MD12G1053200.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011155
Physical Location & Seq
Forward (+)
5941239 .. 5946215
4977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1053200.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAAGAGAAACAAGTAGAAGAAGCAGGTGGGGAGGAGGTGGCTTGGAGCAGAGAAACGCTTCCCAAAGTTCTGAAAATAGTTAGCACAAGACTGCCTCAGAGGGACCTCATCTCTCTCTTGCTAGTCAGCCCATGGCTCAACGCAACTCTCATCTCCTACCCTTCCCTTTGGCTGCTTCTGGAACTTCGAGAAATGAATAATGCTGGGAATCGCCTTGTGGATGCTCTTTCATTGGCGAGGTATCAGCATGTGAAGCAGATAAACCTTGAATTTTCACAAGACATTGAAGACAAGTATCTTGAACTCGTAAAGAGTAAGTGTCATGATTCTCTTCAAAACCTGGAGGTTTTGAATCTTAATGGCTGCCAGAAGATCTCTGATAAGGGAATTGAAGCTATAACCAGTTCTTGCCCCAATATGAAGGAGTTTTCAATCTATTGGAATGTGAGGGCAACAGATATAGGTATAATGCACCTGGTGAAAAACTGCAAGCATGTAGTTGATCTGAATTTAAGTGGCTGTAAGAATCTTTCAGACAAAAGCTTGCAATTAGTTGCTGAGAATTATCCAGAATTACAGTTGTTGAACCTGACCAGGTGTGTCAAGTTAACAGATTCTGGCTTGCAGCAGATATTGCACAGTTGTCCTTGTCTCCATAGTCTAAATTTGTATGCCCTGTCAAGCTTCACTGATGAAGCTTACAAGAAAATATCACTTCTTTCCCATCTTCAATTCTTGGATTTATGCGGTGCTCAGAATCTATCGGATGAAGGGGTGTCTTGTATAGCTAGATGCAAGAGCCTTTTGTCTCTCAATTTGACATGGTGTGTACGGGTCACTGATTTGGGGGTCATAGCCGTTGCGCAGGGTTGTACGTCGCTTGAATTTCTCAGCTTATTTGGGATAGTTGGGGTGACTGATAAATGCTTGGAGCCCCTTTCACTGACCTGCTCAAACACACTTACGACTCTTGATGTTAATGGATGTATTGGAATTAAGAAACGGAGCCGTGATGAATTGCTTCAAATGTTTCCAAAGTTGCGGTGCTTCAAAGTGCACAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

356

Amino Acids

39.73

Weight (kDa)

