Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004055

Physical Location & Seq

Genomic Coordinates Reverse (-)

11899876 .. 11907616

Gene Structure 7741 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022766

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGACGCCGACGCCGGTGCCACCGCCTCCGTCACGTGGAGCGACGAGGAAAGCGAAGAAGAAACAAATTCAACAGCAACACGCAGAGCTGGAGCGTTTGGATTCTCTCCCATGGAATCCCTCGTCTGTCCCTCTCCCTAATGACAATGACGATGCTGACCCTTTCTCTCATCTCAATGGCCTCGAAGGAGGTTTTCTTATGCTTGAAGAGATTGATGAGGCTGATTATGGCTTAGAAATTCCTGCACTAGAAGTAGAAAATAGAAATCAAAACGATAAGTCTAAGCCAACTAAAAAGTCCAAGAAACGAAAGCATGACCAACTTGATGCAAGTGATACCTTGGTTGAGGAGGTTATTGGTGAAGAGAGATTGGAAAAGGATGAGAAACATATGACTAAGAAGGAGAAAACGAAGAAGAAGAAGAAGAAGAAGCTGGAGGAACCTCAGAAAATTGAGGATGCTGATGATAATTGTGGCAGTGAAGTTGAAGAGAAAGACAATGATGGGGATGTAGGTTCAAAGAAGAAAAAGAAGAAGAAGAAGAAGGCGGCCGAGGAAAGCCAGAAAAATGAGGAATCCACAGTTGATGAGAAGGATGAAGTAGAAGAGCAAGTTGCTGAGGATGAATACTATGCATGGAATGAGTTGAGACTTCATCCCCTGATTATGAGATCGATATACAGGCTTGGGTTCAAAGAACCAACGGCCATCCAGAAAGCTTGTATTCCTGCTGCTGCTCATCAAGGGAAGGATGTTGTTGGTGCTGCGGAAACAGGATCTGGCAAAACGTTAGCTTTTGGTCTACCAATTTTGCAACGCCTGTTGGACGAAAGAGAAAAGGCTGCGAAGATGTTTGAAGAAAAGGGAGAGGAAGCAGAAAAAGTCACTTCAAAAGGCTTTTTACGGGCCCTTGTTATTACTCCTACAAGAGAACTTTCACTTCAGGTCTCTGATCATCTTAAGGCGGTTGCTAAGGATACTGATATTAGGGTGGTTCCAATAGTTGGAGGCATGTCAACGGACAAGCAGGAGAGACTCCTTAGATCAAGACCTGAGATTGTTGTTGGAACCCCAGGGAGATTATGGGAACTTATGTCTGGAGGAGAAAAGCATCTAGTTGAGTTGCATTCATTGTCTTTCTTTGTGCTGGATGAGGCTGATCGAATGATAGAAAACGGCCATTTCCATGAACTGCAGTCTATTATTGACATGCTTCCTTTAAGGAATAGTTCTGCTGTACATTCTGGAAATGCACAAGATTCTGTTACCATGACCAACTTCCAGAGGAAGAAGAGACAAACATTTGTTTTATCTGCAACAATAGCACTTTCTGCTGATTTCCGGAAAAAGCTGAAGCGTAGTTCTCTAAAACCAAAGCAATCAACGTCTGATGGGGTGAATTCTATAGAAGCTCTCTCTGAGCGAGCAGGGATGAGAGACAATGTTGCCATCATTGATCTGACAAATACATCCATTTTGGCGAATAAGCTCGAAGAGTCATTTATTGAATGCAGGGAAGAAGACAAAGATGCCTATTTGTATTATATATTAAGTGTCCACGGGCAAGGTCGCACAATCATATTCTGTACGTCCATTGCAGCTTTGCGACATATAACATCACTCTTGCGCATCCTTGGCACAGATGTTTTGACACTTCATGCTCAGATGCATCAGCGAGCTCGTTTGAAGGCAATTGACCGTTTTAGGGGGAGTGAACATGGTAAACTTGTGGCTACTGATGTTGCAGCAAGAGGCCTGGATATTCCTGGTGTTCGAACTGTTATTCACTACCAGCTTCCCCATTCAGCAGAAGTTTATGTTCACAGAAGTGGTAGAACTGCTAGAGCCACTGCTGATGGGTGTAGCATTGCACTAATTGCACCAAATGAGACATCCAAATTTGCTTCTTTGTGCAAATCATTTTCTAAGGTAAGTTTTCAACGATTTCCTTTGGAGAATTCATACTTCCCAGAGGTTATGAGACGATTATCTCTTGCGCGCCAAATAGACAAGATTCTGCGGAAGGATTCCCAGGACAAAGCAAAGAAAAGCTGGTATGAAAGAAATGCTGAATTGGTTGAATTGGTTGTGGACAGTGATGAGGATGAAGAGGATAGAGTTAAGAATTATAAGCAAAAGAAAGCCAGCTCCATGAATTTAAAAAGCTTGCAGCAGGAACTGAAGATGTTGCTTTCACGTCCATTACAACCAAAGTCATTTTCACATCGTTACTTGACTGGGTCTGGTATCACACCTCTGGTGCAACATCAATTTGAAGAATTGGCTAAGCAAAAACTAGGCGATAGCAGCAACTCAGGAAAAAGAAGTAAATTGTTAGTTATTGGTCAGAATTGTGTGGAACCACTCCAAGCACTTCGGAGTTCTGGGCATGAGGTGCACATAGATGTGAAAGAGGCGGCACAAAAACGAAGGAATATGGAGAACCTAAGGAGGAAAAGAAAAGAGGAGAAAAAACGGTTGAGAGATGACCGCAGAAAGAGGAAGAAACAGCTAAAAGGGGGAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

843

Amino Acids

95.28

Weight (kDa)

9.12

Isoelectric Point (pI)

49.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	235 - 442	5.3e-39	DEAD/DEAH box helicase
Helicase_C	PF00271	509 - 616	1.3e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16840 AT3G16840
fragaria_vesca	FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910 FvH4_1g29910
malus_domestica	MD12G1094500.v1.1 MD14G1084300.v1.1
prunus_persica	Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1 Prupe.5G037600_v2.0.a1
pyrus_communis	pycom14g07400
rosa_chinensis	RchiOBHm_Chr3g0492441
rosa_laevigata	RLG00000022766
rosa_multiflora	Rmu_sc0003156.1_g000008 Rmu_sc0007709.1_g000003 Rmu_sc0017916.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392260
rosa_rugosa	Rorug03G0253900
rosa_samantha	Rh3AG304100 Rh3BG340000 Rh3CG337200 Rh3DG339200
rosa_wichuraiana	Rw3G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2130
Acc16I	TGCGCA	1 cut(s)	1628
AccB1I	GGYRCC	1 cut(s)	16
AccB7I	CCANNNNNTGG	1 cut(s)	1004
AccI	GTMKAC	1 cut(s)	802
AccII	CGCG	1 cut(s)	1999
AccIII	TCCGGA	1 cut(s)	1341
AciI	CCGC	7 cut(s)	23, 548, 767, 965, 2020, 2417, 2491
AclI	AACGTT	1 cut(s)	788
AclWI	GGATC	1 cut(s)	784
AcoI	YGGCCR	3 cut(s)	549, 705, 1177
AcsI	RAATTY	6 cut(s)	66, 237, 1399, 1898, 1957, 2155
AcuI	CTGAAG	3 cut(s)	926, 1373, 2201
AcvI	CACGTG	1 cut(s)	35
AcyI	GRCGYC	2 cut(s)	5, 11
AfaI	GTAC	2 cut(s)	1239, 1588
AfiI	CCNNNNNNNGG	3 cut(s)	35, 1004, 1705
