Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0004055

Physical Location & Seq

Genomic Coordinates Forward (+)

36413224 .. 36421403

Gene Structure 8180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG340000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGACGGCGACGCCGTTGCCACCGCCTCTGTCACGTGGAGCGACGAGGAAAGCGAAGAAGAAACAAATTCAACAGCAACACGCAGAGCTGGAGCGTTTGGATTCTCTCCCATGGAACCCCTCCTCTGTCCCTCTCCCTAATGACAATGATGATGCTGACCCTTTCTCCCATGTCAATGGCCTCGAAGGAGGTTGTCTTATGCTTGAAGAGATTGATGAGGCTGATTATGGCTTAGAAATTCCTGCACTAGAAGTAGAAAATAGAAATCGAAACGATAAGTCTAAGCCAACTAAAAAGTCCAAGAAACGAAAGCATGACCAACTTGATGCAAGTGATACCTTGGTAGAGGATGTTATTGCTGAAGAGAGATTGGACAAGGATGAGAAACACATGACTAAGAAGGAGAAAACGAAGAAGAAGAAGATGCTGGAGGAACCTCAGAAAATTGAGGACAGTGATGATAATGGTGGCAGTGAAGTTGAAGAGAAAGACAATGATGGGGATGTCGGTTCAAAGAAGAAAACGAAGAAGAAGAAGGCGGCCGAGGAAAGCCAGAAAAATGAGGAATCCACAGTTGATGAGAAGGATGAAGTAGAAGAGCAAGTTGATGAGGATGAATACTATGCATGGAATGAGTTGAGACTTCATCCCCTGATTATGAGATCGATATACAGGCTTGGGTTCAAAGAACCAACGGCCATCCAGAAAGCTTGTATTCCTGCTGCTGCTCATCAAGGGAAGGATGTTGTTGGTGCTGCGGAAACAGGATCTGGCAAAACGTTAGCTTTTGGTCTGCCGATTTTGCAACGCCTGTTGGACGAAAGAGAAAAGGCTGCGAAGATGTTTGAAGAAAAGGGAGAGGAAGCAGAAAAAGTCACTTCGAAAGGCTTTTTACGGGCCCTTGTTATTACTCCTACAAGAGAACTTTCACTTCAGGTCTCTGATCATCTTAAGGCAGTTGCTAAGGATACTGATATTAGAGTGGTTCCTATAGTTGGAGGCATGTCAACGGACAAGCAGGAGAGACTTCTTAGATCAAGACCTGAGATTGTTGTTGGAACCCCAGGGAGATTATGGGAACTTATGTCTGGAGGAGAAAAGCATCTAGTTGAGTTGCATTCATTGTCTTTCTTTGTGCTGGATGAGGCTGATCGAATGATAGAAAACGGCCATTTCCATGAATTGCAGTCTATTATTGACATGCTTCCTTCAAGGAATAGTTCTGCCGTACATTCCGGAAATGCACAAGATTCTGTTACCATGACCAACTTCCAGAGGAAGAAGAGACAAACATTTGTTTTATCTGCAACAATAGCACTTTCTGCTGATTTCCGGAAAAAGCTGAAGCGTAGTTCTCTAAAACCAAAGCAATCAACGTCTGATGGGGTGAATTCTATAGAAGCTCTCTCTGAGCGAGCAGGGATGAGAGACAATGTTGCCATCATTGATCTGACAAATACATCCATTTTGGCGAATAATCTCGAAGAGTCATTTATTGAATGCAGGGAAGAAGACAAAGATGCCTATTTGTATTATATATTAAGTGTTCATGGGCAAGGTCGCACAATCATATTCTGTACGTCCATTGCAGCTTTGCGACATATAACATCGCTCTTGCGCATCCTTGGCACAGATGTTTTGACACTTCATGCTCAGATGCATCAGCGAGCTCGTTTGAAGGCAATTGACCGTTTTAGGGGGAGTGAACATGGTAAACTTGTGGCTACTGATGTTGCAGCAAGAGGCCTGGATATTCCTGGTGTTCGAACAGTTATTCACTACCAGCTTCCCCATTCAGCAGAAGTTTATGTTCACAGAAGTGGTAGAACTGCTAGAGCCACTGCTGATGGGTGTAGCATTGCACTAATTGCACCAAATGAGACATCCAAATTTGCTTCTTTGTGCAAATCATTTTCTAAGGTAAGTTTTCAACGATTTCCTTTGGAGAACTCATACTTCCCAGAGGTTATGAGACGATTATCTCTTGCGCGCCAAATAGACAAGATTCTGCGGAAGGATTCCCAGGACAAAGCAAAGAAAAGCTGGTATGAAAGAAATGCAGAATTGGTTGAATTGGTTGTGGACAGTGATGAGGATGAAGAGGATAGAGTTAAAAATTATAAGCAAAAGAAAGCCAGCTCCATGAATTTAAAAAGCTTGCAGCAGGAACTGAAGATGTTGCTTTCACGTCCATTACAACCGAAGTCATTTTCACATCGTTACTTGACTGGGTCTGGTATCACACCTCTGGTGCAACATCAATTTGAAGAATTGGCTAAGCAAAAACTAGGCGATAGCAGCAACTCAGGAAAAAGAAGTAAATTGTTAGTTATTGGTCAGAATTGTGTGGAACCACTCCAAGCACTTAGGAGTTCTGGGCATGAGGTAGTATTAAGCTCTACCCTTTCCCTATGTTTTCAGTCTCAACTTCACACTTGTCAGCAAGCAACACCTAAAACAAGAAGAATGCATGAATCAAGATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

828

Amino Acids

93.05

Weight (kDa)

7.92

Isoelectric Point (pI)

48.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	232 - 439	4.5e-39	DEAD/DEAH box helicase
Helicase_C	PF00271	506 - 613	1.3e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16840 AT3G16840
fragaria_vesca	FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910
malus_domestica	MD12G1094500.v1.1 MD14G1084300.v1.1
prunus_persica	Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1
pyrus_communis	pycom14g07400
rosa_chinensis	RchiOBHm_Chr3g0492441
rosa_laevigata	RLG00000022766
rosa_multiflora	Rmu_sc0003156.1_g000008 Rmu_sc0007709.1_g000003 Rmu_sc0017916.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392260
rosa_rugosa	Rorug03G0253900
rosa_samantha	Rh3AG304100 Rh3BG340000 Rh3CG337200 Rh3DG339200
rosa_wichuraiana	Rw3G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2121
Acc16I	TGCGCA	1 cut(s)	1619
AccII	CGCG	1 cut(s)	1990
AccIII	TCCGGA	2 cut(s)	1235, 1332
AciI	CCGC	4 cut(s)	23, 539, 758, 2011
AclI	AACGTT	1 cut(s)	779
AclWI	GGATC	2 cut(s)	775, 2475
AcoI	YGGCCR	3 cut(s)	540, 696, 1168
AcsI	RAATTY	5 cut(s)	66, 237, 1390, 1889, 2146
AcuI	CTGAAG	4 cut(s)	381, 917, 1364, 2192
AcvI	CACGTG	1 cut(s)	35
AcyI	GRCGYC	1 cut(s)	11
AfaI	GTAC	2 cut(s)	1230, 1579
AfiI	CCNNNNNNNGG	2 cut(s)	995, 1696
AflII	CTTAAG	1 cut(s)	950
AjiI	CACGTC	1 cut(s)	2189
