Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013898

Physical Location & Seq

Genomic Coordinates Forward (+)

22976959 .. 22980558

Gene Structure 3600 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1149600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGGAAAACAGACTAGTTTCTCTTCTCATTCTCACAGTTTCAATGCTATGCTGTACAGGACGAAACTTAGCCACAGTATATCGCTGCCAGGACTGCGGCCACACTCCGGTGCCCTACCCGCTAAGCACATCACCAGACTGCGGAAACCAGCACTACAAAGTCCGATGCCATGCAGGGTCACTGTGGTTTGACGCCCTCAACAGCTCATCATATTTAATCACATCAATTAACCCACTAACCCGGCGTCTAATTATCCGGCCGCCGGGGTTAGCCAACAACGTTACGTGCATGTCGGCCGACTTTAAATCCCAAGGCCTAATACTTGACGACAACCTCCCTTTCAACATCACCAGCAGCAACACAGTGATAGGCATGAACTGTTCCTATGAAATGCTTACTCTCTCGAAAAACTGCTCATCAAATAGCTTATGCCACGACTACATTAAGCACAACGCAATGGCTGCCTCCGCTTGCGGGTGCTTCCAGATTTGTTGTTTATTCAAGACTGGTGGGTCTACCAATGCCTACAAAATTAGGGTTATGAAGGAAAGGTGTTCGGCTTATGAGAGTTTTGTCAATTTGGACTCTTTTTCTGGGCCTTTGAACAGTTGGCCTGAGTCTGGGGTGGAGATGATGTGGGAATCGCCGCGGGAGCCGATGTGCAAGGTTTCAGTTGATTGTCGGGGTTTGTCTAACTCGGAGTGTTTGGTAGATCGGAGTGGACAAAGAAGGTGCTTGTGCAAGACTGGTTTTGAATGGGACCCCATCAATGCAATATGTCGAAGCTTGATCATTGCTGCCGTTGCAATGCTACTTGGAGCCTCACTTGCATTCATAGTCTACAAGCGACGCGAAAAGAAAATTGCCAGAGCAGCACATTTTAGCTTGACCAAGTCCCGGGAAGACATGTTAAATGCAAACAATAATACCGGAAAATCAGCCAAGCTCTTCACAGGCAAAGAAATCACGAGAGCAACCAAAAATTTTTCCAAAGATAAAGTTCTTGGTGCCGGTGGCTTTGGTGAGGTCTTCAAAGGTGTTCTAGATGATGGAACTATCACTGCAGTAAAGAGAGCCCAGCCCGGGAACACAAAAGGCATGGATCAAATTCTCAACGAAGTTCGAATTCTATGTCAAGTGAGCCATAGAAGCCTAGTCAGATTGATGGGCTGTTGCCTTGAGCTTGAACAGCCACTTTTAATCTACGAGTACATTCCAAATGGTACCCTTTTCGAACATCTCCATGGAAAGTACAACACTGCTAATAGGGTCTCACTCACTTGGCGTATGAGGCTTACTATTGCTCATCAAACAGCTGAAGGACTTGCCTATCTCCACGATTCAGTCACGCCACGGATTTACCACCGCGACATAAAATCAAGCAACATTTTACTCGACAAAATGCTTAACTCTAAAGTTGCTGATTTTGGACTGTCAAGACTGGCCGTGGCCGAATCAAGTCACATTACAACATGTGCTCAGGGGTCCTTTGGATACCTAGACCCGGAGTACTACCTGAACTTTCAACTGACAGATAAGAGCGATGTTTATAGCTATGGAGTGGTGCTGTTGGAGCTCTTGACTTCTAAGAAAGCTATTGATTTTAACAGAGAAGAAGAAGATGTGAACTTGGTGGTTTATGTCAAGAACACTTTAAAAGAAGAGAGGCTAATGGATGCCGTAGATCCTAGGCTTAAAGAAGGGGCTGGCATCGTAGAGCTGGAGACTATGAAGGAATTCGCGTCTCTAGCCTTGGCGTGCTTGGATGAGCGAAGGCAGAACAGACCCTCCATGAAGGAAGTTGCTGATGAGATTCAGTACATCATTGGCATTGTTACAAATGAGGATTCAGCAACTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