AflII	CTTAAG	1 cut(s)	959
AjiI	CACGTC	1 cut(s)	2198
AjnI	CCWGG	4 cut(s)	1072, 1755, 1765, 2031
AjuI	GAANNNNNNNTTGG	2 cut(s)	2203, 2235
AleI	CACNNNNGTG	1 cut(s)	1827
Alw21I	GWGCWC	2 cut(s)	1681, 2400
Alw26I	GTCTC	7 cut(s)	643, 952, 1027, 1288, 1431, 1883, 1975
Alw44I	GTGCAC	1 cut(s)	2396
AlwI	GGATC	1 cut(s)	784
AlwNI	CAGNNNCTG	2 cut(s)	779, 2179
Aor13HI	TCCGGA	1 cut(s)	1341
AoxI	GGCC	6 cut(s)	178, 549, 705, 906, 1177, 1753
ApaI	GGGCCC	1 cut(s)	910
ApaLI	GTGCAC	1 cut(s)	2396
ApeKI	GCWGC	8 cut(s)	731, 734, 764, 842, 1598, 1745, 2170, 2307
ApoI	RAATTY	6 cut(s)	66, 237, 1399, 1898, 1957, 2155
AspLEI	GCGC	3 cut(s)	1629, 1999, 2001
AspS9I	GGNCC	2 cut(s)	906, 907
AsuHPI	GGTGA	2 cut(s)	371, 1408
AsuII	TTCGAA	1 cut(s)	1774
AxyI	CCTNAGG	1 cut(s)	2447
BaeGI	GKGCMC	2 cut(s)	910, 2400
BaeI	ACNNNNGTAYC	2 cut(s)	327, 360
BanI	GGYRCC	1 cut(s)	16
BanII	GRGCYC	2 cut(s)	910, 1681
BbrPI	CACGTG	1 cut(s)	35
BbsI	GAAGAC	1 cut(s)	1527
Bbv12I	GWGCWC	2 cut(s)	1681, 2400
BbvCI	CCTCAGC	1 cut(s)	618
BbvI	GCAGC	8 cut(s)	718, 721, 751, 829, 1610, 1757, 2182, 2319
BccI	CCATC	5 cut(s)	497, 716, 1385, 1457, 1850
BceAI	ACGGC	2 cut(s)	720, 1192
BcgI	CGANNNNNNTGC	1 cut(s)	32
BciT130I	CCWGG	4 cut(s)	1074, 1757, 1767, 2033
BciVI	GTATCC	1 cut(s)	970
BclI	TGATCA	1 cut(s)	952
BcoDI	GTCTC	7 cut(s)	643, 952, 1027, 1288, 1431, 1883, 1975
BfaI	CTAG	4 cut(s)	248, 1115, 1842, 2297
BfmI	CTRYAG	2 cut(s)	1193, 1404
BfrI	CTTAAG	1 cut(s)	959
BfuI	GTATCC	1 cut(s)	970
BlpI	GCTNAGC	1 cut(s)	2286
Bme1390I	CCNGG	4 cut(s)	1074, 1757, 1767, 2033
BmgBI	CACGTC	1 cut(s)	2198
BmgT120I	GGNCC	2 cut(s)	906, 907
BmiI	GGNNCC	6 cut(s)	18, 441, 908, 996, 1069, 2361
BmrFI	CCNGG	4 cut(s)	1074, 1757, 1767, 2033
BmrI	ACTGGG	1 cut(s)	2247
BmsI	GCATC	8 cut(s)	142, 316, 448, 1120, 1519, 1638, 1656, 1678
BmuI	ACTGGG	1 cut(s)	2247
BpiI	GAAGAC	1 cut(s)	1527
BpmI	CTGGAG	3 cut(s)	110, 455, 1119
Bpu10I	CCTNAGC	2 cut(s)	618, 972
Bpu1102I	GCTNAGC	1 cut(s)	2286
Bpu14I	TTCGAA	1 cut(s)	1774
Bsa29I	ATCGAT	1 cut(s)	674
BsaAI	YACGTR	1 cut(s)	35
BsaHI	GRCGYC	2 cut(s)	5, 11
BsaI	GGTCTC	1 cut(s)	952
BsaJI	CCNNGG	8 cut(s)	110, 339, 552, 1072, 1073, 1558, 1633, 2031
BsaWI	WCCGGW	1 cut(s)	1341