AjnI	CCWGG	4 cut(s)	1063, 1746, 1756, 2022
AleI	CACNNNNGTG	1 cut(s)	1818
Alw21I	GWGCWC	1 cut(s)	1672
Alw26I	GTCTC	8 cut(s)	634, 943, 1018, 1279, 1422, 1874, 1966, 2427
AlwI	GGATC	2 cut(s)	775, 2475
AlwNI	CAGNNNCTG	2 cut(s)	770, 2170
Aor13HI	TCCGGA	2 cut(s)	1235, 1332
AoxI	GGCC	6 cut(s)	178, 540, 696, 897, 1168, 1744
ApaI	GGGCCC	1 cut(s)	901
ApeKI	GCWGC	8 cut(s)	722, 725, 755, 833, 1589, 1736, 2161, 2298
ApoI	RAATTY	5 cut(s)	66, 237, 1390, 1889, 2146
AspLEI	GCGC	3 cut(s)	1620, 1990, 1992
AspS9I	GGNCC	2 cut(s)	897, 898
AsuHPI	GGTGA	1 cut(s)	1399
AsuII	TTCGAA	2 cut(s)	881, 1765
BaeGI	GKGCMC	1 cut(s)	901
BaeI	ACNNNNGTAYC	2 cut(s)	327, 360
BanII	GRGCYC	2 cut(s)	901, 1672
BbrPI	CACGTG	1 cut(s)	35
BbsI	GAAGAC	1 cut(s)	1518
Bbv12I	GWGCWC	1 cut(s)	1672
BbvI	GCAGC	8 cut(s)	709, 712, 742, 820, 1601, 1748, 2173, 2310
BccI	CCATC	5 cut(s)	491, 707, 1376, 1448, 1841
BceAI	ACGGC	4 cut(s)	21, 711, 1183, 1211
BcgI	CGANNNNNNTGC	1 cut(s)	32
BciT130I	CCWGG	4 cut(s)	1065, 1748, 1758, 2024
BciVI	GTATCC	1 cut(s)	961
BclI	TGATCA	1 cut(s)	943
BcoDI	GTCTC	8 cut(s)	634, 943, 1018, 1279, 1422, 1874, 1966, 2427
BfaI	CTAG	4 cut(s)	248, 1106, 1833, 2288
BfmI	CTRYAG	2 cut(s)	990, 1395
BfrI	CTTAAG	1 cut(s)	950
BfuI	GTATCC	1 cut(s)	961
BisI	GCNGC	9 cut(s)	540, 723, 726, 756, 834, 1590, 1737, 2162, 2299
BlpI	GCTNAGC	1 cut(s)	2277
BlsI	GCNGC	9 cut(s)	541, 724, 727, 757, 835, 1591, 1738, 2163, 2300
Bme1390I	CCNGG	4 cut(s)	1065, 1748, 1758, 2024
BmgBI	CACGTC	1 cut(s)	2189
BmgT120I	GGNCC	2 cut(s)	897, 898
BmiI	GGNNCC	6 cut(s)	116, 435, 899, 987, 1060, 2352
BmrFI	CCNGG	4 cut(s)	1065, 1748, 1758, 2024
BmrI	ACTGGG	1 cut(s)	2238
BmsI	GCATC	8 cut(s)	142, 316, 414, 1111, 1510, 1629, 1647, 1669
BmuI	ACTGGG	1 cut(s)	2238
BpiI	GAAGAC	1 cut(s)	1518
BpmI	CTGGAG	3 cut(s)	110, 449, 1110
Bpu10I	CCTNAGC	1 cut(s)	963
Bpu1102I	GCTNAGC	1 cut(s)	2277
Bpu14I	TTCGAA	2 cut(s)	881, 1765
Bsa29I	ATCGAT	1 cut(s)	665
BsaAI	YACGTR	1 cut(s)	35
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	1 cut(s)	943
BsaJI	CCNNGG	7 cut(s)	110, 339, 543, 1063, 1064, 1624, 2022
BsaWI	WCCGGW	2 cut(s)	1235, 1332
