	1 cut(s)	990
BlsI	GCNGC	1 cut(s)	991
Bme1390I	CCNGG	1 cut(s)	701
Bme18I	GGWCC	1 cut(s)	1030
BmgT120I	GGNCC	1 cut(s)	1030
BmiI	GGNNCC	2 cut(s)	483, 1246
BmrFI	CCNGG	1 cut(s)	701
BmsI	GCATC	3 cut(s)	230, 670, 1064
BpiI	GAAGAC	1 cut(s)	751
BpmI	CTGGAG	3 cut(s)	390, 598, 1107
Bpu10I	CCTNAGC	1 cut(s)	956
Bpu14I	TTCGAA	1 cut(s)	527
BpuEI	CTTGAG	1 cut(s)	768
BpuMI	CCSGG	1 cut(s)	701
Bsa29I	ATCGAT	2 cut(s)	8, 495
BsaBI	GATNNNNATC	1 cut(s)	14
BsaJI	CCNNGG	1 cut(s)	1257
BsaWI	WCCGGW	1 cut(s)	1111
Bsc4I	CCNNNNNNNGG	4 cut(s)	53, 699, 874, 1279
Bse118I	RCCGGY	1 cut(s)	1272
Bse1I	ACTGG	2 cut(s)	879, 890
Bse3DI	GCAATG	2 cut(s)	237, 801
Bse8I	GATNNNNATC	1 cut(s)	14
BseCI	ATCGAT	2 cut(s)	8, 495
BseDI	CCNNGG	1 cut(s)	1257
BseGI	GGATG	3 cut(s)	46, 245, 260
BseJI	GATNNNNATC	1 cut(s)	14
BseLI	CCNNNNNNNGG	4 cut(s)	53, 699, 874, 1279
BseMI	GCAATG	2 cut(s)	237, 801
BseMII	CTCAG	1 cut(s)	926
BseNI	ACTGG	2 cut(s)	879, 890
BseSI	GKGCMC	1 cut(s)	1249
BseXI	GCAGC	1 cut(s)	1001
BseYI	CCCAGC	1 cut(s)	280
BsgI	GTGCAG	2 cut(s)	57, 1199
Bsh1236I	CGCG	1 cut(s)	647
Bsh1285I	CGRYCG	1 cut(s)	815
BshFI	GGCC	1 cut(s)	699
BshNI	GGYRCC	1 cut(s)	1244
BshVI	ATCGAT	2 cut(s)	8, 495
BsiEI	CGRYCG	1 cut(s)	815
BsiHKAI	GWGCWC	1 cut(s)	639
BsiSI	CCGG	4 cut(s)	54, 700, 1112, 1273
BslFI	GGGAC	1 cut(s)	1011
BslI	CCNNNNNNNGG	4 cut(s)	53, 699, 874, 1279
BsmFI	GGGAC	1 cut(s)	1011
BsnI	GGCC	1 cut(s)	699
Bsp119I	TTCGAA	1 cut(s)	527
Bsp1286I	GDGCHC	2 cut(s)	639, 1249
Bsp1407I	TGTACA	1 cut(s)	825
Bsp143I	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
Bsp19I	CCATGG	1 cut(s)	1257
BspACI	CCGC	2 cut(s)	645, 1227
BspANI	GGCC	1 cut(s)	699
BspCNI	CTCAG	1 cut(s)	927
BspDI	ATCGAT	2 cut(s)	8, 495
BspFNI	CGCG	1 cut(s)	647
BspLI	GGNNCC	2 cut(s)	483, 1246
BspPI	GGATC	4 cut(s)	4, 175, 714, 885
BspT104I	TTCGAA	1 cut(s)	527
BspT107I	GGYRCC	1 cut(s)	1244
BspTI	CTTAAG	2 cut(s)	928, 1172
BsrDI	GCAATG	2 cut(s)	237, 801
BsrFI	RCCGGY	1 cut(s)	1272
BsrGI	TGTACA	1 cut(s)	825
BsrI	ACTGG	2 cut(s)	879, 890
BssAI	RCCGGY	1 cut(s)	1272
BssECI	CCNNGG	1 cut(s)	1257
BssMI	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