621

Amino Acids

69.13

Weight (kDa)

8.04

Isoelectric Point (pI)

31.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	29 - 83	2.2e-06	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	330 - 602	8.4e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	331 - 604	4.4e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53840
malus_domestica	MD12G1149600.v1.1
prunus_persica	Prupe.6G261100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0465331 RchiOBHm_Chr3g0465341
rosa_laevigata	RLG00000024646
rosa_multiflora	Rmu_co8329097.1_g000001 Rmu_co8386781.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415120
rosa_rugosa	Rorug03G0076200 Rorug03G0076300
rosa_samantha	Rh3BG141100 Rh3CG142000 Rh3CG142100 Rh3DG142100 Rh3DG142200
rosa_wichuraiana	Rw3G011540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	511
Acc65I	GGTACC	1 cut(s)	1223
AccB1I	GGYRCC	3 cut(s)	109, 1007, 1223
AccI	GTMKAC	2 cut(s)	515, 840
AccII	CGCG	4 cut(s)	649, 852, 1368, 1742
AciI	CCGC	9 cut(s)	96, 119, 141, 260, 468, 474, 647, 649, 1366
AclI	AACGTT	1 cut(s)	279
AclWI	GGATC	2 cut(s)	1110, 1679
AcoI	YGGCCR	5 cut(s)	97, 257, 294, 1443, 1449
AcsI	RAATTY	4 cut(s)	982, 1107, 1125, 1736
AcuI	CTGAAG	1 cut(s)	1338
AcyI	GRCGYC	2 cut(s)	192, 244
AfaI	GTAC	6 cut(s)	55, 1211, 1225, 1253, 1511, 1820
AfiI	CCNNNNNNNGG	8 cut(s)	106, 140, 262, 474, 620, 897, 1083, 1504
AflIII	ACRYGT	2 cut(s)	906, 1472
AgsI	TTSAA	8 cut(s)	42, 343, 502, 604, 755, 1033, 1187, 1526
AhlI	ACTAGT	1 cut(s)	13
AjnI	CCWGG	1 cut(s)	87
AleI	CACNNNNGTG	1 cut(s)	107
AloI	GAACNNNNNNTCC	2 cut(s)	1772, 1804
Alw21I	GWGCWC	2 cut(s)	1480, 1578
Alw26I	GTCTC	3 cut(s)	1276, 1718, 1749
AlwI	GGATC	2 cut(s)	1110, 1679
Ama87I	CYCGRG	2 cut(s)	897, 1082
AoxI	GGCC	8 cut(s)	97, 257, 294, 313, 596, 611, 1443, 1449
ApeKI	GCWGC	5 cut(s)	84, 354, 461, 797, 872
ApoI	RAATTY	4 cut(s)	982, 1107, 1125, 1736
ArsI	GACNNNNNNTTYG	2 cut(s)	1117, 1149
Asp700I	GAANNNNTTC	1 cut(s)	1736
Asp718I	GGTACC	1 cut(s)	1223
AspA2I	CCTAGG	1 cut(s)	1688
AspS9I	GGNCC	3 cut(s)	596, 760, 1485
AsuC2I	CCSGG	7 cut(s)	241, 264, 898, 899, 1083, 1084, 1505
AsuHPI	GGTGA	3 cut(s)	123, 340, 1034