BsaXI	ACNNNNNCTCC	2 cut(s)	149, 179
Bsc4I	CCNNNNNNNGG	2 cut(s)	995, 1696
Bse1I	ACTGG	1 cut(s)	2233
Bse3DI	GCAATG	2 cut(s)	1584, 1857
BseAI	TCCGGA	2 cut(s)	1235, 1332
BseBI	CCWGG	4 cut(s)	1065, 1748, 1758, 2024
BseCI	ATCGAT	1 cut(s)	665
BseDI	CCNNGG	7 cut(s)	110, 339, 543, 1063, 1064, 1624, 2022
BseLI	CCNNNNNNNGG	2 cut(s)	995, 1696
BseMI	GCAATG	2 cut(s)	1584, 1857
BseMII	CTCAG	5 cut(s)	452, 1035, 1401, 1667, 2319
BseNI	ACTGG	1 cut(s)	2233
BsePI	GCGCGC	1 cut(s)	1988
BseRI	GAGGAG	2 cut(s)	112, 1107
BseSI	GKGCMC	1 cut(s)	901
BseX3I	CGGCCG	1 cut(s)	540
BseXI	GCAGC	8 cut(s)	709, 712, 742, 820, 1601, 1748, 2173, 2310
BsgI	GTGCAG	1 cut(s)	228
Bsh1236I	CGCG	1 cut(s)	1990
Bsh1285I	CGRYCG	1 cut(s)	543
BshFI	GGCC	6 cut(s)	180, 542, 698, 899, 1170, 1746
BshVI	ATCGAT	1 cut(s)	665
BsiEI	CGRYCG	1 cut(s)	543
BsiHKAI	GWGCWC	1 cut(s)	1672
BsiSI	CCGG	2 cut(s)	1236, 1333
BslFI	GGGAC	1 cut(s)	113
BslI	CCNNNNNNNGG	2 cut(s)	995, 1696
BsmAI	GTCTC	8 cut(s)	634, 943, 1018, 1279, 1422, 1874, 1966, 2427
BsmBI	CGTCTC	1 cut(s)	1966
BsmFI	GGGAC	1 cut(s)	113
BsmI	GAATGC	3 cut(s)	1117, 1505, 2472
BsnI	GGCC	6 cut(s)	180, 542, 698, 899, 1170, 1746
Bso31I	GGTCTC	1 cut(s)	943
Bsp119I	TTCGAA	2 cut(s)	881, 1765
Bsp120I	GGGCCC	1 cut(s)	897
Bsp1286I	GDGCHC	2 cut(s)	901, 1672
Bsp13I	TCCGGA	2 cut(s)	1235, 1332
Bsp143I	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
Bsp1720I	GCTNAGC	1 cut(s)	2277
Bsp19I	CCATGG	1 cut(s)	110
BspACI	CCGC	4 cut(s)	23, 539, 758, 2011
BspANI	GGCC	6 cut(s)	180, 542, 698, 899, 1170, 1746
BspCNI	CTCAG	5 cut(s)	451, 1036, 1402, 1666, 2318
BspDI	ATCGAT	1 cut(s)	665
BspEI	TCCGGA	2 cut(s)	1235, 1332
BspFNI	CGCG	1 cut(s)	1990
BspLI	GGNNCC	6 cut(s)	116, 435, 899, 987, 1060, 2352
BspPI	GGATC	2 cut(s)	775, 2475
BspQI	GCTCTTC	1 cut(s)	591
BspT104I	TTCGAA	2 cut(s)	881, 1765
BspTI	CTTAAG	1 cut(s)	950
BspTNI	GGTCTC	1 cut(s)	943
BsrDI	GCAATG	2 cut(s)	1584, 1857
BsrI	ACTGG	1 cut(s)	2233
BssECI	CCNNGG	7 cut(s)	110, 339, 543, 1063, 1064, 1624, 2022
BssHII	GCGCGC	1 cut(s)	1988
BssMI	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	3 cut(s)	110, 339, 1624
