MD12G1058500.v1.1

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011942
Physical Location & Seq
Forward (+)
6800218 .. 6800769
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1058500.v1.1.491

Sequence Viewer

Length: 552 bp
ATGAAGATCAAAACCCTAGTCCACACCCTCATTTTCTCACACATGTGCCGCGTAATTCGGTGCCTCAACAAAGCCAGGTCCAAAGCCTTTTCAATTCTCATTCAGGTTCTCAGAAATAGGCAACCCATGCAGCTCATCTACCCCACCAAAAAAACCAAGAACAAGAACAAGACCAAGAAGATATTCTTCGGCTCGTTTAGACTGCACTACAACTGGTCTACGCATGTGTTGCCCGTGCCTGCGTGTGTCTACGATGGATTAACGGCAACAAATTTGTACTATGATTCTACATGGAATTCAGTACTTTCAACTGATGGCCAGCAATGTGAGGATAATCATGGTGCTGAATCACAGCTCTCTGGGTACCTCCAATGGCTTGAGGAGAAGGTGGTCCCTGATAATAATAATAATTCCATGTCCGATGATCAGACTGATGCTAATGAGATCGATAAGCTAGCTGATTTGTTCATTGCTAAAAGCCATGAGCAGTTCATATTGGAGAAGCAGGAGTCCTACAGGAGATTTCAAGAAATGCTTGCCAGAAGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

184

Amino Acids

21.34

Weight (kDa)