Bsc4I	CCNNNNNNNGG	3 cut(s)	35, 1004, 1705
Bse118I	RCCGGY	1 cut(s)	13
Bse1I	ACTGG	1 cut(s)	2242
Bse21I	CCTNAGG	1 cut(s)	2447
Bse3DI	GCAATG	2 cut(s)	1593, 1866
BseAI	TCCGGA	1 cut(s)	1341
BseBI	CCWGG	4 cut(s)	1074, 1757, 1767, 2033
BseCI	ATCGAT	1 cut(s)	674
BseDI	CCNNGG	8 cut(s)	110, 339, 552, 1072, 1073, 1558, 1633, 2031
BseLI	CCNNNNNNNGG	3 cut(s)	35, 1004, 1705
BseMI	GCAATG	2 cut(s)	1593, 1866
BseMII	CTCAG	6 cut(s)	458, 609, 1044, 1410, 1676, 2328
BseNI	ACTGG	1 cut(s)	2242
BsePI	GCGCGC	1 cut(s)	1997
BseRI	GAGGAG	3 cut(s)	362, 1116, 2480
BseSI	GKGCMC	2 cut(s)	910, 2400
BseX3I	CGGCCG	1 cut(s)	549
BseXI	GCAGC	8 cut(s)	718, 721, 751, 829, 1610, 1757, 2182, 2319
BsgI	GTGCAG	1 cut(s)	228
Bsh1236I	CGCG	1 cut(s)	1999
Bsh1285I	CGRYCG	1 cut(s)	552
BshFI	GGCC	6 cut(s)	180, 551, 707, 908, 1179, 1755
BshNI	GGYRCC	1 cut(s)	16
BshVI	ATCGAT	1 cut(s)	674
BsiEI	CGRYCG	1 cut(s)	552
BsiHKAI	GWGCWC	2 cut(s)	1681, 2400
BsiSI	CCGG	2 cut(s)	14, 1342
BslFI	GGGAC	1 cut(s)	113
BslI	CCNNNNNNNGG	3 cut(s)	35, 1004, 1705
BsmAI	GTCTC	7 cut(s)	643, 952, 1027, 1288, 1431, 1883, 1975
BsmBI	CGTCTC	1 cut(s)	1975
BsmFI	GGGAC	1 cut(s)	113
BsmI	GAATGC	2 cut(s)	1126, 1514
BsnI	GGCC	6 cut(s)	180, 551, 707, 908, 1179, 1755
Bso31I	GGTCTC	1 cut(s)	952
Bsp119I	TTCGAA	1 cut(s)	1774
Bsp120I	GGGCCC	1 cut(s)	906
Bsp1286I	GDGCHC	3 cut(s)	910, 1681, 2400
Bsp13I	TCCGGA	1 cut(s)	1341
Bsp1407I	TGTACA	1 cut(s)	1237
Bsp143I	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
Bsp1720I	GCTNAGC	1 cut(s)	2286
Bsp19I	CCATGG	1 cut(s)	110
BspACI	CCGC	7 cut(s)	23, 548, 767, 965, 2020, 2417, 2491
BspANI	GGCC	6 cut(s)	180, 551, 707, 908, 1179, 1755
BspCNI	CTCAG	6 cut(s)	457, 610, 1045, 1411, 1675, 2327
BspDI	ATCGAT	1 cut(s)	674
BspEI	TCCGGA	1 cut(s)	1341
BspFNI	CGCG	1 cut(s)	1999
BspLI	GGNNCC	6 cut(s)	18, 441, 908, 996, 1069, 2361
BspMAI	CTGCAG	1 cut(s)	1197
BspPI	GGATC	1 cut(s)	784
BspQI	GCTCTTC	1 cut(s)	600
BspT104I	TTCGAA	1 cut(s)	1774
BspT107I	GGYRCC	1 cut(s)	16
BspTI	CTTAAG	1 cut(s)	959
BspTNI	GGTCTC	1 cut(s)	952
BsrDI	GCAATG	2 cut(s)	1593, 1866
BsrFI	RCCGGY	1 cut(s)	13
BsrGI	TGTACA	1 cut(s)	1237
BsrI	ACTGG	1 cut(s)	2242
BssAI	RCCGGY	1 cut(s)	13
