RchiOBHm_Chr3g0465341

Wall-associated receptor kinase-like 20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013898
Physical Location & Seq
Reverse (-)
12134556 .. 12135403
848 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43150

Sequence Viewer

Length: 639 bp
ATGAGGAATTTTAGTGTGGTTTCTTTTCTGCTTCTCAAATTGGTAATGTTATGGTGCATAACCGCCAGCCGTTGTCCGGATTGTGGCCGCACCACCGTGCCGTATCCACTGAGCACGGAGCCAAACTGCGGTGACCAGGCAAACAAGGTTCAGTGCAATGCAGGAACACTGTGGTTCCACACCCTCAACAACTCCTATCCAGTCACATCCGTTAATCCGCTAACGCAGCGGATTGTGATCCAACCACCAGGAGTGGCAAACAAGAACACATGTGTCGCTGCTGATTTCCAATCTCAGGGAATTTTGCTGGACAACAACCTTCCCTTCAACATTAGTGGCAGCAACACTGTGATAGGCATGAACTGTTCAACTGAAATGCTTACCACCTCCTTAAACTGCTCCTCCAATAGCATATGCCACGACTACGTTAGGTCTTGTGGGAACTTGTCATTATGTTGTTCATACAAAACTGGTGGATCTTCAAATGCCTACAAAATCAGGGTTAGAAAGGAACGTTGTGTTGCCTATGTGAGTTTTGTGAATTTGGACCCTACGTTGGATGTGAGCAAGTGGCCTCAGCCTGCGGTTGAGATGCAATGGGTGTTGCCGGAAGAGCCAGGATGGGCAAGTTACAAGTAG

Protein Analysis

212

Amino Acids

23.36

Weight (kDa)