AsuII	TTCGAA	2 cut(s)	1123, 1233
AvaI	CYCGRG	2 cut(s)	897, 1082
AvaII	GGWCC	2 cut(s)	760, 1485
AvrII	CCTAGG	1 cut(s)	1688
BaeGI	GKGCMC	1 cut(s)	114
BanI	GGYRCC	3 cut(s)	109, 1007, 1223
BanII	GRGCYC	2 cut(s)	1078, 1578
BauI	CACGAG	1 cut(s)	967
BbsI	GAAGAC	2 cut(s)	909, 1021
Bbv12I	GWGCWC	2 cut(s)	1480, 1578
BbvI	GCAGC	5 cut(s)	71, 366, 448, 784, 884
BccI	CCATC	3 cut(s)	773, 1043, 1159
BceAI	ACGGC	3 cut(s)	785, 1430, 1664
BciT130I	CCWGG	1 cut(s)	89
BciVI	GTATCC	1 cut(s)	1487
BclI	TGATCA	1 cut(s)	789
BcnI	CCSGG	7 cut(s)	241, 264, 898, 899, 1083, 1084, 1505
BcoDI	GTCTC	3 cut(s)	1276, 1718, 1749
BcuI	ACTAGT	1 cut(s)	13
BfaI	CTAG	6 cut(s)	14, 1043, 1154, 1499, 1689, 1748
BfmI	CTRYAG	1 cut(s)	1062
BfuI	GTATCC	1 cut(s)	1487
BisI	GCNGC	8 cut(s)	85, 97, 260, 355, 462, 647, 798, 873
BlnI	CCTAGG	1 cut(s)	1688
BlpI	GCTNAGC	1 cut(s)	122
BlsI	GCNGC	8 cut(s)	86, 98, 261, 356, 463, 648, 799, 874
BmcAI	AGTACT	1 cut(s)	1511
Bme1390I	CCNGG	8 cut(s)	89, 241, 264, 898, 899, 1083, 1084, 1505
Bme18I	GGWCC	2 cut(s)	760, 1485
BmeT110I	CYCGRG	2 cut(s)	897, 1082
BmgT120I	GGNCC	3 cut(s)	596, 760, 1485
BmiI	GGNNCC	8 cut(s)	111, 654, 761, 762, 820, 1009, 1225, 1486
BmrFI	CCNGG	8 cut(s)	89, 241, 264, 898, 899, 1083, 1084, 1505
BmsI	GCATC	3 cut(s)	155, 1666, 1719
BpiI	GAAGAC	2 cut(s)	909, 1021
BpmI	CTGGAG	1 cut(s)	1742
Bpu10I	CCTNAGC	1 cut(s)	1479
Bpu1102I	GCTNAGC	1 cut(s)	122
Bpu14I	TTCGAA	2 cut(s)	1123, 1233
BpuEI	CTTGAG	1 cut(s)	1199
BpuMI	CCSGG	7 cut(s)	241, 264, 898, 899, 1083, 1084, 1505
BsaAI	YACGTR	1 cut(s)	285
BsaHI	GRCGYC	2 cut(s)	192, 244
BsaI	GGTCTC	1 cut(s)	1276
BsaWI	WCCGGW	2 cut(s)	106, 929
BsaXI	ACNNNNNCTCC	6 cut(s)	620, 644, 650, 674, 1772, 1802
Bsc4I	CCNNNNNNNGG	8 cut(s)	106, 140, 262, 474, 620, 897, 1083, 1504
Bse118I	RCCGGY	1 cut(s)	1010
Bse1I	ACTGG	3 cut(s)	511, 751, 1446
Bse3DI	GCAATG	3 cut(s)	462, 792, 813
BseBI	CCWGG	1 cut(s)	89
BseGI	GGATG	2 cut(s)	1681, 1771
BseLI	CCNNNNNNNGG	8 cut(s)	106, 140, 262, 474, 620, 897, 1083, 1504
BseMI	GCAATG	3 cut(s)	462, 792, 813
BseMII	CTCAG	2 cut(s)	606, 1493
BseNI	ACTGG	3 cut(s)	511, 751, 1446
BseSI	GKGCMC	1 cut(s)	114
BseX3I	CGGCCG	2 cut(s)	257, 294
