pycom12g04660

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011155
Physical Location & Seq
Forward (+)
4494930 .. 4499782
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04660.10

Sequence Viewer

Length: 1137 bp
ATGGAAGAGAAGCAAGTAGAAGAAGCAGGTGGGGAGGAGGTGGCTTGGAGCAGAGAAACCATTCCCAAAGTTCTGAAAATAGTTAGCACAAGGCTGCCTCAGAGGGACCTCATCTCTCTCTTGCTAGTCAACCCATGGCTCAACGCAACTCTCATCTCCTACCCTTCCCTTTGGCTGCTTCTGGAACTTCGAGAGATGAATAATGCTGGGAATCGCCTTGTGGATGCTCTTTCATTGGCGAGGTATCGGCATGTGAAGCAGATAAACCTTGAATTTTCACAAGACATTGAAGACAAGCATCTTGAACTCGTAAAGAGCAAGTGTCGTGATTCTCTTCAAAACCTGGAGGTTTTGAATCTTAATGGCTGCCAGAAGATCTCTGATAAGGGAATTGAAGCTATAACCAGTTCTTGCCCCAATCTGAAGGAGTTTTCAATCTATTGGAATGTGAGGGCAACAGATCTAGGTATAACGCACCTGGTGAAAAACTGCAAGCATATAGTTGATCTGAATTTAAGTGGCTGTAAGAATCTTTCAGACAAAAGCTTGCAATTAGTTGCTGAGAATTATCCAGAATTACAGTTGTTGAACCTGACGAGGTGTGTCAAGTTAACAGATTCTGGCTTGCAGCAGATATTGCACGGTTGTCGTTGTCTCCATAGTCTAAATTTGTATGCCCTGTCAAGCTTCACTGATGAAGCTTACAAGAAAATATCACTTCTTTCCCATCTTCAATTCTTGGATTTATGCGGTGCTCAGAATCTATCGGATGAAGGGGTGTCTTGTATAGCTAGATGCAAGAGCCTTTTGTCTCTCAATTTGACATGGTGTGTACGGGTCACTGATGTGGGGGTCATAGCTGTTGCGCAGGGTTGTACGTCGCTTGAATTTCTCAGCTTATTTGGGATAGTTGGGGTGACTGATAAATGCTTGGAGTCCCTTTCACTGACCTGCTCAAACACACTTACGACTCTTGATGTTAATGGATGTATTGGAATTAAGATGAACAGGATTACTCTTCGAAGTTGCATTGATAGTCAATCGACAGACATGCGAGGGTGGAACCGTGGAAGAGTTAGTGCAGACTTTCGGTATTTCATGCAGCTTGACTATACTCTGATCTTCGGTGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

379

Amino Acids

42.37

Weight (kDa)

