Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0013898

Physical Location & Seq

Genomic Coordinates Forward (+)

37812064 .. 37814691

Gene Structure 2628 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024646

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGTTATGGTGCATAACCGCCAGCCGTTGTCCTGATTGTGGCCGCACCACCGTGCCGTATCCACTGAGCACGGAGCCAAACTGCGGCGACCAGGCATACAAGGTTCAGTGCAATGCAGGAACACTCTGGTTCCACACCGTCAACAACTCCTATCCAGTCACATCCGTTAATCCGCTAACGCAGCGGATTGTGATCCAACCACCAGGATTGGCAAACAAGAACACGTGTGTCGCTGCCGATTTCCAATCTCAGGGAATTTTGCTGGACAACAACCTTCCCTTCAACATTAGTGGCAGTAACACTGTGATAAGCATGAACTGTTCAACTGAAATGCTTACCACCTCCTTAAACTGCTCCTCCAATAGCATATGCCACGACTACGTTAGGCACAATCCAATGGCAGCATCAGCTTGTGGGAACTTGTCATTATGTTGTTCATACAAAACTGGTGGATCTTCAAATGCCTACAAAATCAGGGTTAGAAAGGAACGTTGTGTTGCCTATGTGAGTTTTGTGAATTTGGACTCTACGTTGGATGTGAGCAAGTGGCCTCAGCCTGCGGTTGAGATGCAATGGGTGTTGCCGGAAGAGCCAGGATGCAAGTTACAAGTAGATTGTGGGGGTTTGGCAAACTCGGTGTGCTTGCCGGATCCGTCTAGTGCCAAAGAGAGAAAGTGCTTGTGCAAGACTGGGTTCAAATGGGACCCCATCAATGGAATATGTCAAAGCTCCGCCTTTGCTGTTGTTGCAATGCTAATTGGAGGATCCTCTGCCTTTATAATTAAGCGACGTGGTCGAAACGTTGCAGGATCAGCACAGTTTAGCTTGACAAAGTTCAGAGAAGACATACTAAATGCTAACAGTAGTACTGGCAAATCAGCCAAGACTTTCACAGGCAAAGAAATCACAAAAGCAACCAACAACTTCTCCAAAGACAACCTTCTTGGCTCCGGTGGCTTTGGCGATGTCTTCAAGGCCACACTTGACGATGGAACTGTCATAGCAGTGAAGAAAGCCAAGGCTGGAAACATAAAGGGCATCGATCAAATTCTCAACGAAGTTCGAATTCTTTGCCAAGTAAACCACAGAAGCCTAGTCAGATTAATGGGTTGTTGTCTTGAGCTTGAACAGCCGCTTTTAATCTACGAGTATGTCCCAAACGGTACCCTTTTTGATCGTCTACATGGAAGTCACCACAGTGCCACCAAAAGGGTATCCCTAACGTGGGCCACGAGGCTCACCATTGCTCACCAAACTGCTGAAGGACTTGCCTATCTGCACGATTCAGTCGAGCCAAGGATTTACCACCGTGACATAAAGTCGAGCAACATTTTACTCGATGAAAAGCTCAATGCTAAGGTTGCTGATTTTGGTCTGTCGAGGTTGGCCGTGACCGAATCAAGTCACATTACAACATGTGCTCAGGGAACCTTGGGGTACCTAGACCCCGACTATTACTTGAACTTTCAATTGACAGACAAGAGTGATGTCTATAGCTTTGGAGTTGTGTTGTTAGAGCTGTTGACTGGTAAGAAAGCTATTGATTTTAACAGACAAGAAGACGACGTGAACTTGGTGGTTTATGTCAAGAACATTTTAAGGGAAGAGAGACTAAATGATGCCATTGACCCTAAGCTTAAAGAGGAGGCTGGCACAATGGCTCTGGAGACTATGAAGGCATTAGGGTCTCTGGCAGCTGCATGCTTGGATGAGAGGAGGCAAAATCGACCCTCCATGAAAGAAGTCGCTGATGAAATTGAGTACATTATTAGTATCGTTGCAAATGAGGTTTCGGCGACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

