Rorug03G0179700

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011942
Physical Location & Seq
Forward (+)
15380684 .. 15383836
3153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0179700.1

Sequence Viewer

Length: 1656 bp
ATGATGGCGTATAGTAACAAGAAACCGGGCGGCGGCGGCGGTGGTGCTGGTGGTGGTGGTAGTACTGGTTCATATGCATGTTGGTCTTATTCGTCTTACTCAGCAATTTTGGTGCTTATTGTTCCATTGGTTGTGGTTTTCGGGTTGATTTCGCTGTTGAGTCCGAATTCCTCCAATTGGGTTTTCATATCCAACTACTACTTAACCGGCCCTTCTTCGGATACGAGTACCACTAACTCGTCGGAGATCGGAGCTTCACACGACGACGTTTTGGGCTTGAGGTCGACGGTGGTTGTTATGGATATGCACAGTAGTACTGTAGAGGAAGTTCACTCAGATGATTATGTACAGAATCGGTCTGCTTCTCCGCCGCTCGCCGTAGAAGAAGCGACACCACCACCACTTCAACAACCGATTATTAATGGGACAAAGGACGAAGACCCCATGAATGAAACTCATGCTTCTATCATGAATAATCCTGCTACAAGACAAAAGGAGTATACTAGTTTAGGGCGGCTAGAGGGTCGTCTTCAAAGAGCTCGAGTGGCCATAAGAGAGGCTCGATTTGGAAATAAAACACATGATGCAGATTACATTCCTAATGGTCCAATGTATTGGAATGCTAATGCCTTTCACAGGAGCTACTTGGAAATGGAGAAACGGTTTAAAGTATTTGTATATGAAGAAGGGGAACCGCCACTGTTTCATAATGGCCCTTGCAAAAGTATATACTCTATGGAGGGAAATTTTATACATGAGATTGAGGTGAATAAGCAATTTCGGACTAGAAATCCTGAGGAAGCGCATGTTTATTTTCTTCCCTACAGTGTGACAATGTTGGTTCGGTTCGTATATGTGCGTGACTCACATGATTTCAGCCCCATCAGACAGACAGTAAGGGATTATGTCAATATTATCTCCACCAAATATCCCTATTGGAATAGAAGCCTTGCAGCTGATCACTTTACACTTGCTTGCCATGATTGGGGGCCAGAGACTTCAAATTCAGATCCTTATCTGCGCAAATATTCAATCCGTGGTCTATGCAATGCAAATACCTCCGAAGGGTTTAACCCCTCTAAGGATGTCTCCTTTCCAGAAATCAATCTTCAAACTGGTGACACACACGGCTTCCTTGGCGGGCCTTCTCCAAGGCTACGCTCGATATTAGCTTTCTTTGCTGGAGGAGTTCACGGTCCTATTAGGCCTATATTGCTAGAGCATTGGGAAAACAAAGATGAGGAATTGAGGGTGCATCAGTACCTTCCAAAGGGTGTCAACTATTATGACATGATGAGAAAGAGTAAGTTCTGTCTTTGCCCAAGCGGGTACGAAGTTGCAAGCCCAAGGGTGGTTGAGGCAATATACACAGGGTGTGTTCCTGTCCTAATTTCAGATCATTATGTGCCACCTTTCGGTGATGTTCTGAATTGGAAGTCTTTCTCCATTGAGGTTTCAGTGAGTGATATTCCTAACTTGAAGAACATATTGACGAGCATATCTACGAGACAATATCTAAGAATGCAAAGGAGAGTGATACAAGTCAGGAGACATTTTGAGGTCAATTCTCCTCCTAAACGATTTGATGTTTTCCATATGATCCTACATTCTATTTGGCTAAGAAGACTCAATGTTAGAGTACATGATGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

551

Amino Acids

62.39

Weight (kDa)