BssECI	CCNNGG	8 cut(s)	110, 339, 552, 1072, 1073, 1558, 1633, 2031
BssHII	GCGCGC	1 cut(s)	1997
BssMI	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
BssNI	GRCGYC	2 cut(s)	5, 11
BssT1I	CCWWGG	3 cut(s)	110, 339, 1633
Bst2UI	CCWGG	4 cut(s)	1074, 1757, 1767, 2033
Bst4CI	ACNGT	5 cut(s)	583, 1700, 1780, 2096, 2478
Bst6I	CTCTTC	7 cut(s)	201, 357, 483, 600, 1286, 1488, 2103
BstACI	GRCGYC	2 cut(s)	5, 11
BstAFI	CTTAAG	1 cut(s)	959
BstAPI	GCANNNNNTGC	3 cut(s)	1331, 1877, 2395
BstAUI	TGTACA	1 cut(s)	1237
BstBAI	YACGTR	1 cut(s)	35
BstBI	TTCGAA	1 cut(s)	1774
BstC8I	GCNNGC	5 cut(s)	1425, 1677, 1999, 2146, 2168
BstDSI	CCRYGG	2 cut(s)	110, 1558
BstFNI	CGCG	1 cut(s)	1999
BstHHI	GCGC	3 cut(s)	1629, 1999, 2001
BstKTI	GATC	6 cut(s)	674, 779, 955, 1046, 1162, 1459
BstMAI	GTCTC	7 cut(s)	643, 952, 1027, 1288, 1431, 1883, 1975
BstMBI	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
BstMCI	CGRYCG	1 cut(s)	552
BstMWI	GCNNNNNNNGC	6 cut(s)	1322, 1331, 1635, 1877, 2307, 2395
BstNI	CCWGG	4 cut(s)	1074, 1757, 1767, 2033
BstNSI	RCATGY	2 cut(s)	1015, 1213
BstSCI	CCNGG	4 cut(s)	1072, 1755, 1765, 2031
BstSFI	CTRYAG	2 cut(s)	1193, 1404
BstSLI	GKGCMC	2 cut(s)	910, 2400
BstUI	CGCG	1 cut(s)	1999
BstV1I	GCAGC	8 cut(s)	718, 721, 751, 829, 1610, 1757, 2182, 2319
BstV2I	GAAGAC	1 cut(s)	1527
BstX2I	RGATCY	1 cut(s)	776
BstYI	RGATCY	1 cut(s)	776
BstZI	CGGCCG	1 cut(s)	549
Bsu15I	ATCGAT	1 cut(s)	674
Bsu36I	CCTNAGG	1 cut(s)	2447
BsuI	GTATCC	1 cut(s)	970
BsuRI	GGCC	6 cut(s)	180, 551, 707, 908, 1179, 1755
BsuTUI	ATCGAT	1 cut(s)	674
BtgI	CCRYGG	2 cut(s)	110, 1558
BtrI	CACGTC	1 cut(s)	2198
BtsI	GCAGTG	2 cut(s)	484, 1848
BtsIMutI	CAGTG	3 cut(s)	484, 1848, 2101
Cac8I	GCNNGC	5 cut(s)	1425, 1677, 1999, 2146, 2168
CaiI	CAGNNNCTG	2 cut(s)	779, 2179
CfoI	GCGC	3 cut(s)	1629, 1999, 2001
Cfr10I	RCCGGY	1 cut(s)	13
Cfr13I	GGNCC	2 cut(s)	906, 907
ClaI	ATCGAT	1 cut(s)	674
CseI	GACGC	2 cut(s)	13, 19
Csp6I	GTAC	2 cut(s)	1238, 1587
CviQI	GTAC	2 cut(s)	1238, 1587
DpnI	GATC	6 cut(s)	673, 778, 954, 1045, 1161, 1458
DpnII	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
DraI	TTTAAA	1 cut(s)	2160
EaeI	YGGCCR	3 cut(s)	549, 705, 1177
EagI	CGGCCG	1 cut(s)	549