601

Amino Acids

65.68

Weight (kDa)

7.41

Isoelectric Point (pI)

34.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	10 - 65	1.4e-07	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	310 - 583	1e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	312 - 585	6e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53840
malus_domestica	MD12G1149600.v1.1
prunus_persica	Prupe.6G261100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0465331 RchiOBHm_Chr3g0465341
rosa_laevigata	RLG00000024646
rosa_multiflora	Rmu_co8329097.1_g000001 Rmu_co8386781.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415120
rosa_rugosa	Rorug03G0076200 Rorug03G0076300
rosa_samantha	Rh3BG141100 Rh3CG142000 Rh3CG142100 Rh3DG142100 Rh3DG142200
rosa_wichuraiana	Rw3G011540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	779
Acc65I	GGTACC	2 cut(s)	1163, 1437
AccB1I	GGYRCC	2 cut(s)	1163, 1437
AccI	GTMKAC	1 cut(s)	1180
AciI	CCGC	8 cut(s)	18, 43, 84, 173, 184, 560, 732, 1133
AclI	AACGTT	2 cut(s)	490, 801
AclWI	GGATC	7 cut(s)	187, 460, 644, 657, 759, 772, 817
AcoI	YGGCCR	2 cut(s)	40, 1386
AcsI	RAATTY	4 cut(s)	255, 517, 1047, 1065
AcuI	CTGAAG	1 cut(s)	1281
AcvI	CACGTG	1 cut(s)	225
AfaI	GTAC	4 cut(s)	868, 1165, 1439, 1763
AfiI	CCNNNNNNNGG	7 cut(s)	38, 83, 250, 713, 1209, 1224, 1225
AflIII	ACRYGT	3 cut(s)	222, 224, 1415
AgsI	TTSAA	8 cut(s)	283, 324, 459, 697, 973, 1127, 1462, 1469
AhdI	GACNNNNNGTC	1 cut(s)	1318
AjiI	CACGTC	2 cut(s)	791, 1567
AjnI	CCWGG	3 cut(s)	90, 202, 592
AleI	CACNNNNGTG	2 cut(s)	50, 1197
Alw21I	GWGCWC	2 cut(s)	71, 1423
Alw26I	GTCTC	3 cut(s)	1603, 1661, 1692
AlwI	GGATC	7 cut(s)	187, 460, 644, 657, 759, 772, 817
AoxI	GGCC	5 cut(s)	40, 548, 975, 1227, 1386
ApeKI	GCWGC	5 cut(s)	181, 233, 401, 1694, 1697
ApoI	RAATTY	4 cut(s)	255, 517, 1047, 1065
AseI	ATTAAT	1 cut(s)	1103
Asp718I	GGTACC	2 cut(s)	1163, 1437
AspS9I	GGNCC	2 cut(s)	703, 1227
AsuHPI	GGTGA	3 cut(s)	1184, 1231, 1241
AsuII	TTCGAA	1 cut(s)	1063
AvaII	GGWCC	1 cut(s)	703
BamHI	GGATCC	2 cut(s)	649, 764
BanI	GGYRCC	2 cut(s)	1163, 1437
BauI	CACGAG	1 cut(s)	1231
BbrPI	CACGTG	1 cut(s)	225
BbsI	GAAGAC	3 cut(s)	849, 961, 1566
Bbv12I	GWGCWC	2 cut(s)	71, 1423
