Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006483

Physical Location & Seq

Genomic Coordinates Forward (+)

23164513 .. 23169859

Gene Structure 5347 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1151800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGCCTCCTACCAAATCCTCACCGAAACTCCACCATGAAGCCACAGTGATCAGATCAGCAATCCAGTCCAGACTCCTGGTCCTGACTCTAATCCTCCTATGGCGGACTCTCTTGTCCTCGTACGACACCTCCGCCCCCATCAATCCCGATTGCCTATCTACCATCCCTGCACAAAAAAACGTTCTCTTTCATTCCCTGGGTTTGGCTATCGAGTCCAGCATTGTATGGGACAGTGTCTACTATGTCCGGATTGCAGAGTGTGGCTACGAGTACGAGCAGACCTATGCTTTCTTCCCCCTTCTTCCTCTTTGCATGTCGTTCTTATCCCGCACAGTTTTGGCGCCATTGGTTCCAGTTATTGGGCAAAGAGCTGTGTTGGGATTATCCGGCTATGTGATTAATAACATTGGCTTCGTGTTTGGGGCAGTTTATTTATACAGGCTTTCAGTTGTCATTTTGAAGGACCAAGAAGCAGCAGTGAGGGCTTCGCTTTTGTTTTGCTTCAATCCAGCTTCCATATTCTATTCATCAATATATTCAGAGACATTGTTCGCCCTTTTTTCAGTTGGTGGATTGTACCATCTAATATCTGGAAAAGATGTCATTGCTGTTCTTTGGTTTGCTCTTTCGGGATTTTCAAGGTCCAATGGAGTGCTTAATGCTGGTTATTTCTGTTTTCAGACTATGCATCAGGCCTATGATGCTGTTTTCTTGAGAAAGCGCCCTTTTTTGGCATTGCAGGCTGTTGTTGGTGGAGCTCTGCGCTGTATATGTATATTTGTTCCTTTTATTGCATTTCAAGCATATGGATACAACAATCTCTGTCTTGGACATCTTCCATCCGATATGAGGCCCTGGTGCAATGCAAGGGTACCTTTGTTGTACAATTATATTCAAAGTCACTATTGGGGAGTAGGTTTCTTGAGATACTTCCAAGTTAAACAGTTGCCAAACTTTCTGCTGGCGTCACCAATATTGTCTCTGGCACTTTGCTCAATCATTCATTACGCGAAGTCAAAGCCTGAAAATTTCTTCTCTTTGGGTTTCCGTGCTCCTCCTGAGGACAAAGATTCTGCTGCTGTGCTCTTTTCTCTAACAGAATACTCCTCCAGAAAAAATCGCACAAGGAAGCAAGCGAACAAAGATGGTCCTGCTTTACTCTCAGAAGAACATAAAGCATTAGCAAAGCAAGGATACTTGTCTGTTGCTGTGCTTCCATGTATTCTACATTTGGGATTTATGGCAGCCACCGCATTTTTTGTCATGCATGTGCAGGTTGCTACTCGTTTCTTATCCTTCAGCCCTCCCCTCTATTGGTTTGCGTCGTATATAATGAAATCTCGTGGTACTGGTAAGCGATGGGGATATATAGTTTGGGTATACTCTGCTGCGTACATTCTTCTAGGCAGTTTGCTCTTTCCGAACTTCTATCCTTTCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000030 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016740 GO:0016757 GO:0016758 GO:0019538 GO:0019637 GO:0031501 GO:0031984 GO:0032991 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0070085 GO:0071704 GO:0090407 GO:0097502 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903509 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Mannosyl_trans2

Protein Analysis

481

Amino Acids

53.87

Weight (kDa)

9.35

Isoelectric Point (pI)

