Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004055

Physical Location & Seq

Genomic Coordinates Reverse (-)

10216293 .. 10236895

Gene Structure 20603 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1084300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2595 bp

ATGGCGCCACCACCTCTCTCCCGCGGATTGAAGAGAAAAGCCAAGAAGCAGCAAATTCAACAGCAAGACGCAGAGCTCGAGCGCTTCGATTCGCTACCATGGAACCCCTCTCTCCCGAACGACGACAACGCCTTCACTCAACTGGTCGGCAACGACGAGCTCGAAGGAGGTTTCCTAATGCTTGAGGAGATTGATGAGGCCGCATATGGATTTCAAATTCCTGAACCACAAGTAGCAAATAGGAATAGCAAGGAAGACTGTAAGCAAAGTGAAAAGTCCAAGAAGCGGAAGCGCAGCAGCGGAGTTAATGGGGGTGCTGGCGATGGGGTTGAGGTGGATGGCGAAGAGAGATTGGGCAAGGATGATAACGAGCTAAATAAAAAGAAGAAAAAGAAACAGAAAAAGAAAAAGAAGCCGGACGAAGCTCAGGGAATTGAGAATGTTGATGGAAATGGTGACAATGAGGTTGAAGACAAAAGTAAGGATGAGGATGTGAAATCTAAGAAGAACAGGAAGAAGAAGAGCAAGGCCAAGAAGACCCTGGAAAATGTGGAGAGTACAGTTGATGACGATAGCAAAGGAAAAAAAGAAATTGACGAAGAGATTTTCGATGAAGATGAGTATTATGCATGGCATGAGTTGAGACTCCATCATCTGATCATGAAATCAATATATAGGCTTGGGTTCAAGAACCCAACGCCAATACAGAAAGCTTGTATTCCTGCTGCTGCTCATCAAGGGAAGGATGTTGTTGGTGCTGCCGAAACAGGGTCTGGAAAGACACTTGCTTTTGGTTTGCCCATTTTGCAACGCTTATTGGATGAAAAGGAAAAGGCTGTAAAGATGTTTGATGAAAACGGGGTGGAAGCAGAGAAGTTTGCTCCAAAAGGTCTTCTACGGGCCCTTATCATTACTCCCACAAGAGAACTTGCACTTCAGGTCTCTGATCATCTAAAGGCAGTTGCCAAGGATACTGATATTAGAGTGGTTCCAATAGTTGGAGGGATGTCAATGGAAAAGCAGGAGAGACTTCTAAAAGGTAGACCTGAGATTGTTGTTGGGACTCCGGGGAGATTATGGGAACTTATGTCTGGTGGAGAAAGGCATCTCGTCGAGTTACATTCATTGTCTTTTTTTGTGCTGGACGAGGCTGATCGAATGATAGAAAATGGTCATTTTCATGAAATGCAGTCAATAATTGACATGCTTCCAGTGAGGAATACATCAACTGAATCTCATTCTGAAAATGCACAAAATTCTGTTGCGATCTCCAACTTCCAGAGGAAGAAAAGACAAACATTTGTTTTCTCTGCTACTATAGCACTTTCTCCTGATTTCCGTAAGAAGTTAAAGCGTAGTTCACAAAAACCCAACCAATCAATGTCTGATGGGGTGAATTCTATAGAAGCTCTAAGTGAGCGAGCAGGGATGAGAGACAATGTTGCCATCATTGATCTGACAAATACATCCATTTTGGCAAATAAGCTTGTGGAGTCATTCATTGAATGCAAGGAAGAAGATAAAGACGCCTACCTGTATTATATTTTAAGTGTTCATGGACAAGGTCGCACAATCGTATTTTGTACATCAGTTGCAGCTTTGCGACACATATCATCACTCCTGCGCATTCTTGGCATCAATGTCTGGACACTGCATGCTCAGATGCAGCAGCGAGCTCGCTTGAAGGCAATTGATCGTTTTCGTGGAAATGAACATGGTTTACTTGTGGCCACTGATGTTGCAGCAAGAGGCCTGGATATTCCTGGTGTTCGAACTGTTGTTCACTACCAGCTCCCACATTCAGCAGAAGTTTATGTTCACAGAAGTGGTAGAACTGCTAGGGCTTCTGCTGATGGGTGCAGCATTGCACTAATTGCACCAAATGAGACCTCCAAATTTGCTTCATTGTGCAAATCATTTTCCAAGGAAAGTTTTCAGCGATTTCCCATGGACAGTGCATACTTGCCTGAGGTCATGAAACGGTTATCTCTTGCACGTCAAATAGACAAGATTATGCAGAAAGATTCCCAGGAGAAATCAAAGAAAAATTGGTTCGAAAGAAATGCAGCATTGATTGAATTAGTTGTGGAGTATGATGACAGTGAGGAGGAAAGGGTGAAAAGTCATAAGCAAAAGAAAGCCAGCTCTGCGAGTTTAAAGAAGTTGCAGCAGGAGCTCAAGATGTTACTTTCGTGCCCATTACAACCGAAGTCATTTTCACATCGTTATTTGACAGGGGCTGGTGTTTCACCCTTCCTGCTACATCAATTCGAAGAATTGGCTAAGCAAAAATTTGGTGATAGCAGTGACTTGGGAGACAAGAAAAGAAGCAAATTGGTAGTTATTGGTCAGGATTGCGTGGAACCACTCCAGGCACTTCGGAGTGCTGGGCATAAGGTACACATTGATGGGAAAGAGGTGGCTCAAAAGAGAAGAAACATGGTGACTTTGAGGAGGAAAAGAAAAGAGGAGAAAACACGTACATTCTTCTTCCTTTTTCTTTCTGTGTGTTTTTGGGTGGAGGATGTGAGGGGTGGTTGGTGTTTGAGAGATCAACGGAGAAAGCGGAAGAAACAGATGAATGAAGGAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

865

Amino Acids

97.67

Weight (kDa)

9.19

Isoelectric Point (pI)

49.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	233 - 441	1.6e-40	DEAD/DEAH box helicase
Helicase_C	PF00271	508 - 615	1.7e-27	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16840 AT3G16840
fragaria_vesca	FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910
malus_domestica	MD12G1094500.v1.1 MD14G1084300.v1.1
prunus_persica	Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1
pyrus_communis	pycom14g07400
rosa_chinensis	RchiOBHm_Chr3g0492441
rosa_laevigata	RLG00000022766
rosa_multiflora	Rmu_sc0003156.1_g000008 Rmu_sc0007709.1_g000003 Rmu_sc0017916.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392260
rosa_rugosa	Rorug03G0253900
rosa_samantha	Rh3AG304100 Rh3BG340000 Rh3CG337200 Rh3DG339200
rosa_wichuraiana	Rw3G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1625
AccB1I	GGYRCC	1 cut(s)	4
AccB7I	CCANNNNNTGG	1 cut(s)	998
AccI	GTMKAC	1 cut(s)	1042
AccII	CGCG	1 cut(s)	24
AciI	CCGC	6 cut(s)	22, 24, 201, 286, 300, 2566
AcoI	YGGCCR	1 cut(s)	1728
AcsI	RAATTY	6 cut(s)	54, 216, 1255, 1396, 1895, 2291
AcuI	CTGAAG	1 cut(s)	920
AcyI	GRCGYC	2 cut(s)	5, 1527
AfaI	GTAC	4 cut(s)	559, 1585, 2400, 2482
AfeI	AGCGCT	1 cut(s)	83
AfiI	CCNNNNNNNGG	3 cut(s)	285, 768, 998
AflIII	ACRYGT	1 cut(s)	2477
AgsI	TTSAA	8 cut(s)	31, 59, 215, 470, 688, 1505, 1684, 2078
