Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004055

Physical Location & Seq

Genomic Coordinates Forward (+)

12480454 .. 12494919

Gene Structure 14466 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1094500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGGCGCCACCCCCTCTCTCGCGCGGATTGAAGAGAAAAGCCAAGAAGCAGCAAATTCAAAAGCAAGACGCAGAGCTCGAGCGCTTCGATTCGCTCCCATGGAACCCCTCTCTCCCCAACGACGACAACGCCTTCTCGCAAATGGTCGGCAACAACGAGCTCGAAGGAGGTTTCCTAATGCTTGAGGAGATTGACGAGGCCGCATATGGGTTTGAAATTCCTGAACCACAAGTAGCAAATAGGAATAGCAAGGAAGACAGTAAGCAAAGGGAAAAGTCCAAGAAGCGGAAGCGCAGCAGTGGAGTTGATGGGGGTGCTGGTGATGGGGTTGAGGCGGATGGCGAAAGGATTTTGGGCAAGGATGATAGCGAGCTAAATAAAAAGAAGAAAAAGAAACAGAAAAAGAAAAAGAAGCCGGACCAAGCTCAGAGAATTGAGAATGTTGATGGAAATGGTGACAATGAGTTTGAAGATAAAAGCAAGGATGAGGATGTGAATTCTAAGAAGAAGAGGAAGAGGAAGACGTTGACGAAGAAGAAGAACAACGCCAAGAAGACCCTGGAAAATGTGGAGAGTACAGTTGATGATGATAGCAAAGGAAAAACAGAAATTGACAAAGAGAGTTTTGATGAAGATGAGTATTATGCATGGCATGAGTTGAGACTCCATCATCTGATCATGAAATCAATATATAGGCTTGGGTTCAAGAACCCAACGCCAATACAAAAAGCTTGTATTCCTGCTGCTGCTCATCAAGGGAAGGACATTGTTGGTGCTGCTGAAACAGGGTCTGGAAAGACACTTGCTTTTGGTTTGCCCATTTTGCAACGGTTATTGGATGAAAAAGAAAAGGCTGTAAGGATGTTTGATGAAAAGGGGGAGGAAGCAGAGAAGTTTTCTCCAAAAGGTCTTTTACGGGCCCTTGTCATTACTCCCACAAGGGAACTTGCACTTCAGGTCTCTGATCATCTTAAGGCAGTTGCCAAGGATACTGATATTAGAGTGGTTCCAATAGTTGGAGGGATGTCAAAGGAAAAACAGGAGAGACTTCTAAAGGCTAGACCTGAGATTGTTGTTGGGACTCCAGGGAGATTATGGGAACTTATGTCTGGTGGAGAAAAGCATCTAGTTGAGTTACATTCGTTGTCTTTCTTTGTGCTGGACGAGGCTGATCGAATGATAGAAAATGGTCATTTTCATGAAATGCAGTCAATAATTGACATGCTTCCAGTGAGGAATACATCAACTGAATCCCATTCTGAAAATGCACAAAATTCTGTTGCAATGTCCAACTTCCAGAGGAAGAAAAGACAAACATTTGTTTTCTCTGCTACTATAGCACTTTCTCCTGATTTCCGTAAGAAGTTAAAGCGTAGTTCACAAAAAACCAACCAATCAATGTCTGATGGGGTGAATTCTATAGAAGCTCTAAGTGAGCGAGCAGGGATGAGAGACAATGTTTCCGTCATTGATCTGACAAATACATCCATTTTGGCAAATAAGCTTGTGGAGTCATTTATTGAATGCAGGGAAGAAGATAAAGACGCCTACCTGTATTATATTTTAAGTGTTCATGGACAAGGTCGCACAATTGTATTTTGTACATCAGTTGCAGCTTTGCGACACATATCATCACTCCTGTGCATTCTTGGCATCAATGTCTGGACACTTCATGCTCAGATGCAGCAGCGAGCTCGCTTGAAGGCAATTGATCGTTTTCGTGGATTTGAACATGGTTTACTTGTGGCTACTGATGTTGCAGCAAGAGGCCTGGATATTCCTGGTGTTCGAACTGTTGTTCACTACCAGCTTCCACATTCAGCAGAAGTTTATGTTCACAGAAGTGGTAGAACTGCTAGAGCTTCTGCTGATGGGTGCAGCATTGCACTAATTGCACCAAATGAGACCTCCAAATTTGCTTCTTTGTGCAAATCATTTTCTAAGGAAAGTTTTCAGCGATTTCCCATGGACAGCTCATACATGCCTGAGGTCATGAAACGGTTATCTCTTGCACGTCAAATAGACAAGATTATGCGGAAAGATTCCCAGGAGAAATCGAAGAAAAATTGGGTTGAAAGAAATGCAGAATTGATTGAATTGGTTGTGGACCATGATGACAGTGAGGAGGAAAGGGTGAAAAGTCATAAGCAAAAGAAAGCCAGCTCTGCGAGTTTAAAGAAGTTGCAGCAGGAGCTCAAGATGTTACTTTCGCGACCATTACAACCGAAGTCATTTTCACATCGTTATTTGACAGGGGCTGGTGTGTCACCTTTCCTGCTACATCAATTCGAAGAATTGGCTAAGCAAAAATTTGGTGATGGCAGTGACTCGGGAGATAAGAAAAGAAGCAAATTGGTAGTTATTGGTCAGGATTGCGTGGAACCACTCCAGGCACTTCGGAGTGCTGGGCATGAGGTACACATTGATGGGAAAGAGGTGGCTCAAAAGCGAAGAAACATGGTGACTCTGAGGAGGAAAAGAAAAGAGGAGAAAACACGTTTGAGAGATCAACGGAGAAAGCGGAAGAAACAGATGAAGGGAGGAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

850

Amino Acids

96.03

Weight (kDa)

9.46

Isoelectric Point (pI)

50.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	239 - 447	2.9e-40	DEAD/DEAH box helicase
Helicase_C	PF00271	514 - 621	2.4e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16840 AT3G16840
fragaria_vesca	FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910
malus_domestica	MD12G1094500.v1.1 MD14G1084300.v1.1
prunus_persica	Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1
pyrus_communis	pycom14g07400
rosa_chinensis	RchiOBHm_Chr3g0492441
rosa_laevigata	RLG00000022766
rosa_multiflora	Rmu_sc0003156.1_g000008 Rmu_sc0007709.1_g000003 Rmu_sc0017916.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392260
rosa_rugosa	Rorug03G0253900
rosa_samantha	Rh3AG304100 Rh3BG340000 Rh3CG337200 Rh3DG339200
rosa_wichuraiana	Rw3G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	4
AccB7I	CCANNNNNTGG	1 cut(s)	1016
AccII	CGCG	3 cut(s)	22, 24, 2212
AciI	CCGC	6 cut(s)	24, 201, 286, 335, 2035, 2521
AcsI	RAATTY	7 cut(s)	54, 216, 496, 1273, 1414, 1913, 2309
AcuI	CTGAAG	1 cut(s)	938
AcyI	GRCGYC	2 cut(s)	5, 1545
AfaI	GTAC	3 cut(s)	577, 1603, 2418
AfeI	AGCGCT	1 cut(s)	83
AfiI	CCNNNNNNNGG	3 cut(s)	207, 285, 1016
AflII	CTTAAG	1 cut(s)	971
AflIII	ACRYGT	1 cut(s)	2495
AjiI	CACGTC	1 cut(s)	2015
AjnI	CCWGG	6 cut(s)	558, 1084, 1770, 1780, 2046, 2388
AleI	CACNNNNGTG	2 cut(s)	1639, 1842
Alw21I	GWGCWC	4 cut(s)	78, 162, 1696, 2196
Alw26I	GTCTC	5 cut(s)	655, 964, 1039, 1446, 1898
AlwNI	CAGNNNCTG	2 cut(s)	791, 2258
Ama87I	CYCGRG	2 cut(s)	77, 2329
Aor51HI	AGCGCT	1 cut(s)	83
AoxI	GGCC	3 cut(s)	198, 918, 1768
ApaI	GGGCCC	1 cut(s)	922
ApoI	RAATTY	7 cut(s)	54, 216, 496, 1273, 1414, 1913, 2309
ArsI	GACNNNNNNTTYG	2 cut(s)	449, 481
Asp700I	GAANNNNTTC	1 cut(s)	1950
AspLEI	GCGC	4 cut(s)	7, 24, 84, 294
AspS9I	GGNCC	4 cut(s)	418, 918, 919, 2107
AsuHPI	GGTGA	7 cut(s)	332, 467, 1423, 2146, 2259, 2327, 2473
AsuII	TTCGAA	2 cut(s)	1789, 2289
AvaI	CYCGRG	2 cut(s)	77, 2329