51.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mannosyl_trans2	PF04188	16 - 480	6.2e-148	Mannosyltransferase (PIG-V)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11880 AT1G11880 AT1G11880
fragaria_vesca	FvH4_3g44841 FvH4_3g44841 FvH4_3g44841
malus_domestica	MD03G1008000.v1.1 MD03G1008200.v1.1 MD11G1011600.v1.1 MD12G1151800.v1.1 MD14G1141900.v1.1
prunus_persica	Prupe.6G010000_v2.0.a1
pyrus_communis	pycom03g00820 pycom03g00840 pycom03g00850 pycom03g00880 pycom11g00770
rosa_chinensis	RchiOBHm_Chr5g0081551
rosa_laevigata	RLG00000036947
rosa_multiflora	Rmu_sc0002706.1_g000001 Rmu_sc0014139.1_g000005
rosa_roxburghii	Rroxscaffold_1G00002000
rosa_samantha	Rh5AG528600 Rh5CG576200 Rh5DG563500
rosa_wichuraiana	Rw5G049180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	112
Acc36I	ACCTGC	1 cut(s)	1264
Acc65I	GGTACC	1 cut(s)	871
AccB1I	GGYRCC	2 cut(s)	340, 871
AccB7I	CCANNNNNTGG	1 cut(s)	359
AccI	GTMKAC	2 cut(s)	237, 1380
AccII	CGCG	1 cut(s)	1010
AccIII	TCCGGA	1 cut(s)	246
AciI	CCGC	4 cut(s)	103, 132, 328, 1251
AclI	AACGTT	1 cut(s)	180
AcsI	RAATTY	1 cut(s)	1027
AcuI	CTGAAG	1 cut(s)	1282
AcyI	GRCGYC	2 cut(s)	341, 965
AfaI	GTAC	7 cut(s)	122, 272, 578, 873, 884, 1348, 1394
AfiI	CCNNNNNNNGG	5 cut(s)	202, 359, 730, 849, 1314
AgsI	TTSAA	5 cut(s)	460, 505, 639, 800, 896
AhdI	GACNNNNNGTC	1 cut(s)	77
AjnI	CCWGG	3 cut(s)	75, 195, 854
AluBI	AGCT	3 cut(s)	371, 512, 758
AluI	AGCT	3 cut(s)	371, 512, 758
Alw21I	GWGCWC	3 cut(s)	760, 1054, 1086
Alw26I	GTCTC	2 cut(s)	536, 984
Aor13HI	TCCGGA	1 cut(s)	246
AoxI	GGCC	2 cut(s)	693, 851
ApeKI	GCWGC	4 cut(s)	473, 1076, 1244, 1388
ApoI	RAATTY	1 cut(s)	1027
AseI	ATTAAT	1 cut(s)	399
Asp718I	GGTACC	1 cut(s)	871
AspLEI	GCGC	3 cut(s)	343, 723, 765
AspS9I	GGNCC	5 cut(s)	79, 463, 642, 852, 1148
AsuHPI	GGTGA	2 cut(s)	12, 960
AvaII	GGWCC	4 cut(s)	79, 463, 642, 1148
AxyI	CCTNAGG	1 cut(s)	1059
BaeI	ACNNNNGTAYC	2 cut(s)	863, 896
BanI	GGYRCC	2 cut(s)	340, 871
BanII	GRGCYC	1 cut(s)	760
BauI	CACGAG	1 cut(s)	1341
Bbv12I	GWGCWC	3 cut(s)	760, 1054, 1086
BbvI	GCAGC	4 cut(s)	485, 1063, 1256, 1375
BccI	CCATC	6 cut(s)	146, 170, 588, 847, 1139, 1353
BciT130I	CCWGG	3 cut(s)	77, 197, 856
BciVI	GTATCC	2 cut(s)	803, 1187
BclI	TGATCA	1 cut(s)	48
BcoDI	GTCTC	2 cut(s)	536, 984