Eam1104I	CTCTTC	7 cut(s)	201, 357, 483, 600, 1286, 1488, 2103
EarI	CTCTTC	7 cut(s)	201, 357, 483, 600, 1286, 1488, 2103
Ecl136II	GAGCTC	1 cut(s)	1679
EclXI	CGGCCG	1 cut(s)	549
Eco130I	CCWWGG	3 cut(s)	110, 339, 1633
Eco147I	AGGCCT	1 cut(s)	1755
Eco24I	GRGCYC	2 cut(s)	910, 1681
Eco31I	GGTCTC	1 cut(s)	952
Eco52I	CGGCCG	1 cut(s)	549
Eco53kI	GAGCTC	1 cut(s)	1679
Eco57I	CTGAAG	3 cut(s)	926, 1373, 2201
Eco72I	CACGTG	1 cut(s)	35
Eco81I	CCTNAGG	1 cut(s)	2447
EcoICRI	GAGCTC	1 cut(s)	1679
EcoO109I	RGGNCCY	1 cut(s)	907
EcoRI	GAATTC	2 cut(s)	1399, 1957
EcoRII	CCWGG	4 cut(s)	1072, 1755, 1765, 2031
EcoT14I	CCWWGG	3 cut(s)	110, 339, 1633
EcoT22I	ATGCAT	2 cut(s)	637, 1671
EcoT38I	GRGCYC	2 cut(s)	910, 1681
ErhI	CCWWGG	3 cut(s)	110, 339, 1633
Esp3I	CGTCTC	1 cut(s)	1975
FaqI	GGGAC	1 cut(s)	113
FauNDI	CATATG	1 cut(s)	390
FbaI	TGATCA	1 cut(s)	952
FblI	GTMKAC	1 cut(s)	802
FriOI	GRGCYC	2 cut(s)	910, 1681
FspBI	CTAG	4 cut(s)	248, 1115, 1842, 2297
FspI	TGCGCA	1 cut(s)	1628
GlaI	GCGC	3 cut(s)	1628, 1998, 2000
GsuI	CTGGAG	3 cut(s)	110, 455, 1119
HaeIII	GGCC	6 cut(s)	180, 551, 707, 908, 1179, 1755
HapII	CCGG	2 cut(s)	14, 1342
HgaI	GACGC	2 cut(s)	13, 19
HhaI	GCGC	3 cut(s)	1629, 1999, 2001
Hin1I	GRCGYC	2 cut(s)	5, 11
Hin6I	GCGC	3 cut(s)	1627, 1997, 1999
HinP1I	GCGC	3 cut(s)	1627, 1997, 1999
HincII	GTYRAC	1 cut(s)	1017
HindII	GTYRAC	1 cut(s)	1017
HindIII	AAGCTT	2 cut(s)	717, 2164
HinfI	GANTC	8 cut(s)	101, 115, 575, 1035, 1259, 1496, 2014, 2027
HpaII	CCGG	2 cut(s)	14, 1342
HphI	GGTGA	2 cut(s)	371, 1408
Hpy166II	GTNNAC	8 cut(s)	803, 1017, 1558, 1715, 1724, 1822, 2092, 2398
Hpy188I	TCNGA	8 cut(s)	447, 952, 1390, 1420, 1461, 1665, 2349, 2379
Hpy188III	TCNNGA	7 cut(s)	712, 1047, 1098, 1245, 1282, 1342, 2316
Hpy8I	GTNNAC	8 cut(s)	803, 1017, 1558, 1715, 1724, 1822, 2092, 2398
Hpy99I	CGWCG	2 cut(s)	13, 46
HpyAV	CCTTC	8 cut(s)	179, 394, 538, 586, 742, 1681, 2017, 2424
HpyCH4III	ACNGT	5 cut(s)	583, 1700, 1780, 2096, 2478
HpyCH4IV	ACGT	5 cut(s)	34, 788, 1385, 1589, 2197
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1322, 1331, 1635, 1877, 2307, 2395
HpySE526I	ACGT	5 cut(s)	34, 788, 1385, 1589, 2197
Hsp92I	GRCGYC	2 cut(s)	5, 11
HspAI	GCGC	3 cut(s)	1627, 1997, 1999