Bst2UI	CCWGG	4 cut(s)	1065, 1748, 1758, 2024
Bst4CI	ACNGT	5 cut(s)	455, 574, 1691, 1771, 2087
Bst6I	CTCTTC	7 cut(s)	201, 357, 477, 591, 1277, 1479, 2094
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	1 cut(s)	950
BstAPI	GCANNNNNTGC	2 cut(s)	1322, 1868
BstBAI	YACGTR	1 cut(s)	35
BstBI	TTCGAA	2 cut(s)	881, 1765
BstC8I	GCNNGC	6 cut(s)	1416, 1668, 1990, 2137, 2159, 2445
BstDSI	CCRYGG	1 cut(s)	110
BstFNI	CGCG	1 cut(s)	1990
BstHHI	GCGC	3 cut(s)	1620, 1990, 1992
BstKTI	GATC	7 cut(s)	665, 770, 946, 1037, 1153, 1450, 2483
BstMAI	GTCTC	8 cut(s)	634, 943, 1018, 1279, 1422, 1874, 1966, 2427
BstMBI	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
BstMCI	CGRYCG	1 cut(s)	543
BstMWI	GCNNNNNNNGC	6 cut(s)	802, 1313, 1322, 1626, 1868, 2298
BstNI	CCWGG	4 cut(s)	1065, 1748, 1758, 2024
BstNSI	RCATGY	2 cut(s)	1006, 1204
BstSCI	CCNGG	4 cut(s)	1063, 1746, 1756, 2022
BstSFI	CTRYAG	2 cut(s)	990, 1395
BstSLI	GKGCMC	1 cut(s)	901
BstUI	CGCG	1 cut(s)	1990
BstV1I	GCAGC	8 cut(s)	709, 712, 742, 820, 1601, 1748, 2173, 2310
BstV2I	GAAGAC	1 cut(s)	1518
BstX2I	RGATCY	2 cut(s)	767, 2480
BstXI	CCANNNNNNTGG	1 cut(s)	176
BstYI	RGATCY	2 cut(s)	767, 2480
BstZI	CGGCCG	1 cut(s)	540
Bsu15I	ATCGAT	1 cut(s)	665
BsuI	GTATCC	1 cut(s)	961
BsuRI	GGCC	6 cut(s)	180, 542, 698, 899, 1170, 1746
BsuTUI	ATCGAT	1 cut(s)	665
BtgI	CCRYGG	1 cut(s)	110
BtgZI	GCGATG	1 cut(s)	1593
BtrI	CACGTC	1 cut(s)	2189
BtsI	GCAGTG	2 cut(s)	478, 1839
BtsIMutI	CAGTG	4 cut(s)	460, 478, 1839, 2092
Cac8I	GCNNGC	6 cut(s)	1416, 1668, 1990, 2137, 2159, 2445
CaiI	CAGNNNCTG	2 cut(s)	770, 2170
CfoI	GCGC	3 cut(s)	1620, 1990, 1992
Cfr13I	GGNCC	2 cut(s)	897, 898
ClaI	ATCGAT	1 cut(s)	665
CseI	GACGC	1 cut(s)	19
Csp6I	GTAC	2 cut(s)	1229, 1578
CviQI	GTAC	2 cut(s)	1229, 1578
DpnI	GATC	7 cut(s)	664, 769, 945, 1036, 1152, 1449, 2482
DpnII	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
DraI	TTTAAA	1 cut(s)	2151
EaeI	YGGCCR	3 cut(s)	540, 696, 1168
EagI	CGGCCG	1 cut(s)	540
Eam1104I	CTCTTC	7 cut(s)	201, 357, 477, 591, 1277, 1479, 2094
EarI	CTCTTC	7 cut(s)	201, 357, 477, 591, 1277, 1479, 2094
Ecl136II	GAGCTC	1 cut(s)	1670