BfaI	CTAG	1 cut(s)	1403
BfoI	RGCGCY	2 cut(s)	344, 724
BfuAI	ACCTGC	1 cut(s)	1264
BfuI	GTATCC	2 cut(s)	803, 1187
BisI	GCNGC	4 cut(s)	474, 1077, 1245, 1389
BlsI	GCNGC	4 cut(s)	475, 1078, 1246, 1390
Bme1390I	CCNGG	3 cut(s)	77, 197, 856
Bme18I	GGWCC	4 cut(s)	79, 463, 642, 1148
BmeRI	GACNNNNNGTC	1 cut(s)	77
BmgT120I	GGNCC	5 cut(s)	79, 463, 642, 852, 1148
BmiI	GGNNCC	3 cut(s)	342, 351, 873
BmrFI	CCNGG	3 cut(s)	77, 197, 856
BmsI	GCATC	2 cut(s)	691, 697
BpmI	CTGGAG	1 cut(s)	1093
BpuEI	CTTGAG	2 cut(s)	733, 943
BsaHI	GRCGYC	2 cut(s)	341, 965
BsaJI	CCNNGG	3 cut(s)	195, 196, 854
BsaWI	WCCGGW	1 cut(s)	246
BsaXI	ACNNNNNCTCC	4 cut(s)	12, 42, 113, 143
Bsc4I	CCNNNNNNNGG	5 cut(s)	202, 359, 730, 849, 1314
Bse1I	ACTGG	3 cut(s)	64, 353, 1354
Bse21I	CCTNAGG	1 cut(s)	1059
Bse3DI	GCAATG	3 cut(s)	603, 734, 868
BseAI	TCCGGA	1 cut(s)	246
BseBI	CCWGG	3 cut(s)	77, 197, 856
BseDI	CCNNGG	3 cut(s)	195, 196, 854
BseGI	GGATG	2 cut(s)	162, 839
BseLI	CCNNNNNNNGG	5 cut(s)	202, 359, 730, 849, 1314
BseMI	GCAATG	3 cut(s)	603, 734, 868
BseMII	CTCAG	2 cut(s)	1050, 1176
BseNI	ACTGG	3 cut(s)	64, 353, 1354
BseRI	GAGGAG	2 cut(s)	1044, 1096
BseXI	GCAGC	4 cut(s)	485, 1063, 1256, 1375
BsgI	GTGCAG	2 cut(s)	153, 1292
Bsh1236I	CGCG	1 cut(s)	1010
BshFI	GGCC	2 cut(s)	695, 853
BshNI	GGYRCC	2 cut(s)	340, 871
BsiHKAI	GWGCWC	3 cut(s)	760, 1054, 1086
BsiSI	CCGG	2 cut(s)	247, 387
BsiWI	CGTACG	1 cut(s)	120
BslFI	GGGAC	1 cut(s)	242
BslI	CCNNNNNNNGG	5 cut(s)	202, 359, 730, 849, 1314
BsmAI	GTCTC	2 cut(s)	536, 984
BsmFI	GGGAC	1 cut(s)	242
BsnI	GGCC	2 cut(s)	695, 853
Bsp1286I	GDGCHC	3 cut(s)	760, 1054, 1086
Bsp13I	TCCGGA	1 cut(s)	246
Bsp1407I	TGTACA	1 cut(s)	882
Bsp143I	GATC	2 cut(s)	48, 53
BspACI	CCGC	4 cut(s)	103, 132, 328, 1251
BspANI	GGCC	2 cut(s)	695, 853
BspCNI	CTCAG	2 cut(s)	1051, 1175
BspEI	TCCGGA	1 cut(s)	246
BspFNI	CGCG	1 cut(s)	1010
BspLI	GGNNCC	3 cut(s)	342, 351, 873
BspMI	ACCTGC	1 cut(s)	1264
BspT107I	GGYRCC	2 cut(s)	340, 871
BsrDI	GCAATG	3 cut(s)	603, 734, 868
BsrGI	TGTACA	1 cut(s)	882
BsrI	ACTGG	3 cut(s)	64, 353, 1354
BssECI	CCNNGG	3 cut(s)	195, 196, 854
BssMI	GATC	2 cut(s)	48, 53
BssNAI	GTATAC	1 cut(s)	1381
BssNI	GRCGYC	2 cut(s)	341, 965
BssSI	CACGAG	1 cut(s)	1341
Bst1107I	GTATAC	1 cut(s)	1381
Bst2BI	CACGAG	1 cut(s)	1341
Bst2UI	CCWGG	3 cut(s)	77, 197, 856