BbvCI	CCTCAGC	1 cut(s)	552
BbvI	GCAGC	5 cut(s)	193, 220, 413, 1684, 1706
BccI	CCATC	2 cut(s)	716, 983
BceAI	ACGGC	3 cut(s)	9, 40, 1373
BcgI	CGANNNNNNTGC	2 cut(s)	1053, 1087
BciT130I	CCWGG	3 cut(s)	92, 204, 594
BciVI	GTATCC	2 cut(s)	69, 1225
BcoDI	GTCTC	3 cut(s)	1603, 1661, 1692
BfaI	CTAG	3 cut(s)	657, 1094, 1442
BfmI	CTRYAG	1 cut(s)	1492
BfuI	GTATCC	2 cut(s)	69, 1225
BisI	GCNGC	8 cut(s)	43, 85, 182, 234, 402, 1133, 1695, 1698
BlsI	GCNGC	8 cut(s)	44, 86, 183, 235, 403, 1134, 1696, 1699
BmcAI	AGTACT	1 cut(s)	868
Bme1390I	CCNGG	3 cut(s)	92, 204, 594
Bme18I	GGWCC	1 cut(s)	703
BmeRI	GACNNNNNGTC	1 cut(s)	1318
BmgBI	CACGTC	2 cut(s)	791, 1567
BmgT120I	GGNCC	2 cut(s)	703, 1227
BmrFI	CCNGG	3 cut(s)	92, 204, 594
BmrI	ACTGGG	1 cut(s)	699
BmsI	GCATC	5 cut(s)	413, 558, 587, 1047, 1609
BmuI	ACTGGG	1 cut(s)	699
BpiI	GAAGAC	3 cut(s)	849, 961, 1566
BpmI	CTGGAG	1 cut(s)	1685
Bpu10I	CCTNAGC	4 cut(s)	552, 1356, 1422, 1632
Bpu14I	TTCGAA	1 cut(s)	1063
BpuEI	CTTGAG	1 cut(s)	1139
Bsa29I	ATCGAT	1 cut(s)	1041
BsaAI	YACGTR	1 cut(s)	225
BsaBI	GATNNNNATC	1 cut(s)	191
BsaI	GGTCTC	1 cut(s)	1692
BsaJI	CCNNGG	3 cut(s)	1017, 1295, 1431
BsaWI	WCCGGW	1 cut(s)	950
BsaXI	ACNNNNNCTCC	4 cut(s)	341, 371, 911, 941
Bsc4I	CCNNNNNNNGG	7 cut(s)	38, 83, 250, 713, 1209, 1224, 1225
Bse1I	ACTGG	5 cut(s)	155, 451, 694, 874, 1531
Bse3DI	GCAATG	4 cut(s)	118, 578, 756, 1242
Bse8I	GATNNNNATC	1 cut(s)	191
BseBI	CCWGG	3 cut(s)	92, 204, 594
BseCI	ATCGAT	1 cut(s)	1041
BseDI	CCNNGG	3 cut(s)	1017, 1295, 1431
BseGI	GGATG	4 cut(s)	161, 541, 602, 1714
BseJI	GATNNNNATC	1 cut(s)	191
BseLI	CCNNNNNNNGG	7 cut(s)	38, 83, 250, 713, 1209, 1224, 1225
BseMI	GCAATG	4 cut(s)	118, 578, 756, 1242
BseMII	CTCAG	4 cut(s)	56, 263, 566, 1436
BseNI	ACTGG	5 cut(s)	155, 451, 694, 874, 1531
BseRI	GAGGAG	3 cut(s)	346, 1658, 1729
BseXI	GCAGC	5 cut(s)	193, 220, 413, 1684, 1706
BsgI	GTGCAG	1 cut(s)	1262
BshFI	GGCC	5 cut(s)	42, 550, 977, 1229, 1388
BshNI	GGYRCC	2 cut(s)	1163, 1437
BshVI	ATCGAT	1 cut(s)	1041
BsiHKAI	GWGCWC	2 cut(s)	71, 1423
BsiSI	CCGG	3 cut(s)	584, 647, 951
BslFI	GGGAC	2 cut(s)	716, 1139
BslI	CCNNNNNNNGG	7 cut(s)	38, 83, 250, 713, 1209, 1224, 1225
BsmAI	GTCTC	3 cut(s)	1603, 1661, 1692