AjiI	CACGTC	1 cut(s)	1997
AjnI	CCWGG	5 cut(s)	540, 1752, 1762, 2028, 2370
AleI	CACNNNNGTG	1 cut(s)	1824
Alw21I	GWGCWC	4 cut(s)	78, 162, 1678, 2178
Alw26I	GTCTC	6 cut(s)	637, 946, 1021, 1428, 1880, 2310
AlwNI	CAGNNNCTG	2 cut(s)	773, 2240
Ama87I	CYCGRG	1 cut(s)	77
Aor51HI	AGCGCT	1 cut(s)	83
AoxI	GGCC	5 cut(s)	198, 528, 900, 1728, 1750
ApaI	GGGCCC	1 cut(s)	904
ApoI	RAATTY	6 cut(s)	54, 216, 1255, 1396, 1895, 2291
Asp700I	GAANNNNTTC	1 cut(s)	1932
AspLEI	GCGC	4 cut(s)	7, 84, 294, 1626
AspS9I	GGNCC	2 cut(s)	900, 901
AsuC2I	CCSGG	1 cut(s)	1068
AsuHPI	GGTGA	6 cut(s)	467, 1405, 2128, 2241, 2309, 2455
AsuII	TTCGAA	3 cut(s)	1771, 2055, 2271
AvaI	CYCGRG	1 cut(s)	77
AxyI	CCTNAGG	1 cut(s)	1968
BaeGI	GKGCMC	2 cut(s)	904, 2198
BalI	TGGCCA	1 cut(s)	1730
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	5 cut(s)	78, 162, 904, 1678, 2178
BbsI	GAAGAC	4 cut(s)	261, 477, 542, 884
Bbv12I	GWGCWC	4 cut(s)	78, 162, 1678, 2178
BccI	CCATC	8 cut(s)	317, 332, 440, 657, 1382, 1454, 1847, 2402
BcgI	CGANNNNNNTGC	4 cut(s)	1658, 1692, 2045, 2079
BciT130I	CCWGG	5 cut(s)	542, 1754, 1764, 2030, 2372
BciVI	GTATCC	1 cut(s)	964
BclI	TGATCA	2 cut(s)	657, 946
BcnI	CCSGG	1 cut(s)	1068
BcoDI	GTCTC	6 cut(s)	637, 946, 1021, 1428, 1880, 2310
BfaI	CTAG	1 cut(s)	1839
BfmI	CTRYAG	2 cut(s)	1317, 1401
BfoI	RGCGCY	2 cut(s)	8, 85
BfuI	GTATCC	1 cut(s)	964
BlpI	GCTNAGC	1 cut(s)	2283
Bme1390I	CCNGG	6 cut(s)	542, 1068, 1754, 1764, 2030, 2372
BmeT110I	CYCGRG	1 cut(s)	77
BmgBI	CACGTC	1 cut(s)	1997
BmgT120I	GGNCC	2 cut(s)	900, 901
BmiI	GGNNCC	5 cut(s)	6, 104, 902, 990, 2364
BmrFI	CCNGG	6 cut(s)	542, 1068, 1754, 1764, 2030, 2372
BmsI	GCATC	3 cut(s)	1114, 1644, 1653
BpiI	GAAGAC	4 cut(s)	261, 477, 542, 884
BpmI	CTGGAG	1 cut(s)	2354
Bpu10I	CCTNAGC	1 cut(s)	426
Bpu1102I	GCTNAGC	1 cut(s)	2283
Bpu14I	TTCGAA	3 cut(s)	1771, 2055, 2271
BpuEI	CTTGAG	2 cut(s)	203, 2162
BpuMI	CCSGG	1 cut(s)	1068
BsaAI	YACGTR	1 cut(s)	2480
BsaHI	GRCGYC	2 cut(s)	5, 1527
BsaI	GGTCTC	2 cut(s)	946, 1880
BsaJI	CCNNGG	8 cut(s)	22, 98, 540, 966, 1067, 1923, 1947, 2028
Bsc4I	CCNNNNNNNGG	3 cut(s)	285, 768, 998
Bse1I	ACTGG	2 cut(s)	147, 1211
Bse21I	CCTNAGG	1 cut(s)	1968
