Rh3CG142100

Wall-associated receptor kinase-like 20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0013898
Physical Location & Seq
Reverse (-)
11794487 .. 11795044
558 bp
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UTR
Exon/CDS
Intron
Rh3CG142100.1

Sequence Viewer

Length: 558 bp
ATGAGGAATTTTAGTGTGGTTTCTTTTCTGCTTCTCAAATTGGTAATGTTATGGTGCATAACCGCCAGCCGTTGTCCGGATTGTGGCCGCACCACCGTGCCGTATCCACTGAGCACGGAGCCAAACTGCGGTGACCAGGCAAACAAGGTTCAGTGCAATGCAGGAACACTGTGGTTCCACACCCTCAACAACTCCTATCCAGTCACATCCGTTAATCCGCTAACGCAGCGGATTGTGATCCAACCACCAGGAGTGGCAAACAAGAACACATGTGTCGCTGCTGATTTCCAATCTCAGGGAATTTTGCTGGACAACAACCTTCCCTTCAACATTAGTGGCAGCAACACTGTGATAGGCATGAACTGTTCAACTGAAATGCTTACCACCTCCTTAAACTGCTCCTCCAATAGCATATGCCACGACTACGTTAGGTGTTGTGATATTTCAATACTTCACCATAAATTAGCAGTTGCTTCAGTATTAATATCAACTCTTCTATCAGAAAAGAAAAACATTTCTACATTACAAGCACTCCTAAACAAATCCGTAATCTATTAG

Protein Analysis

185

Amino Acids

20.2

Weight (kDa)