BsmFI	GGGAC	2 cut(s)	716, 1139
BsnI	GGCC	5 cut(s)	42, 550, 977, 1229, 1388
Bso31I	GGTCTC	1 cut(s)	1692
Bsp119I	TTCGAA	1 cut(s)	1063
Bsp1286I	GDGCHC	2 cut(s)	71, 1423
Bsp143I	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
BspACI	CCGC	8 cut(s)	18, 43, 84, 173, 184, 560, 732, 1133
BspANI	GGCC	5 cut(s)	42, 550, 977, 1229, 1388
BspCNI	CTCAG	4 cut(s)	57, 262, 565, 1435
BspDI	ATCGAT	1 cut(s)	1041
BspPI	GGATC	7 cut(s)	187, 460, 644, 657, 759, 772, 817
BspQI	GCTCTTC	1 cut(s)	582
BspT104I	TTCGAA	1 cut(s)	1063
BspT107I	GGYRCC	2 cut(s)	1163, 1437
BspTNI	GGTCTC	1 cut(s)	1692
BsrDI	GCAATG	4 cut(s)	118, 578, 756, 1242
BsrI	ACTGG	5 cut(s)	155, 451, 694, 874, 1531
BssECI	CCNNGG	3 cut(s)	1017, 1295, 1431
BssMI	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
BssSI	CACGAG	1 cut(s)	1231
BssT1I	CCWWGG	3 cut(s)	1017, 1295, 1431
Bst2BI	CACGAG	1 cut(s)	1231
Bst2UI	CCWGG	3 cut(s)	92, 204, 594
Bst6I	CTCTTC	2 cut(s)	582, 1599
BstBAI	YACGTR	1 cut(s)	225
BstBI	TTCGAA	1 cut(s)	1063
BstC8I	GCNNGC	5 cut(s)	22, 558, 644, 1651, 1702
BstDEI	CTNAG	7 cut(s)	65, 249, 552, 1356, 1422, 1632, 1800
BstF5I	GGATG	4 cut(s)	161, 541, 602, 1714
BstKTI	GATC	7 cut(s)	195, 455, 652, 767, 812, 1045, 1177
BstMAI	GTCTC	3 cut(s)	1603, 1661, 1692
BstMBI	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
BstMWI	GCNNNNNNNGC	8 cut(s)	181, 407, 589, 746, 812, 954, 1129, 1361
BstNI	CCWGG	3 cut(s)	92, 204, 594
BstNSI	RCATGY	2 cut(s)	1419, 1704
BstSCI	CCNGG	3 cut(s)	90, 202, 592
BstSFI	CTRYAG	1 cut(s)	1492
BstV1I	GCAGC	5 cut(s)	193, 220, 413, 1684, 1706
BstV2I	GAAGAC	3 cut(s)	849, 961, 1566
BstX2I	RGATCY	3 cut(s)	452, 649, 764
BstYI	RGATCY	3 cut(s)	452, 649, 764
Bsu15I	ATCGAT	1 cut(s)	1041
BsuI	GTATCC	2 cut(s)	69, 1225
BsuRI	GGCC	5 cut(s)	42, 550, 977, 1229, 1388
BsuTUI	ATCGAT	1 cut(s)	1041
BtgZI	GCGATG	1 cut(s)	978
BtrI	CACGTC	2 cut(s)	791, 1567
BtsCI	GGATG	4 cut(s)	161, 541, 602, 1714
BtsI	GCAGTG	1 cut(s)	1011
BtsIMutI	CAGTG	5 cut(s)	62, 113, 300, 1011, 1204
Cac8I	GCNNGC	5 cut(s)	22, 558, 644, 1651, 1702
Cfr13I	GGNCC	2 cut(s)	703, 1227
ClaI	ATCGAT	1 cut(s)	1041
Csp6I	GTAC	4 cut(s)	867, 1164, 1438, 1762