9.0

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 147 - 181 8.5e-15 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011942)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42180 AT3G57950 AT5G06790
fragaria_vesca FvH4_6g22530
malus_domestica MD12G1058500.v1.1 MD14G1059200.v1.1
prunus_persica Prupe.7G075500_v2.0.a1
pyrus_communis pycom12g05220 pycom14g04810
rosa_chinensis RchiOBHm_Chr3g0479981
rosa_laevigata RLG00000023523
rosa_multiflora Rmu_sc0010444.1_g000018
rosa_roxburghii Rroxscaffold_6G00401830
rosa_rugosa Rorug03G0179700
rosa_samantha Rh3AG230900 Rh3BG263400 Rh3CG260900 Rh3DG256800
rosa_wichuraiana Rw3G020780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 363
AccB1I GGYRCC 2 cut(s) 60, 363
AccI GTMKAC 2 cut(s) 218, 249
AccII CGCG 1 cut(s) 51
AciI CCGC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 2 cut(s) 271, 295
AfaI GTAC 3 cut(s) 278, 303, 365
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 3 cut(s) 93, 309, 527
AjnI CCWGG 1 cut(s) 74
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AleI CACNNNNGTG 1 cut(s) 43
AluBI AGCT 4 cut(s) 133, 355, 454, 458
AluI AGCT 4 cut(s) 133, 355, 454, 458
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 2 cut(s) 271, 295
ArsI GACNNNNNNTTYG 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 182
Asp718I GGTACC 1 cut(s) 363
AspS9I GGNCC 2 cut(s) 78, 391
AsuNHI GCTAGC 1 cut(s) 454
AvaII GGWCC 2 cut(s) 78, 391
BalI TGGCCA 1 cut(s) 318
BanI GGYRCC 2 cut(s) 60, 363
BbvI GCAGC 1 cut(s) 142
BccI CCATC 2 cut(s) 248, 308
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 1 cut(s) 76
BclI TGATCA 1 cut(s) 424
BfaI CTAG 2 cut(s) 17, 455
BfmI CTRYAG 1 cut(s) 514
BisI GCNGC 2 cut(s) 49, 131
BlsI GCNGC 2 cut(s) 50, 132
BmcAI AGTACT 1 cut(s) 303
Bme1390I CCNGG 1 cut(s) 76
Bme18I GGWCC 2 cut(s) 78, 391
BmgT120I GGNCC 2 cut(s) 78, 391
BmiI GGNNCC 3 cut(s) 62, 365, 393
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 424
BmtI GCTAGC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 218
Bse3DI GCAATG 2 cut(s) 329, 468
BseBI CCWGG 1 cut(s) 76
BseCI ATCGAT 1 cut(s) 447
BseMI GCAATG 2 cut(s) 329, 468
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 1 cut(s) 218
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 2 cut(s) 60, 363
BshVI ATCGAT 1 cut(s) 447
BslFI GGGAC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 318
Bsp143I GATC 3 cut(s) 6, 424, 444
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 123
BspDI ATCGAT 1 cut(s) 447
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 62, 365, 393
BspOI GCTAGC 1 cut(s) 458
BspT107I GGYRCC 2 cut(s) 60, 363
BsrDI GCAATG 2 cut(s) 329, 468
BsrI ACTGG 1 cut(s) 218
BssMI GATC 3 cut(s) 6, 424, 444
Bst2UI CCWGG 1 cut(s) 76
BstAPI GCANNNNNTGC 2 cut(s) 127, 229
BstC8I GCNNGC 4 cut(s) 240, 320, 456, 537
BstDEI CTNAG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 3 cut(s) 9, 427, 447
BstMBI GATC 3 cut(s) 6, 424, 444
BstMWI GCNNNNNNNGC 2 cut(s) 127, 229
BstNI CCWGG 1 cut(s) 76
BstNSI RCATGY 3 cut(s) 46, 227, 550
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 514
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 447
BsuRI GGCC 1 cut(s) 318
BsuTUI ATCGAT 1 cut(s) 447
Cac8I GCNNGC 4 cut(s) 240, 320, 456, 537
Cfr13I GGNCC 2 cut(s) 78, 391
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 3 cut(s) 277, 302, 364
CviAII CATG 8 cut(s) 43, 127, 224, 291, 338, 415, 482, 547
CviQI GTAC 3 cut(s) 277, 302, 364
DdeI CTNAG 1 cut(s) 110
DpnI GATC 3 cut(s) 8, 426, 446
DpnII GATC 3 cut(s) 6, 424, 444
EaeI YGGCCR 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 78, 391
EcoRI GAATTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 74
FaeI CATG 8 cut(s) 46, 130, 227, 294, 341, 418, 485, 550
FalI AAGNNNNNCTT 4 cut(s) 170, 202, 519, 551
FaqI GGGAC 1 cut(s) 377
FatI CATG 8 cut(s) 42, 126, 223, 290, 337, 414, 481, 546
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 2 cut(s) 218, 249
Fnu4HI GCNGC 2 cut(s) 49, 131
Fsp4HI GCNGC 2 cut(s) 49, 131
FspBI CTAG 2 cut(s) 17, 455
GluI GCNGC 2 cut(s) 49, 131
HaeIII GGCC 1 cut(s) 318
Hin1II CATG 8 cut(s) 46, 130, 227, 294, 341, 418, 485, 550
HinfI GANTC 3 cut(s) 284, 347, 509
Hpy166II GTNNAC 3 cut(s) 22, 219, 250
Hpy188I TCNGA 3 cut(s) 113, 421, 429
Hpy188III TCNNGA 1 cut(s) 527
Hpy8I GTNNAC 3 cut(s) 22, 219, 250
HpyAV CCTTC 1 cut(s) 379
HpyCH4V TGCA 2 cut(s) 130, 205
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 229
HpyF3I CTNAG 1 cut(s) 110
Hsp92II CATG 8 cut(s) 46, 130, 227, 294, 341, 418, 485, 550
KpnI GGTACC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 3 cut(s) 6, 424, 444
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 424
MaeI CTAG 2 cut(s) 17, 455
MalI GATC 3 cut(s) 8, 426, 446
MboI GATC 3 cut(s) 6, 424, 444
MboII GAAGA 3 cut(s) 16, 178, 190
MlsI TGGCCA 1 cut(s) 318
MluCI AATT 5 cut(s) 54, 93, 271, 295, 409
MluNI TGGCCA 1 cut(s) 318
MlyI GAGTC 1 cut(s) 518
MnlI CCTC 5 cut(s) 38, 74, 322, 373, 377
Mox20I TGGCCA 1 cut(s) 318
MroXI GAANNNNTTC 1 cut(s) 182
MscI TGGCCA 1 cut(s) 318
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 43
Msp20I TGGCCA 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 127, 229
NdeII GATC 3 cut(s) 6, 424, 444
NheI GCTAGC 1 cut(s) 454
NlaIII CATG 8 cut(s) 46, 130, 227, 294, 341, 418, 485, 550
NlaIV GGNNCC 3 cut(s) 62, 365, 393
NspI RCATGY 3 cut(s) 46, 227, 550
OliI CACNNNNGTG 1 cut(s) 43
PciI ACATGT 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 284, 347
PkrI GCNGC 2 cut(s) 50, 132
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PscI ACATGT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 3 cut(s) 62, 365, 393
PspPI GGNCC 2 cut(s) 78, 391
RsaI GTAC 3 cut(s) 278, 303, 365
RsaNI GTAC 3 cut(s) 277, 302, 364
RseI CAYNNNNRTG 1 cut(s) 43
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 2 cut(s) 49, 131
Sau3AI GATC 3 cut(s) 6, 424, 444
Sau96I GGNCC 2 cut(s) 78, 391
ScaI AGTACT 1 cut(s) 303
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 1 cut(s) 76
SetI ASST 8 cut(s) 80, 108, 135, 357, 369, 390, 456, 460
SfaNI GCATC 1 cut(s) 424
SfcI CTRYAG 1 cut(s) 514
SinI GGWCC 2 cut(s) 78, 391
SmiMI CAYNNNNRTG 1 cut(s) 43
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 5 cut(s) 54, 93, 271, 295, 409
SsiI CCGC 1 cut(s) 49
SspMI CTAG 2 cut(s) 17, 455
StyD4I CCNGG 1 cut(s) 74
TaqI TCGA 1 cut(s) 447
TasI AATT 5 cut(s) 54, 93, 271, 295, 409
TatI WGTACW 2 cut(s) 276, 301
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 2 cut(s) 284, 347
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TseI GCWGC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 17, 457, 481
VpaK11BI GGWCC 2 cut(s) 78, 391
XapI RAATTY 2 cut(s) 271, 295
XceI RCATGY 3 cut(s) 46, 227, 550
XmiI GTMKAC 2 cut(s) 218, 249
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 2 cut(s) 17, 455
ZrmI AGTACT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.