AvaII	GGWCC	2 cut(s)	418, 2107
AxyI	CCTNAGG	1 cut(s)	1986
BaeGI	GKGCMC	1 cut(s)	922
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	5 cut(s)	78, 162, 922, 1696, 2196
BbsI	GAAGAC	3 cut(s)	261, 527, 560
Bbv12I	GWGCWC	4 cut(s)	78, 162, 1696, 2196
BccI	CCATC	9 cut(s)	302, 317, 332, 440, 675, 1400, 1865, 2312, 2420
BcgI	CGANNNNNNTGC	2 cut(s)	1676, 1710
BciT130I	CCWGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
BciVI	GTATCC	1 cut(s)	982
BclI	TGATCA	2 cut(s)	675, 964
BcoDI	GTCTC	5 cut(s)	655, 964, 1039, 1446, 1898
BfaI	CTAG	3 cut(s)	1059, 1127, 1857
BfmI	CTRYAG	2 cut(s)	1335, 1419
BfoI	RGCGCY	2 cut(s)	8, 85
BfrI	CTTAAG	1 cut(s)	971
BfuI	GTATCC	1 cut(s)	982
BlpI	GCTNAGC	1 cut(s)	2301
Bme1390I	CCNGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
Bme18I	GGWCC	2 cut(s)	418, 2107
BmeT110I	CYCGRG	2 cut(s)	77, 2329
BmgBI	CACGTC	1 cut(s)	2015
BmgT120I	GGNCC	4 cut(s)	418, 918, 919, 2107
BmiI	GGNNCC	5 cut(s)	6, 104, 920, 1008, 2382
BmrFI	CCNGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
BmsI	GCATC	3 cut(s)	1132, 1662, 1671
BpiI	GAAGAC	3 cut(s)	261, 527, 560
BpmI	CTGGAG	2 cut(s)	1068, 2372
Bpu1102I	GCTNAGC	1 cut(s)	2301
Bpu14I	TTCGAA	2 cut(s)	1789, 2289
BpuEI	CTTGAG	2 cut(s)	203, 2180
BsaHI	GRCGYC	2 cut(s)	5, 1545
BsaI	GGTCTC	2 cut(s)	964, 1898
BsaJI	CCNNGG	6 cut(s)	98, 558, 984, 1085, 1965, 2046
Bsc4I	CCNNNNNNNGG	3 cut(s)	207, 285, 1016
Bse1I	ACTGG	1 cut(s)	1229
Bse21I	CCTNAGG	1 cut(s)	1986
Bse3DI	GCAATG	2 cut(s)	1290, 1881
BseBI	CCWGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
BseDI	CCNNGG	6 cut(s)	98, 558, 984, 1085, 1965, 2046
BseGI	GGATG	9 cut(s)	343, 367, 490, 496, 844, 867, 1029, 1452, 1484
BseLI	CCNNNNNNNGG	3 cut(s)	207, 285, 1016
BseMI	GCAATG	2 cut(s)	1290, 1881
BseMII	CTCAG	5 cut(s)	440, 1056, 1691, 1977, 2459
BseNI	ACTGG	1 cut(s)	1229
BseRI	GAGGAG	4 cut(s)	200, 2138, 2485, 2501
BseSI	GKGCMC	1 cut(s)	922
BseYI	CCCAGC	1 cut(s)	2405
BsgI	GTGCAG	1 cut(s)	1897
Bsh1236I	CGCG	3 cut(s)	22, 24, 2212
BshFI	GGCC	3 cut(s)	200, 920, 1770
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	4 cut(s)	78, 162, 1696, 2196
BsiHKCI	CYCGRG	2 cut(s)	77, 2329
BsiSI	CCGG	1 cut(s)	416
BslFI	GGGAC	1 cut(s)	1093
BslI	CCNNNNNNNGG	3 cut(s)	207, 285, 1016
BsmAI	GTCTC	5 cut(s)	655, 964, 1039, 1446, 1898
BsmFI	GGGAC	1 cut(s)	1093
BsmI	GAATGC	2 cut(s)	1529, 1644
BsnI	GGCC	3 cut(s)	200, 920, 1770
Bso31I	GGTCTC	2 cut(s)	964, 1898
BsoBI	CYCGRG	2 cut(s)	77, 2329