BssT1I	CCWWGG	1 cut(s)	1257
Bst4CI	ACNGT	3 cut(s)	124, 970, 1204
Bst6I	CTCTTC	2 cut(s)	33, 164
BstAFI	CTTAAG	2 cut(s)	928, 1172
BstAPI	GCANNNNNTGC	2 cut(s)	452, 845
BstAUI	TGTACA	1 cut(s)	825
BstBI	TTCGAA	1 cut(s)	527
BstC8I	GCNNGC	6 cut(s)	115, 203, 662, 925, 962, 1083
BstDEI	CTNAG	2 cut(s)	935, 956
BstDSI	CCRYGG	1 cut(s)	1257
BstF5I	GGATG	3 cut(s)	46, 245, 260
BstFNI	CGCG	1 cut(s)	647
BstKTI	GATC	6 cut(s)	8, 12, 183, 709, 815, 880
BstMBI	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
BstMCI	CGRYCG	1 cut(s)	815
BstMWI	GCNNNNNNNGC	6 cut(s)	227, 452, 670, 845, 1016, 1253
BstNSI	RCATGY	3 cut(s)	826, 964, 1141
BstSCI	CCNGG	1 cut(s)	699
BstSLI	GKGCMC	1 cut(s)	1249
BstUI	CGCG	1 cut(s)	647
BstV1I	GCAGC	1 cut(s)	1001
BstV2I	GAAGAC	1 cut(s)	751
BstX2I	RGATCY	1 cut(s)	877
BstXI	CCANNNNNNTGG	1 cut(s)	649
BstYI	RGATCY	1 cut(s)	877
Bsu15I	ATCGAT	2 cut(s)	8, 495
BsuI	GTATCC	1 cut(s)	1262
BsuRI	GGCC	1 cut(s)	699
BsuTUI	ATCGAT	2 cut(s)	8, 495
BtgI	CCRYGG	1 cut(s)	1257
BtgZI	GCGATG	2 cut(s)	662, 1177
BtsCI	GGATG	3 cut(s)	46, 245, 260
BtsI	GCAGTG	1 cut(s)	74
BtsIMutI	CAGTG	2 cut(s)	74, 872
Cac8I	GCNNGC	6 cut(s)	115, 203, 662, 925, 962, 1083
CaiI	CAGNNNCTG	1 cut(s)	113
Cfr10I	RCCGGY	1 cut(s)	1272
Cfr13I	GGNCC	1 cut(s)	1030
ClaI	ATCGAT	2 cut(s)	8, 495
Csp6I	GTAC	5 cut(s)	238, 332, 826, 1135, 1183
CspCI	CAANNNNNGTGG	2 cut(s)	1033, 1068
CviAII	CATG	9 cut(s)	274, 419, 602, 823, 919, 961, 1078, 1138, 1258
CviQI	GTAC	5 cut(s)	238, 332, 826, 1135, 1183
DdeI	CTNAG	2 cut(s)	935, 956
DpnI	GATC	6 cut(s)	7, 11, 182, 708, 814, 879
DpnII	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
DraI	TTTAAA	1 cut(s)	318
Eam1104I	CTCTTC	2 cut(s)	33, 164
EarI	CTCTTC	2 cut(s)	33, 164
Eco130I	CCWWGG	1 cut(s)	1257
Eco47I	GGWCC	1 cut(s)	1030
Eco57I	CTGAAG	2 cut(s)	527, 595
EcoT14I	CCWWGG	1 cut(s)	1257
ErhI	CCWWGG	1 cut(s)	1257
FaeI	CATG	9 cut(s)	277, 422, 605, 826, 922, 964, 1081, 1141, 1261
FalI	AAGNNNNNCTT	2 cut(s)	185, 217
FaqI	GGGAC	1 cut(s)	1011
FatI	CATG	9 cut(s)	273, 418, 601, 822, 918, 960, 1077, 1137, 1257
FblI	GTMKAC	1 cut(s)	154
Fnu4HI	GCNGC	1 cut(s)	990
FokI	GGATG	3 cut(s)	53, 232, 267