EclXI	CGGCCG	1 cut(s)	540
Eco130I	CCWWGG	3 cut(s)	110, 339, 1624
Eco147I	AGGCCT	1 cut(s)	1746
Eco24I	GRGCYC	2 cut(s)	901, 1672
Eco31I	GGTCTC	1 cut(s)	943
Eco52I	CGGCCG	1 cut(s)	540
Eco53kI	GAGCTC	1 cut(s)	1670
Eco57I	CTGAAG	4 cut(s)	381, 917, 1364, 2192
Eco72I	CACGTG	1 cut(s)	35
EcoICRI	GAGCTC	1 cut(s)	1670
EcoO109I	RGGNCCY	1 cut(s)	898
EcoRI	GAATTC	1 cut(s)	1390
EcoRII	CCWGG	4 cut(s)	1063, 1746, 1756, 2022
EcoT14I	CCWWGG	3 cut(s)	110, 339, 1624
EcoT22I	ATGCAT	3 cut(s)	628, 1662, 2472
EcoT38I	GRGCYC	2 cut(s)	901, 1672
ErhI	CCWWGG	3 cut(s)	110, 339, 1624
Esp3I	CGTCTC	1 cut(s)	1966
FaqI	GGGAC	1 cut(s)	113
FbaI	TGATCA	1 cut(s)	943
Fnu4HI	GCNGC	9 cut(s)	540, 723, 726, 756, 834, 1590, 1737, 2162, 2299
FriOI	GRGCYC	2 cut(s)	901, 1672
Fsp4HI	GCNGC	9 cut(s)	540, 723, 726, 756, 834, 1590, 1737, 2162, 2299
FspBI	CTAG	4 cut(s)	248, 1106, 1833, 2288
FspI	TGCGCA	1 cut(s)	1619
GlaI	GCGC	3 cut(s)	1619, 1989, 1991
GluI	GCNGC	9 cut(s)	540, 723, 726, 756, 834, 1590, 1737, 2162, 2299
GsuI	CTGGAG	3 cut(s)	110, 449, 1110
HaeIII	GGCC	6 cut(s)	180, 542, 698, 899, 1170, 1746
HapII	CCGG	2 cut(s)	1236, 1333
HgaI	GACGC	1 cut(s)	19
HhaI	GCGC	3 cut(s)	1620, 1990, 1992
Hin1I	GRCGYC	1 cut(s)	11
Hin6I	GCGC	3 cut(s)	1618, 1988, 1990
HinP1I	GCGC	3 cut(s)	1618, 1988, 1990
HincII	GTYRAC	1 cut(s)	1008
HindII	GTYRAC	1 cut(s)	1008
HindIII	AAGCTT	2 cut(s)	708, 2155
HinfI	GANTC	7 cut(s)	101, 566, 1250, 1487, 2005, 2018, 2474
HpaII	CCGG	2 cut(s)	1236, 1333
HphI	GGTGA	1 cut(s)	1399
Hpy166II	GTNNAC	5 cut(s)	1008, 1706, 1715, 1813, 2083
Hpy188I	TCNGA	7 cut(s)	441, 943, 1381, 1411, 1452, 1656, 2340
Hpy8I	GTNNAC	5 cut(s)	1008, 1706, 1715, 1813, 2083
Hpy99I	CGWCG	2 cut(s)	13, 46
HpyAV	CCTTC	8 cut(s)	179, 394, 529, 577, 733, 1218, 1672, 2008
HpyCH4III	ACNGT	5 cut(s)	455, 574, 1691, 1771, 2087
HpyCH4IV	ACGT	5 cut(s)	34, 779, 1376, 1580, 2188
HpyF10VI	GCNNNNNNNGC	6 cut(s)	802, 1313, 1322, 1626, 1868, 2298
HpySE526I	ACGT	5 cut(s)	34, 779, 1376, 1580, 2188
Hsp92I	GRCGYC	1 cut(s)	11
HspAI	GCGC	3 cut(s)	1618, 1988, 1990
Kpn2I	TCCGGA	2 cut(s)	1235, 1332
Ksp22I	TGATCA	1 cut(s)	943
