Rh3BG141100

Wall-associated receptor kinase-like 20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013898
Physical Location & Seq
Reverse (-)
11941222 .. 11943882
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG141100.1

Sequence Viewer

Length: 1287 bp
ATGTTATGGTGCATAACCGCCAGCCGTTGTCCGGATTGTGGCCGCACCACCGTGCCGTATCCACTGAGCACGGAGCCAAACTGCGGTGACCAGGCAAACAAGGTTCAGTGCAATGCAGGAACACTGTGGTTCCACACCCTCAACAACTCCTATCCAGTCACATCCGTTAATCCGCTAACGCAGCGGATTGTGATCCAACCACCAGGAGTGGCAAACAAGAACACATGTGTCGCTGCTGATTTCCAATCTCAGGGAATTTTGCTGGACAACAACCTTCCCTTCAACATTAGTGGCAGCAACACTGTGATAGGCATGAACTGTTCAACTGAAATGCTTACCACCTCCTTAAACTGCTCCTCCAATAGCATATGCCACGACTACGTTAGGCACAATCCAATGGCATCATCAGCTTGTGGGAACTTGTCATTATGTTGTTCATACAAAACTGGTGGATCTTCAAATGCCTACAAAATCAGGGTTAGAAAGGAACGTTGTGTTGCCTATGTGAGTTTTGTGAATTTGGACCCTACGTTGGATGTGAGCAAGTGGCCTCAGCCTGCGGTTGAGATGCAATGGGTGTTGCCGGAAGAGCCAGGATGGGCAAACATAAAGTGTAAAAAGTTGAGAGGATGCAAACGGAAAAAGTTTGTAGCCCCAGTCATTGGAGGCTCCGCCTTTGCTGTTGTTGCAATGCTAATTGGAGGATCCTCTGCCTTTATAATTAAGCGACGTGGTCGAAACGTTGCAAGATCAGCAAAGTCTAGCTTGGCAAAGTTCAGAGAAGACATACTAAATGCTAACAGTAGTACTGGCAAATCAGCCAAGAATTTCACAGGCAAAGAAATCACAAAAGCAACCAACAACTTCTCCAAAGACAACCTTCTTGGCTCCGGTGGCTTTGGCGATGTCTTCAAGGCCACACTTGACGATGGAACTGTCACAGCAGTGAAGAAAGCCAAGGCTGGAAACATAAAGGGCATCGATCAAATTCTCAACGAAGTTCGAATTCTTTGCCAAAAAGCTATTGATTTTAACAGACAAGAAGACGACGTGAACTTGGTGGTTTATGTCGAGAACATTTTAAGGGAAGACAGACTAAATGATGCCATTGAACCTAAGCTTAAAGAGGAGGCTGGCACAATGGCTCTGGAGACTATGAAGGCATTAGGGTCTCTGGCAGCTGCATGCTTGGATGAGAGGAGGCAAAATCGACCCTCCATGAAAGAAGTTGCTGATGAAATTGAGTACATTATTAGTATCGTTACAAATGAGGTTTCAGCAACTTAA

Protein Analysis

428

Amino Acids

46.57

Weight (kDa)