6.2

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58530
fragaria_vesca FvH4_6g21620 FvH4_6g21620
malus_domestica MD12G1053200.v1.1 MD14G1051600.v1.1
prunus_persica Prupe.7G084400_v2.0.a1
pyrus_communis pycom12g04660 pycom14g04290 pycom14g04300
rosa_chinensis RchiOBHm_Chr3g0478871
rosa_laevigata RLG00000023619
rosa_multiflora Rmu_sc0004831.1_g000004 Rmu_sc0005751.1_g000013
rosa_roxburghii Rroxscaffold_6G00403010
rosa_rugosa Rorug03G0168800
rosa_samantha Rh3AG220400 Rh3BG252300 Rh3CG249100 Rh3DG245300
rosa_wichuraiana Rw3G019820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 867
Acc36I ACCTGC 2 cut(s) 17, 959
AciI CCGC 1 cut(s) 750
AcsI RAATTY 4 cut(s) 272, 511, 667, 887
AcuI CTGAAG 1 cut(s) 443
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 2 cut(s) 834, 877
AjnI CCWGG 2 cut(s) 342, 477
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 8 cut(s) 398, 546, 687, 701, 791, 860, 897, 1105
AluI AGCT 8 cut(s) 398, 546, 687, 701, 791, 860, 897, 1105
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 2 cut(s) 659, 816
AlwNI CAGNNNCTG 1 cut(s) 620
ApeKI GCWGC 5 cut(s) 94, 175, 366, 628, 1102
ApoI RAATTY 4 cut(s) 272, 511, 667, 887
Asp700I GAANNNNTTC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 493, 928
AsuII TTCGAA 1 cut(s) 1021
AvaII GGWCC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 5 cut(s) 81, 162, 353, 640, 1114
BccI CCATC 1 cut(s) 735
BcgI CGANNNNNNTGC 4 cut(s) 629, 663, 1033, 1067
BciT130I CCWGG 2 cut(s) 344, 479
BcoDI GTCTC 2 cut(s) 659, 816
BfaI CTAG 3 cut(s) 125, 464, 792
BfuAI ACCTGC 2 cut(s) 17, 959
BglII AGATCT 2 cut(s) 375, 460
BisI GCNGC 5 cut(s) 95, 176, 367, 629, 1103
BlsI GCNGC 5 cut(s) 96, 177, 368, 630, 1104
Bme1390I CCNGG 2 cut(s) 344, 479
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 2 cut(s) 107, 1064
BmrFI CCNGG 2 cut(s) 344, 479
BmsI GCATC 3 cut(s) 214, 307, 785
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 1021
BsaJI CCNNGG 2 cut(s) 134, 1066
Bse1I ACTGG 1 cut(s) 405
BseBI CCWGG 2 cut(s) 344, 479
BseDI CCNNGG 2 cut(s) 134, 1066
BseGI GGATG 3 cut(s) 229, 775, 992
BseMII CTCAG 4 cut(s) 113, 552, 770, 907
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 5 cut(s) 81, 162, 353, 640, 1114
BseYI CCCAGC 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 1101
BsiHKAI GWGCWC 1 cut(s) 757
BslFI GGGAC 2 cut(s) 119, 922
BsmAI GTCTC 2 cut(s) 659, 816
BsmFI GGGAC 2 cut(s) 119, 922
Bsp119I TTCGAA 1 cut(s) 1021
Bsp1286I GDGCHC 1 cut(s) 757
Bsp143I GATC 4 cut(s) 375, 460, 505, 1119
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 1 cut(s) 750
BspCNI CTCAG 4 cut(s) 112, 553, 769, 906
BspLI GGNNCC 2 cut(s) 107, 1064
BspMI ACCTGC 2 cut(s) 17, 959
BspT104I TTCGAA 1 cut(s) 1021
BsrI ACTGG 1 cut(s) 405
BssECI CCNNGG 2 cut(s) 134, 1066
BssMI GATC 4 cut(s) 375, 460, 505, 1119
BssT1I CCWWGG 1 cut(s) 134
Bst2UI CCWGG 2 cut(s) 344, 479