CspCI	CAANNNNNGTGG	6 cut(s)	189, 224, 271, 306, 430, 465
CviAII	CATG	5 cut(s)	313, 1184, 1416, 1701, 1735
CviQI	GTAC	4 cut(s)	867, 1164, 1438, 1762
DdeI	CTNAG	7 cut(s)	65, 249, 552, 1356, 1422, 1632, 1800
DpnI	GATC	7 cut(s)	194, 454, 651, 766, 811, 1044, 1176
DpnII	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
DriI	GACNNNNNGTC	1 cut(s)	1318
EaeI	YGGCCR	2 cut(s)	40, 1386
Eam1104I	CTCTTC	2 cut(s)	582, 1599
Eam1105I	GACNNNNNGTC	1 cut(s)	1318
EarI	CTCTTC	2 cut(s)	582, 1599
EciI	GGCGGA	1 cut(s)	721
Eco130I	CCWWGG	3 cut(s)	1017, 1295, 1431
Eco31I	GGTCTC	1 cut(s)	1692
Eco47I	GGWCC	1 cut(s)	703
Eco57I	CTGAAG	1 cut(s)	1281
Eco72I	CACGTG	1 cut(s)	225
EcoO109I	RGGNCCY	1 cut(s)	703
EcoRI	GAATTC	1 cut(s)	1065
EcoRII	CCWGG	3 cut(s)	90, 202, 592
EcoT14I	CCWWGG	3 cut(s)	1017, 1295, 1431
ErhI	CCWWGG	3 cut(s)	1017, 1295, 1431
FaeI	CATG	5 cut(s)	316, 1187, 1419, 1704, 1738
FalI	AAGNNNNNCTT	2 cut(s)	924, 956
FaqI	GGGAC	2 cut(s)	716, 1139
FatI	CATG	5 cut(s)	312, 1183, 1415, 1700, 1734
FauNDI	CATATG	1 cut(s)	368
FblI	GTMKAC	1 cut(s)	1180
Fnu4HI	GCNGC	8 cut(s)	43, 85, 182, 234, 402, 1133, 1695, 1698
FokI	GGATG	4 cut(s)	148, 548, 609, 1721
Fsp4HI	GCNGC	8 cut(s)	43, 85, 182, 234, 402, 1133, 1695, 1698
FspBI	CTAG	3 cut(s)	657, 1094, 1442
GluI	GCNGC	8 cut(s)	43, 85, 182, 234, 402, 1133, 1695, 1698
GsuI	CTGGAG	1 cut(s)	1685
HaeIII	GGCC	5 cut(s)	42, 550, 977, 1229, 1388
HapII	CCGG	3 cut(s)	584, 647, 951
Hin1II	CATG	5 cut(s)	316, 1187, 1419, 1704, 1738
HincII	GTYRAC	2 cut(s)	142, 1524
HindII	GTYRAC	2 cut(s)	142, 1524
HindIII	AAGCTT	1 cut(s)	1634
HinfI	GANTC	3 cut(s)	524, 1283, 1397
HpaII	CCGG	3 cut(s)	584, 647, 951
HphI	GGTGA	3 cut(s)	1184, 1231, 1241
Hpy166II	GTNNAC	5 cut(s)	142, 1081, 1181, 1524, 1570
Hpy188I	TCNGA	2 cut(s)	839, 1100
Hpy188III	TCNNGA	4 cut(s)	32, 1118, 1588, 1664
Hpy8I	GTNNAC	5 cut(s)	142, 1081, 1181, 1524, 1570
Hpy99I	CGWCG	2 cut(s)	792, 1568
HpyAV	CCTTC	5 cut(s)	284, 289, 950, 1256, 1669
HpyCH4IV	ACGT	8 cut(s)	224, 381, 490, 530, 790, 801, 1223, 1566
HpyF10VI	GCNNNNNNNGC	8 cut(s)	181, 407, 589, 746, 812, 954, 1129, 1361
HpyF3I	CTNAG	7 cut(s)	65, 249, 552, 1356, 1422, 1632, 1800
HpySE526I	ACGT	8 cut(s)	224, 381, 490, 530, 790, 801, 1223, 1566