Bse3DI	GCAATG	1 cut(s)	1863
BseBI	CCWGG	5 cut(s)	542, 1754, 1764, 2030, 2372
BseDI	CCNNGG	8 cut(s)	22, 98, 540, 966, 1067, 1923, 1947, 2028
BseLI	CCNNNNNNNGG	3 cut(s)	285, 768, 998
BseMI	GCAATG	1 cut(s)	1863
BseMII	CTCAG	4 cut(s)	440, 1038, 1673, 1959
BseNI	ACTGG	2 cut(s)	147, 1211
BseRI	GAGGAG	4 cut(s)	200, 2120, 2467, 2483
BseSI	GKGCMC	2 cut(s)	904, 2198
BseYI	CCCAGC	1 cut(s)	2387
BsgI	GTGCAG	1 cut(s)	1879
Bsh1236I	CGCG	1 cut(s)	24
BshFI	GGCC	5 cut(s)	200, 530, 902, 1730, 1752
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	4 cut(s)	78, 162, 1678, 2178
BsiHKCI	CYCGRG	1 cut(s)	77
BsiSI	CCGG	2 cut(s)	416, 1067
BslFI	GGGAC	1 cut(s)	1075
BslI	CCNNNNNNNGG	3 cut(s)	285, 768, 998
BsmAI	GTCTC	6 cut(s)	637, 946, 1021, 1428, 1880, 2310
BsmFI	GGGAC	1 cut(s)	1075
BsmI	GAATGC	2 cut(s)	1511, 1626
BsnI	GGCC	5 cut(s)	200, 530, 902, 1730, 1752
Bso31I	GGTCTC	2 cut(s)	946, 1880
BsoBI	CYCGRG	1 cut(s)	77
Bsp119I	TTCGAA	3 cut(s)	1771, 2055, 2271
Bsp120I	GGGCCC	1 cut(s)	900
Bsp1286I	GDGCHC	6 cut(s)	78, 162, 904, 1678, 2178, 2198
Bsp1407I	TGTACA	1 cut(s)	1583
Bsp143I	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
Bsp1720I	GCTNAGC	1 cut(s)	2283
Bsp19I	CCATGG	2 cut(s)	98, 1947
BspACI	CCGC	6 cut(s)	22, 24, 201, 286, 300, 2566
BspANI	GGCC	5 cut(s)	200, 530, 902, 1730, 1752
BspCNI	CTCAG	4 cut(s)	439, 1039, 1672, 1960
BspFNI	CGCG	1 cut(s)	24
BspHI	TCATGA	3 cut(s)	660, 1180, 1974
BspLI	GGNNCC	5 cut(s)	6, 104, 902, 990, 2364
BspQI	GCTCTTC	1 cut(s)	515
BspT104I	TTCGAA	3 cut(s)	1771, 2055, 2271
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	2 cut(s)	946, 1880
BsrDI	GCAATG	1 cut(s)	1863
BsrGI	TGTACA	1 cut(s)	1583
BsrI	ACTGG	2 cut(s)	147, 1211
BssECI	CCNNGG	8 cut(s)	22, 98, 540, 966, 1067, 1923, 1947, 2028
BssMI	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
BssNI	GRCGYC	2 cut(s)	5, 1527
BssT1I	CCWWGG	4 cut(s)	98, 966, 1923, 1947
Bst2UI	CCWGG	5 cut(s)	542, 1754, 1764, 2030, 2372
Bst4CI	ACNGT	6 cut(s)	260, 562, 1777, 1955, 1983, 2102
Bst6I	CTCTTC	4 cut(s)	26, 339, 515, 594
BstACI	GRCGYC	2 cut(s)	5, 1527
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstAUI	TGTACA	1 cut(s)	1583
BstBAI	YACGTR	1 cut(s)	2480
BstBI	TTCGAA	3 cut(s)	1771, 2055, 2271
BstC8I	GCNNGC	6 cut(s)	319, 1422, 1656, 1674, 1678, 2143
BstDEI	CTNAG	7 cut(s)	426, 501, 1047, 1412, 1659, 1968, 2283
BstDSI	CCRYGG	3 cut(s)	22, 98, 1947
BstFNI	CGCG	1 cut(s)	24
BstH2I	RGCGCY	2 cut(s)	8, 85
BstHHI	GCGC	4 cut(s)	7, 84, 294, 1626
BstKTI	GATC	7 cut(s)	660, 949, 1156, 1269, 1456, 1696, 2554
BstMAI	GTCTC	6 cut(s)	637, 946, 1021, 1428, 1880, 2310
BstMBI	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
BstMWI	GCNNNNNNNGC	6 cut(s)	805, 1319, 1632, 1874, 2147, 2173
BstNI	CCWGG	5 cut(s)	542, 1754, 1764, 2030, 2372
BstNSI	RCATGY	2 cut(s)	1207, 1658
BstSCI	CCNGG	6 cut(s)	540, 1066, 1752, 1762, 2028, 2370
BstSFI	CTRYAG	2 cut(s)	1317, 1401
BstSLI	GKGCMC	2 cut(s)	904, 2198
BstUI	CGCG	1 cut(s)	24
BstV2I	GAAGAC	4 cut(s)	261, 477, 542, 884
Bsu36I	CCTNAGG	1 cut(s)	1968
BsuI	GTATCC	1 cut(s)	964
BsuRI	GGCC	5 cut(s)	200, 530, 902, 1730, 1752
BtgI	CCRYGG	3 cut(s)	22, 98, 1947
BtgZI	GCGATG	1 cut(s)	336
BtrI	CACGTC	1 cut(s)	1997
BtsI	GCAGTG	2 cut(s)	1649, 2311
BtsIMutI	CAGTG	6 cut(s)	1218, 1649, 1731, 1960, 2107, 2311
Cac8I	GCNNGC	6 cut(s)	319, 1422, 1656, 1674, 1678, 2143
CaiI	CAGNNNCTG	2 cut(s)	773, 2240
CciI	TCATGA	3 cut(s)	660, 1180, 1974
CfoI	GCGC	4 cut(s)	7, 84, 294, 1626
Cfr13I	GGNCC	2 cut(s)	900, 901
Cfr42I	CCGCGG	1 cut(s)	25
CseI	GACGC	2 cut(s)	77, 1535
Csp6I	GTAC	4 cut(s)	558, 1584, 2399, 2481
CspCI	CAANNNNNGTGG	2 cut(s)	1720, 1755
CviQI	GTAC	4 cut(s)	558, 1584, 2399, 2481
DdeI	CTNAG	7 cut(s)	426, 501, 1047, 1412, 1659, 1968, 2283
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	7 cut(s)	659, 948, 1155, 1268, 1455, 1695, 2553
DpnII	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
DraI	TTTAAA	1 cut(s)	2157
EaeI	YGGCCR	1 cut(s)	1728
Eam1104I	CTCTTC	4 cut(s)	26, 339, 515, 594
EarI	CTCTTC	4 cut(s)	26, 339, 515, 594
Ecl136II	GAGCTC	4 cut(s)	76, 160, 1676, 2176
Eco130I	CCWWGG	4 cut(s)	98, 966, 1923, 1947
Eco147I	AGGCCT	1 cut(s)	1752
Eco24I	GRGCYC	5 cut(s)	78, 162, 904, 1678, 2178
Eco31I	GGTCTC	2 cut(s)	946, 1880
Eco47III	AGCGCT	1 cut(s)	83
Eco53kI	GAGCTC	4 cut(s)	76, 160, 1676, 2176
Eco57I	CTGAAG	1 cut(s)	920