Fsp4HI	GCNGC	1 cut(s)	990
FspBI	CTAG	2 cut(s)	852, 861
GluI	GCNGC	1 cut(s)	990
GsaI	CCCAGC	1 cut(s)	284
GsuI	CTGGAG	3 cut(s)	390, 598, 1107
HaeIII	GGCC	1 cut(s)	699
HapII	CCGG	4 cut(s)	54, 700, 1112, 1273
Hin1II	CATG	9 cut(s)	277, 422, 605, 826, 922, 964, 1081, 1141, 1261
HincII	GTYRAC	2 cut(s)	155, 1215
HindII	GTYRAC	2 cut(s)	155, 1215
HindIII	AAGCTT	3 cut(s)	199, 473, 1307
HinfI	GANTC	6 cut(s)	62, 156, 434, 624, 1129, 1165
HpaII	CCGG	4 cut(s)	54, 700, 1112, 1273
HphI	GGTGA	3 cut(s)	139, 619, 814
Hpy166II	GTNNAC	4 cut(s)	155, 238, 1207, 1215
Hpy188I	TCNGA	6 cut(s)	90, 226, 340, 373, 679, 1016
Hpy188III	TCNNGA	3 cut(s)	38, 577, 785
Hpy8I	GTNNAC	4 cut(s)	155, 238, 1207, 1215
Hpy99I	CGWCG	1 cut(s)	908
HpyAV	CCTTC	6 cut(s)	33, 187, 194, 497, 799, 958
HpyCH4III	ACNGT	3 cut(s)	124, 970, 1204
HpyCH4V	TGCA	9 cut(s)	74, 305, 358, 418, 455, 839, 981, 1055, 1180
HpyF10VI	GCNNNNNNNGC	6 cut(s)	227, 452, 670, 845, 1016, 1253
HpyF3I	CTNAG	2 cut(s)	935, 956
Hsp92II	CATG	9 cut(s)	277, 422, 605, 826, 922, 964, 1081, 1141, 1261
Kzo9I	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
LmnI	GCTCC	3 cut(s)	487, 556, 1016
Lsp1109I	GCAGC	1 cut(s)	1001
LweI	GCATC	3 cut(s)	230, 670, 1064
MaeI	CTAG	2 cut(s)	852, 861
MaeIII	GTNAC	3 cut(s)	93, 127, 863
MalI	GATC	6 cut(s)	7, 11, 182, 708, 814, 879
MboI	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
MflI	RGATCY	1 cut(s)	877
MhlI	GDGCHC	2 cut(s)	639, 1249
MluCI	AATT	8 cut(s)	81, 167, 313, 341, 367, 560, 585, 1062
MlyI	GAGTC	2 cut(s)	150, 443
MseI	TTAA	6 cut(s)	317, 588, 833, 929, 1173, 1232
MslI	CAYNNNNRTG	2 cut(s)	632, 647
MspA1I	CMGCKG	1 cut(s)	615
MspCI	CTTAAG	2 cut(s)	928, 1172
MspI	CCGG	4 cut(s)	54, 700, 1112, 1273
MspR9I	CCNGG	1 cut(s)	701
MvnI	CGCG	1 cut(s)	647
MwoI	GCNNNNNNNGC	6 cut(s)	227, 452, 670, 845, 1016, 1253
NciI	CCSGG	1 cut(s)	701
NcoI	CCATGG	1 cut(s)	1257
NdeII	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
NlaIII	CATG	9 cut(s)	277, 422, 605, 826, 922, 964, 1081, 1141, 1261
NlaIV	GGNNCC	2 cut(s)	483, 1246
NmuCI	GTSAC	2 cut(s)	127, 863
NspI	RCATGY	3 cut(s)	826, 964, 1141
NspV	TTCGAA	1 cut(s)	527