8.81

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 10 - 65 2.2e-06 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53840
malus_domestica MD12G1149600.v1.1
prunus_persica Prupe.6G261100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0465331 RchiOBHm_Chr3g0465341
rosa_laevigata RLG00000024646
rosa_multiflora Rmu_co8329097.1_g000001 Rmu_co8386781.1_g000001
rosa_roxburghii Rroxscaffold_6G00415120
rosa_rugosa Rorug03G0076200 Rorug03G0076300
rosa_samantha Rh3BG141100 Rh3CG142000 Rh3CG142100 Rh3DG142100 Rh3DG142200
rosa_wichuraiana Rw3G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 719
AccB7I CCANNNNNTGG 1 cut(s) 662
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 7 cut(s) 18, 43, 84, 173, 184, 560, 672
AclI AACGTT 2 cut(s) 490, 741
AclWI GGATC 4 cut(s) 187, 460, 699, 712
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 5 cut(s) 255, 517, 826, 987, 1005
AfaI GTAC 2 cut(s) 808, 1247
AfiI CCNNNNNNNGG 6 cut(s) 31, 38, 83, 250, 532, 662
AflIII ACRYGT 1 cut(s) 224
AgsI TTSAA 5 cut(s) 283, 324, 459, 913, 1112
AjiI CACGTC 2 cut(s) 731, 1051
AjnI CCWGG 3 cut(s) 90, 202, 592
AleI CACNNNNGTG 2 cut(s) 50, 944
AluBI AGCT 5 cut(s) 410, 765, 1022, 1120, 1181
AluI AGCT 5 cut(s) 410, 765, 1022, 1120, 1181
Alw21I GWGCWC 1 cut(s) 71
Alw26I GTCTC 2 cut(s) 1145, 1176
AlwI GGATC 4 cut(s) 187, 460, 699, 712
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 3 cut(s) 40, 548, 915
ApeKI GCWGC 5 cut(s) 181, 233, 294, 1178, 1181
ApoI RAATTY 5 cut(s) 255, 517, 826, 987, 1005
AspS9I GGNCC 1 cut(s) 523
AsuHPI GGTGA 1 cut(s) 98
AsuII TTCGAA 1 cut(s) 1003
AvaII GGWCC 1 cut(s) 523
BamHI GGATCC 1 cut(s) 704
BbsI GAAGAC 4 cut(s) 789, 901, 1050, 1095
Bbv12I GWGCWC 1 cut(s) 71
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 5 cut(s) 193, 220, 306, 1168, 1190
BccI CCATC 2 cut(s) 591, 923
BceAI ACGGC 2 cut(s) 9, 40
BcgI CGANNNNNNTGC 2 cut(s) 993, 1027
BciT130I CCWGG 3 cut(s) 92, 204, 594
BciVI GTATCC 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 1145, 1176
BfaI CTAG 1 cut(s) 762
BfuI GTATCC 1 cut(s) 69
BisI GCNGC 6 cut(s) 43, 182, 234, 295, 1179, 1182
BlsI GCNGC 6 cut(s) 44, 183, 235, 296, 1180, 1183
BmcAI AGTACT 1 cut(s) 808
Bme1390I CCNGG 3 cut(s) 92, 204, 594
Bme18I GGWCC 1 cut(s) 523
BmgBI CACGTC 2 cut(s) 731, 1051
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 6 cut(s) 75, 131, 525, 670, 706, 889
BmrFI CCNGG 3 cut(s) 92, 204, 594
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 5 cut(s) 410, 558, 620, 987, 1093
BmuI ACTGGG 1 cut(s) 650
BpiI GAAGAC 4 cut(s) 789, 901, 1050, 1095
BpmI CTGGAG 1 cut(s) 1169
Bpu10I CCTNAGC 2 cut(s) 552, 1116
Bpu14I TTCGAA 1 cut(s) 1003
Bsa29I ATCGAT 1 cut(s) 981
BsaBI GATNNNNATC 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 1176