Eco81I	CCTNAGG	1 cut(s)	1968
Eco88I	CYCGRG	1 cut(s)	77
EcoICRI	GAGCTC	4 cut(s)	76, 160, 1676, 2176
EcoO109I	RGGNCCY	1 cut(s)	901
EcoRI	GAATTC	1 cut(s)	1396
EcoRII	CCWGG	5 cut(s)	540, 1752, 1762, 2028, 2370
EcoT14I	CCWWGG	4 cut(s)	98, 966, 1923, 1947
EcoT22I	ATGCAT	1 cut(s)	631
EcoT38I	GRGCYC	5 cut(s)	78, 162, 904, 1678, 2178
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	4 cut(s)	98, 966, 1923, 1947
FaqI	GGGAC	1 cut(s)	1075
FauI	CCCGC	1 cut(s)	29
FauNDI	CATATG	1 cut(s)	205
FbaI	TGATCA	2 cut(s)	657, 946
FblI	GTMKAC	1 cut(s)	1042
FriOI	GRGCYC	5 cut(s)	78, 162, 904, 1678, 2178
FspBI	CTAG	1 cut(s)	1839
FspI	TGCGCA	1 cut(s)	1625
GlaI	GCGC	4 cut(s)	6, 83, 293, 1625
GsaI	CCCAGC	1 cut(s)	2391
GsuI	CTGGAG	1 cut(s)	2354
HaeII	RGCGCY	2 cut(s)	8, 85
HaeIII	GGCC	5 cut(s)	200, 530, 902, 1730, 1752
HapII	CCGG	2 cut(s)	416, 1067
HgaI	GACGC	2 cut(s)	77, 1535
HhaI	GCGC	4 cut(s)	7, 84, 294, 1626
Hin1I	GRCGYC	2 cut(s)	5, 1527
Hin6I	GCGC	4 cut(s)	5, 82, 292, 1624
HinP1I	GCGC	4 cut(s)	5, 82, 292, 1624
HindIII	AAGCTT	2 cut(s)	711, 1484
HinfI	GANTC	6 cut(s)	89, 645, 1063, 1232, 1493, 2024
HpaII	CCGG	2 cut(s)	416, 1067
HphI	GGTGA	6 cut(s)	467, 1405, 2128, 2241, 2309, 2455
Hpy166II	GTNNAC	6 cut(s)	1043, 1361, 1721, 1783, 1819, 2401
Hpy188I	TCNGA	7 cut(s)	657, 946, 1243, 1387, 1458, 1662, 2382
Hpy8I	GTNNAC	6 cut(s)	1043, 1361, 1721, 1783, 1819, 2401
Hpy99I	CGWCG	3 cut(s)	125, 158, 1115
HpyAV	CCTTC	6 cut(s)	142, 158, 736, 1678, 2263, 2579
HpyCH4III	ACNGT	6 cut(s)	260, 562, 1777, 1955, 1983, 2102
HpyCH4IV	ACGT	2 cut(s)	1996, 2479
HpyF10VI	GCNNNNNNNGC	6 cut(s)	805, 1319, 1632, 1874, 2147, 2173
HpyF3I	CTNAG	7 cut(s)	426, 501, 1047, 1412, 1659, 1968, 2283
HpySE526I	ACGT	2 cut(s)	1996, 2479
Hsp92I	GRCGYC	2 cut(s)	5, 1527
HspAI	GCGC	4 cut(s)	5, 82, 292, 1624
KasI	GGCGCC	1 cut(s)	4
Ksp22I	TGATCA	2 cut(s)	657, 946
KspI	CCGCGG	1 cut(s)	25
Kzo9I	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
LguI	GCTCTTC	1 cut(s)	515
LmnI	GCTCC	3 cut(s)	886, 1797, 2173
LweI	GCATC	3 cut(s)	1114, 1644, 1653
MaeI	CTAG	1 cut(s)	1839
MaeII	ACGT	2 cut(s)	1996, 2479
MaeIII	GTNAC	5 cut(s)	455, 1116, 2184, 2306, 2443