6.26

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 151 - 333 4e-15 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58530
fragaria_vesca FvH4_6g21620 FvH4_6g21620
malus_domestica MD12G1053200.v1.1 MD14G1051600.v1.1
prunus_persica Prupe.7G084400_v2.0.a1
pyrus_communis pycom12g04660 pycom14g04290 pycom14g04300
rosa_chinensis RchiOBHm_Chr3g0478871
rosa_laevigata RLG00000023619
rosa_multiflora Rmu_sc0004831.1_g000004 Rmu_sc0005751.1_g000013
rosa_roxburghii Rroxscaffold_6G00403010
rosa_rugosa Rorug03G0168800
rosa_samantha Rh3AG220400 Rh3BG252300 Rh3CG249100 Rh3DG245300
rosa_wichuraiana Rw3G019820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 867
Acc36I ACCTGC 2 cut(s) 17, 959
AciI CCGC 2 cut(s) 750, 1045
AcsI RAATTY 4 cut(s) 272, 511, 667, 887
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 2 cut(s) 834, 877
AjnI CCWGG 3 cut(s) 342, 477, 596
AluBI AGCT 7 cut(s) 398, 546, 687, 701, 791, 897, 1065
AluI AGCT 7 cut(s) 398, 546, 687, 701, 791, 897, 1065
Alw21I GWGCWC 2 cut(s) 757, 1062
Alw26I GTCTC 2 cut(s) 659, 816
Alw44I GTGCAC 1 cut(s) 1058
AlwNI CAGNNNCTG 1 cut(s) 620
ApaLI GTGCAC 1 cut(s) 1058
ApeKI GCWGC 3 cut(s) 175, 366, 628
ApoI RAATTY 4 cut(s) 272, 511, 667, 887
Asp700I GAANNNNTTC 2 cut(s) 60, 1023
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 493, 928
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 1062
BanII GRGCYC 1 cut(s) 939
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 757, 1062
BbvI GCAGC 3 cut(s) 162, 353, 640
BccI CCATC 1 cut(s) 735
BceAI ACGGC 2 cut(s) 845, 996
BciT130I CCWGG 3 cut(s) 344, 479, 598
BcoDI GTCTC 2 cut(s) 659, 816
BfaI CTAG 2 cut(s) 125, 792
BfuAI ACCTGC 2 cut(s) 17, 959
BglII AGATCT 1 cut(s) 375
BisI GCNGC 3 cut(s) 176, 367, 629
BlsI GCNGC 3 cut(s) 177, 368, 630
Bme1390I CCNGG 3 cut(s) 344, 479, 598
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 3 cut(s) 107, 936, 1010
BmrFI CCNGG 3 cut(s) 344, 479, 598
BmsI GCATC 2 cut(s) 214, 785
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 405
BseBI CCWGG 3 cut(s) 344, 479, 598
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 3 cut(s) 229, 775, 992
BseMII CTCAG 4 cut(s) 113, 552, 770, 907
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 1062
BseXI GCAGC 3 cut(s) 162, 353, 640
BseYI CCCAGC 1 cut(s) 206
BsiHKAI GWGCWC 2 cut(s) 757, 1062
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 2 cut(s) 659, 816
BsmFI GGGAC 1 cut(s) 119
Bsp1286I GDGCHC 3 cut(s) 757, 939, 1062
Bsp143I GATC 2 cut(s) 375, 505
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 2 cut(s) 750, 1045
BspCNI CTCAG 4 cut(s) 112, 553, 769, 906
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 107, 936, 1010
BspMI ACCTGC 2 cut(s) 17, 959
BsrI ACTGG 1 cut(s) 405
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 2 cut(s) 375, 505
BssT1I CCWWGG 1 cut(s) 134
Bst2UI CCWGG 3 cut(s) 344, 479, 598
Bst4CI ACNGT 2 cut(s) 582, 644
Bst6I CTCTTC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 637
BstC8I GCNNGC 3 cut(s) 494, 548, 626
BstDEI CTNAG 4 cut(s) 99, 561, 756, 893
BstDSI CCRYGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 229, 775, 992
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 2 cut(s) 378, 508
BstMAI GTCTC 2 cut(s) 659, 816
BstMBI GATC 2 cut(s) 375, 505
BstMWI GCNNNNNNNGC 2 cut(s) 256, 637
BstNI CCWGG 3 cut(s) 344, 479, 598
BstNSI RCATGY 2 cut(s) 254, 500
BstSCI CCNGG 3 cut(s) 342, 477, 596
BstSLI GKGCMC 1 cut(s) 1062
BstV1I GCAGC 3 cut(s) 162, 353, 640
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 375
BstYI RGATCY 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 134
BtsCI GGATG 3 cut(s) 229, 775, 992
BtsIMutI CAGTG 3 cut(s) 690, 840, 944
BveI ACCTGC 2 cut(s) 17, 959
Cac8I GCNNGC 3 cut(s) 494, 548, 626
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 868
Cfr13I GGNCC 1 cut(s) 106
CsiI ACCWGGT 2 cut(s) 477, 596
Csp6I GTAC 2 cut(s) 833, 876
CviAII CATG 5 cut(s) 135, 251, 326, 497, 825
CviQI GTAC 2 cut(s) 833, 876
DdeI CTNAG 4 cut(s) 99, 561, 756, 893
DpnI GATC 2 cut(s) 377, 507
DpnII GATC 2 cut(s) 375, 505
DraIII CACNNNGTG 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 939