Bsp119I	TTCGAA	2 cut(s)	1789, 2289
Bsp120I	GGGCCC	1 cut(s)	918
Bsp1286I	GDGCHC	5 cut(s)	78, 162, 922, 1696, 2196
Bsp1407I	TGTACA	1 cut(s)	1601
Bsp143I	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
Bsp1720I	GCTNAGC	1 cut(s)	2301
Bsp19I	CCATGG	2 cut(s)	98, 1965
Bsp68I	TCGCGA	1 cut(s)	2212
BspACI	CCGC	6 cut(s)	24, 201, 286, 335, 2035, 2521
BspANI	GGCC	3 cut(s)	200, 920, 1770
BspCNI	CTCAG	5 cut(s)	439, 1057, 1690, 1978, 2460
BspFNI	CGCG	3 cut(s)	22, 24, 2212
BspHI	TCATGA	3 cut(s)	678, 1198, 1992
BspLI	GGNNCC	5 cut(s)	6, 104, 920, 1008, 2382
BspT104I	TTCGAA	2 cut(s)	1789, 2289
BspT107I	GGYRCC	1 cut(s)	4
BspTI	CTTAAG	1 cut(s)	971
BspTNI	GGTCTC	2 cut(s)	964, 1898
BsrDI	GCAATG	2 cut(s)	1290, 1881
BsrGI	TGTACA	1 cut(s)	1601
BsrI	ACTGG	1 cut(s)	1229
BssECI	CCNNGG	6 cut(s)	98, 558, 984, 1085, 1965, 2046
BssMI	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
BssNI	GRCGYC	2 cut(s)	5, 1545
BssT1I	CCWWGG	3 cut(s)	98, 984, 1965
Bst2UI	CCWGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
Bst4CI	ACNGT	6 cut(s)	260, 580, 831, 1795, 2001, 2120
Bst6I	CTCTTC	3 cut(s)	26, 503, 509
BstACI	GRCGYC	2 cut(s)	5, 1545
BstAFI	CTTAAG	1 cut(s)	971
BstAPI	GCANNNNNTGC	1 cut(s)	1892
BstAUI	TGTACA	1 cut(s)	1601
BstBI	TTCGAA	2 cut(s)	1789, 2289
BstC8I	GCNNGC	5 cut(s)	371, 1440, 1692, 1696, 2161
BstDEI	CTNAG	9 cut(s)	426, 501, 1065, 1430, 1677, 1941, 1986, 2301, 2468
BstDSI	CCRYGG	2 cut(s)	98, 1965
BstF5I	GGATG	9 cut(s)	343, 367, 490, 496, 844, 867, 1029, 1452, 1484
BstFNI	CGCG	3 cut(s)	22, 24, 2212
BstH2I	RGCGCY	2 cut(s)	8, 85
BstHHI	GCGC	4 cut(s)	7, 24, 84, 294
BstKTI	GATC	6 cut(s)	678, 967, 1174, 1474, 1714, 2509
BstMAI	GTCTC	5 cut(s)	655, 964, 1039, 1446, 1898
BstMBI	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
BstMWI	GCNNNNNNNGC	6 cut(s)	823, 1337, 1650, 1892, 2165, 2191
BstNI	CCWGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
BstNSI	RCATGY	2 cut(s)	1225, 1984
BstSCI	CCNGG	6 cut(s)	558, 1084, 1770, 1780, 2046, 2388
BstSFI	CTRYAG	2 cut(s)	1335, 1419
BstSLI	GKGCMC	1 cut(s)	922
BstUI	CGCG	3 cut(s)	22, 24, 2212
BstV2I	GAAGAC	3 cut(s)	261, 527, 560
Bsu36I	CCTNAGG	1 cut(s)	1986
BsuI	GTATCC	1 cut(s)	982
BsuRI	GGCC	3 cut(s)	200, 920, 1770
BtgI	CCRYGG	2 cut(s)	98, 1965
BtrI	CACGTC	1 cut(s)	2015
BtsCI	GGATG	9 cut(s)	343, 367, 490, 496, 844, 867, 1029, 1452, 1484
BtsI	GCAGTG	2 cut(s)	304, 2329
BtsIMutI	CAGTG	4 cut(s)	304, 1236, 2125, 2329
BtuMI	TCGCGA	1 cut(s)	2212