Bst4CI	ACNGT	4 cut(s)	46, 233, 334, 945
BstACI	GRCGYC	2 cut(s)	341, 965
BstAUI	TGTACA	1 cut(s)	882
BstC8I	GCNNGC	3 cut(s)	741, 963, 1134
BstDEI	CTNAG	2 cut(s)	1059, 1162
BstF5I	GGATG	2 cut(s)	162, 839
BstFNI	CGCG	1 cut(s)	1010
BstH2I	RGCGCY	2 cut(s)	344, 724
BstHHI	GCGC	3 cut(s)	343, 723, 765
BstKTI	GATC	2 cut(s)	51, 56
BstMAI	GTCTC	2 cut(s)	536, 984
BstMBI	GATC	2 cut(s)	48, 53
BstMWI	GCNNNNNNNGC	5 cut(s)	482, 701, 740, 800, 1250
BstNI	CCWGG	3 cut(s)	77, 197, 856
BstNSI	RCATGY	2 cut(s)	316, 1271
BstSCI	CCNGG	3 cut(s)	75, 195, 854
BstUI	CGCG	1 cut(s)	1010
BstV1I	GCAGC	4 cut(s)	485, 1063, 1256, 1375
BstXI	CCANNNNNNTGG	1 cut(s)	76
BstZ17I	GTATAC	1 cut(s)	1381
Bsu36I	CCTNAGG	1 cut(s)	1059
BsuI	GTATCC	2 cut(s)	803, 1187
BsuRI	GGCC	2 cut(s)	695, 853
BtgZI	GCGATG	1 cut(s)	1372
BtsCI	GGATG	2 cut(s)	162, 839
BtsI	GCAGTG	1 cut(s)	483
BtsIMutI	CAGTG	3 cut(s)	51, 238, 483
BveI	ACCTGC	1 cut(s)	1264
Cac8I	GCNNGC	3 cut(s)	741, 963, 1134
CfoI	GCGC	3 cut(s)	343, 723, 765
Cfr13I	GGNCC	5 cut(s)	79, 463, 642, 852, 1148
CseI	GACGC	2 cut(s)	954, 1311
Csp6I	GTAC	7 cut(s)	121, 271, 577, 872, 883, 1347, 1393
CviAII	CATG	5 cut(s)	35, 313, 1218, 1264, 1268
CviQI	GTAC	7 cut(s)	121, 271, 577, 872, 883, 1347, 1393
DdeI	CTNAG	2 cut(s)	1059, 1162
DinI	GGCGCC	1 cut(s)	342
DpnI	GATC	2 cut(s)	50, 55
DpnII	GATC	2 cut(s)	48, 53
DrdI	GACNNNNNNGTC	1 cut(s)	112
DriI	GACNNNNNGTC	1 cut(s)	77
DseDI	GACNNNNNNGTC	1 cut(s)	112
Eam1105I	GACNNNNNGTC	1 cut(s)	77
EciI	GGCGGA	2 cut(s)	118, 121
Ecl136II	GAGCTC	1 cut(s)	758
Eco147I	AGGCCT	1 cut(s)	695
Eco24I	GRGCYC	1 cut(s)	760
Eco47I	GGWCC	4 cut(s)	79, 463, 642, 1148
Eco53kI	GAGCTC	1 cut(s)	758
Eco57I	CTGAAG	1 cut(s)	1282
Eco81I	CCTNAGG	1 cut(s)	1059
EcoICRI	GAGCTC	1 cut(s)	758
EcoO109I	RGGNCCY	1 cut(s)	852
EcoRII	CCWGG	3 cut(s)	75, 195, 854
EcoT22I	ATGCAT	2 cut(s)	690, 1269
EcoT38I	GRGCYC	1 cut(s)	760
EgeI	GGCGCC	1 cut(s)	342
EheI	GGCGCC	1 cut(s)	342
FaeI	CATG	5 cut(s)	38, 316, 1221, 1267, 1271
FalI	AAGNNNNNCTT	2 cut(s)	859, 891
FaqI	GGGAC	1 cut(s)	242
FatI	CATG	5 cut(s)	34, 312, 1217, 1263, 1267
FauI	CCCGC	1 cut(s)	335
FauNDI	CATATG	1 cut(s)	805
FbaI	TGATCA	1 cut(s)	48
FblI	GTMKAC	2 cut(s)	237, 1380
Fnu4HI	GCNGC	4 cut(s)	474, 1077, 1245, 1389
FokI	GGATG	2 cut(s)	149, 826