MalI	GATC	7 cut(s)	659, 948, 1155, 1268, 1455, 1695, 2553
MboI	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
MfeI	CAATTG	1 cut(s)	1689
MhlI	GDGCHC	6 cut(s)	78, 162, 904, 1678, 2178, 2198
MlsI	TGGCCA	1 cut(s)	1730
MluNI	TGGCCA	1 cut(s)	1730
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	639, 1057, 1502
MmeI	TCCRAC	2 cut(s)	979, 1296
Mox20I	TGGCCA	1 cut(s)	1730
Mph1103I	ATGCAT	1 cut(s)	631
MroXI	GAANNNNTTC	1 cut(s)	1932
MscI	TGGCCA	1 cut(s)	1730
MseI	TTAA	4 cut(s)	306, 1349, 1547, 2156
MslI	CAYNNNNRTG	3 cut(s)	1179, 1824, 2406
Msp20I	TGGCCA	1 cut(s)	1730
MspA1I	CMGCKG	2 cut(s)	24, 300
MspI	CCGG	2 cut(s)	416, 1067
MspR9I	CCNGG	6 cut(s)	542, 1068, 1754, 1764, 2030, 2372
MunI	CAATTG	1 cut(s)	1689
Mva1269I	GAATGC	2 cut(s)	1511, 1626
MvaI	CCWGG	5 cut(s)	542, 1754, 1764, 2030, 2372
MvnI	CGCG	1 cut(s)	24
MwoI	GCNNNNNNNGC	6 cut(s)	805, 1319, 1632, 1874, 2147, 2173
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	1068
NcoI	CCATGG	2 cut(s)	98, 1947
NdeI	CATATG	1 cut(s)	205
NdeII	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
NlaIV	GGNNCC	5 cut(s)	6, 104, 902, 990, 2364
NmuCI	GTSAC	3 cut(s)	455, 2306, 2443
NsbI	TGCGCA	1 cut(s)	1625
NsiI	ATGCAT	1 cut(s)	631
NspI	RCATGY	2 cut(s)	1207, 1658
NspV	TTCGAA	3 cut(s)	1771, 2055, 2271
OliI	CACNNNNGTG	1 cut(s)	1824
PaeI	GCATGC	1 cut(s)	1658
PaeR7I	CTCGAG	1 cut(s)	77
PagI	TCATGA	3 cut(s)	660, 1180, 1974
PceI	AGGCCT	1 cut(s)	1752
PciSI	GCTCTTC	1 cut(s)	515
PcsI	WCGNNNNNNNCGW	4 cut(s)	75, 84, 153, 159
PctI	GAATGC	2 cut(s)	1511, 1626
PdmI	GAANNNNTTC	1 cut(s)	1932
PfeI	GAWTC	3 cut(s)	89, 1232, 2024
PflFI	GACNNNGTC	1 cut(s)	1563
PflMI	CCANNNNNTGG	1 cut(s)	998
PleI	GAGTC	3 cut(s)	639, 1057, 1501
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	3 cut(s)	639, 1057, 1501
Ppu21I	YACGTR	1 cut(s)	2480
Psp124BI	GAGCTC	4 cut(s)	78, 162, 1678, 2178
Psp6I	CCWGG	5 cut(s)	540, 1752, 1762, 2028, 2370
PspFI	CCCAGC	1 cut(s)	2387
PspGI	CCWGG	5 cut(s)	540, 1752, 1762, 2028, 2370
PspN4I	GGNNCC	5 cut(s)	6, 104, 902, 990, 2364
PspOMI	GGGCCC	1 cut(s)	900
PspPI	GGNCC	2 cut(s)	900, 901
PspXI	VCTCGAGB	1 cut(s)	77
PsrI	GAACNNNNNNTAC	2 cut(s)	1704, 1736
PstNI	CAGNNNCTG	2 cut(s)	773, 2240