BseXI	GCAGC	5 cut(s)	71, 366, 448, 784, 884
BseYI	CCCAGC	1 cut(s)	1077
Bsh1236I	CGCG	4 cut(s)	649, 852, 1368, 1742
Bsh1285I	CGRYCG	2 cut(s)	260, 297
BshFI	GGCC	8 cut(s)	99, 259, 296, 315, 598, 613, 1445, 1451
BshNI	GGYRCC	3 cut(s)	109, 1007, 1223
BsiEI	CGRYCG	2 cut(s)	260, 297
BsiHKAI	GWGCWC	2 cut(s)	1480, 1578
BsiHKCI	CYCGRG	2 cut(s)	897, 1082
BsiSI	CCGG	9 cut(s)	107, 241, 256, 263, 898, 930, 1011, 1083, 1505
BslFI	GGGAC	2 cut(s)	773, 880
BslI	CCNNNNNNNGG	8 cut(s)	106, 140, 262, 474, 620, 897, 1083, 1504
BsmAI	GTCTC	3 cut(s)	1276, 1718, 1749
BsmBI	CGTCTC	1 cut(s)	1749
BsmFI	GGGAC	2 cut(s)	773, 880
BsmI	GAATGC	1 cut(s)	830
BsnI	GGCC	8 cut(s)	99, 259, 296, 315, 598, 613, 1445, 1451
Bso31I	GGTCTC	1 cut(s)	1276
BsoBI	CYCGRG	2 cut(s)	897, 1082
Bsp119I	TTCGAA	2 cut(s)	1123, 1233
Bsp1286I	GDGCHC	4 cut(s)	114, 1078, 1480, 1578
Bsp1407I	TGTACA	1 cut(s)	53
Bsp143I	GATC	4 cut(s)	712, 789, 1102, 1684
Bsp1720I	GCTNAGC	1 cut(s)	122
Bsp19I	CCATGG	1 cut(s)	1243
BspACI	CCGC	9 cut(s)	96, 119, 141, 260, 468, 474, 647, 649, 1366
BspANI	GGCC	8 cut(s)	99, 259, 296, 315, 598, 613, 1445, 1451
BspCNI	CTCAG	2 cut(s)	607, 1492
BspFNI	CGCG	4 cut(s)	649, 852, 1368, 1742
BspLI	GGNNCC	8 cut(s)	111, 654, 761, 762, 820, 1009, 1225, 1486
BspMAI	CTGCAG	1 cut(s)	1066
BspPI	GGATC	2 cut(s)	1110, 1679
BspQI	GCTCTTC	1 cut(s)	953
BspT104I	TTCGAA	2 cut(s)	1123, 1233
BspT107I	GGYRCC	3 cut(s)	109, 1007, 1223
BspTNI	GGTCTC	1 cut(s)	1276
BsrDI	GCAATG	3 cut(s)	462, 792, 813
BsrFI	RCCGGY	1 cut(s)	1010
BsrGI	TGTACA	1 cut(s)	53
BsrI	ACTGG	3 cut(s)	511, 751, 1446
BssAI	RCCGGY	1 cut(s)	1010
BssMI	GATC	4 cut(s)	712, 789, 1102, 1684
BssNI	GRCGYC	2 cut(s)	192, 244
BssSI	CACGAG	1 cut(s)	967
BssT1I	CCWWGG	4 cut(s)	310, 1243, 1688, 1752
Bst2BI	CACGAG	1 cut(s)	967
Bst2UI	CCWGG	1 cut(s)	89
Bst4CI	ACNGT	7 cut(s)	37, 76, 183, 364, 380, 608, 1434
Bst6I	CTCTTC	3 cut(s)	27, 953, 1656
BstACI	GRCGYC	2 cut(s)	192, 244
BstAPI	GCANNNNNTGC	1 cut(s)	461
BstAUI	TGTACA	1 cut(s)	53
BstBAI	YACGTR	1 cut(s)	285
BstBI	TTCGAA	2 cut(s)	1123, 1233
BstC8I	GCNNGC	3 cut(s)	472, 1708, 1759
BstDEI	CTNAG	5 cut(s)	67, 122, 615, 1479, 1587
BstDSI	CCRYGG	4 cut(s)	647, 1243, 1352, 1446