Kzo9I	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
LguI	GCTCTTC	1 cut(s)	591
LmnI	GCTCC	3 cut(s)	38, 91, 2144
Lsp1109I	GCAGC	8 cut(s)	709, 712, 742, 820, 1601, 1748, 2173, 2310
LweI	GCATC	8 cut(s)	142, 316, 414, 1111, 1510, 1629, 1647, 1669
MaeI	CTAG	4 cut(s)	248, 1106, 1833, 2288
MaeII	ACGT	5 cut(s)	34, 779, 1376, 1580, 2188
MaeIII	GTNAC	4 cut(s)	30, 874, 1255, 2219
MalI	GATC	7 cut(s)	664, 769, 945, 1036, 1152, 1449, 2482
MboI	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
MfeI	CAATTG	1 cut(s)	1683
MflI	RGATCY	2 cut(s)	767, 2480
MhlI	GDGCHC	2 cut(s)	901, 1672
MlyI	GAGTC	1 cut(s)	1496
MmeI	TCCRAC	3 cut(s)	795, 976, 1036
Mph1103I	ATGCAT	3 cut(s)	628, 1662, 2472
MroI	TCCGGA	2 cut(s)	1235, 1332
MseI	TTAA	5 cut(s)	951, 1541, 2112, 2150, 2393
MslI	CAYNNNNRTG	3 cut(s)	174, 1176, 1818
MspCI	CTTAAG	1 cut(s)	950
MspI	CCGG	2 cut(s)	1236, 1333
MspR9I	CCNGG	4 cut(s)	1065, 1748, 1758, 2024
MunI	CAATTG	1 cut(s)	1683
Mva1269I	GAATGC	3 cut(s)	1117, 1505, 2472
MvaI	CCWGG	4 cut(s)	1065, 1748, 1758, 2024
MvnI	CGCG	1 cut(s)	1990
MwoI	GCNNNNNNNGC	6 cut(s)	802, 1313, 1322, 1626, 1868, 2298
NcoI	CCATGG	1 cut(s)	110
NdeII	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
NlaIV	GGNNCC	6 cut(s)	116, 435, 899, 987, 1060, 2352
NmeAIII	GCCGAG	1 cut(s)	568
NmuCI	GTSAC	2 cut(s)	30, 874
NsbI	TGCGCA	1 cut(s)	1619
NsiI	ATGCAT	3 cut(s)	628, 1662, 2472
NspI	RCATGY	2 cut(s)	1006, 1204
NspV	TTCGAA	2 cut(s)	881, 1765
OliI	CACNNNNGTG	1 cut(s)	1818
PasI	CCCWGGG	1 cut(s)	1064
PauI	GCGCGC	1 cut(s)	1988
PceI	AGGCCT	1 cut(s)	1746
PciSI	GCTCTTC	1 cut(s)	591
PcsI	WCGNNNNNNNCGW	2 cut(s)	11, 50
PctI	GAATGC	3 cut(s)	1117, 1505, 2472
PfeI	GAWTC	6 cut(s)	101, 566, 1250, 2005, 2018, 2474
PflFI	GACNNNGTC	1 cut(s)	2230
PkrI	GCNGC	9 cut(s)	541, 724, 727, 757, 835, 1591, 1738, 2163, 2300
PleI	GAGTC	1 cut(s)	1495
PmaCI	CACGTG	1 cut(s)	35
PmlI	CACGTG	1 cut(s)	35
PpsI	GAGTC	1 cut(s)	1495
Ppu21I	YACGTR	1 cut(s)	35
PsiI	TTATAA	1 cut(s)	2121
Psp124BI	GAGCTC	1 cut(s)	1672
Psp1406I	AACGTT	1 cut(s)	779
Psp6I	CCWGG	4 cut(s)	1063, 1746, 1756, 2022
PspCI	CACGTG	1 cut(s)	35
