Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005270

Physical Location & Seq

Genomic Coordinates Forward (+)

24081135 .. 24084335

Gene Structure 3201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1161000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGTGTGGTCGATTCGATTTCTTGTGCTTTTTCTGGCATGCGTTCTTCCTGCTTTGGTTGAGAGCAGAGTCCGCCACTACAAGTTTAATGTGGTGCTGAGAAATAAAACTAAACTATGCTCAAGTAAGCCAATAGTTACCGTTAACGGCAAGTTCCCGGGGCCAACTCTCTATGCCAGGGAAGACGATACTGTGCTTGTCAAAGTCACCAACCATGTCAAGTATAACGTTTCCATTCACTGGCATGGTATCCGGCAACTACGAACCGGTTGGGCAGATGGTCCGGCATACATCACACAGTGTCCAATTCCCACAGGCCAAAGCTATGTGTACAACTTCACCATCACAGGGCAAAGAGGCACACTTCTTTGGCATGCACATATTCTCTGGCTAAGGGCAACTGTCCACGGTGCCCTTGTAATTTTGCCCAAGCGTGGGGTGCCTTATCCATTCCCAGCACCCCACAAGGAAGTAAATGTGGTATTAGGTGAATGGTGGAAATCAGATACTGAAACTGTGATCAATCAGGCTCTCAAATCTGGTATAGCACCTAATGTATCAGATGCTCACACTATTAACGGTCATCCAGGTGTCGTTCCAAATTGTTCTTCACAAGGTGGCTTTACATTGCCTGTGGAAAGCGGCAAGACTTACCTTCTACGAATCATCAATGCTGCGCTCAATGAGGAGCTCTTCTTCAAAATTGCAGGACACAACTTGACCATAGTGGAAGTAGATGCCACATATGTGAAACCCTTCAAGACGGACACCATTGTGATTGCCCCGGGACAAACCACCAATGCCCTAGTCACGGCCAATCAAAACTCCGGCAAGTACATGGTGGCAGCCTCGCCATTTATGGACTCTCCAATCGCTGTTGACAACCTCACGGCCACCGCCACTCTTCACTATACTGGCACACTTGCTAGCACACCTACAACGCTCACTAGCCCACCTCCCCAAAACGCAACCCAAGTTGCTAACAGCTTCATAAACTCTCTAAAAGCCCTAAATTCCAACAATTTTCCAGCCAAAGTCCCATTAAAAATCGATCACAATCTTTTGTTCACAGTTGGACTTGGGATTAATCCATGCCCCAGTTGCAAAGCTGGTAATGGAAGCAGAGTAGTGGGTAGTGTTAACAATGTCTCATTTGTTATGCCAACCACAGCTCTGCTTCAAGCACATGTTTTCAACAAAAGTGGGGTTTTTACCACGGATTTCCCCGGCAACCCGCCGACAGCTTTCAACTATTCTGGAGGTCCACCGGCTAATGCAAGCTTGGCAACTACAAATGGCACAAAGCTTTATAGGCTAGCTTATAACTCAACAGTTCAACTTGTGTTGCAAGACACTGGGACTATAGCCCCTGAGAACCACCCTATCCATTTGCATGGATTCAATTTCTTTTGTGTGGGTAGGGGCGTTGGGAACTATAACCCCAAGACGGATCCTAAGAAGTTCAATCTTGTTGATCCTGTTGAAAGGAACACAGCTAATATTCCGTCCGGCGGATGGACAGCTATTCGATTTGTCGCTGATAATCCAGGAGTTTGGTTCATGCATTGCCATTTGGAAGTGCACACAACATGGGGGCTTAAGATGGCATTCTTGGTGGACAATGGCAAAGGACCTAATCAATCTCTTCTTCCACCTCCAAAGGATCTCCCAAAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009832 GO:0009834 GO:0009987 GO:0016491 GO:0016722 GO:0019438 GO:0019748 GO:0042546 GO:0044085 GO:0044237 GO:0044249 GO:0044550 GO:0055114 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

560

Amino Acids

60.73

Weight (kDa)