Bst4CI ACNGT 3 cut(s) 582, 644, 1067
Bst6I CTCTTC 3 cut(s) 339, 1023, 1066
BstAPI GCANNNNNTGC 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 1021
BstC8I GCNNGC 3 cut(s) 494, 548, 626
BstDEI CTNAG 4 cut(s) 99, 561, 756, 893
BstDSI CCRYGG 2 cut(s) 134, 1066
BstF5I GGATG 3 cut(s) 229, 775, 992
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 4 cut(s) 378, 463, 508, 1122
BstMAI GTCTC 2 cut(s) 659, 816
BstMBI GATC 4 cut(s) 375, 460, 505, 1119
BstMWI GCNNNNNNNGC 2 cut(s) 256, 637
BstNI CCWGG 2 cut(s) 344, 479
BstNSI RCATGY 2 cut(s) 254, 1054
BstSCI CCNGG 2 cut(s) 342, 477
BstV1I GCAGC 5 cut(s) 81, 162, 353, 640, 1114
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 2 cut(s) 375, 460
BstYI RGATCY 2 cut(s) 375, 460
BtgI CCRYGG 2 cut(s) 134, 1066
BtsCI GGATG 3 cut(s) 229, 775, 992
BtsIMutI CAGTG 3 cut(s) 690, 840, 944
BveI ACCTGC 2 cut(s) 17, 959
Cac8I GCNNGC 3 cut(s) 494, 548, 626
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 868
Cfr13I GGNCC 1 cut(s) 106
CsiI ACCWGGT 1 cut(s) 477
Csp6I GTAC 2 cut(s) 833, 876
CviAII CATG 5 cut(s) 135, 251, 825, 1051, 1099
CviQI GTAC 2 cut(s) 833, 876
DdeI CTNAG 4 cut(s) 99, 561, 756, 893
DpnI GATC 4 cut(s) 377, 462, 507, 1121
DpnII GATC 4 cut(s) 375, 460, 505, 1119
DraIII CACNNNGTG 1 cut(s) 481
Eam1104I CTCTTC 3 cut(s) 339, 1023, 1066
EarI CTCTTC 3 cut(s) 339, 1023, 1066
Eco130I CCWWGG 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 342, 477
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 5 cut(s) 138, 254, 828, 1054, 1102
FaqI GGGAC 2 cut(s) 119, 922
FatI CATG 5 cut(s) 134, 250, 824, 1050, 1098
Fnu4HI GCNGC 5 cut(s) 95, 176, 367, 629, 1103
FokI GGATG 3 cut(s) 236, 782, 999
Fsp4HI GCNGC 5 cut(s) 95, 176, 367, 629, 1103
FspBI CTAG 3 cut(s) 125, 464, 792
FspI TGCGCA 1 cut(s) 867
GlaI GCGC 1 cut(s) 867
GluI GCNGC 5 cut(s) 95, 176, 367, 629, 1103
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 365
HhaI GCGC 1 cut(s) 868
Hin1II CATG 5 cut(s) 138, 254, 828, 1054, 1102
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HincII GTYRAC 2 cut(s) 130, 612
HindII GTYRAC 2 cut(s) 130, 612
HindIII AAGCTT 3 cut(s) 544, 685, 699
HinfI GANTC 9 cut(s) 211, 329, 355, 529, 617, 760, 935, 970, 1130
HpaI GTTAAC 1 cut(s) 612
HphI GGTGA 2 cut(s) 493, 928
Hpy166II GTNNAC 3 cut(s) 130, 612, 833
Hpy188I TCNGA 9 cut(s) 75, 102, 382, 423, 510, 538, 759, 769, 1119
Hpy188III TCNNGA 6 cut(s) 182, 191, 302, 326, 572, 974
Hpy8I GTNNAC 3 cut(s) 130, 612, 833
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 3 cut(s) 174, 418, 767
HpyCH4III ACNGT 3 cut(s) 582, 644, 1067
HpyCH4IV ACGT 1 cut(s) 878
HpyCH4V TGCA 8 cut(s) 492, 550, 628, 640, 798, 1029, 1082, 1102
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 637
HpyF3I CTNAG 4 cut(s) 99, 561, 756, 893
HpySE526I ACGT 1 cut(s) 878
Hsp92II CATG 5 cut(s) 138, 254, 828, 1054, 1102
HspAI GCGC 1 cut(s) 866
