Rh3AG220400

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011155
Physical Location & Seq
Forward (+)
22526310 .. 22529966
3657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG220400.1

Sequence Viewer

Length: 732 bp
ATGGAAGTAGAAGGAGGTGAGGAGGTGACATGGAGCAGAGAAACAGTTCCCAAGGTTGTGAAGATAGTGAGCACAAGACTCCCTCAGAGAGACCTCATCTCTCTCTTGCTAGTCAGCCCATGGCTCAACGCTACTCTCATCTCCCACCCTTCCCTTTGGCTGCTTATCGATTTTCGTGAAATGAATAATGCTGGGAATCGCCTAATAGCTGCTCTGTCACTGCCGCGATATCGGCATGTGAAGCAGATAAACCTTGAATTTGCACAAGATATTGAAGACAAACATCTTGAACTCATAAAGAACAAGTGTTTTGGTTCTCTTCAAAACCTTGAAGTTTTGAATCTGAATAGCTGCCAGAAGATTTCTGATAAGGGAGTTCAAGATATAACCAGTGCTTGTGCCAATCTGAAGGTCTTTTCTATTTATTGGAATGTGAGGGTGACAGATATAGGTATAACACACCTGGTGAAAAATTGCAAGTATATCATTGATCTGAATATAAGTGGCTGTAAGAATATTTTAGACAAAAGTTTGCAACTGGTTGCTCAGACTTATCCAGAATTAGAGTTGCTGAATCTGACAAGGTGTGTCAAGTTAACAGATTTTGGCTTGCAGCAGATATTGAACAGTTGCTCCAGTCTCCAGAGTCTCAACTTATACGCCCTGTCTAGGTATTTGGTGGAGTCTTTGCTTACTTTAAACTATGTTTTCTTGCTGTGTATTGATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

243

Amino Acids

27.61

Weight (kDa)