FriOI	GRGCYC	1 cut(s)	760
Fsp4HI	GCNGC	4 cut(s)	474, 1077, 1245, 1389
FspBI	CTAG	1 cut(s)	1403
GlaI	GCGC	3 cut(s)	342, 722, 764
GluI	GCNGC	4 cut(s)	474, 1077, 1245, 1389
GsuI	CTGGAG	1 cut(s)	1093
HaeII	RGCGCY	2 cut(s)	344, 724
HaeIII	GGCC	2 cut(s)	695, 853
HapII	CCGG	2 cut(s)	247, 387
HgaI	GACGC	2 cut(s)	954, 1311
HhaI	GCGC	3 cut(s)	343, 723, 765
Hin1I	GRCGYC	2 cut(s)	341, 965
Hin1II	CATG	5 cut(s)	38, 316, 1221, 1267, 1271
Hin6I	GCGC	3 cut(s)	341, 721, 763
HinP1I	GCGC	3 cut(s)	341, 721, 763
HinfI	GANTC	5 cut(s)	72, 85, 106, 212, 1070
HpaII	CCGG	2 cut(s)	247, 387
HphI	GGTGA	2 cut(s)	12, 960
Hpy166II	GTNNAC	2 cut(s)	238, 1381
Hpy188I	TCNGA	6 cut(s)	53, 541, 681, 844, 1165, 1422
Hpy8I	GTNNAC	2 cut(s)	238, 1381
Hpy99I	CGWCG	1 cut(s)	1327
HpyAV	CCTTC	3 cut(s)	308, 454, 1306
HpyCH4III	ACNGT	4 cut(s)	46, 233, 334, 945
HpyCH4IV	ACGT	1 cut(s)	180
HpyF10VI	GCNNNNNNNGC	5 cut(s)	482, 701, 740, 800, 1250
HpyF3I	CTNAG	2 cut(s)	1059, 1162
HpySE526I	ACGT	1 cut(s)	180
Hsp92I	GRCGYC	2 cut(s)	341, 965
Hsp92II	CATG	5 cut(s)	38, 316, 1221, 1267, 1271
HspAI	GCGC	3 cut(s)	341, 721, 763
KasI	GGCGCC	1 cut(s)	340
Kpn2I	TCCGGA	1 cut(s)	246
KpnI	GGTACC	1 cut(s)	875
Ksp22I	TGATCA	1 cut(s)	48
Kzo9I	GATC	2 cut(s)	48, 53
LmnI	GCTCC	2 cut(s)	755, 1057
Lsp1109I	GCAGC	4 cut(s)	485, 1063, 1256, 1375
LweI	GCATC	2 cut(s)	691, 697
MaeI	CTAG	1 cut(s)	1403
MaeII	ACGT	1 cut(s)	180
MaeIII	GTNAC	2 cut(s)	899, 966
MalI	GATC	2 cut(s)	50, 55
MboI	GATC	2 cut(s)	48, 53
MboII	GAAGA	6 cut(s)	283, 293, 827, 1024, 1178, 1391
MhlI	GDGCHC	3 cut(s)	760, 1054, 1086
MluCI	AATT	2 cut(s)	886, 1027
Mly113I	GGCGCC	1 cut(s)	341
MlyI	GAGTC	4 cut(s)	66, 79, 100, 221
Mph1103I	ATGCAT	2 cut(s)	690, 1269
MroI	TCCGGA	1 cut(s)	246
MseI	TTAA	3 cut(s)	399, 657, 939
MslI	CAYNNNNRTG	1 cut(s)	1268
MspI	CCGG	2 cut(s)	247, 387
MspR9I	CCNGG	3 cut(s)	77, 197, 856
MvaI	CCWGG	3 cut(s)	77, 197, 856
MvnI	CGCG	1 cut(s)	1010
MwoI	GCNNNNNNNGC	5 cut(s)	482, 701, 740, 800, 1250
NarI	GGCGCC	1 cut(s)	341
NdeI	CATATG	1 cut(s)	805
NdeII	GATC	2 cut(s)	48, 53
NlaIII	CATG	5 cut(s)	38, 316, 1221, 1267, 1271
NlaIV	GGNNCC	3 cut(s)	342, 351, 873
NmuCI	GTSAC	2 cut(s)	899, 966
NsiI	ATGCAT	2 cut(s)	690, 1269
NspI	RCATGY	2 cut(s)	316, 1271
PasI	CCCWGGG	1 cut(s)	196
PceI	AGGCCT	1 cut(s)	695
PfeI	GAWTC	1 cut(s)	1070