OliI	CACNNNNGTG	1 cut(s)	632
PaeI	GCATGC	1 cut(s)	964
PciI	ACATGT	2 cut(s)	822, 1137
PfeI	GAWTC	4 cut(s)	62, 624, 1129, 1165
PkrI	GCNGC	1 cut(s)	991
Ple19I	CGATCG	1 cut(s)	815
PleI	GAGTC	2 cut(s)	150, 442
PpsI	GAGTC	2 cut(s)	150, 442
PscI	ACATGT	2 cut(s)	822, 1137
PspFI	CCCAGC	1 cut(s)	280
PspN4I	GGNNCC	2 cut(s)	483, 1246
PspPI	GGNCC	1 cut(s)	1030
PstNI	CAGNNNCTG	1 cut(s)	113
PsuI	RGATCY	1 cut(s)	877
PvuI	CGATCG	1 cut(s)	815
PvuII	CAGCTG	1 cut(s)	615
RsaI	GTAC	5 cut(s)	239, 333, 827, 1136, 1184
RsaNI	GTAC	5 cut(s)	238, 332, 826, 1135, 1183
RseI	CAYNNNNRTG	2 cut(s)	632, 647
SalI	GTCGAC	1 cut(s)	153
SaqAI	TTAA	6 cut(s)	317, 588, 833, 929, 1173, 1232
SatI	GCNGC	1 cut(s)	990
Sau3AI	GATC	6 cut(s)	5, 9, 180, 706, 812, 877
Sau96I	GGNCC	1 cut(s)	1030
SchI	GAGTC	2 cut(s)	150, 443
ScrFI	CCNGG	1 cut(s)	701
SduI	GDGCHC	2 cut(s)	639, 1249
SfaNI	GCATC	3 cut(s)	230, 670, 1064
SfuI	TTCGAA	1 cut(s)	527
SinI	GGWCC	1 cut(s)	1030
SmiI	ATTTAAAT	1 cut(s)	318
SmiMI	CAYNNNNRTG	2 cut(s)	632, 647
SmlI	CTYRAG	3 cut(s)	783, 928, 1172
SmoI	CTYRAG	3 cut(s)	783, 928, 1172
SpeI	ACTAGT	1 cut(s)	860
SphI	GCATGC	1 cut(s)	964
Sse9I	AATT	8 cut(s)	81, 167, 313, 341, 367, 560, 585, 1062
SsiI	CCGC	2 cut(s)	645, 1227
SspMI	CTAG	2 cut(s)	852, 861
StyD4I	CCNGG	1 cut(s)	699
StyI	CCWWGG	1 cut(s)	1257
SwaI	ATTTAAAT	1 cut(s)	318
TaaI	ACNGT	3 cut(s)	124, 970, 1204
TaqI	TCGA	4 cut(s)	8, 154, 495, 527
TasI	AATT	8 cut(s)	81, 167, 313, 341, 367, 560, 585, 1062
TatI	WGTACW	2 cut(s)	237, 825
TfiI	GAWTC	4 cut(s)	62, 624, 1129, 1165
Tru1I	TTAA	6 cut(s)	317, 588, 833, 929, 1173, 1232
Tru9I	TTAA	6 cut(s)	317, 588, 833, 929, 1173, 1232
TscAI	CASTG	2 cut(s)	81, 879
TseFI	GTSAC	2 cut(s)	127, 863
TseI	GCWGC	1 cut(s)	989
Tsp45I	GTSAC	2 cut(s)	127, 863
TspDTI	ATGAA	7 cut(s)	57, 290, 718, 907, 1066, 1108, 1319
TspGWI	ACGGA	1 cut(s)	324
TspRI	CASTG	2 cut(s)	81, 879
Vha464I	CTTAAG	2 cut(s)	928, 1172
VpaK11BI	GGWCC	1 cut(s)	1030
XapI	RAATTY	3 cut(s)	167, 313, 367
XceI	RCATGY	3 cut(s)	826, 964, 1141
XmiI	GTMKAC	1 cut(s)	154
XspI	CTAG	2 cut(s)	852, 861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.