6.88

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 218 - 500 1.2e-57 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011942)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42180 AT3G57950 AT5G06790
fragaria_vesca FvH4_6g22530
malus_domestica MD12G1058500.v1.1 MD14G1059200.v1.1
prunus_persica Prupe.7G075500_v2.0.a1
pyrus_communis pycom12g05220 pycom14g04810
rosa_chinensis RchiOBHm_Chr3g0479981
rosa_laevigata RLG00000023523
rosa_multiflora Rmu_sc0010444.1_g000018
rosa_roxburghii Rroxscaffold_6G00401830
rosa_rugosa Rorug03G0179700
rosa_samantha Rh3AG230900 Rh3BG263400 Rh3CG260900 Rh3DG256800
rosa_wichuraiana Rw3G020780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1022
AccBSI CCGCTC 1 cut(s) 373
AccI GTMKAC 2 cut(s) 284, 500
AclWI GGATC 2 cut(s) 1004, 1594
AcoI YGGCCR 1 cut(s) 546
AcsI RAATTY 3 cut(s) 166, 745, 1003
AdeI CACNNNGTG 1 cut(s) 1374
AfaI GTAC 7 cut(s) 64, 229, 316, 348, 1262, 1331, 1641
AgsI TTSAA 6 cut(s) 407, 533, 1002, 1032, 1112, 1480
AhlI ACTAGT 1 cut(s) 503
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 5 cut(s) 254, 539, 642, 956, 1172
AluI AGCT 5 cut(s) 254, 539, 642, 956, 1172
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 4 cut(s) 989, 1093, 1501, 1543
AlwI GGATC 2 cut(s) 1004, 1594
Ama87I CYCGRG 1 cut(s) 540
AoxI GGCC 6 cut(s) 208, 546, 712, 989, 1142, 1205
ApeKI GCWGC 1 cut(s) 953
ApoI RAATTY 3 cut(s) 166, 745, 1003
AseI ATTAAT 1 cut(s) 420
Asp700I GAANNNNTTC 1 cut(s) 1439
AspLEI GCGC 2 cut(s) 805, 1023
AspS9I GGNCC 6 cut(s) 209, 605, 713, 989, 1142, 1196
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 3 cut(s) 778, 1130, 1430
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 2 cut(s) 605, 1196
AxyI CCTNAGG 1 cut(s) 795
BalI TGGCCA 1 cut(s) 548
BanII GRGCYC 1 cut(s) 541
BbsI GAAGAC 3 cut(s) 444, 521, 1630
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 965
BccI CCATC 1 cut(s) 890
BceAI ACGGC 2 cut(s) 362, 1144
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 2 cut(s) 958, 1648
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 4 cut(s) 989, 1093, 1501, 1543
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 4 cut(s) 504, 518, 786, 1217
BfmI CTRYAG 2 cut(s) 318, 823
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 6 cut(s) 31, 34, 37, 371, 515, 954
BlsI GCNGC 6 cut(s) 32, 35, 38, 372, 516, 955
BmcAI AGTACT 2 cut(s) 64, 316
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 605, 1196
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 6 cut(s) 209, 605, 713, 989, 1142, 1196
BmiI GGNNCC 2 cut(s) 693, 990
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 574, 1264
BpiI GAAGAC 3 cut(s) 444, 521, 1630
BpmI CTGGAG 1 cut(s) 1203
BpuEI CTTGAG 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 1014
BsaJI CCNNGG 4 cut(s) 1036, 1135, 1151, 1346
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 2 cut(s) 70, 1120
Bse21I CCTNAGG 1 cut(s) 795