BstF5I	GGATG	2 cut(s)	1681, 1771
BstFNI	CGCG	4 cut(s)	649, 852, 1368, 1742
BstKTI	GATC	4 cut(s)	715, 792, 1105, 1687
BstMAI	GTCTC	3 cut(s)	1276, 1718, 1749
BstMBI	GATC	4 cut(s)	712, 789, 1102, 1684
BstMCI	CGRYCG	2 cut(s)	260, 297
BstNI	CCWGG	1 cut(s)	89
BstNSI	RCATGY	3 cut(s)	292, 910, 1476
BstSCI	CCNGG	8 cut(s)	87, 239, 262, 896, 897, 1081, 1082, 1503
BstSFI	CTRYAG	1 cut(s)	1062
BstSLI	GKGCMC	1 cut(s)	114
BstUI	CGCG	4 cut(s)	649, 852, 1368, 1742
BstV1I	GCAGC	5 cut(s)	71, 366, 448, 784, 884
BstV2I	GAAGAC	2 cut(s)	909, 1021
BstX2I	RGATCY	1 cut(s)	1684
BstYI	RGATCY	1 cut(s)	1684
BstZI	CGGCCG	2 cut(s)	257, 294
BsuI	GTATCC	1 cut(s)	1487
BsuRI	GGCC	8 cut(s)	99, 259, 296, 315, 598, 613, 1445, 1451
BtgI	CCRYGG	4 cut(s)	647, 1243, 1352, 1446
BtgZI	GCGATG	1 cut(s)	1557
BtsCI	GGATG	2 cut(s)	1681, 1771
BtsI	GCAGTG	2 cut(s)	1059, 1257
BtsIMutI	CAGTG	4 cut(s)	179, 369, 1059, 1257
Cac8I	GCNNGC	3 cut(s)	472, 1708, 1759
Cfr10I	RCCGGY	1 cut(s)	1010
Cfr13I	GGNCC	3 cut(s)	596, 760, 1485
Cfr42I	CCGCGG	1 cut(s)	650
Cfr9I	CCCGGG	2 cut(s)	897, 1082
CseI	GACGC	4 cut(s)	200, 233, 858, 1731
Csp6I	GTAC	6 cut(s)	54, 1210, 1224, 1252, 1510, 1819
CspCI	CAANNNNNGTGG	2 cut(s)	490, 525
CviAII	CATG	8 cut(s)	170, 289, 373, 907, 1099, 1244, 1473, 1792
CviQI	GTAC	6 cut(s)	54, 1210, 1224, 1252, 1510, 1819
DdeI	CTNAG	5 cut(s)	67, 122, 615, 1479, 1587
DpnI	GATC	4 cut(s)	714, 791, 1104, 1686
DpnII	GATC	4 cut(s)	712, 789, 1102, 1684
DraI	TTTAAA	2 cut(s)	304, 1656
DrdI	GACNNNNNNGTC	1 cut(s)	511
DseDI	GACNNNNNNGTC	1 cut(s)	511
EaeI	YGGCCR	5 cut(s)	97, 257, 294, 1443, 1449
EagI	CGGCCG	2 cut(s)	257, 294
Eam1104I	CTCTTC	3 cut(s)	27, 953, 1656
EarI	CTCTTC	3 cut(s)	27, 953, 1656
Ecl136II	GAGCTC	1 cut(s)	1576
EclXI	CGGCCG	2 cut(s)	257, 294
Eco130I	CCWWGG	4 cut(s)	310, 1243, 1688, 1752
Eco147I	AGGCCT	1 cut(s)	315
Eco24I	GRGCYC	2 cut(s)	1078, 1578
Eco31I	GGTCTC	1 cut(s)	1276
Eco47I	GGWCC	2 cut(s)	760, 1485
Eco52I	CGGCCG	2 cut(s)	257, 294
Eco53kI	GAGCTC	1 cut(s)	1576
Eco57I	CTGAAG	1 cut(s)	1338
Eco88I	CYCGRG	2 cut(s)	897, 1082
EcoICRI	GAGCTC	1 cut(s)	1576
EcoO109I	RGGNCCY	2 cut(s)	760, 1485
EcoRI	GAATTC	2 cut(s)	1125, 1736
EcoRII	CCWGG	1 cut(s)	87
