MD14G1051600.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011155
Physical Location & Seq
Forward (+)
4941184 .. 4945996
4813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1051600.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGGAGGAGAAACAAGTAGAAGTAGGTGAGGAGGAAGTAGCTTGGAGCAGAGAAATCATTCCCAAAGTTCTGAGAATAGTGAGCACAAGACTGCCGCAGAGAGACCTCATCTTTCTCTTGCTAGTTAGCCCATGGCTCAACGCAACTCTCGTCTCCTGCCCTTCCATTTGGCTGCATCTGGATTTTCGAGAGATGAATAATGCTGGGAATCGCCTTGTGGATGCTCTTTCATTGGCAAGATATCGGCATGTGAAGCATATAAACCTTGAATTTGCACAAGACATTGAAGACAAGCATCTTGAACTTGTAAAGAGCAAGTGTCAAGATTCTCTTCAAGATCTGGAGGTTATGAATCTTAATGGCTGCCAGAAGATATCTGATAAGGGAATTGAAGCTATAACCAGTGCTTGCCCCAGTTTGAAGGAATTTTTGAGCTATTGGAATGTGAGGGTAACAGATATAGGTTTAGCGCACCTGGTGAAGAACTGCAAACATATAGTTGATCTGAACGTAAGTGGCTGTAAGAACCTTTCAGACAAAAGCTTGCAATTAATTGCTCAGAATTATCCAGAATTACAGTTGTTGAACCTGACAAGGTGTGTCAAGTTAACAGATTCTGGCCTGCAGCAGATATTGCACGGTTGTCCTAGTCTCCATAGTCTAAATTTGTACGCCCTGTCAAGCTTCACTGATGAAGCTTACAAGAAAATATCACTTCTTTCCCATCTTAAATTCTTGGATTTATGTGGCGCCCAGAATCTGTCAGATGAAGGGGTGGGTTGTATAGCTAGATGCAAGGGCCTTCTTTCTCTCAATTTGACATGGTGTGTACGGGTCACTGACGTGGGGGTCATAGCCGTTGCGCAGGCTTGCACATTTCTTGAATTTCTCAGCTTGTTTGGGATAGTTGGGGTGACCGATAAATGCTTGGAGGCCCTTTCACTGACCTGCTCAAACACACTCACAACTCTTGATGTTAATGGATGTATTGGAATTAAGAAACGGAGCCGTGATGAATTGCTTCAGATGTTTCCAAAGTTACGATGCTTCAAAGTGCACAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

355

Amino Acids

39.62

Weight (kDa)

6.78

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 111 - 180 1.5e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 150 - 284 1.7e-13 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58530
fragaria_vesca FvH4_6g21620 FvH4_6g21620
malus_domestica MD12G1053200.v1.1 MD14G1051600.v1.1
prunus_persica Prupe.7G084400_v2.0.a1
pyrus_communis pycom12g04660 pycom14g04290 pycom14g04300
rosa_chinensis RchiOBHm_Chr3g0478871
rosa_laevigata RLG00000023619
rosa_multiflora Rmu_sc0004831.1_g000004 Rmu_sc0005751.1_g000013
rosa_roxburghii Rroxscaffold_6G00403010
rosa_rugosa Rorug03G0168800
rosa_samantha Rh3AG220400 Rh3BG252300 Rh3CG249100 Rh3DG245300
rosa_wichuraiana Rw3G019820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 848
Acc16I TGCGCA 1 cut(s) 864
Acc36I ACCTGC 1 cut(s) 956
AccB1I GGYRCC 1 cut(s) 749
AciI CCGC 1 cut(s) 95
AcsI RAATTY 5 cut(s) 269, 425, 664, 731, 884
AcuI CTGAAG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 750
AdeI CACNNNGTG 1 cut(s) 478
AfaI GTAC 2 cut(s) 671, 831
AgsI TTSAA 9 cut(s) 269, 287, 302, 335, 392, 421, 586, 884, 1051
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 474
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 9 cut(s) 41, 395, 435, 543, 684, 698, 788, 894, 1062
AluI AGCT 9 cut(s) 41, 395, 435, 543, 684, 698, 788, 894, 1062
Alw21I GWGCWC 2 cut(s) 86, 1059