Kpn2I	TCCGGA	1 cut(s)	1341
Ksp22I	TGATCA	1 cut(s)	952
Kzo9I	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
LguI	GCTCTTC	1 cut(s)	600
LmnI	GCTCC	3 cut(s)	38, 91, 2153
Lsp1109I	GCAGC	8 cut(s)	718, 721, 751, 829, 1610, 1757, 2182, 2319
LweI	GCATC	8 cut(s)	142, 316, 448, 1120, 1519, 1638, 1656, 1678
MaeI	CTAG	4 cut(s)	248, 1115, 1842, 2297
MaeII	ACGT	5 cut(s)	34, 788, 1385, 1589, 2197
MaeIII	GTNAC	4 cut(s)	30, 883, 1264, 2228
MalI	GATC	6 cut(s)	673, 778, 954, 1045, 1161, 1458
MboI	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
MfeI	CAATTG	1 cut(s)	1692
MflI	RGATCY	1 cut(s)	776
MhlI	GDGCHC	3 cut(s)	910, 1681, 2400
MlyI	GAGTC	2 cut(s)	1029, 1505
MmeI	TCCRAC	3 cut(s)	804, 985, 1045
Mph1103I	ATGCAT	2 cut(s)	637, 1671
MroI	TCCGGA	1 cut(s)	1341
MseI	TTAA	5 cut(s)	960, 1220, 1550, 2121, 2159
MslI	CAYNNNNRTG	5 cut(s)	174, 1185, 1827, 2403, 2405
MspCI	CTTAAG	1 cut(s)	959
MspI	CCGG	2 cut(s)	14, 1342
MspR9I	CCNGG	4 cut(s)	1074, 1757, 1767, 2033
MunI	CAATTG	1 cut(s)	1692
Mva1269I	GAATGC	2 cut(s)	1126, 1514
MvaI	CCWGG	4 cut(s)	1074, 1757, 1767, 2033
MvnI	CGCG	1 cut(s)	1999
MwoI	GCNNNNNNNGC	6 cut(s)	1322, 1331, 1635, 1877, 2307, 2395
NcoI	CCATGG	1 cut(s)	110
NdeI	CATATG	1 cut(s)	390
NdeII	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
NlaIV	GGNNCC	6 cut(s)	18, 441, 908, 996, 1069, 2361
NmeAIII	GCCGAG	1 cut(s)	577
NmuCI	GTSAC	2 cut(s)	30, 883
NsbI	TGCGCA	1 cut(s)	1628
NsiI	ATGCAT	2 cut(s)	637, 1671
NspI	RCATGY	2 cut(s)	1015, 1213
NspV	TTCGAA	1 cut(s)	1774
OliI	CACNNNNGTG	1 cut(s)	1827
PasI	CCCWGGG	1 cut(s)	1073
PauI	GCGCGC	1 cut(s)	1997
PceI	AGGCCT	1 cut(s)	1755
PciSI	GCTCTTC	1 cut(s)	600
PcsI	WCGNNNNNNNCGW	1 cut(s)	50
PctI	GAATGC	2 cut(s)	1126, 1514
PfeI	GAWTC	6 cut(s)	101, 115, 575, 1259, 2014, 2027
PflFI	GACNNNGTC	1 cut(s)	2239
PflMI	CCANNNNNTGG	1 cut(s)	1004
PleI	GAGTC	2 cut(s)	1029, 1504
PmaCI	CACGTG	1 cut(s)	35
PmlI	CACGTG	1 cut(s)	35
PpsI	GAGTC	2 cut(s)	1029, 1504
Ppu21I	YACGTR	1 cut(s)	35
PsiI	TTATAA	1 cut(s)	2130
Psp124BI	GAGCTC	1 cut(s)	1681
Psp1406I	AACGTT	1 cut(s)	788
Psp6I	CCWGG	4 cut(s)	1072, 1755, 1765, 2031
PspCI	CACGTG	1 cut(s)	35
PspGI	CCWGG	4 cut(s)	1072, 1755, 1765, 2031