9.39

Isoelectric Point (pI)

32.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	37 - 144	4e-40	Multicopper oxidase
Cu-oxidase	PF00394	157 - 306	4e-47	Multicopper oxidase
Cu-oxidase_2	PF07731	408 - 542	8.4e-43	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005270)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38080 AT5G01190 AT5G01190
fragaria_vesca	FvH4_6g11960 FvH4_7g23980
malus_domestica	MD04G1147700.v1.1 MD07G1213300.v1.1 MD12G1161000.v1.1
prunus_persica	Prupe.2G245800_v2.0.a1 Prupe.6G271500_v2.0.a1
pyrus_communis	pycom04g13330
rosa_chinensis	RchiOBHm_Chr1g0369321 RchiOBHm_Chr3g0463771
rosa_laevigata	RLG00000024758 RLG00000027137
rosa_multiflora	Rmu_sc0001482.1_g000010 Rmu_sc0003267.1_g000044 Rmu_sc0004796.1_g000014
rosa_roxburghii	Rroxscaffold_4G00287730 Rroxscaffold_6G00416320
rosa_rugosa	Rorug01G0349600 Rorug03G0067400
rosa_samantha	Rh1BG320800 Rh1CG334800 Rh3AG124600 Rh3BG129200 Rh3DG129900
rosa_wichuraiana	Rw1G031500 Rw3G010530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1323
AccB1I	GGYRCC	2 cut(s)	410, 439
AccB7I	CCANNNNNTGG	1 cut(s)	240
AciI	CCGC	5 cut(s)	73, 642, 897, 1235, 1512
AclI	AACGTT	1 cut(s)	228
AclWI	GGATC	4 cut(s)	1445, 1458, 1469, 1671
AcoI	YGGCCR	2 cut(s)	813, 891
AcsI	RAATTY	1 cut(s)	1012
AdeI	CACNNNGTG	2 cut(s)	300, 617
AfaI	GTAC	2 cut(s)	332, 836
AfiI	CCNNNNNNNGG	8 cut(s)	240, 348, 434, 435, 811, 1447, 1511, 1515
AflII	CTTAAG	1 cut(s)	1598
AflIII	ACRYGT	1 cut(s)	1186
AgeI	ACCGGT	1 cut(s)	266
AgsI	TTSAA	9 cut(s)	700, 760, 1181, 1195, 1249, 1337, 1402, 1465, 1484
AjnI	CCWGG	3 cut(s)	176, 586, 1546
AjuI	GAANNNNNNNTTGG	2 cut(s)	592, 624
AleI	CACNNNNGTG	2 cut(s)	746, 773
Alw21I	GWGCWC	2 cut(s)	693, 1584
Alw26I	GTCTC	1 cut(s)	1153
Alw44I	GTGCAC	1 cut(s)	1580
AlwI	GGATC	4 cut(s)	1445, 1458, 1469, 1671
AlwNI	CAGNNNCTG	1 cut(s)	509
Ama87I	CYCGRG	2 cut(s)	157, 784
AoxI	GGCC	4 cut(s)	161, 316, 813, 891
ApaLI	GTGCAC	1 cut(s)	1580
ApeKI	GCWGC	2 cut(s)	674, 845
ApoI	RAATTY	1 cut(s)	1012
AseI	ATTAAT	1 cut(s)	1086
AsiGI	ACCGGT	1 cut(s)	266
Asp700I	GAANNNNTTC	1 cut(s)	755
AspLEI	GCGC	1 cut(s)	679
AspS9I	GGNCC	4 cut(s)	161, 281, 1262, 1631
AsuC2I	CCSGG	5 cut(s)	158, 159, 785, 786, 1227
AsuHPI	GGTGA	3 cut(s)	199, 331, 500