Bse3DI GCAATG 1 cut(s) 1054
Bse8I GATNNNNATC 1 cut(s) 1014
BseDI CCNNGG 4 cut(s) 1036, 1135, 1151, 1346
BseGI GGATG 1 cut(s) 1090
BseJI GATNNNNATC 1 cut(s) 1014
BseMI GCAATG 1 cut(s) 1054
BseMII CTCAG 3 cut(s) 114, 348, 786
BseNI ACTGG 2 cut(s) 70, 1120
BseRI GAGGAG 2 cut(s) 1200, 1560
BseXI GCAGC 1 cut(s) 965
BshFI GGCC 6 cut(s) 210, 548, 714, 991, 1144, 1207
BsiHKAI GWGCWC 1 cut(s) 541
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 2 cut(s) 26, 207
BslFI GGGAC 1 cut(s) 439
BsmAI GTCTC 4 cut(s) 989, 1093, 1501, 1543
BsmFI GGGAC 1 cut(s) 439
BsmI GAATGC 2 cut(s) 625, 1527
BsnI GGCC 6 cut(s) 210, 548, 714, 991, 1144, 1207
BsoBI CYCGRG 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
BspANI GGCC 6 cut(s) 210, 548, 714, 991, 1144, 1207
BspCNI CTCAG 3 cut(s) 113, 347, 787
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 693, 990
BspPI GGATC 2 cut(s) 1004, 1594
BsrBI CCGCTC 1 cut(s) 373
BsrDI GCAATG 1 cut(s) 1054
BsrFI RCCGGY 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 2 cut(s) 70, 1120
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 4 cut(s) 1036, 1135, 1151, 1346
BssMI GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
BssNAI GTATAC 1 cut(s) 501
BssT1I CCWWGG 3 cut(s) 1135, 1151, 1346
Bst1107I GTATAC 1 cut(s) 501
Bst4CI ACNGT 8 cut(s) 289, 311, 319, 663, 702, 827, 895, 1196
BstAUI TGTACA 1 cut(s) 346
BstC8I GCNNGC 4 cut(s) 375, 976, 1142, 1342
BstDEI CTNAG 6 cut(s) 100, 334, 795, 1080, 1517, 1619
BstDSI CCRYGG 1 cut(s) 1036
BstENI CCTNNNNNAGG 2 cut(s) 634, 1268
BstF5I GGATG 1 cut(s) 1090
BstHHI GCGC 2 cut(s) 805, 1023
BstKTI GATC 6 cut(s) 249, 961, 1012, 1399, 1602, 1651
BstMAI GTCTC 4 cut(s) 989, 1093, 1501, 1543
BstMBI GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
BstMWI GCNNNNNNNGC 5 cut(s) 36, 545, 1137, 1178, 1213
BstNSI RCATGY 2 cut(s) 81, 809
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 2 cut(s) 318, 823
BstV1I GCAGC 1 cut(s) 965
BstV2I GAAGAC 3 cut(s) 444, 521, 1630
BstX2I RGATCY 1 cut(s) 1009
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 1 cut(s) 1009
BstZ17I GTATAC 1 cut(s) 501
Bsu36I CCTNAGG 1 cut(s) 795
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 6 cut(s) 210, 548, 714, 991, 1144, 1207
BtgI CCRYGG 1 cut(s) 1036
BtsCI GGATG 1 cut(s) 1090
BtsIMutI CAGTG 3 cut(s) 698, 832, 1464
Cac8I GCNNGC 4 cut(s) 375, 976, 1142, 1342
CciI TCATGA 1 cut(s) 468
CfoI GCGC 2 cut(s) 805, 1023
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 6 cut(s) 209, 605, 713, 989, 1142, 1196
Csp6I GTAC 7 cut(s) 63, 228, 315, 347, 1261, 1330, 1640
CviQI GTAC 7 cut(s) 63, 228, 315, 347, 1261, 1330, 1640
DdeI CTNAG 6 cut(s) 100, 334, 795, 1080, 1517, 1619
DpnI GATC 6 cut(s) 248, 960, 1011, 1398, 1601, 1650
DpnII GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
DraI TTTAAA 1 cut(s) 667
DraIII CACNNNGTG 1 cut(s) 1374
EaeI YGGCCR 1 cut(s) 546
EciI GGCGGA 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 539