8.21

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 80 6.8e-07 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53840
malus_domestica MD12G1149600.v1.1
prunus_persica Prupe.6G261100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0465331 RchiOBHm_Chr3g0465341
rosa_laevigata RLG00000024646
rosa_multiflora Rmu_co8329097.1_g000001 Rmu_co8386781.1_g000001
rosa_roxburghii Rroxscaffold_6G00415120
rosa_rugosa Rorug03G0076200 Rorug03G0076300
rosa_samantha Rh3BG141100 Rh3CG142000 Rh3CG142100 Rh3DG142100 Rh3DG142200
rosa_wichuraiana Rw3G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 6 cut(s) 63, 88, 129, 218, 229, 584
AclI AACGTT 1 cut(s) 514
AclWI GGATC 2 cut(s) 232, 484
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 3 cut(s) 7, 300, 541
AfiI CCNNNNNNNGG 5 cut(s) 76, 83, 128, 295, 556
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 3 cut(s) 328, 369, 483
AjnI CCWGG 3 cut(s) 135, 247, 616
AleI CACNNNNGTG 1 cut(s) 95
Alw21I GWGCWC 1 cut(s) 116
AlwI GGATC 2 cut(s) 232, 484
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 2 cut(s) 85, 572
ApeKI GCWGC 3 cut(s) 226, 278, 339
ApoI RAATTY 3 cut(s) 7, 300, 541
AspS9I GGNCC 1 cut(s) 547
AsuHPI GGTGA 1 cut(s) 143
AvaII GGWCC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 116
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 3 cut(s) 238, 265, 351
BccI CCATC 1 cut(s) 615
BceAI ACGGC 2 cut(s) 54, 85
BciT130I CCWGG 3 cut(s) 137, 249, 618
BciVI GTATCC 1 cut(s) 114
BfuI GTATCC 1 cut(s) 114
BisI GCNGC 4 cut(s) 88, 227, 279, 340
BlsI GCNGC 4 cut(s) 89, 228, 280, 341
Bme1390I CCNGG 3 cut(s) 137, 249, 618
Bme18I GGWCC 1 cut(s) 547
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 3 cut(s) 120, 176, 549
BmrFI CCNGG 3 cut(s) 137, 249, 618
BmsI GCATC 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 83, 128, 295, 556
Bse1I ACTGG 2 cut(s) 200, 475
Bse3DI GCAATG 2 cut(s) 163, 602
Bse8I GATNNNNATC 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 76
BseBI CCWGG 3 cut(s) 137, 249, 618
BseGI GGATG 3 cut(s) 206, 565, 626
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 5 cut(s) 76, 83, 128, 295, 556
BseMI GCAATG 2 cut(s) 163, 602
BseMII CTCAG 3 cut(s) 101, 308, 590
BseNI ACTGG 2 cut(s) 200, 475
BseRI GAGGAG 1 cut(s) 391
BseXI GCAGC 3 cut(s) 238, 265, 351
BshFI GGCC 2 cut(s) 87, 574
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 2 cut(s) 77, 608
BslI CCNNNNNNNGG 5 cut(s) 76, 83, 128, 295, 556
BsnI GGCC 2 cut(s) 87, 574
Bsp1286I GDGCHC 1 cut(s) 116
Bsp13I TCCGGA 1 cut(s) 76
Bsp143I GATC 2 cut(s) 237, 476
BspACI CCGC 6 cut(s) 63, 88, 129, 218, 229, 584
BspANI GGCC 2 cut(s) 87, 574
BspCNI CTCAG 3 cut(s) 102, 307, 589
BspEI TCCGGA 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 120, 176, 549
BspPI GGATC 2 cut(s) 232, 484
BspQI GCTCTTC 1 cut(s) 606
BsrDI GCAATG 2 cut(s) 163, 602
BsrI ACTGG 2 cut(s) 200, 475
BssMI GATC 2 cut(s) 237, 476
Bst2UI CCWGG 3 cut(s) 137, 249, 618
Bst4CI ACNGT 4 cut(s) 97, 171, 349, 365
Bst6I CTCTTC 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 67, 582
BstDEI CTNAG 3 cut(s) 110, 294, 576
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 3 cut(s) 206, 565, 626
BstKTI GATC 2 cut(s) 240, 479
BstMBI GATC 2 cut(s) 237, 476
BstMWI GCNNNNNNNGC 2 cut(s) 226, 613
BstNI CCWGG 3 cut(s) 137, 249, 618
BstNSI RCATGY 1 cut(s) 273
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 3 cut(s) 135, 247, 616
BstV1I GCAGC 3 cut(s) 238, 265, 351
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BsuI GTATCC 1 cut(s) 114
BsuRI GGCC 2 cut(s) 87, 574
BtsCI GGATG 3 cut(s) 206, 565, 626
BtsIMutI CAGTG 4 cut(s) 107, 158, 167, 345