Hsp92II	CATG	5 cut(s)	316, 1187, 1419, 1704, 1738
KflI	GGGWCCC	1 cut(s)	703
KpnI	GGTACC	2 cut(s)	1167, 1441
Kzo9I	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
LguI	GCTCTTC	1 cut(s)	582
LmnI	GCTCC	4 cut(s)	73, 359, 734, 953
Lsp1109I	GCAGC	5 cut(s)	193, 220, 413, 1684, 1706
LweI	GCATC	5 cut(s)	413, 558, 587, 1047, 1609
MaeI	CTAG	3 cut(s)	657, 1094, 1442
MaeII	ACGT	8 cut(s)	224, 381, 490, 530, 790, 801, 1223, 1566
MaeIII	GTNAC	7 cut(s)	157, 296, 603, 1190, 1310, 1390, 1403
MalI	GATC	7 cut(s)	194, 454, 651, 766, 811, 1044, 1176
MboI	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
MboII	GAAGA	7 cut(s)	447, 599, 854, 961, 1021, 1571, 1616
MfeI	CAATTG	1 cut(s)	1469
MflI	RGATCY	3 cut(s)	452, 649, 764
MhlI	GDGCHC	2 cut(s)	71, 1423
MluCI	AATT	8 cut(s)	255, 517, 756, 780, 1047, 1065, 1469, 1755
MlyI	GAGTC	1 cut(s)	518
MmeI	TCCRAC	2 cut(s)	220, 513
MseI	TTAA	8 cut(s)	168, 347, 783, 1103, 1139, 1548, 1598, 1638
MslI	CAYNNNNRTG	3 cut(s)	50, 1004, 1197
MspA1I	CMGCKG	2 cut(s)	184, 1697
MspI	CCGG	3 cut(s)	584, 647, 951
MspR9I	CCNGG	3 cut(s)	92, 204, 594
MunI	CAATTG	1 cut(s)	1469
MvaI	CCWGG	3 cut(s)	92, 204, 594
MwoI	GCNNNNNNNGC	8 cut(s)	181, 407, 589, 746, 812, 954, 1129, 1361
NdeI	CATATG	1 cut(s)	368
NdeII	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
NlaIII	CATG	5 cut(s)	316, 1187, 1419, 1704, 1738
NmuCI	GTSAC	5 cut(s)	157, 1190, 1310, 1390, 1403
NspI	RCATGY	2 cut(s)	1419, 1704
NspV	TTCGAA	1 cut(s)	1063
OliI	CACNNNNGTG	2 cut(s)	50, 1197
PaeI	GCATGC	1 cut(s)	1704
PciI	ACATGT	1 cut(s)	1415
PciSI	GCTCTTC	1 cut(s)	582
PcsI	WCGNNNNNNNCGW	2 cut(s)	1229, 1287
PfeI	GAWTC	2 cut(s)	1283, 1397
PflFI	GACNNNGTC	1 cut(s)	792
PkrI	GCNGC	8 cut(s)	44, 86, 183, 235, 403, 1134, 1696, 1699
PleI	GAGTC	1 cut(s)	518
PmaCI	CACGTG	1 cut(s)	225
PmlI	CACGTG	1 cut(s)	225
PpsI	GAGTC	1 cut(s)	518
Ppu21I	YACGTR	1 cut(s)	225
PpuMI	RGGWCCY	1 cut(s)	703
PscI	ACATGT	1 cut(s)	1415
PshBI	ATTAAT	1 cut(s)	1103
PsiI	TTATAA	1 cut(s)	779
Psp1406I	AACGTT	2 cut(s)	490, 801
Psp5II	RGGWCCY	1 cut(s)	703
Psp6I	CCWGG	3 cut(s)	90, 202, 592
PspCI	CACGTG	1 cut(s)	225
PspGI	CCWGG	3 cut(s)	90, 202, 592
PspPI	GGNCC	2 cut(s)	703, 1227