Eco130I CCWWGG 3 cut(s) 1135, 1151, 1346
Eco147I AGGCCT 1 cut(s) 1207
Eco24I GRGCYC 1 cut(s) 541
Eco47I GGWCC 2 cut(s) 605, 1196
Eco53kI GAGCTC 1 cut(s) 539
Eco81I CCTNAGG 1 cut(s) 795
Eco88I CYCGRG 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 539
EcoNI CCTNNNNNAGG 2 cut(s) 634, 1268
EcoRI GAATTC 1 cut(s) 166
EcoT14I CCWWGG 3 cut(s) 1135, 1151, 1346
EcoT22I ATGCAT 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 3 cut(s) 1135, 1151, 1346
FaqI GGGAC 1 cut(s) 439
FauI CCCGC 2 cut(s) 1133, 1319
FauNDI CATATG 2 cut(s) 73, 1596
FbaI TGATCA 2 cut(s) 958, 1648
FblI GTMKAC 2 cut(s) 284, 500
Fnu4HI GCNGC 6 cut(s) 31, 34, 37, 371, 515, 954
FokI GGATG 1 cut(s) 1097
FriOI GRGCYC 1 cut(s) 541
Fsp4HI GCNGC 6 cut(s) 31, 34, 37, 371, 515, 954
FspBI CTAG 4 cut(s) 504, 518, 786, 1217
FspI TGCGCA 1 cut(s) 1022
GlaI GCGC 2 cut(s) 804, 1022
GluI GCNGC 6 cut(s) 31, 34, 37, 371, 515, 954
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 6 cut(s) 210, 548, 714, 991, 1144, 1207
HapII CCGG 2 cut(s) 26, 207
HhaI GCGC 2 cut(s) 805, 1023
Hin6I GCGC 2 cut(s) 803, 1021
HinP1I GCGC 2 cut(s) 803, 1021
HincII GTYRAC 2 cut(s) 285, 1279
HindII GTYRAC 2 cut(s) 285, 1279
HinfI GANTC 4 cut(s) 160, 352, 863, 1626
HpaII CCGG 2 cut(s) 26, 207
HphI GGTGA 3 cut(s) 778, 1130, 1430
Hpy166II GTNNAC 5 cut(s) 285, 331, 501, 1192, 1279
Hpy188III TCNNGA 4 cut(s) 469, 794, 1097, 1546
Hpy8I GTNNAC 5 cut(s) 285, 331, 501, 1192, 1279
Hpy99I CGWCG 4 cut(s) 244, 266, 269, 289
HpyAV CCTTC 5 cut(s) 222, 680, 1058, 1155, 1274
HpyCH4III ACNGT 8 cut(s) 289, 311, 319, 663, 702, 827, 895, 1196
HpyCH4IV ACGT 1 cut(s) 267
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 545, 1137, 1178, 1213
HpyF3I CTNAG 6 cut(s) 100, 334, 795, 1080, 1517, 1619
HpySE526I ACGT 1 cut(s) 267
HspAI GCGC 2 cut(s) 803, 1021
Ksp22I TGATCA 2 cut(s) 958, 1648
Kzo9I GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
LmnI GCTCC 2 cut(s) 251, 639
Lsp1109I GCAGC 1 cut(s) 965
LweI GCATC 2 cut(s) 574, 1264
MaeI CTAG 4 cut(s) 504, 518, 786, 1217
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 4 cut(s) 14, 829, 860, 1118
MalI GATC 6 cut(s) 248, 960, 1011, 1398, 1601, 1650
MbiI CCGCTC 1 cut(s) 373
MboI GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
MboII GAAGA 9 cut(s) 207, 395, 449, 521, 695, 809, 1100, 1492, 1635
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 541
MlsI TGGCCA 1 cut(s) 548
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 3 cut(s) 169, 857, 1620
MmeI TCCRAC 2 cut(s) 216, 222
Mox20I TGGCCA 1 cut(s) 548
Mph1103I ATGCAT 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 1439
MscI TGGCCA 1 cut(s) 548
MseI TTAA 4 cut(s) 203, 420, 666, 1071
MslI CAYNNNNRTG 2 cut(s) 76, 336
Msp20I TGGCCA 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 956
MspI CCGG 2 cut(s) 26, 207
MspR9I CCNGG 1 cut(s) 27
MunI CAATTG 1 cut(s) 175
Mva1269I GAATGC 2 cut(s) 625, 1527