Alw26I GTCTC 3 cut(s) 96, 157, 656
Alw44I GTGCAC 1 cut(s) 1055
AlwNI CAGNNNCTG 2 cut(s) 617, 760
AoxI GGCC 3 cut(s) 619, 799, 933
ApaLI GTGCAC 1 cut(s) 1055
ApeKI GCWGC 3 cut(s) 172, 363, 625
ApoI RAATTY 5 cut(s) 269, 425, 664, 731, 884
AseI ATTAAT 1 cut(s) 551
Asp700I GAANNNNTTC 2 cut(s) 57, 1020
AspLEI GCGC 3 cut(s) 472, 752, 865
AspS9I GGNCC 2 cut(s) 799, 934
AsuHPI GGTGA 3 cut(s) 38, 490, 925
BaeGI GKGCMC 1 cut(s) 1059
BanI GGYRCC 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 86, 1059
BbvI GCAGC 3 cut(s) 159, 350, 637
BccI CCATC 1 cut(s) 732
BceAI ACGGC 2 cut(s) 842, 993
BciT130I CCWGG 1 cut(s) 476
BcoDI GTCTC 3 cut(s) 96, 157, 656
BfaI CTAG 3 cut(s) 122, 648, 789
BfmI CTRYAG 1 cut(s) 623
BfoI RGCGCY 1 cut(s) 753
BfuAI ACCTGC 1 cut(s) 956
BglII AGATCT 1 cut(s) 337
BisI GCNGC 4 cut(s) 95, 173, 364, 626
BlsI GCNGC 4 cut(s) 96, 174, 365, 627
Bme1390I CCNGG 1 cut(s) 476
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 2 cut(s) 799, 934
BmiI GGNNCC 2 cut(s) 751, 1007
BmrFI CCNGG 1 cut(s) 476
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 5 cut(s) 184, 211, 304, 782, 1034
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 750
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 402, 414
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 131
BseGI GGATG 2 cut(s) 226, 989
BseMII CTCAG 3 cut(s) 62, 572, 904
BseNI ACTGG 2 cut(s) 402, 414
BseRI GAGGAG 2 cut(s) 20, 44
BseSI GKGCMC 1 cut(s) 1059
BseXI GCAGC 3 cut(s) 159, 350, 637
BseYI CCCAGC 1 cut(s) 203
BshFI GGCC 3 cut(s) 621, 801, 935
BshNI GGYRCC 1 cut(s) 749
BsiHKAI GWGCWC 2 cut(s) 86, 1059
BsmAI GTCTC 3 cut(s) 96, 157, 656
BsmBI CGTCTC 1 cut(s) 157
BsnI GGCC 3 cut(s) 621, 801, 935
Bso31I GGTCTC 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 86, 1059
Bsp143I GATC 2 cut(s) 337, 502
Bsp19I CCATGG 1 cut(s) 131
BspACI CCGC 1 cut(s) 95
BspANI GGCC 3 cut(s) 621, 801, 935
BspCNI CTCAG 3 cut(s) 63, 571, 903
BspLI GGNNCC 2 cut(s) 751, 1007
BspMAI CTGCAG 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 956
BspT107I GGYRCC 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 96
BsrI ACTGG 2 cut(s) 402, 414
BssECI CCNNGG 1 cut(s) 131
BssMI GATC 2 cut(s) 337, 502
BssNI GRCGYC 1 cut(s) 750
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 579, 641
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 1 cut(s) 750
BstAPI GCANNNNNTGC 1 cut(s) 634
BstC8I GCNNGC 5 cut(s) 409, 545, 623, 867, 871
BstDEI CTNAG 3 cut(s) 71, 558, 890
BstDSI CCRYGG 1 cut(s) 131
BstEII GGTNACC 1 cut(s) 913
BstF5I GGATG 2 cut(s) 226, 989
BstH2I RGCGCY 1 cut(s) 753
BstHHI GCGC 3 cut(s) 472, 752, 865
BstKTI GATC 2 cut(s) 340, 505
BstMAI GTCTC 3 cut(s) 96, 157, 656
BstMBI GATC 2 cut(s) 337, 502
BstMWI GCNNNNNNNGC 2 cut(s) 253, 634