BsaJI CCNNGG 1 cut(s) 957
BsaWI WCCGGW 2 cut(s) 31, 890
BsaXI ACNNNNNCTCC 4 cut(s) 341, 371, 851, 881
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 38, 83, 250, 532, 662
Bse1I ACTGG 4 cut(s) 155, 451, 656, 814
Bse3DI GCAATG 3 cut(s) 118, 578, 696
Bse8I GATNNNNATC 1 cut(s) 191
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 3 cut(s) 92, 204, 594
BseCI ATCGAT 1 cut(s) 981
BseDI CCNNGG 1 cut(s) 957
BseGI GGATG 5 cut(s) 161, 541, 602, 635, 1198
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 6 cut(s) 31, 38, 83, 250, 532, 662
BseMI GCAATG 3 cut(s) 118, 578, 696
BseMII CTCAG 3 cut(s) 56, 263, 566
BseNI ACTGG 4 cut(s) 155, 451, 656, 814
BseRI GAGGAG 3 cut(s) 346, 1142, 1213
BseXI GCAGC 5 cut(s) 193, 220, 306, 1168, 1190
BshFI GGCC 3 cut(s) 42, 550, 917
BshVI ATCGAT 1 cut(s) 981
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 3 cut(s) 32, 584, 891
BslI CCNNNNNNNGG 6 cut(s) 31, 38, 83, 250, 532, 662
BsmAI GTCTC 2 cut(s) 1145, 1176
BsnI GGCC 3 cut(s) 42, 550, 917
Bso31I GGTCTC 1 cut(s) 1176
Bsp119I TTCGAA 1 cut(s) 1003
Bsp1286I GDGCHC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 5 cut(s) 192, 452, 704, 749, 982
BspACI CCGC 7 cut(s) 18, 43, 84, 173, 184, 560, 672
BspANI GGCC 3 cut(s) 42, 550, 917
BspCNI CTCAG 3 cut(s) 57, 262, 565
BspDI ATCGAT 1 cut(s) 981
BspEI TCCGGA 1 cut(s) 31
BspLI GGNNCC 6 cut(s) 75, 131, 525, 670, 706, 889
BspPI GGATC 4 cut(s) 187, 460, 699, 712
BspQI GCTCTTC 1 cut(s) 582
BspT104I TTCGAA 1 cut(s) 1003
BspTNI GGTCTC 1 cut(s) 1176
BsrDI GCAATG 3 cut(s) 118, 578, 696
BsrI ACTGG 4 cut(s) 155, 451, 656, 814
BssECI CCNNGG 1 cut(s) 957
BssMI GATC 5 cut(s) 192, 452, 704, 749, 982
BssT1I CCWWGG 1 cut(s) 957
Bst2UI CCWGG 3 cut(s) 92, 204, 594
Bst4CI ACNGT 6 cut(s) 52, 126, 304, 320, 803, 937
Bst6I CTCTTC 1 cut(s) 582
BstBI TTCGAA 1 cut(s) 1003
BstC8I GCNNGC 4 cut(s) 22, 558, 1135, 1186
BstDEI CTNAG 4 cut(s) 65, 249, 552, 1116
BstEII GGTNACC 1 cut(s) 86
BstF5I GGATG 5 cut(s) 161, 541, 602, 635, 1198
BstKTI GATC 5 cut(s) 195, 455, 707, 752, 985
BstMAI GTCTC 2 cut(s) 1145, 1176
BstMBI GATC 5 cut(s) 192, 452, 704, 749, 982
BstMWI GCNNNNNNNGC 6 cut(s) 181, 407, 589, 686, 752, 894
BstNI CCWGG 3 cut(s) 92, 204, 594
BstNSI RCATGY 2 cut(s) 228, 1188
BstPI GGTNACC 1 cut(s) 86
BstSCI CCNGG 3 cut(s) 90, 202, 592
BstV1I GCAGC 5 cut(s) 193, 220, 306, 1168, 1190
BstV2I GAAGAC 4 cut(s) 789, 901, 1050, 1095
BstX2I RGATCY 2 cut(s) 452, 704
BstYI RGATCY 2 cut(s) 452, 704
Bsu15I ATCGAT 1 cut(s) 981
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 3 cut(s) 42, 550, 917
BsuTUI ATCGAT 1 cut(s) 981
BtgZI GCGATG 1 cut(s) 918
BtrI CACGTC 2 cut(s) 731, 1051
BtsCI GGATG 5 cut(s) 161, 541, 602, 635, 1198
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 5 cut(s) 62, 113, 122, 300, 951