PspGI	CCWGG	4 cut(s)	1063, 1746, 1756, 2022
PspN4I	GGNNCC	6 cut(s)	116, 435, 899, 987, 1060, 2352
PspOMI	GGGCCC	1 cut(s)	897
PspPI	GGNCC	2 cut(s)	897, 898
PstNI	CAGNNNCTG	2 cut(s)	770, 2170
PsuI	RGATCY	2 cut(s)	767, 2480
PsyI	GACNNNGTC	1 cut(s)	2230
PteI	GCGCGC	1 cut(s)	1988
RsaI	GTAC	2 cut(s)	1230, 1579
RsaNI	GTAC	2 cut(s)	1229, 1578
RseI	CAYNNNNRTG	3 cut(s)	174, 1176, 1818
SacI	GAGCTC	1 cut(s)	1672
SapI	GCTCTTC	1 cut(s)	591
SaqAI	TTAA	5 cut(s)	951, 1541, 2112, 2150, 2393
SatI	GCNGC	9 cut(s)	540, 723, 726, 756, 834, 1590, 1737, 2162, 2299
Sau3AI	GATC	7 cut(s)	662, 767, 943, 1034, 1150, 1447, 2480
Sau96I	GGNCC	2 cut(s)	897, 898
SchI	GAGTC	1 cut(s)	1496
ScrFI	CCNGG	4 cut(s)	1065, 1748, 1758, 2024
SduI	GDGCHC	2 cut(s)	901, 1672
SfaNI	GCATC	8 cut(s)	142, 316, 414, 1111, 1510, 1629, 1647, 1669
SfcI	CTRYAG	2 cut(s)	990, 1395
SfuI	TTCGAA	2 cut(s)	881, 1765
SmiMI	CAYNNNNRTG	3 cut(s)	174, 1176, 1818
SmlI	CTYRAG	1 cut(s)	950
SmoI	CTYRAG	1 cut(s)	950
SseBI	AGGCCT	1 cut(s)	1746
SsiI	CCGC	4 cut(s)	23, 539, 758, 2011
SspMI	CTAG	4 cut(s)	248, 1106, 1833, 2288
SstI	GAGCTC	1 cut(s)	1672
StuI	AGGCCT	1 cut(s)	1746
StyD4I	CCNGG	4 cut(s)	1063, 1746, 1756, 2022
StyI	CCWWGG	3 cut(s)	110, 339, 1624
TaaI	ACNGT	5 cut(s)	455, 574, 1691, 1771, 2087
TaiI	ACGT	5 cut(s)	37, 782, 1379, 1583, 2191
TaqI	TCGA	7 cut(s)	183, 268, 665, 881, 1153, 1482, 1765
TauI	GCSGC	1 cut(s)	542
TfiI	GAWTC	6 cut(s)	101, 566, 1250, 2005, 2018, 2474
Tru1I	TTAA	5 cut(s)	951, 1541, 2112, 2150, 2393
Tru9I	TTAA	5 cut(s)	951, 1541, 2112, 2150, 2393
TscAI	CASTG	4 cut(s)	460, 478, 1846, 2092
TseFI	GTSAC	2 cut(s)	30, 874
TseI	GCWGC	8 cut(s)	722, 725, 755, 833, 1589, 1736, 2161, 2298
Tsp45I	GTSAC	2 cut(s)	30, 874
TspGWI	ACGGA	1 cut(s)	1025
TspRI	CASTG	4 cut(s)	460, 478, 1846, 2092
Tth111I	GACNNNGTC	1 cut(s)	2230
Vha464I	CTTAAG	1 cut(s)	950
XapI	RAATTY	5 cut(s)	66, 237, 1390, 1889, 2146
XceI	RCATGY	2 cut(s)	1006, 1204
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1071
XspI	CTAG	4 cut(s)	248, 1106, 1833, 2288
Zsp2I	ATGCAT	3 cut(s)	628, 1662, 2472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.