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 251
BstPI GGTNACC 1 cut(s) 913
BstSCI CCNGG 1 cut(s) 474
BstSFI CTRYAG 1 cut(s) 623
BstSLI GKGCMC 1 cut(s) 1059
BstV1I GCAGC 3 cut(s) 159, 350, 637
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 3 cut(s) 621, 801, 935
BtgI CCRYGG 1 cut(s) 131
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 2 cut(s) 226, 989
BtsIMutI CAGTG 4 cut(s) 409, 687, 837, 941
BveI ACCTGC 1 cut(s) 956
Cac8I GCNNGC 5 cut(s) 409, 545, 623, 867, 871
CaiI CAGNNNCTG 2 cut(s) 617, 760
CfoI GCGC 3 cut(s) 472, 752, 865
Cfr13I GGNCC 2 cut(s) 799, 934
CsiI ACCWGGT 1 cut(s) 474
Csp6I GTAC 2 cut(s) 670, 830
CviAII CATG 3 cut(s) 132, 248, 822
CviQI GTAC 2 cut(s) 670, 830
DdeI CTNAG 3 cut(s) 71, 558, 890
DinI GGCGCC 1 cut(s) 751
DpnI GATC 2 cut(s) 339, 504
DpnII GATC 2 cut(s) 337, 502
DraIII CACNNNGTG 1 cut(s) 478
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 96
Eco32I GATATC 2 cut(s) 242, 375
Eco57I CTGAAG 1 cut(s) 1007
Eco91I GGTNACC 1 cut(s) 913
EcoO109I RGGNCCY 2 cut(s) 799, 934
EcoO65I GGTNACC 1 cut(s) 913
EcoRII CCWGG 1 cut(s) 474
EcoRV GATATC 2 cut(s) 242, 375
EcoT14I CCWWGG 1 cut(s) 131
EgeI GGCGCC 1 cut(s) 751
EheI GGCGCC 1 cut(s) 751
ErhI CCWWGG 1 cut(s) 131
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 3 cut(s) 135, 251, 825
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 3 cut(s) 131, 247, 821
Fnu4HI GCNGC 4 cut(s) 95, 173, 364, 626
FokI GGATG 2 cut(s) 233, 996
Fsp4HI GCNGC 4 cut(s) 95, 173, 364, 626
FspBI CTAG 3 cut(s) 122, 648, 789
FspI TGCGCA 1 cut(s) 864
GlaI GCGC 3 cut(s) 471, 751, 864
GluI GCNGC 4 cut(s) 95, 173, 364, 626
GsaI CCCAGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 753
HaeIII GGCC 3 cut(s) 621, 801, 935
HhaI GCGC 3 cut(s) 472, 752, 865
Hin1I GRCGYC 1 cut(s) 750
Hin1II CATG 3 cut(s) 135, 251, 825
Hin6I GCGC 3 cut(s) 470, 750, 863
HinP1I GCGC 3 cut(s) 470, 750, 863
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HindIII AAGCTT 3 cut(s) 541, 682, 696
HinfI GANTC 5 cut(s) 208, 326, 352, 614, 757
HpaI GTTAAC 1 cut(s) 609
HphI GGTGA 3 cut(s) 38, 490, 925
Hpy166II GTNNAC 3 cut(s) 609, 830, 1057
Hpy188I TCNGA 7 cut(s) 72, 379, 507, 535, 561, 766, 1026
Hpy188III TCNNGA 9 cut(s) 179, 188, 299, 323, 335, 341, 569, 881, 971
Hpy8I GTNNAC 3 cut(s) 609, 830, 1057
HpyAV CCTTC 4 cut(s) 171, 415, 764, 812
HpyCH4III ACNGT 2 cut(s) 579, 641
HpyCH4IV ACGT 2 cut(s) 510, 843
HpyCH4V TGCA 9 cut(s) 175, 275, 489, 547, 625, 637, 795, 873, 1057
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 634
HpyF3I CTNAG 3 cut(s) 71, 558, 890
HpySE526I ACGT 2 cut(s) 510, 843
Hsp92I GRCGYC 1 cut(s) 750
Hsp92II CATG 3 cut(s) 135, 251, 825
HspAI GCGC 3 cut(s) 470, 750, 863
KasI GGCGCC 1 cut(s) 749
KspAI GTTAAC 1 cut(s) 609