Eco47I GGWCC 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 3 cut(s) 342, 477, 596
EcoT14I CCWWGG 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 939
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 5 cut(s) 138, 254, 329, 500, 828
FaqI GGGAC 1 cut(s) 119
FatI CATG 5 cut(s) 134, 250, 325, 496, 824
Fnu4HI GCNGC 3 cut(s) 176, 367, 629
FokI GGATG 3 cut(s) 236, 782, 999
FriOI GRGCYC 1 cut(s) 939
Fsp4HI GCNGC 3 cut(s) 176, 367, 629
FspBI CTAG 2 cut(s) 125, 792
FspI TGCGCA 1 cut(s) 867
GlaI GCGC 1 cut(s) 867
GluI GCNGC 3 cut(s) 176, 367, 629
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 365
HhaI GCGC 1 cut(s) 868
Hin1II CATG 5 cut(s) 138, 254, 329, 500, 828
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 3 cut(s) 544, 685, 699
HinfI GANTC 7 cut(s) 211, 329, 355, 529, 617, 760, 970
HpaI GTTAAC 1 cut(s) 612
HphI GGTGA 2 cut(s) 493, 928
Hpy166II GTNNAC 3 cut(s) 612, 833, 1060
Hpy188I TCNGA 7 cut(s) 75, 102, 382, 510, 538, 759, 769
Hpy188III TCNNGA 6 cut(s) 182, 191, 302, 326, 572, 974
Hpy8I GTNNAC 3 cut(s) 612, 833, 1060
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 3 cut(s) 174, 418, 767
HpyCH4III ACNGT 2 cut(s) 582, 644
HpyCH4IV ACGT 1 cut(s) 878
HpyCH4V TGCA 7 cut(s) 475, 492, 550, 628, 640, 798, 1060
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 637
HpyF3I CTNAG 4 cut(s) 99, 561, 756, 893
HpySE526I ACGT 1 cut(s) 878
Hsp92II CATG 5 cut(s) 138, 254, 329, 500, 828
HspAI GCGC 1 cut(s) 866
KspAI GTTAAC 1 cut(s) 612
Kzo9I GATC 2 cut(s) 375, 505
LmnI GCTCC 3 cut(s) 48, 934, 1008
Lsp1109I GCAGC 3 cut(s) 162, 353, 640
LweI GCATC 2 cut(s) 214, 785
MabI ACCWGGT 2 cut(s) 477, 596
MaeI CTAG 2 cut(s) 125, 792
MaeII ACGT 1 cut(s) 878
MaeIII GTNAC 2 cut(s) 838, 916
MalI GATC 2 cut(s) 377, 507
MboI GATC 2 cut(s) 375, 505
MboII GAAGA 6 cut(s) 17, 32, 302, 326, 385, 722
MflI RGATCY 1 cut(s) 375
MhlI GDGCHC 3 cut(s) 757, 939, 1062
MlyI GAGTC 1 cut(s) 964
MnlI CCTC 8 cut(s) 28, 31, 96, 108, 119, 234, 340, 444
MroXI GAANNNNTTC 2 cut(s) 60, 1023
MseI TTAA 5 cut(s) 360, 515, 611, 981, 999
MspR9I CCNGG 3 cut(s) 344, 479, 598
MvaI CCWGG 3 cut(s) 344, 479, 598
MwoI GCNNNNNNNGC 2 cut(s) 256, 637
NcoI CCATGG 1 cut(s) 134
NdeII GATC 2 cut(s) 375, 505
NlaIII CATG 5 cut(s) 138, 254, 329, 500, 828
NlaIV GGNNCC 3 cut(s) 107, 936, 1010
NmuCI GTSAC 2 cut(s) 838, 916
NsbI TGCGCA 1 cut(s) 867
NspI RCATGY 2 cut(s) 254, 500
PagI TCATGA 1 cut(s) 325
PaqCI CACCTGC 1 cut(s) 17
PdmI GAANNNNTTC 2 cut(s) 60, 1023
PfeI GAWTC 6 cut(s) 211, 329, 355, 529, 617, 760
PkrI GCNGC 3 cut(s) 177, 368, 630
PleI GAGTC 1 cut(s) 964
PpsI GAGTC 1 cut(s) 964
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 3 cut(s) 342, 477, 596
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 3 cut(s) 342, 477, 596
PspN4I GGNNCC 3 cut(s) 107, 936, 1010
PspPI GGNCC 1 cut(s) 106
PspPPI RGGWCCY 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 375
RsaI GTAC 2 cut(s) 834, 877
RsaNI GTAC 2 cut(s) 833, 876
SaqAI TTAA 5 cut(s) 360, 515, 611, 981, 999
SatI GCNGC 3 cut(s) 176, 367, 629
Sau3AI GATC 2 cut(s) 375, 505
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 1 cut(s) 964
ScrFI CCNGG 3 cut(s) 344, 479, 598
SduI GDGCHC 3 cut(s) 757, 939, 1062
SexAI ACCWGGT 2 cut(s) 477, 596
SfaNI GCATC 2 cut(s) 214, 785
SinI GGWCC 1 cut(s) 106
SsiI CCGC 2 cut(s) 750, 1045
SspMI CTAG 2 cut(s) 125, 792
StyD4I CCNGG 3 cut(s) 342, 477, 596
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 2 cut(s) 582, 644
TaiI ACGT 1 cut(s) 881
TaqI TCGA 1 cut(s) 190
TfiI GAWTC 6 cut(s) 211, 329, 355, 529, 617, 760
Tru1I TTAA 5 cut(s) 360, 515, 611, 981, 999
Tru9I TTAA 5 cut(s) 360, 515, 611, 981, 999
TscAI CASTG 3 cut(s) 697, 847, 951
TseFI GTSAC 2 cut(s) 838, 916
TseI GCWGC 3 cut(s) 175, 366, 628
Tsp45I GTSAC 2 cut(s) 838, 916
TspDTI ATGAA 6 cut(s) 212, 222, 437, 711, 786, 1032
TspGWI ACGGA 1 cut(s) 1021
TspRI CASTG 3 cut(s) 697, 847, 951
VneI GTGCAC 1 cut(s) 1058
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 4 cut(s) 272, 511, 667, 887
XceI RCATGY 2 cut(s) 254, 500
XmnI GAANNNNTTC 2 cut(s) 60, 1023
XspI CTAG 2 cut(s) 125, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.