PsyI	GACNNNGTC	1 cut(s)	1563
RsaI	GTAC	4 cut(s)	559, 1585, 2400, 2482
RsaNI	GTAC	4 cut(s)	558, 1584, 2399, 2481
RseI	CAYNNNNRTG	3 cut(s)	1179, 1824, 2406
SacI	GAGCTC	4 cut(s)	78, 162, 1678, 2178
SacII	CCGCGG	1 cut(s)	25
SapI	GCTCTTC	1 cut(s)	515
SaqAI	TTAA	4 cut(s)	306, 1349, 1547, 2156
Sau3AI	GATC	7 cut(s)	657, 946, 1153, 1266, 1453, 1693, 2551
Sau96I	GGNCC	2 cut(s)	900, 901
SchI	GAGTC	3 cut(s)	639, 1057, 1502
ScrFI	CCNGG	6 cut(s)	542, 1068, 1754, 1764, 2030, 2372
SduI	GDGCHC	6 cut(s)	78, 162, 904, 1678, 2178, 2198
SfaNI	GCATC	3 cut(s)	1114, 1644, 1653
SfcI	CTRYAG	2 cut(s)	1317, 1401
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	77
Sfr303I	CCGCGG	1 cut(s)	25
SfuI	TTCGAA	3 cut(s)	1771, 2055, 2271
SgrBI	CCGCGG	1 cut(s)	25
SlaI	CTCGAG	1 cut(s)	77
SmiMI	CAYNNNNRTG	3 cut(s)	1179, 1824, 2406
SmlI	CTYRAG	3 cut(s)	77, 182, 2177
SmoI	CTYRAG	3 cut(s)	77, 182, 2177
SphI	GCATGC	1 cut(s)	1658
SseBI	AGGCCT	1 cut(s)	1752
SsiI	CCGC	6 cut(s)	22, 24, 201, 286, 300, 2566
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	1 cut(s)	1839
SstI	GAGCTC	4 cut(s)	78, 162, 1678, 2178
StuI	AGGCCT	1 cut(s)	1752
StyD4I	CCNGG	6 cut(s)	540, 1066, 1752, 1762, 2028, 2370
StyI	CCWWGG	4 cut(s)	98, 966, 1923, 1947
TaaI	ACNGT	6 cut(s)	260, 562, 1777, 1955, 1983, 2102
TaiI	ACGT	2 cut(s)	1999, 2482
TaqI	TCGA	9 cut(s)	78, 87, 162, 609, 1113, 1156, 1771, 2055, 2271
TatI	WGTACW	2 cut(s)	557, 1583
TauI	GCSGC	1 cut(s)	203
TfiI	GAWTC	3 cut(s)	89, 1232, 2024
Tru1I	TTAA	4 cut(s)	306, 1349, 1547, 2156
Tru9I	TTAA	4 cut(s)	306, 1349, 1547, 2156
TscAI	CASTG	6 cut(s)	1218, 1656, 1738, 1960, 2107, 2311
TseFI	GTSAC	3 cut(s)	455, 2306, 2443
Tsp45I	GTSAC	3 cut(s)	455, 2306, 2443
TspGWI	ACGGA	2 cut(s)	1328, 2572
TspRI	CASTG	6 cut(s)	1218, 1656, 1738, 1960, 2107, 2311
Tth111I	GACNNNGTC	1 cut(s)	1563
Van91I	CCANNNNNTGG	1 cut(s)	998
XapI	RAATTY	6 cut(s)	54, 216, 1255, 1396, 1895, 2291
XceI	RCATGY	2 cut(s)	1207, 1658
XcmI	CCANNNNNNNNNTGG	1 cut(s)	538
XhoI	CTCGAG	1 cut(s)	77
XmiI	GTMKAC	1 cut(s)	1042
XmnI	GAANNNNTTC	1 cut(s)	1932
XspI	CTAG	1 cut(s)	1839
Zsp2I	ATGCAT	1 cut(s)	631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.