PspPPI	RGGWCCY	1 cut(s)	703
PsuI	RGATCY	3 cut(s)	452, 649, 764
PsyI	GACNNNGTC	1 cut(s)	792
PvuII	CAGCTG	1 cut(s)	1697
RsaI	GTAC	4 cut(s)	868, 1165, 1439, 1763
RsaNI	GTAC	4 cut(s)	867, 1164, 1438, 1762
RseI	CAYNNNNRTG	3 cut(s)	50, 1004, 1197
SapI	GCTCTTC	1 cut(s)	582
SaqAI	TTAA	8 cut(s)	168, 347, 783, 1103, 1139, 1548, 1598, 1638
SatI	GCNGC	8 cut(s)	43, 85, 182, 234, 402, 1133, 1695, 1698
Sau3AI	GATC	7 cut(s)	192, 452, 649, 764, 809, 1042, 1174
Sau96I	GGNCC	2 cut(s)	703, 1227
ScaI	AGTACT	1 cut(s)	868
SchI	GAGTC	1 cut(s)	518
ScrFI	CCNGG	3 cut(s)	92, 204, 594
SduI	GDGCHC	2 cut(s)	71, 1423
SfaNI	GCATC	5 cut(s)	413, 558, 587, 1047, 1609
SfcI	CTRYAG	1 cut(s)	1492
SfuI	TTCGAA	1 cut(s)	1063
SinI	GGWCC	1 cut(s)	703
SmiMI	CAYNNNNRTG	3 cut(s)	50, 1004, 1197
SmlI	CTYRAG	1 cut(s)	1118
SmoI	CTYRAG	1 cut(s)	1118
SphI	GCATGC	1 cut(s)	1704
Sse9I	AATT	8 cut(s)	255, 517, 756, 780, 1047, 1065, 1469, 1755
SsiI	CCGC	8 cut(s)	18, 43, 84, 173, 184, 560, 732, 1133
SspMI	CTAG	3 cut(s)	657, 1094, 1442
StyD4I	CCNGG	3 cut(s)	90, 202, 592
StyI	CCWWGG	3 cut(s)	1017, 1295, 1431
TaiI	ACGT	8 cut(s)	227, 384, 493, 533, 793, 804, 1226, 1569
TaqI	TCGA	8 cut(s)	796, 1041, 1063, 1290, 1322, 1338, 1379, 1726
TaqII	GACCGA	1 cut(s)	1409
TasI	AATT	8 cut(s)	255, 517, 756, 780, 1047, 1065, 1469, 1755
TatI	WGTACW	2 cut(s)	866, 1761
TauI	GCSGC	3 cut(s)	45, 87, 1135
TfiI	GAWTC	2 cut(s)	1283, 1397
Tru1I	TTAA	8 cut(s)	168, 347, 783, 1103, 1139, 1548, 1598, 1638
Tru9I	TTAA	8 cut(s)	168, 347, 783, 1103, 1139, 1548, 1598, 1638
TscAI	CASTG	5 cut(s)	69, 113, 307, 1011, 1204
TseFI	GTSAC	5 cut(s)	157, 1190, 1310, 1390, 1403
TseI	GCWGC	5 cut(s)	181, 233, 401, 1694, 1697
Tsp45I	GTSAC	5 cut(s)	157, 1190, 1310, 1390, 1403
TspDTI	ATGAA	6 cut(s)	329, 426, 1356, 1688, 1751, 1767
TspGWI	ACGGA	3 cut(s)	86, 154, 642
TspRI	CASTG	5 cut(s)	69, 113, 307, 1011, 1204
Tth111I	GACNNNGTC	1 cut(s)	792
VpaK11BI	GGWCC	1 cut(s)	703
VspI	ATTAAT	1 cut(s)	1103
XapI	RAATTY	4 cut(s)	255, 517, 1047, 1065
XceI	RCATGY	2 cut(s)	1419, 1704
XmiI	GTMKAC	1 cut(s)	1180
XspI	CTAG	3 cut(s)	657, 1094, 1442
ZrmI	AGTACT	1 cut(s)	868

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.