EcoT14I	CCWWGG	4 cut(s)	310, 1243, 1688, 1752
EcoT38I	GRGCYC	2 cut(s)	1078, 1578
ErhI	CCWWGG	4 cut(s)	310, 1243, 1688, 1752
Esp3I	CGTCTC	1 cut(s)	1749
FaeI	CATG	8 cut(s)	173, 292, 376, 910, 1102, 1247, 1476, 1795
FaqI	GGGAC	2 cut(s)	773, 880
FatI	CATG	8 cut(s)	169, 288, 372, 906, 1098, 1243, 1472, 1791
FauI	CCCGC	3 cut(s)	126, 467, 642
FbaI	TGATCA	1 cut(s)	789
FblI	GTMKAC	2 cut(s)	515, 840
Fnu4HI	GCNGC	8 cut(s)	85, 97, 260, 355, 462, 647, 798, 873
FokI	GGATG	2 cut(s)	1688, 1778
FriOI	GRGCYC	2 cut(s)	1078, 1578
Fsp4HI	GCNGC	8 cut(s)	85, 97, 260, 355, 462, 647, 798, 873
FspBI	CTAG	6 cut(s)	14, 1043, 1154, 1499, 1689, 1748
GluI	GCNGC	8 cut(s)	85, 97, 260, 355, 462, 647, 798, 873
GsaI	CCCAGC	1 cut(s)	1081
GsuI	CTGGAG	1 cut(s)	1742
HaeIII	GGCC	8 cut(s)	99, 259, 296, 315, 598, 613, 1445, 1451
HapII	CCGG	9 cut(s)	107, 241, 256, 263, 898, 930, 1011, 1083, 1505
HgaI	GACGC	4 cut(s)	200, 233, 858, 1731
Hin1I	GRCGYC	2 cut(s)	192, 244
Hin1II	CATG	8 cut(s)	173, 292, 376, 910, 1102, 1247, 1476, 1795
HindIII	AAGCTT	1 cut(s)	784
HinfI	GANTC	7 cut(s)	584, 617, 641, 1340, 1454, 1813, 1847
HpaII	CCGG	9 cut(s)	107, 241, 256, 263, 898, 930, 1011, 1083, 1505
HphI	GGTGA	3 cut(s)	123, 340, 1034
Hpy166II	GTNNAC	4 cut(s)	516, 722, 841, 1627
Hpy188I	TCNGA	4 cut(s)	164, 700, 717, 1160
Hpy188III	TCNNGA	8 cut(s)	403, 484, 502, 967, 1043, 1437, 1579, 1645
Hpy8I	GTNNAC	4 cut(s)	516, 722, 841, 1627
Hpy99I	CGWCG	1 cut(s)	852
HpyAV	CCTTC	7 cut(s)	538, 723, 1313, 1694, 1726, 1767, 1789
HpyCH4III	ACNGT	7 cut(s)	37, 76, 183, 364, 380, 608, 1434
HpyCH4IV	ACGT	2 cut(s)	279, 284
HpyCH4V	TGCA	9 cut(s)	173, 288, 663, 741, 773, 806, 830, 917, 1064
HpyF3I	CTNAG	5 cut(s)	67, 122, 615, 1479, 1587
HpySE526I	ACGT	2 cut(s)	279, 284
Hsp92I	GRCGYC	2 cut(s)	192, 244
Hsp92II	CATG	8 cut(s)	173, 292, 376, 910, 1102, 1247, 1476, 1795
KflI	GGGWCCC	1 cut(s)	760
KpnI	GGTACC	1 cut(s)	1227
Ksp22I	TGATCA	1 cut(s)	789
KspI	CCGCGG	1 cut(s)	650
Kzo9I	GATC	4 cut(s)	712, 789, 1102, 1684
LguI	GCTCTTC	1 cut(s)	953
LmnI	GCTCC	3 cut(s)	652, 818, 1573
Lsp1109I	GCAGC	5 cut(s)	71, 366, 448, 784, 884
LweI	GCATC	3 cut(s)	155, 1666, 1719
MaeI	CTAG	6 cut(s)	14, 1043, 1154, 1499, 1689, 1748