8.31

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 80 5.2e-07 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53840
malus_domestica MD12G1149600.v1.1
prunus_persica Prupe.6G261100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0465331 RchiOBHm_Chr3g0465341
rosa_laevigata RLG00000024646
rosa_multiflora Rmu_co8329097.1_g000001 Rmu_co8386781.1_g000001
rosa_roxburghii Rroxscaffold_6G00415120
rosa_rugosa Rorug03G0076200 Rorug03G0076300
rosa_samantha Rh3BG141100 Rh3CG142000 Rh3CG142100 Rh3DG142100 Rh3DG142200
rosa_wichuraiana Rw3G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 5 cut(s) 63, 88, 129, 218, 229
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 2 cut(s) 7, 300
AcuI CTGAAG 1 cut(s) 459
AfiI CCNNNNNNNGG 4 cut(s) 76, 83, 128, 295
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 3 cut(s) 328, 369, 447
AjnI CCWGG 2 cut(s) 135, 247
AleI CACNNNNGTG 1 cut(s) 95
Alw21I GWGCWC 1 cut(s) 116
AlwI GGATC 1 cut(s) 232
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 3 cut(s) 226, 278, 339
ApoI RAATTY 2 cut(s) 7, 300
AseI ATTAAT 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 143, 446
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 238, 265, 351
BceAI ACGGC 2 cut(s) 54, 85
BciT130I CCWGG 2 cut(s) 137, 249
BciVI GTATCC 1 cut(s) 114
BfuI GTATCC 1 cut(s) 114
BisI GCNGC 4 cut(s) 88, 227, 279, 340
BlsI GCNGC 4 cut(s) 89, 228, 280, 341
Bme1390I CCNGG 2 cut(s) 137, 249
BmiI GGNNCC 2 cut(s) 120, 176
BmrFI CCNGG 2 cut(s) 137, 249
BsaBI GATNNNNATC 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 516, 546
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 83, 128, 295
Bse1I ACTGG 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 76
BseBI CCWGG 2 cut(s) 137, 249
BseGI GGATG 1 cut(s) 206
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 4 cut(s) 76, 83, 128, 295
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 101, 308
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 391
BseXI GCAGC 3 cut(s) 238, 265, 351
BshFI GGCC 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 4 cut(s) 76, 83, 128, 295
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 116
Bsp13I TCCGGA 1 cut(s) 76
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 5 cut(s) 63, 88, 129, 218, 229
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 102, 307
BspEI TCCGGA 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 120, 176
BspPI GGATC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 1 cut(s) 200
BssMI GATC 1 cut(s) 237
Bst2UI CCWGG 2 cut(s) 137, 249
Bst4CI ACNGT 4 cut(s) 97, 171, 349, 365
Bst6I CTCTTC 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 110, 294
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 1 cut(s) 206
BstKTI GATC 1 cut(s) 240
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 137, 249
BstNSI RCATGY 1 cut(s) 273
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 135, 247
BstV1I GCAGC 3 cut(s) 238, 265, 351
BsuI GTATCC 1 cut(s) 114
BsuRI GGCC 1 cut(s) 87
BtsCI GGATG 1 cut(s) 206
BtsIMutI CAGTG 4 cut(s) 107, 158, 167, 345
Cac8I GCNNGC 1 cut(s) 67
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviAII CATG 2 cut(s) 270, 358
CviJI RGCY 3 cut(s) 69, 87, 121
CviKI_1 RGCY 3 cut(s) 69, 87, 121
DdeI CTNAG 2 cut(s) 110, 294
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 459
Eco91I GGTNACC 1 cut(s) 131
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 135, 247
FaeI CATG 2 cut(s) 273, 361
FaiI YATR 7 cut(s) 52, 59, 271, 359, 413, 415, 459
FatI CATG 2 cut(s) 269, 357
FauNDI CATATG 1 cut(s) 413
Fnu4HI GCNGC 4 cut(s) 88, 227, 279, 340
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 88, 227, 279, 340
GluI GCNGC 4 cut(s) 88, 227, 279, 340
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 273, 361
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 143, 446
Hpy188I TCNGA 1 cut(s) 502
Hpy188III TCNNGA 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 329, 334
HpyCH4III ACNGT 4 cut(s) 97, 171, 349, 365
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 3 cut(s) 57, 156, 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 110, 294
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 2 cut(s) 273, 361
Kpn2I TCCGGA 1 cut(s) 76
Kzo9I GATC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 118, 404
Lsp1109I GCAGC 3 cut(s) 238, 265, 351
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 131, 202
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 4 cut(s) 7, 38, 300, 461
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 3 cut(s) 194, 397, 412
MroI TCCGGA 1 cut(s) 76
MseI TTAA 3 cut(s) 213, 392, 482
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 137, 249
MvaI CCWGG 2 cut(s) 137, 249
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeI CATATG 1 cut(s) 413
NdeII GATC 1 cut(s) 237
NlaIII CATG 2 cut(s) 273, 361
NlaIV GGNNCC 2 cut(s) 120, 176
NmuCI GTSAC 2 cut(s) 131, 202
NspI RCATGY 1 cut(s) 273
OliI CACNNNNGTG 1 cut(s) 95
PciI ACATGT 1 cut(s) 269
PkrI GCNGC 4 cut(s) 89, 228, 280, 341
PscI ACATGT 1 cut(s) 269
PshBI ATTAAT 1 cut(s) 482
Psp6I CCWGG 2 cut(s) 135, 247
PspEI GGTNACC 1 cut(s) 131
PspGI CCWGG 2 cut(s) 135, 247
PspN4I GGNNCC 2 cut(s) 120, 176
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 3 cut(s) 213, 392, 482
SatI GCNGC 4 cut(s) 88, 227, 279, 340
Sau3AI GATC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 137, 249
SduI GDGCHC 1 cut(s) 116
SetI ASST 5 cut(s) 150, 321, 389, 429, 434
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 4 cut(s) 7, 38, 300, 461
SsiI CCGC 5 cut(s) 63, 88, 129, 218, 229
StyD4I CCNGG 2 cut(s) 135, 247
TaaI ACNGT 4 cut(s) 97, 171, 349, 365
TaiI ACGT 1 cut(s) 429
TasI AATT 4 cut(s) 7, 38, 300, 461
TauI GCSGC 1 cut(s) 90
Tru1I TTAA 3 cut(s) 213, 392, 482
Tru9I TTAA 3 cut(s) 213, 392, 482
TscAI CASTG 4 cut(s) 114, 158, 174, 352
TseFI GTSAC 2 cut(s) 131, 202
TseI GCWGC 3 cut(s) 226, 278, 339
Tsp45I GTSAC 2 cut(s) 131, 202
TspDTI ATGAA 1 cut(s) 374
TspGWI ACGGA 3 cut(s) 131, 199, 535
TspRI CASTG 4 cut(s) 114, 158, 174, 352
VspI ATTAAT 1 cut(s) 482
XapI RAATTY 2 cut(s) 7, 300
XceI RCATGY 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.