Cac8I	GCNNGC	5 cut(s)	371, 1440, 1692, 1696, 2161
CaiI	CAGNNNCTG	2 cut(s)	791, 2258
CciI	TCATGA	3 cut(s)	678, 1198, 1992
CfoI	GCGC	4 cut(s)	7, 24, 84, 294
Cfr13I	GGNCC	4 cut(s)	418, 918, 919, 2107
CseI	GACGC	2 cut(s)	77, 1553
Csp6I	GTAC	3 cut(s)	576, 1602, 2417
CviQI	GTAC	3 cut(s)	576, 1602, 2417
DdeI	CTNAG	9 cut(s)	426, 501, 1065, 1430, 1677, 1941, 1986, 2301, 2468
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	6 cut(s)	677, 966, 1173, 1473, 1713, 2508
DpnII	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
DraI	TTTAAA	1 cut(s)	2175
Eam1104I	CTCTTC	3 cut(s)	26, 503, 509
EarI	CTCTTC	3 cut(s)	26, 503, 509
EciI	GGCGGA	1 cut(s)	350
Ecl136II	GAGCTC	4 cut(s)	76, 160, 1694, 2194
Eco130I	CCWWGG	3 cut(s)	98, 984, 1965
Eco147I	AGGCCT	1 cut(s)	1770
Eco24I	GRGCYC	5 cut(s)	78, 162, 922, 1696, 2196
Eco31I	GGTCTC	2 cut(s)	964, 1898
Eco47I	GGWCC	2 cut(s)	418, 2107
Eco47III	AGCGCT	1 cut(s)	83
Eco53kI	GAGCTC	4 cut(s)	76, 160, 1694, 2194
Eco57I	CTGAAG	1 cut(s)	938
Eco81I	CCTNAGG	1 cut(s)	1986
Eco88I	CYCGRG	2 cut(s)	77, 2329
EcoICRI	GAGCTC	4 cut(s)	76, 160, 1694, 2194
EcoO109I	RGGNCCY	1 cut(s)	919
EcoRI	GAATTC	2 cut(s)	496, 1414
EcoRII	CCWGG	6 cut(s)	558, 1084, 1770, 1780, 2046, 2388
EcoT14I	CCWWGG	3 cut(s)	98, 984, 1965
EcoT22I	ATGCAT	1 cut(s)	649
EcoT38I	GRGCYC	5 cut(s)	78, 162, 922, 1696, 2196
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	3 cut(s)	98, 984, 1965
FaqI	GGGAC	1 cut(s)	1093
FauNDI	CATATG	1 cut(s)	205
FbaI	TGATCA	2 cut(s)	675, 964
FokI	GGATG	9 cut(s)	350, 374, 497, 503, 851, 874, 1036, 1459, 1471
FriOI	GRGCYC	5 cut(s)	78, 162, 922, 1696, 2196
FspBI	CTAG	3 cut(s)	1059, 1127, 1857
GlaI	GCGC	4 cut(s)	6, 23, 83, 293
GsaI	CCCAGC	1 cut(s)	2409
GsuI	CTGGAG	2 cut(s)	1068, 2372
HaeII	RGCGCY	2 cut(s)	8, 85
HaeIII	GGCC	3 cut(s)	200, 920, 1770
HapII	CCGG	1 cut(s)	416
HgaI	GACGC	2 cut(s)	77, 1553
HhaI	GCGC	4 cut(s)	7, 24, 84, 294
Hin1I	GRCGYC	2 cut(s)	5, 1545
Hin6I	GCGC	4 cut(s)	5, 22, 82, 292
HinP1I	GCGC	4 cut(s)	5, 22, 82, 292
HincII	GTYRAC	1 cut(s)	528
HindII	GTYRAC	1 cut(s)	528
HindIII	AAGCTT	2 cut(s)	729, 1502
HinfI	GANTC	8 cut(s)	89, 663, 1081, 1250, 1511, 2042, 2327, 2464
HpaII	CCGG	1 cut(s)	416
HphI	GGTGA	7 cut(s)	332, 467, 1423, 2146, 2259, 2327, 2473
Hpy166II	GTNNAC	7 cut(s)	528, 1379, 1739, 1801, 1837, 2107, 2419
Hpy188I	TCNGA	9 cut(s)	429, 675, 964, 1261, 1405, 1476, 1680, 2400, 2469
Hpy8I	GTNNAC	7 cut(s)	528, 1379, 1739, 1801, 1837, 2107, 2419
Hpy99I	CGWCG	1 cut(s)	125