Cac8I GCNNGC 4 cut(s) 22, 558, 1135, 1186
Cfr13I GGNCC 1 cut(s) 523
ClaI ATCGAT 1 cut(s) 981
Csp6I GTAC 2 cut(s) 807, 1246
CspCI CAANNNNNGTGG 4 cut(s) 271, 306, 430, 465
CviAII CATG 4 cut(s) 225, 313, 1185, 1219
CviQI GTAC 2 cut(s) 807, 1246
DdeI CTNAG 4 cut(s) 65, 249, 552, 1116
DpnI GATC 5 cut(s) 194, 454, 706, 751, 984
DpnII GATC 5 cut(s) 192, 452, 704, 749, 982
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 661
Eco130I CCWWGG 1 cut(s) 957
Eco31I GGTCTC 1 cut(s) 1176
Eco47I GGWCC 1 cut(s) 523
Eco91I GGTNACC 1 cut(s) 86
EcoO65I GGTNACC 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 1005
EcoRII CCWGG 3 cut(s) 90, 202, 592
EcoT14I CCWWGG 1 cut(s) 957
ErhI CCWWGG 1 cut(s) 957
FaeI CATG 4 cut(s) 228, 316, 1188, 1222
FalI AAGNNNNNCTT 4 cut(s) 749, 781, 864, 896
FatI CATG 4 cut(s) 224, 312, 1184, 1218
FauNDI CATATG 1 cut(s) 368
Fnu4HI GCNGC 6 cut(s) 43, 182, 234, 295, 1179, 1182
FokI GGATG 5 cut(s) 148, 548, 609, 642, 1205
Fsp4HI GCNGC 6 cut(s) 43, 182, 234, 295, 1179, 1182
FspBI CTAG 1 cut(s) 762
GluI GCNGC 6 cut(s) 43, 182, 234, 295, 1179, 1182
GsuI CTGGAG 1 cut(s) 1169
HaeIII GGCC 3 cut(s) 42, 550, 917
HapII CCGG 3 cut(s) 32, 584, 891
Hin1II CATG 4 cut(s) 228, 316, 1188, 1222
HindIII AAGCTT 1 cut(s) 1118
HpaII CCGG 3 cut(s) 32, 584, 891
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 1054
Hpy188I TCNGA 1 cut(s) 779
Hpy188III TCNNGA 3 cut(s) 32, 1072, 1148
Hpy8I GTNNAC 1 cut(s) 1054
Hpy99I CGWCG 2 cut(s) 732, 1052
HpyAV CCTTC 4 cut(s) 284, 289, 890, 1153
HpyCH4III ACNGT 6 cut(s) 52, 126, 304, 320, 803, 937
HpyCH4IV ACGT 6 cut(s) 381, 490, 530, 730, 741, 1050
HpyCH4V TGCA 8 cut(s) 12, 111, 116, 571, 633, 689, 746, 1184
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 407, 589, 686, 752, 894
HpyF3I CTNAG 4 cut(s) 65, 249, 552, 1116
HpySE526I ACGT 6 cut(s) 381, 490, 530, 730, 741, 1050
Hsp92II CATG 4 cut(s) 228, 316, 1188, 1222
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 5 cut(s) 192, 452, 704, 749, 982
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 4 cut(s) 73, 359, 674, 893
Lsp1109I GCAGC 5 cut(s) 193, 220, 306, 1168, 1190
LweI GCATC 5 cut(s) 410, 558, 620, 987, 1093
MaeI CTAG 1 cut(s) 762
MaeII ACGT 6 cut(s) 381, 490, 530, 730, 741, 1050
MaeIII GTNAC 4 cut(s) 86, 157, 937, 1261
MalI GATC 5 cut(s) 194, 454, 706, 751, 984
MboI GATC 5 cut(s) 192, 452, 704, 749, 982
MboII GAAGA 7 cut(s) 447, 599, 794, 901, 961, 1055, 1100
MflI RGATCY 2 cut(s) 452, 704
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 8 cut(s) 255, 517, 696, 720, 826, 987, 1005, 1239
MmeI TCCRAC 2 cut(s) 220, 513
MroI TCCGGA 1 cut(s) 31
MseI TTAA 7 cut(s) 168, 347, 723, 1032, 1082, 1122, 1285
MslI CAYNNNNRTG 2 cut(s) 50, 944