MaeII	ACGT	2 cut(s)	279, 284
MaeIII	GTNAC	5 cut(s)	177, 280, 1345, 1460, 1834
MalI	GATC	4 cut(s)	714, 791, 1104, 1686
MboI	GATC	4 cut(s)	712, 789, 1102, 1684
MboII	GAAGA	8 cut(s)	14, 914, 940, 1021, 1625, 1628, 1631, 1673
MflI	RGATCY	1 cut(s)	1684
MhlI	GDGCHC	4 cut(s)	114, 1078, 1480, 1578
MluCI	AATT	9 cut(s)	225, 249, 531, 577, 861, 982, 1107, 1125, 1736
MlyI	GAGTC	2 cut(s)	578, 626
MmeI	TCCRAC	1 cut(s)	1551
MnlI	CCTC	9 cut(s)	206, 344, 475, 832, 1018, 1284, 1659, 1798, 1837
MroXI	GAANNNNTTC	1 cut(s)	1736
MslI	CAYNNNNRTG	2 cut(s)	107, 1242
MspA1I	CMGCKG	2 cut(s)	649, 1316
MspI	CCGG	9 cut(s)	107, 241, 256, 263, 898, 930, 1011, 1083, 1505
MspR9I	CCNGG	8 cut(s)	89, 241, 264, 898, 899, 1083, 1084, 1505
Mva1269I	GAATGC	1 cut(s)	830
MvaI	CCWGG	1 cut(s)	89
MvnI	CGCG	4 cut(s)	649, 852, 1368, 1742
NciI	CCSGG	7 cut(s)	241, 264, 898, 899, 1083, 1084, 1505
NcoI	CCATGG	1 cut(s)	1243
NdeII	GATC	4 cut(s)	712, 789, 1102, 1684
NlaIII	CATG	8 cut(s)	173, 292, 376, 910, 1102, 1247, 1476, 1795
NlaIV	GGNNCC	8 cut(s)	111, 654, 761, 762, 820, 1009, 1225, 1486
NmuCI	GTSAC	3 cut(s)	177, 1345, 1460
NspI	RCATGY	3 cut(s)	292, 910, 1476
NspV	TTCGAA	2 cut(s)	1123, 1233
OliI	CACNNNNGTG	1 cut(s)	107
PceI	AGGCCT	1 cut(s)	315
PciI	ACATGT	2 cut(s)	906, 1472
PciSI	GCTCTTC	1 cut(s)	953
PctI	GAATGC	1 cut(s)	830
PdmI	GAANNNNTTC	1 cut(s)	1736
PfeI	GAWTC	5 cut(s)	641, 1340, 1454, 1813, 1847
PflFI	GACNNNGTC	1 cut(s)	892
PkrI	GCNGC	8 cut(s)	86, 98, 261, 356, 463, 648, 799, 874
PleI	GAGTC	2 cut(s)	578, 625
PpsI	GAGTC	2 cut(s)	578, 625
Ppu21I	YACGTR	1 cut(s)	285
PpuMI	RGGWCCY	2 cut(s)	760, 1485
PscI	ACATGT	2 cut(s)	906, 1472
Psp124BI	GAGCTC	1 cut(s)	1578
Psp1406I	AACGTT	1 cut(s)	279
Psp5II	RGGWCCY	2 cut(s)	760, 1485
Psp6I	CCWGG	1 cut(s)	87
PspFI	CCCAGC	1 cut(s)	1077
PspGI	CCWGG	1 cut(s)	87
PspN4I	GGNNCC	8 cut(s)	111, 654, 761, 762, 820, 1009, 1225, 1486
PspPI	GGNCC	3 cut(s)	596, 760, 1485
PspPPI	RGGWCCY	2 cut(s)	760, 1485
PstI	CTGCAG	1 cut(s)	1066
PsuI	RGATCY	1 cut(s)	1684
PsyI	GACNNNGTC	1 cut(s)	892
PvuII	CAGCTG	1 cut(s)	1316
RsaI	GTAC	6 cut(s)	55, 1211, 1225, 1253, 1511, 1820
RsaNI	GTAC	6 cut(s)	54, 1210, 1224, 1252, 1510, 1819