PspN4I	GGNNCC	6 cut(s)	18, 441, 908, 996, 1069, 2361
PspOMI	GGGCCC	1 cut(s)	906
PspPI	GGNCC	2 cut(s)	906, 907
PstI	CTGCAG	1 cut(s)	1197
PstNI	CAGNNNCTG	2 cut(s)	779, 2179
PsuI	RGATCY	1 cut(s)	776
PsyI	GACNNNGTC	1 cut(s)	2239
PteI	GCGCGC	1 cut(s)	1997
RsaI	GTAC	2 cut(s)	1239, 1588
RsaNI	GTAC	2 cut(s)	1238, 1587
RseI	CAYNNNNRTG	5 cut(s)	174, 1185, 1827, 2403, 2405
SacI	GAGCTC	1 cut(s)	1681
SapI	GCTCTTC	1 cut(s)	600
SaqAI	TTAA	5 cut(s)	960, 1220, 1550, 2121, 2159
Sau3AI	GATC	6 cut(s)	671, 776, 952, 1043, 1159, 1456
Sau96I	GGNCC	2 cut(s)	906, 907
SchI	GAGTC	2 cut(s)	1029, 1505
ScrFI	CCNGG	4 cut(s)	1074, 1757, 1767, 2033
SduI	GDGCHC	3 cut(s)	910, 1681, 2400
SfaNI	GCATC	8 cut(s)	142, 316, 448, 1120, 1519, 1638, 1656, 1678
SfcI	CTRYAG	2 cut(s)	1193, 1404
SfuI	TTCGAA	1 cut(s)	1774
SgrAI	CRCCGGYG	1 cut(s)	13
SmiMI	CAYNNNNRTG	5 cut(s)	174, 1185, 1827, 2403, 2405
SmlI	CTYRAG	1 cut(s)	959
SmoI	CTYRAG	1 cut(s)	959
SseBI	AGGCCT	1 cut(s)	1755
SsiI	CCGC	7 cut(s)	23, 548, 767, 965, 2020, 2417, 2491
SspMI	CTAG	4 cut(s)	248, 1115, 1842, 2297
SstI	GAGCTC	1 cut(s)	1681
StuI	AGGCCT	1 cut(s)	1755
StyD4I	CCNGG	4 cut(s)	1072, 1755, 1765, 2031
StyI	CCWWGG	3 cut(s)	110, 339, 1633
TaaI	ACNGT	5 cut(s)	583, 1700, 1780, 2096, 2478
TaiI	ACGT	5 cut(s)	37, 791, 1388, 1592, 2200
TaqI	TCGA	5 cut(s)	183, 674, 1162, 1491, 1774
TatI	WGTACW	1 cut(s)	1237
TauI	GCSGC	2 cut(s)	551, 2420
TfiI	GAWTC	6 cut(s)	101, 115, 575, 1259, 2014, 2027
Tru1I	TTAA	5 cut(s)	960, 1220, 1550, 2121, 2159
Tru9I	TTAA	5 cut(s)	960, 1220, 1550, 2121, 2159
TscAI	CASTG	3 cut(s)	484, 1855, 2101
TseFI	GTSAC	2 cut(s)	30, 883
TseI	GCWGC	8 cut(s)	731, 734, 764, 842, 1598, 1745, 2170, 2307
Tsp45I	GTSAC	2 cut(s)	30, 883
TspGWI	ACGGA	2 cut(s)	18, 1034
TspRI	CASTG	3 cut(s)	484, 1855, 2101
Tth111I	GACNNNGTC	1 cut(s)	2239
Van91I	CCANNNNNTGG	1 cut(s)	1004
Vha464I	CTTAAG	1 cut(s)	959
VneI	GTGCAC	1 cut(s)	2396
XapI	RAATTY	6 cut(s)	66, 237, 1399, 1898, 1957, 2155
XceI	RCATGY	2 cut(s)	1015, 1213
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1080
XmiI	GTMKAC	1 cut(s)	802
XspI	CTAG	4 cut(s)	248, 1115, 1842, 2297
Zsp2I	ATGCAT	2 cut(s)	637, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.