KspAI GTTAAC 1 cut(s) 612
Kzo9I GATC 4 cut(s) 375, 460, 505, 1119
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 5 cut(s) 81, 162, 353, 640, 1114
LweI GCATC 3 cut(s) 214, 307, 785
MabI ACCWGGT 1 cut(s) 477
MaeI CTAG 3 cut(s) 125, 464, 792
MaeII ACGT 1 cut(s) 878
MaeIII GTNAC 2 cut(s) 838, 916
MalI GATC 4 cut(s) 377, 462, 507, 1121
MboI GATC 4 cut(s) 375, 460, 505, 1119
MboII GAAGA 9 cut(s) 17, 32, 302, 326, 385, 722, 1010, 1083, 1114
MflI RGATCY 2 cut(s) 375, 460
MhlI GDGCHC 1 cut(s) 757
MlyI GAGTC 2 cut(s) 944, 964
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 6 cut(s) 360, 515, 611, 981, 999, 1135
MslI CAYNNNNRTG 1 cut(s) 845
MspR9I CCNGG 2 cut(s) 344, 479
MvaI CCWGG 2 cut(s) 344, 479
MwoI GCNNNNNNNGC 2 cut(s) 256, 637
NcoI CCATGG 1 cut(s) 134
NdeII GATC 4 cut(s) 375, 460, 505, 1119
NlaIII CATG 5 cut(s) 138, 254, 828, 1054, 1102
NlaIV GGNNCC 2 cut(s) 107, 1064
NmuCI GTSAC 2 cut(s) 838, 916
NsbI TGCGCA 1 cut(s) 867
NspI RCATGY 2 cut(s) 254, 1054
NspV TTCGAA 1 cut(s) 1021
OliI CACNNNNGTG 1 cut(s) 845
PaqCI CACCTGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 7 cut(s) 211, 329, 355, 529, 617, 760, 1130
PkrI GCNGC 5 cut(s) 96, 177, 368, 630, 1104
PleI GAGTC 2 cut(s) 943, 964
PpsI GAGTC 2 cut(s) 943, 964
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 2 cut(s) 342, 477
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 342, 477
PspN4I GGNNCC 2 cut(s) 107, 1064
PspPI GGNCC 1 cut(s) 106
PspPPI RGGWCCY 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 2 cut(s) 375, 460
RsaI GTAC 2 cut(s) 834, 877
RsaNI GTAC 2 cut(s) 833, 876
RseI CAYNNNNRTG 1 cut(s) 845
SaqAI TTAA 6 cut(s) 360, 515, 611, 981, 999, 1135
SatI GCNGC 5 cut(s) 95, 176, 367, 629, 1103
Sau3AI GATC 4 cut(s) 375, 460, 505, 1119
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 2 cut(s) 944, 964
ScrFI CCNGG 2 cut(s) 344, 479
SduI GDGCHC 1 cut(s) 757
SexAI ACCWGGT 1 cut(s) 477
SfaNI GCATC 3 cut(s) 214, 307, 785
SfuI TTCGAA 1 cut(s) 1021
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 845
SsiI CCGC 1 cut(s) 750
SspMI CTAG 3 cut(s) 125, 464, 792
StyD4I CCNGG 2 cut(s) 342, 477
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 3 cut(s) 582, 644, 1067
TaiI ACGT 1 cut(s) 881
TaqI TCGA 3 cut(s) 190, 1021, 1043
TfiI GAWTC 7 cut(s) 211, 329, 355, 529, 617, 760, 1130
Tru1I TTAA 6 cut(s) 360, 515, 611, 981, 999, 1135
Tru9I TTAA 6 cut(s) 360, 515, 611, 981, 999, 1135
TscAI CASTG 3 cut(s) 697, 847, 951
TseFI GTSAC 2 cut(s) 838, 916
TseI GCWGC 5 cut(s) 94, 175, 366, 628, 1102
Tsp45I GTSAC 2 cut(s) 838, 916
TspDTI ATGAA 6 cut(s) 212, 222, 711, 786, 1019, 1087
TspRI CASTG 3 cut(s) 697, 847, 951
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 4 cut(s) 272, 511, 667, 887
XceI RCATGY 2 cut(s) 254, 1054
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 3 cut(s) 125, 464, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.