6.88

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 105 - 217 2.1e-07 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58530
fragaria_vesca FvH4_6g21620 FvH4_6g21620
malus_domestica MD12G1053200.v1.1 MD14G1051600.v1.1
prunus_persica Prupe.7G084400_v2.0.a1
pyrus_communis pycom12g04660 pycom14g04290 pycom14g04300
rosa_chinensis RchiOBHm_Chr3g0478871
rosa_laevigata RLG00000023619
rosa_multiflora Rmu_sc0004831.1_g000004 Rmu_sc0005751.1_g000013
rosa_roxburghii Rroxscaffold_6G00403010
rosa_rugosa Rorug03G0168800
rosa_samantha Rh3AG220400 Rh3BG252300 Rh3CG249100 Rh3DG245300
rosa_wichuraiana Rw3G019820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 226
AciI CCGC 1 cut(s) 224
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 1 cut(s) 428
AdeI CACNNNGTG 1 cut(s) 466
AfiI CCNNNNNNNGG 1 cut(s) 669
AgsI TTSAA 8 cut(s) 257, 275, 290, 323, 332, 340, 380, 625
AjnI CCWGG 1 cut(s) 462
AloI GAACNNNNNNTCC 2 cut(s) 617, 649
AluBI AGCT 2 cut(s) 209, 351
AluI AGCT 2 cut(s) 209, 351
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 3 cut(s) 84, 644, 653
ApeKI GCWGC 4 cut(s) 160, 209, 351, 613
ApoI RAATTY 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 45
AsuHPI GGTGA 4 cut(s) 29, 37, 451, 478
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 4 cut(s) 147, 196, 338, 625
BciT130I CCWGG 1 cut(s) 464
BcoDI GTCTC 3 cut(s) 84, 644, 653
BfaI CTAG 2 cut(s) 110, 669
BisI GCNGC 5 cut(s) 161, 210, 224, 352, 614
BlsI GCNGC 5 cut(s) 162, 211, 225, 353, 615
Bme1390I CCNGG 1 cut(s) 464
BmrFI CCNGG 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 619, 626
Bsa29I ATCGAT 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 51, 119
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 1 cut(s) 669
Bse1I ACTGG 3 cut(s) 390, 543, 636
BseBI CCWGG 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 51, 119
BseLI CCNNNNNNNGG 1 cut(s) 669
BseMII CTCAG 2 cut(s) 98, 560
BseNI ACTGG 3 cut(s) 390, 543, 636
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 4 cut(s) 147, 196, 338, 625
BseYI CCCAGC 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 226
BshVI ATCGAT 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 669
BsmAI GTCTC 3 cut(s) 84, 644, 653
Bso31I GGTCTC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 1 cut(s) 490
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 1 cut(s) 224
BspCNI CTCAG 2 cut(s) 97, 559
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 84
BsrI ACTGG 3 cut(s) 390, 543, 636
BssECI CCNNGG 2 cut(s) 51, 119
BssMI GATC 1 cut(s) 490
BssT1I CCWWGG 2 cut(s) 51, 119
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 46, 629
Bst6I CTCTTC 1 cut(s) 324
BstC8I GCNNGC 1 cut(s) 611
BstDEI CTNAG 3 cut(s) 84, 546, 729
BstDSI CCRYGG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 226
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 3 cut(s) 84, 644, 653
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 2 cut(s) 232, 241
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 462
BstUI CGCG 1 cut(s) 226
BstV1I GCAGC 4 cut(s) 147, 196, 338, 625
BstV2I GAAGAC 1 cut(s) 282
Bsu15I ATCGAT 1 cut(s) 168
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 119
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 2 cut(s) 218, 397
Cac8I GCNNGC 1 cut(s) 611
ClaI ATCGAT 1 cut(s) 168
CsiI ACCWGGT 1 cut(s) 462
CviAII CATG 3 cut(s) 30, 120, 236
CviJI RGCY 7 cut(s) 117, 124, 160, 209, 351, 507, 609
CviKI_1 RGCY 7 cut(s) 117, 124, 160, 209, 351, 507, 609
DdeI CTNAG 3 cut(s) 84, 546, 729
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
DraI TTTAAA 1 cut(s) 699
DraIII CACNNNGTG 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 51, 119
Eco31I GGTCTC 1 cut(s) 84
Eco32I GATATC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 230
EcoT14I CCWWGG 2 cut(s) 51, 119
ErhI CCWWGG 2 cut(s) 51, 119
FaeI CATG 3 cut(s) 33, 123, 239
FatI CATG 3 cut(s) 29, 119, 235
Fnu4HI GCNGC 5 cut(s) 161, 210, 224, 352, 614
Fsp4HI GCNGC 5 cut(s) 161, 210, 224, 352, 614
FspBI CTAG 2 cut(s) 110, 669
GluI GCNGC 5 cut(s) 161, 210, 224, 352, 614
GsaI CCCAGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 619, 626
Hin1II CATG 3 cut(s) 33, 123, 239
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HinfI GANTC 7 cut(s) 78, 196, 340, 574, 646, 683, 725
HpaI GTTAAC 1 cut(s) 597
HphI GGTGA 4 cut(s) 29, 37, 451, 478
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 7 cut(s) 87, 345, 367, 408, 495, 549, 579
Hpy188III TCNNGA 5 cut(s) 176, 287, 380, 557, 643
Hpy8I GTNNAC 1 cut(s) 597
HpyAV CCTTC 3 cut(s) 5, 159, 403
HpyCH4III ACNGT 2 cut(s) 46, 629
HpyCH4V TGCA 4 cut(s) 263, 477, 535, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 241
HpyF3I CTNAG 3 cut(s) 84, 546, 729
Hsp92II CATG 3 cut(s) 33, 123, 239
KspAI GTTAAC 1 cut(s) 597
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 33, 638
Lsp1109I GCAGC 4 cut(s) 147, 196, 338, 625
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 2 cut(s) 110, 669
MaeIII GTNAC 3 cut(s) 25, 216, 439
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 4 cut(s) 73, 287, 311, 370
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 3 cut(s) 257, 472, 560
MlyI GAGTC 3 cut(s) 72, 655, 692
MnlI CCTC 6 cut(s) 8, 13, 16, 93, 104, 429
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 2 cut(s) 596, 698
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 2 cut(s) 232, 241
NcoI CCATGG 1 cut(s) 119
NdeII GATC 1 cut(s) 490
NlaIII CATG 3 cut(s) 33, 123, 239
NmuCI GTSAC 3 cut(s) 25, 216, 439
NspI RCATGY 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 4 cut(s) 196, 340, 574, 725
PkrI GCNGC 5 cut(s) 162, 211, 225, 353, 615
PleI GAGTC 3 cut(s) 72, 654, 691
PpsI GAGTC 3 cut(s) 72, 654, 691
Psp6I CCWGG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 462
SaqAI TTAA 2 cut(s) 596, 698
SatI GCNGC 5 cut(s) 161, 210, 224, 352, 614
Sau3AI GATC 1 cut(s) 490
SchI GAGTC 3 cut(s) 72, 655, 692
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 74
SexAI ACCWGGT 1 cut(s) 462
Sse9I AATT 3 cut(s) 257, 472, 560
SsiI CCGC 1 cut(s) 224
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 110, 669
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 2 cut(s) 51, 119
TaaI ACNGT 2 cut(s) 46, 629
TaqI TCGA 1 cut(s) 168
TasI AATT 3 cut(s) 257, 472, 560
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 4 cut(s) 196, 340, 574, 725
Tru1I TTAA 2 cut(s) 596, 698
Tru9I TTAA 2 cut(s) 596, 698
TscAI CASTG 2 cut(s) 225, 397
TseFI GTSAC 3 cut(s) 25, 216, 439
TseI GCWGC 4 cut(s) 160, 209, 351, 613
Tsp45I GTSAC 3 cut(s) 25, 216, 439
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 2 cut(s) 225, 397
XapI RAATTY 1 cut(s) 257
XceI RCATGY 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 2 cut(s) 110, 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.