MspA1I CMGCKG 2 cut(s) 184, 1181
MspI CCGG 3 cut(s) 32, 584, 891
MspR9I CCNGG 3 cut(s) 92, 204, 594
MvaI CCWGG 3 cut(s) 92, 204, 594
MwoI GCNNNNNNNGC 6 cut(s) 181, 407, 589, 686, 752, 894
NdeI CATATG 1 cut(s) 368
NdeII GATC 5 cut(s) 192, 452, 704, 749, 982
NlaIII CATG 4 cut(s) 228, 316, 1188, 1222
NlaIV GGNNCC 6 cut(s) 75, 131, 525, 670, 706, 889
NmuCI GTSAC 3 cut(s) 86, 157, 937
NspI RCATGY 2 cut(s) 228, 1188
NspV TTCGAA 1 cut(s) 1003
OliI CACNNNNGTG 2 cut(s) 50, 944
PaeI GCATGC 1 cut(s) 1188
PciI ACATGT 1 cut(s) 224
PciSI GCTCTTC 1 cut(s) 582
PflFI GACNNNGTC 1 cut(s) 732
PflMI CCANNNNNTGG 1 cut(s) 662
PkrI GCNGC 6 cut(s) 44, 183, 235, 296, 1180, 1183
PscI ACATGT 1 cut(s) 224
PsiI TTATAA 1 cut(s) 719
Psp1406I AACGTT 2 cut(s) 490, 741
Psp6I CCWGG 3 cut(s) 90, 202, 592
PspEI GGTNACC 1 cut(s) 86
PspGI CCWGG 3 cut(s) 90, 202, 592
PspN4I GGNNCC 6 cut(s) 75, 131, 525, 670, 706, 889
PspPI GGNCC 1 cut(s) 523
PsuI RGATCY 2 cut(s) 452, 704
PsyI GACNNNGTC 1 cut(s) 732
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 2 cut(s) 808, 1247
RsaNI GTAC 2 cut(s) 807, 1246
RseI CAYNNNNRTG 2 cut(s) 50, 944
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 7 cut(s) 168, 347, 723, 1032, 1082, 1122, 1285
SatI GCNGC 6 cut(s) 43, 182, 234, 295, 1179, 1182
Sau3AI GATC 5 cut(s) 192, 452, 704, 749, 982
Sau96I GGNCC 1 cut(s) 523
ScaI AGTACT 1 cut(s) 808
ScrFI CCNGG 3 cut(s) 92, 204, 594
SduI GDGCHC 1 cut(s) 71
SfaNI GCATC 5 cut(s) 410, 558, 620, 987, 1093
SfuI TTCGAA 1 cut(s) 1003
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 2 cut(s) 50, 944
SphI GCATGC 1 cut(s) 1188
Sse9I AATT 8 cut(s) 255, 517, 696, 720, 826, 987, 1005, 1239
SsiI CCGC 7 cut(s) 18, 43, 84, 173, 184, 560, 672
SspMI CTAG 1 cut(s) 762
StyD4I CCNGG 3 cut(s) 90, 202, 592
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 6 cut(s) 52, 126, 304, 320, 803, 937
TaiI ACGT 6 cut(s) 384, 493, 533, 733, 744, 1053
TaqI TCGA 5 cut(s) 736, 981, 1003, 1071, 1210
TasI AATT 8 cut(s) 255, 517, 696, 720, 826, 987, 1005, 1239
TatI WGTACW 2 cut(s) 806, 1245
TauI GCSGC 1 cut(s) 45
Tru1I TTAA 7 cut(s) 168, 347, 723, 1032, 1082, 1122, 1285
Tru9I TTAA 7 cut(s) 168, 347, 723, 1032, 1082, 1122, 1285
TscAI CASTG 5 cut(s) 69, 113, 129, 307, 951
TseFI GTSAC 3 cut(s) 86, 157, 937
TseI GCWGC 5 cut(s) 181, 233, 294, 1178, 1181
Tsp45I GTSAC 3 cut(s) 86, 157, 937
TspDTI ATGAA 5 cut(s) 329, 426, 1172, 1235, 1251
TspGWI ACGGA 3 cut(s) 86, 154, 652
TspRI CASTG 5 cut(s) 69, 113, 129, 307, 951
Tth111I GACNNNGTC 1 cut(s) 732
Van91I CCANNNNNTGG 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 523
XapI RAATTY 5 cut(s) 255, 517, 826, 987, 1005
XceI RCATGY 2 cut(s) 228, 1188
XspI CTAG 1 cut(s) 762
ZrmI AGTACT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.