Kzo9I GATC 2 cut(s) 337, 502
LmnI GCTCC 2 cut(s) 45, 1005
Lsp1109I GCAGC 3 cut(s) 159, 350, 637
LweI GCATC 5 cut(s) 184, 211, 304, 782, 1034
MabI ACCWGGT 1 cut(s) 474
MaeI CTAG 3 cut(s) 122, 648, 789
MaeII ACGT 2 cut(s) 510, 843
MaeIII GTNAC 4 cut(s) 451, 835, 913, 1038
MalI GATC 2 cut(s) 339, 504
MboI GATC 2 cut(s) 337, 502
MboII GAAGA 4 cut(s) 299, 323, 382, 493
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 86, 1059
Mly113I GGCGCC 1 cut(s) 750
MnlI CCTC 6 cut(s) 22, 25, 116, 337, 441, 925
MroXI GAANNNNTTC 2 cut(s) 57, 1020
MseI TTAA 6 cut(s) 357, 551, 608, 729, 978, 996
MslI CAYNNNNRTG 1 cut(s) 842
MspR9I CCNGG 1 cut(s) 476
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 2 cut(s) 253, 634
NarI GGCGCC 1 cut(s) 750
NcoI CCATGG 1 cut(s) 131
NdeII GATC 2 cut(s) 337, 502
NlaIII CATG 3 cut(s) 135, 251, 825
NlaIV GGNNCC 2 cut(s) 751, 1007
NmuCI GTSAC 2 cut(s) 835, 913
NsbI TGCGCA 1 cut(s) 864
NspI RCATGY 1 cut(s) 251
OliI CACNNNNGTG 1 cut(s) 842
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdmI GAANNNNTTC 2 cut(s) 57, 1020
PfeI GAWTC 5 cut(s) 208, 326, 352, 614, 757
PkrI GCNGC 4 cut(s) 96, 174, 365, 627
PluTI GGCGCC 1 cut(s) 753
PshBI ATTAAT 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 2 cut(s) 751, 1007
PspPI GGNCC 2 cut(s) 799, 934
PstI CTGCAG 1 cut(s) 627
PstNI CAGNNNCTG 2 cut(s) 617, 760
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 2 cut(s) 671, 831
RsaNI GTAC 2 cut(s) 670, 830
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 6 cut(s) 357, 551, 608, 729, 978, 996
SatI GCNGC 4 cut(s) 95, 173, 364, 626
Sau3AI GATC 2 cut(s) 337, 502
Sau96I GGNCC 2 cut(s) 799, 934
ScrFI CCNGG 1 cut(s) 476
SduI GDGCHC 2 cut(s) 86, 1059
SexAI ACCWGGT 1 cut(s) 474
SfaNI GCATC 5 cut(s) 184, 211, 304, 782, 1034
SfcI CTRYAG 1 cut(s) 623
SfoI GGCGCC 1 cut(s) 751
SmiMI CAYNNNNRTG 1 cut(s) 842
SsiI CCGC 1 cut(s) 95
SspDI GGCGCC 1 cut(s) 749
SspMI CTAG 3 cut(s) 122, 648, 789
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 2 cut(s) 579, 641
TaiI ACGT 2 cut(s) 513, 846
TaqI TCGA 1 cut(s) 187
TaqII GACCGA 1 cut(s) 932
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 5 cut(s) 208, 326, 352, 614, 757
Tru1I TTAA 6 cut(s) 357, 551, 608, 729, 978, 996
Tru9I TTAA 6 cut(s) 357, 551, 608, 729, 978, 996
TscAI CASTG 4 cut(s) 409, 694, 844, 948
TseFI GTSAC 2 cut(s) 835, 913
TseI GCWGC 3 cut(s) 172, 363, 625
Tsp45I GTSAC 2 cut(s) 835, 913
TspDTI ATGAA 6 cut(s) 209, 219, 365, 708, 783, 1029
TspGWI ACGGA 1 cut(s) 1018
TspRI CASTG 4 cut(s) 409, 694, 844, 948
VneI GTGCAC 1 cut(s) 1055
VspI ATTAAT 1 cut(s) 551
XapI RAATTY 5 cut(s) 269, 425, 664, 731, 884
XceI RCATGY 1 cut(s) 251
XmnI GAANNNNTTC 2 cut(s) 57, 1020
XspI CTAG 3 cut(s) 122, 648, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.