MwoI GCNNNNNNNGC 5 cut(s) 36, 545, 1137, 1178, 1213
NciI CCSGG 1 cut(s) 27
NdeI CATATG 2 cut(s) 73, 1596
NdeII GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
NlaIV GGNNCC 2 cut(s) 693, 990
NmuCI GTSAC 3 cut(s) 829, 860, 1118
NsbI TGCGCA 1 cut(s) 1022
NsiI ATGCAT 1 cut(s) 79
NspI RCATGY 2 cut(s) 81, 809
PaeR7I CTCGAG 1 cut(s) 540
PagI TCATGA 1 cut(s) 468
PceI AGGCCT 1 cut(s) 1207
PctI GAATGC 2 cut(s) 625, 1527
PdmI GAANNNNTTC 1 cut(s) 1439
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 6 cut(s) 32, 35, 38, 372, 516, 955
PleI GAGTC 3 cut(s) 168, 857, 1620
PpsI GAGTC 3 cut(s) 168, 857, 1620
PshBI ATTAAT 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 541
PspN4I GGNNCC 2 cut(s) 693, 990
PspPI GGNCC 6 cut(s) 209, 605, 713, 989, 1142, 1196
PspXI VCTCGAGB 1 cut(s) 540
PsrI GAACNNNNNNTAC 4 cut(s) 52, 84, 312, 344
PsuI RGATCY 1 cut(s) 1009
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 7 cut(s) 64, 229, 316, 348, 1262, 1331, 1641
RsaNI GTAC 7 cut(s) 63, 228, 315, 347, 1261, 1330, 1640
RseI CAYNNNNRTG 2 cut(s) 76, 336
SacI GAGCTC 1 cut(s) 541
SalI GTCGAC 1 cut(s) 283
SaqAI TTAA 4 cut(s) 203, 420, 666, 1071
SatI GCNGC 6 cut(s) 31, 34, 37, 371, 515, 954
Sau3AI GATC 6 cut(s) 246, 958, 1009, 1396, 1599, 1648
Sau96I GGNCC 6 cut(s) 209, 605, 713, 989, 1142, 1196
ScaI AGTACT 2 cut(s) 64, 316
SchI GAGTC 3 cut(s) 169, 857, 1620
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 541
SfaNI GCATC 2 cut(s) 574, 1264
SfcI CTRYAG 2 cut(s) 318, 823
Sfr274I CTCGAG 1 cut(s) 540
SinI GGWCC 2 cut(s) 605, 1196
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 2 cut(s) 76, 336
SmlI CTYRAG 2 cut(s) 277, 540
SmoI CTYRAG 2 cut(s) 277, 540
SpeI ACTAGT 1 cut(s) 503
SseBI AGGCCT 1 cut(s) 1207
SspI AATATT 2 cut(s) 913, 1028
SspMI CTAG 4 cut(s) 504, 518, 786, 1217
SstI GAGCTC 1 cut(s) 541
StuI AGGCCT 1 cut(s) 1207
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 3 cut(s) 1135, 1151, 1346
TaaI ACNGT 8 cut(s) 289, 311, 319, 663, 702, 827, 895, 1196
TaiI ACGT 1 cut(s) 270
TaqI TCGA 4 cut(s) 284, 541, 562, 1163
TaqII GACCGA 1 cut(s) 345
TatI WGTACW 4 cut(s) 62, 314, 346, 1639
TauI GCSGC 5 cut(s) 33, 36, 39, 373, 517
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 4 cut(s) 203, 420, 666, 1071
Tru9I TTAA 4 cut(s) 203, 420, 666, 1071
TscAI CASTG 3 cut(s) 705, 832, 1464
TseFI GTSAC 3 cut(s) 829, 860, 1118
TseI GCWGC 1 cut(s) 953
Tsp45I GTSAC 3 cut(s) 829, 860, 1118
TspDTI ATGAA 7 cut(s) 60, 175, 461, 465, 485, 695, 696
TspGWI ACGGA 1 cut(s) 1025
TspRI CASTG 3 cut(s) 705, 832, 1464
VpaK11BI GGWCC 2 cut(s) 605, 1196
VspI ATTAAT 1 cut(s) 420
XagI CCTNNNNNAGG 2 cut(s) 634, 1268
XapI RAATTY 3 cut(s) 166, 745, 1003
XceI RCATGY 2 cut(s) 81, 809
XhoI CTCGAG 1 cut(s) 540
XmiI GTMKAC 2 cut(s) 284, 500
XmnI GAANNNNTTC 1 cut(s) 1439
XspI CTAG 4 cut(s) 504, 518, 786, 1217
ZrmI AGTACT 2 cut(s) 64, 316
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.