RseI	CAYNNNNRTG	2 cut(s)	107, 1242
SacI	GAGCTC	1 cut(s)	1578
SacII	CCGCGG	1 cut(s)	650
SapI	GCTCTTC	1 cut(s)	953
SatI	GCNGC	8 cut(s)	85, 97, 260, 355, 462, 647, 798, 873
Sau3AI	GATC	4 cut(s)	712, 789, 1102, 1684
Sau96I	GGNCC	3 cut(s)	596, 760, 1485
ScaI	AGTACT	1 cut(s)	1511
SchI	GAGTC	2 cut(s)	578, 626
ScrFI	CCNGG	8 cut(s)	89, 241, 264, 898, 899, 1083, 1084, 1505
SduI	GDGCHC	4 cut(s)	114, 1078, 1480, 1578
SfaNI	GCATC	3 cut(s)	155, 1666, 1719
SfcI	CTRYAG	1 cut(s)	1062
Sfr303I	CCGCGG	1 cut(s)	650
SfuI	TTCGAA	2 cut(s)	1123, 1233
SgrBI	CCGCGG	1 cut(s)	650
SinI	GGWCC	2 cut(s)	760, 1485
SmaI	CCCGGG	2 cut(s)	899, 1084
SmiMI	CAYNNNNRTG	2 cut(s)	107, 1242
SmlI	CTYRAG	1 cut(s)	1178
SmoI	CTYRAG	1 cut(s)	1178
SpeI	ACTAGT	1 cut(s)	13
Sse9I	AATT	9 cut(s)	225, 249, 531, 577, 861, 982, 1107, 1125, 1736
SseBI	AGGCCT	1 cut(s)	315
SsiI	CCGC	9 cut(s)	96, 119, 141, 260, 468, 474, 647, 649, 1366
SspMI	CTAG	6 cut(s)	14, 1043, 1154, 1499, 1689, 1748
SstI	GAGCTC	1 cut(s)	1578
StuI	AGGCCT	1 cut(s)	315
StyD4I	CCNGG	8 cut(s)	87, 239, 262, 896, 897, 1081, 1082, 1503
StyI	CCWWGG	4 cut(s)	310, 1243, 1688, 1752
TaaI	ACNGT	7 cut(s)	37, 76, 183, 364, 380, 608, 1434
TaiI	ACGT	2 cut(s)	282, 287
TaqI	TCGA	5 cut(s)	404, 781, 1123, 1233, 1395
TasI	AATT	9 cut(s)	225, 249, 531, 577, 861, 982, 1107, 1125, 1736
TatI	WGTACW	5 cut(s)	53, 1209, 1251, 1509, 1818
TauI	GCSGC	3 cut(s)	99, 262, 649
TfiI	GAWTC	5 cut(s)	641, 1340, 1454, 1813, 1847
TscAI	CASTG	4 cut(s)	186, 369, 1066, 1264
TseFI	GTSAC	3 cut(s)	177, 1345, 1460
TseI	GCWGC	5 cut(s)	84, 354, 461, 797, 872
Tsp45I	GTSAC	3 cut(s)	177, 1345, 1460
TspDTI	ATGAA	6 cut(s)	389, 402, 557, 823, 1745, 1808
TspGWI	ACGGA	1 cut(s)	1369
TspMI	CCCGGG	2 cut(s)	897, 1082
TspRI	CASTG	4 cut(s)	186, 369, 1066, 1264
Tth111I	GACNNNGTC	1 cut(s)	892
VpaK11BI	GGWCC	2 cut(s)	760, 1485
XapI	RAATTY	4 cut(s)	982, 1107, 1125, 1736
XbaI	TCTAGA	1 cut(s)	1042
XceI	RCATGY	3 cut(s)	292, 910, 1476
XmaI	CCCGGG	2 cut(s)	897, 1082
XmaJI	CCTAGG	1 cut(s)	1688
XmiI	GTMKAC	2 cut(s)	515, 840
XmnI	GAANNNNTTC	1 cut(s)	1736
XspI	CTAG	6 cut(s)	14, 1043, 1154, 1499, 1689, 1748
ZrmI	AGTACT	1 cut(s)	1511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.