HpyAV	CCTTC	5 cut(s)	142, 158, 754, 1696, 2530
HpyCH4III	ACNGT	6 cut(s)	260, 580, 831, 1795, 2001, 2120
HpyCH4IV	ACGT	3 cut(s)	524, 2014, 2497
HpyF10VI	GCNNNNNNNGC	6 cut(s)	823, 1337, 1650, 1892, 2165, 2191
HpyF3I	CTNAG	9 cut(s)	426, 501, 1065, 1430, 1677, 1941, 1986, 2301, 2468
HpySE526I	ACGT	3 cut(s)	524, 2014, 2497
Hsp92I	GRCGYC	2 cut(s)	5, 1545
HspAI	GCGC	4 cut(s)	5, 22, 82, 292
KasI	GGCGCC	1 cut(s)	4
Ksp22I	TGATCA	2 cut(s)	675, 964
Kzo9I	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
LmnI	GCTCC	2 cut(s)	99, 2191
LweI	GCATC	3 cut(s)	1132, 1662, 1671
MaeI	CTAG	3 cut(s)	1059, 1127, 1857
MaeII	ACGT	3 cut(s)	524, 2014, 2497
MaeIII	GTNAC	6 cut(s)	455, 1134, 2202, 2265, 2324, 2461
MalI	GATC	6 cut(s)	677, 966, 1173, 1473, 1713, 2508
MboI	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
MfeI	CAATTG	2 cut(s)	1590, 1707
MhlI	GDGCHC	5 cut(s)	78, 162, 922, 1696, 2196
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	657, 1075, 1520, 2321, 2458
MmeI	TCCRAC	2 cut(s)	997, 1314
Mph1103I	ATGCAT	1 cut(s)	649
MroXI	GAANNNNTTC	1 cut(s)	1950
MseI	TTAA	4 cut(s)	972, 1367, 1565, 2174
MslI	CAYNNNNRTG	4 cut(s)	1197, 1639, 1842, 2424
MspCI	CTTAAG	1 cut(s)	971
MspI	CCGG	1 cut(s)	416
MspR9I	CCNGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
MunI	CAATTG	2 cut(s)	1590, 1707
Mva1269I	GAATGC	2 cut(s)	1529, 1644
MvaI	CCWGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
MvnI	CGCG	3 cut(s)	22, 24, 2212
MwoI	GCNNNNNNNGC	6 cut(s)	823, 1337, 1650, 1892, 2165, 2191
NarI	GGCGCC	1 cut(s)	5
NcoI	CCATGG	2 cut(s)	98, 1965
NdeI	CATATG	1 cut(s)	205
NdeII	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
NlaIV	GGNNCC	5 cut(s)	6, 104, 920, 1008, 2382
NmuCI	GTSAC	4 cut(s)	455, 2265, 2324, 2461
NruI	TCGCGA	1 cut(s)	2212
NsiI	ATGCAT	1 cut(s)	649
NspI	RCATGY	2 cut(s)	1225, 1984
NspV	TTCGAA	2 cut(s)	1789, 2289
OliI	CACNNNNGTG	2 cut(s)	1639, 1842
PaeR7I	CTCGAG	1 cut(s)	77
PagI	TCATGA	3 cut(s)	678, 1198, 1992
PceI	AGGCCT	1 cut(s)	1770
PcsI	WCGNNNNNNNCGW	3 cut(s)	75, 84, 153
PctI	GAATGC	2 cut(s)	1529, 1644
PdmI	GAANNNNTTC	1 cut(s)	1950
PfeI	GAWTC	3 cut(s)	89, 1250, 2042
PflFI	GACNNNGTC	1 cut(s)	1581
PflMI	CCANNNNNTGG	1 cut(s)	1016
PleI	GAGTC	5 cut(s)	657, 1075, 1519, 2321, 2458
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	5 cut(s)	657, 1075, 1519, 2321, 2458
Psp124BI	GAGCTC	4 cut(s)	78, 162, 1696, 2196
Psp6I	CCWGG	6 cut(s)	558, 1084, 1770, 1780, 2046, 2388