Cac8I GCNNGC 2 cut(s) 67, 582
Cfr13I GGNCC 1 cut(s) 547
CspCI CAANNNNNGTGG 4 cut(s) 316, 351, 454, 489
CviAII CATG 2 cut(s) 270, 358
CviJI RGCY 6 cut(s) 69, 87, 121, 574, 580, 616
CviKI_1 RGCY 6 cut(s) 69, 87, 121, 574, 580, 616
DdeI CTNAG 3 cut(s) 110, 294, 576
DpnI GATC 2 cut(s) 239, 478
DpnII GATC 2 cut(s) 237, 476
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco47I GGWCC 1 cut(s) 547
Eco91I GGTNACC 1 cut(s) 131
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 135, 247, 616
FaeI CATG 2 cut(s) 273, 361
FaiI YATR 9 cut(s) 52, 59, 271, 359, 413, 415, 454, 463, 528
FatI CATG 2 cut(s) 269, 357
FauNDI CATATG 1 cut(s) 413
Fnu4HI GCNGC 4 cut(s) 88, 227, 279, 340
FokI GGATG 3 cut(s) 193, 572, 633
Fsp4HI GCNGC 4 cut(s) 88, 227, 279, 340
GluI GCNGC 4 cut(s) 88, 227, 279, 340
HaeIII GGCC 2 cut(s) 87, 574
HapII CCGG 2 cut(s) 77, 608
Hin1II CATG 2 cut(s) 273, 361
HpaII CCGG 2 cut(s) 77, 608
HphI GGTGA 1 cut(s) 143
Hpy188III TCNNGA 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 329, 334
HpyCH4III ACNGT 4 cut(s) 97, 171, 349, 365
HpyCH4IV ACGT 3 cut(s) 426, 514, 554
HpyCH4V TGCA 4 cut(s) 57, 156, 161, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 613
HpyF3I CTNAG 3 cut(s) 110, 294, 576
HpySE526I ACGT 3 cut(s) 426, 514, 554
Hsp92II CATG 2 cut(s) 273, 361
Kpn2I TCCGGA 1 cut(s) 76
Kzo9I GATC 2 cut(s) 237, 476
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 2 cut(s) 118, 404
Lsp1109I GCAGC 3 cut(s) 238, 265, 351
LweI GCATC 1 cut(s) 582
MaeII ACGT 3 cut(s) 426, 514, 554
MaeIII GTNAC 3 cut(s) 131, 202, 629
MalI GATC 2 cut(s) 239, 478
MboI GATC 2 cut(s) 237, 476
MboII GAAGA 2 cut(s) 471, 623
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 4 cut(s) 7, 38, 300, 541
MmeI TCCRAC 2 cut(s) 265, 537
MnlI CCTC 4 cut(s) 194, 397, 412, 585
MroI TCCGGA 1 cut(s) 76
MseI TTAA 2 cut(s) 213, 392
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 2 cut(s) 77, 608
MspR9I CCNGG 3 cut(s) 137, 249, 618
MvaI CCWGG 3 cut(s) 137, 249, 618
MwoI GCNNNNNNNGC 2 cut(s) 226, 613
NdeI CATATG 1 cut(s) 413
NdeII GATC 2 cut(s) 237, 476
NlaIII CATG 2 cut(s) 273, 361
NlaIV GGNNCC 3 cut(s) 120, 176, 549
NmuCI GTSAC 2 cut(s) 131, 202
NspI RCATGY 1 cut(s) 273
OliI CACNNNNGTG 1 cut(s) 95
PciI ACATGT 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 606
PkrI GCNGC 4 cut(s) 89, 228, 280, 341
PscI ACATGT 1 cut(s) 269
Psp1406I AACGTT 1 cut(s) 514
Psp6I CCWGG 3 cut(s) 135, 247, 616
PspEI GGTNACC 1 cut(s) 131
PspGI CCWGG 3 cut(s) 135, 247, 616
PspN4I GGNNCC 3 cut(s) 120, 176, 549
PspPI GGNCC 1 cut(s) 547
PsuI RGATCY 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 2 cut(s) 213, 392
SatI GCNGC 4 cut(s) 88, 227, 279, 340
Sau3AI GATC 2 cut(s) 237, 476
Sau96I GGNCC 1 cut(s) 547
ScrFI CCNGG 3 cut(s) 137, 249, 618
SduI GDGCHC 1 cut(s) 116
SetI ASST 7 cut(s) 150, 321, 389, 429, 434, 517, 557
SfaNI GCATC 1 cut(s) 582
SinI GGWCC 1 cut(s) 547
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 4 cut(s) 7, 38, 300, 541
SsiI CCGC 6 cut(s) 63, 88, 129, 218, 229, 584
StyD4I CCNGG 3 cut(s) 135, 247, 616
TaaI ACNGT 4 cut(s) 97, 171, 349, 365
TaiI ACGT 3 cut(s) 429, 517, 557
TasI AATT 4 cut(s) 7, 38, 300, 541
TauI GCSGC 1 cut(s) 90
Tru1I TTAA 2 cut(s) 213, 392
Tru9I TTAA 2 cut(s) 213, 392
TscAI CASTG 4 cut(s) 114, 158, 174, 352
TseFI GTSAC 2 cut(s) 131, 202
TseI GCWGC 3 cut(s) 226, 278, 339
Tsp45I GTSAC 2 cut(s) 131, 202
TspDTI ATGAA 2 cut(s) 374, 450
TspGWI ACGGA 2 cut(s) 131, 199
TspRI CASTG 4 cut(s) 114, 158, 174, 352
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 3 cut(s) 7, 300, 541
XceI RCATGY 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.