Pfl23II	CGTACG	1 cut(s)	120
PflFI	GACNNNGTC	1 cut(s)	233
PflMI	CCANNNNNTGG	1 cut(s)	359
PkrI	GCNGC	4 cut(s)	475, 1078, 1246, 1390
PleI	GAGTC	4 cut(s)	66, 79, 100, 220
PluTI	GGCGCC	1 cut(s)	344
PpsI	GAGTC	4 cut(s)	66, 79, 100, 220
PshBI	ATTAAT	1 cut(s)	399
Psp124BI	GAGCTC	1 cut(s)	760
Psp1406I	AACGTT	1 cut(s)	180
Psp6I	CCWGG	3 cut(s)	75, 195, 854
PspGI	CCWGG	3 cut(s)	75, 195, 854
PspLI	CGTACG	1 cut(s)	120
PspN4I	GGNNCC	3 cut(s)	342, 351, 873
PspPI	GGNCC	5 cut(s)	79, 463, 642, 852, 1148
PsyI	GACNNNGTC	1 cut(s)	233
RsaI	GTAC	7 cut(s)	122, 272, 578, 873, 884, 1348, 1394
RsaNI	GTAC	7 cut(s)	121, 271, 577, 872, 883, 1347, 1393
RseI	CAYNNNNRTG	1 cut(s)	1268
SacI	GAGCTC	1 cut(s)	760
SaqAI	TTAA	3 cut(s)	399, 657, 939
SatI	GCNGC	4 cut(s)	474, 1077, 1245, 1389
Sau3AI	GATC	2 cut(s)	48, 53
Sau96I	GGNCC	5 cut(s)	79, 463, 642, 852, 1148
SchI	GAGTC	4 cut(s)	66, 79, 100, 221
ScrFI	CCNGG	3 cut(s)	77, 197, 856
SduI	GDGCHC	3 cut(s)	760, 1054, 1086
SfaNI	GCATC	2 cut(s)	691, 697
SfoI	GGCGCC	1 cut(s)	342
SinI	GGWCC	4 cut(s)	79, 463, 642, 1148
SmiMI	CAYNNNNRTG	1 cut(s)	1268
SmlI	CTYRAG	2 cut(s)	712, 922
SmoI	CTYRAG	2 cut(s)	712, 922
Sse9I	AATT	2 cut(s)	886, 1027
SseBI	AGGCCT	1 cut(s)	695
SsiI	CCGC	4 cut(s)	103, 132, 328, 1251
SspDI	GGCGCC	1 cut(s)	340
SspI	AATATT	1 cut(s)	975
SspMI	CTAG	1 cut(s)	1403
SstI	GAGCTC	1 cut(s)	760
StuI	AGGCCT	1 cut(s)	695
StyD4I	CCNGG	3 cut(s)	75, 195, 854
TaaI	ACNGT	4 cut(s)	46, 233, 334, 945
TaiI	ACGT	1 cut(s)	183
TaqI	TCGA	1 cut(s)	210
TasI	AATT	2 cut(s)	886, 1027
TatI	WGTACW	1 cut(s)	882
TfiI	GAWTC	1 cut(s)	1070
Tru1I	TTAA	3 cut(s)	399, 657, 939
Tru9I	TTAA	3 cut(s)	399, 657, 939
TscAI	CASTG	3 cut(s)	51, 238, 483
TseFI	GTSAC	2 cut(s)	899, 966
TseI	GCWGC	4 cut(s)	473, 1076, 1244, 1388
Tsp45I	GTSAC	2 cut(s)	899, 966
TspDTI	ATGAA	5 cut(s)	51, 179, 516, 992, 1349
TspGWI	ACGGA	1 cut(s)	1037
TspRI	CASTG	3 cut(s)	51, 238, 483
Tth111I	GACNNNGTC	1 cut(s)	233
Van91I	CCANNNNNTGG	1 cut(s)	359
VpaK11BI	GGWCC	4 cut(s)	79, 463, 642, 1148
VspI	ATTAAT	1 cut(s)	399
XapI	RAATTY	1 cut(s)	1027
XceI	RCATGY	2 cut(s)	316, 1271
XcmI	CCANNNNNNNNNTGG	1 cut(s)	587
XmiI	GTMKAC	2 cut(s)	237, 1380
XspI	CTAG	1 cut(s)	1403
Zsp2I	ATGCAT	2 cut(s)	690, 1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.