PspFI	CCCAGC	1 cut(s)	2405
PspGI	CCWGG	6 cut(s)	558, 1084, 1770, 1780, 2046, 2388
PspN4I	GGNNCC	5 cut(s)	6, 104, 920, 1008, 2382
PspOMI	GGGCCC	1 cut(s)	918
PspPI	GGNCC	4 cut(s)	418, 918, 919, 2107
PspXI	VCTCGAGB	1 cut(s)	77
PsrI	GAACNNNNNNTAC	2 cut(s)	1722, 1754
PstNI	CAGNNNCTG	2 cut(s)	791, 2258
PsyI	GACNNNGTC	1 cut(s)	1581
RruI	TCGCGA	1 cut(s)	2212
RsaI	GTAC	3 cut(s)	577, 1603, 2418
RsaNI	GTAC	3 cut(s)	576, 1602, 2417
RseI	CAYNNNNRTG	4 cut(s)	1197, 1639, 1842, 2424
SacI	GAGCTC	4 cut(s)	78, 162, 1696, 2196
SaqAI	TTAA	4 cut(s)	972, 1367, 1565, 2174
Sau3AI	GATC	6 cut(s)	675, 964, 1171, 1471, 1711, 2506
Sau96I	GGNCC	4 cut(s)	418, 918, 919, 2107
SchI	GAGTC	5 cut(s)	657, 1075, 1520, 2321, 2458
ScrFI	CCNGG	6 cut(s)	560, 1086, 1772, 1782, 2048, 2390
SduI	GDGCHC	5 cut(s)	78, 162, 922, 1696, 2196
SfaNI	GCATC	3 cut(s)	1132, 1662, 1671
SfcI	CTRYAG	2 cut(s)	1335, 1419
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	77
SfuI	TTCGAA	2 cut(s)	1789, 2289
SinI	GGWCC	2 cut(s)	418, 2107
SlaI	CTCGAG	1 cut(s)	77
SmiMI	CAYNNNNRTG	4 cut(s)	1197, 1639, 1842, 2424
SmlI	CTYRAG	4 cut(s)	77, 182, 971, 2195
SmoI	CTYRAG	4 cut(s)	77, 182, 971, 2195
SseBI	AGGCCT	1 cut(s)	1770
SsiI	CCGC	6 cut(s)	24, 201, 286, 335, 2035, 2521
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	3 cut(s)	1059, 1127, 1857
SstI	GAGCTC	4 cut(s)	78, 162, 1696, 2196
StuI	AGGCCT	1 cut(s)	1770
StyD4I	CCNGG	6 cut(s)	558, 1084, 1770, 1780, 2046, 2388
StyI	CCWWGG	3 cut(s)	98, 984, 1965
TaaI	ACNGT	6 cut(s)	260, 580, 831, 1795, 2001, 2120
TaiI	ACGT	3 cut(s)	527, 2017, 2500
TaqI	TCGA	7 cut(s)	78, 87, 162, 1174, 1789, 2057, 2289
TatI	WGTACW	2 cut(s)	575, 1601
TauI	GCSGC	1 cut(s)	203
TfiI	GAWTC	3 cut(s)	89, 1250, 2042
Tru1I	TTAA	4 cut(s)	972, 1367, 1565, 2174
Tru9I	TTAA	4 cut(s)	972, 1367, 1565, 2174
TscAI	CASTG	4 cut(s)	304, 1236, 2125, 2329
TseFI	GTSAC	4 cut(s)	455, 2265, 2324, 2461
Tsp45I	GTSAC	4 cut(s)	455, 2265, 2324, 2461
TspGWI	ACGGA	3 cut(s)	1346, 1453, 2527
TspRI	CASTG	4 cut(s)	304, 1236, 2125, 2329
Tth111I	GACNNNGTC	1 cut(s)	1581
Van91I	CCANNNNNTGG	1 cut(s)	1016
Vha464I	CTTAAG	1 cut(s)	971
VpaK11BI	GGWCC	2 cut(s)	418, 2107
XapI	RAATTY	7 cut(s)	54, 216, 496, 1273, 1414, 1913, 2309
XceI	RCATGY	2 cut(s)	1225, 1984
XcmI	CCANNNNNNNNNTGG	2 cut(s)	556, 1092
XhoI	CTCGAG	1 cut(s)	77
XmnI	GAANNNNTTC	1 cut(s)	1950
XspI	CTAG	3 cut(s)	1059, 1127, 1857
Zsp2I	ATGCAT	1 cut(s)	649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.