AsuNHI	GCTAGC	2 cut(s)	926, 1315
AvaI	CYCGRG	2 cut(s)	157, 784
AvaII	GGWCC	3 cut(s)	281, 1262, 1631
BaeGI	GKGCMC	2 cut(s)	415, 1584
BamHI	GGATCC	1 cut(s)	1450
BanI	GGYRCC	2 cut(s)	410, 439
BanII	GRGCYC	1 cut(s)	693
BbsI	GAAGAC	1 cut(s)	189
Bbv12I	GWGCWC	2 cut(s)	693, 1584
BbvI	GCAGC	2 cut(s)	661, 857
BccI	CCATC	4 cut(s)	272, 350, 1509, 1597
BceAI	ACGGC	3 cut(s)	163, 828, 906
BciT130I	CCWGG	3 cut(s)	178, 588, 1548
BciVI	GTATCC	1 cut(s)	260
BclI	TGATCA	1 cut(s)	519
BcnI	CCSGG	5 cut(s)	158, 159, 785, 786, 1227
BcoDI	GTCTC	1 cut(s)	1153
BfaI	CTAG	4 cut(s)	806, 927, 948, 1316
BfmI	CTRYAG	1 cut(s)	1362
BfrI	CTTAAG	1 cut(s)	1598
BfuI	GTATCC	1 cut(s)	260
BisI	GCNGC	3 cut(s)	643, 675, 846
BlsI	GCNGC	3 cut(s)	644, 676, 847
Bme1390I	CCNGG	8 cut(s)	158, 159, 178, 588, 785, 786, 1227, 1548
Bme18I	GGWCC	3 cut(s)	281, 1262, 1631
BmeT110I	CYCGRG	2 cut(s)	157, 784
BmgT120I	GGNCC	4 cut(s)	161, 281, 1262, 1631
BmiI	GGNNCC	4 cut(s)	162, 412, 441, 1452
BmrFI	CCNGG	8 cut(s)	158, 159, 178, 588, 785, 786, 1227, 1548
BmrI	ACTGGG	2 cut(s)	1092, 1365
BmsI	GCATC	2 cut(s)	553, 727
BmtI	GCTAGC	2 cut(s)	930, 1319
BmuI	ACTGGG	2 cut(s)	1092, 1365
BpiI	GAAGAC	1 cut(s)	189
BpmI	CTGGAG	1 cut(s)	1278
Bpu10I	CCTNAGC	1 cut(s)	392
BpuEI	CTTGAG	1 cut(s)	106
BpuMI	CCSGG	5 cut(s)	158, 159, 785, 786, 1227
Bsa29I	ATCGAT	1 cut(s)	1050
BsaBI	GATNNNNATC	1 cut(s)	1056
BsaJI	CCNNGG	8 cut(s)	157, 158, 177, 406, 783, 784, 1215, 1225
BsaWI	WCCGGW	1 cut(s)	266
Bsc4I	CCNNNNNNNGG	8 cut(s)	240, 348, 434, 435, 811, 1447, 1511, 1515
Bse118I	RCCGGY	2 cut(s)	266, 1267
Bse1I	ACTGG	4 cut(s)	245, 919, 1098, 1360
Bse3DI	GCAATG	2 cut(s)	626, 1564
Bse8I	GATNNNNATC	1 cut(s)	1056
BseBI	CCWGG	3 cut(s)	178, 588, 1548
BseCI	ATCGAT	1 cut(s)	1050
BseDI	CCNNGG	8 cut(s)	157, 158, 177, 406, 783, 784, 1215, 1225
BseGI	GGATG	2 cut(s)	583, 1520
BseJI	GATNNNNATC	1 cut(s)	1056
BseLI	CCNNNNNNNGG	8 cut(s)	240, 348, 434, 435, 811, 1447, 1511, 1515
BseMI	GCAATG	2 cut(s)	626, 1564
BseMII	CTCAG	2 cut(s)	89, 1362
BseNI	ACTGG	4 cut(s)	245, 919, 1098, 1360
BseRI	GAGGAG	1 cut(s)	701
BseSI	GKGCMC	2 cut(s)	415, 1584
BseXI	GCAGC	2 cut(s)	661, 857
BseYI	CCCAGC	1 cut(s)	454
BshFI	GGCC	4 cut(s)	163, 318, 815, 893
BshNI	GGYRCC	2 cut(s)	410, 439
BshTI	ACCGGT	1 cut(s)	266
BshVI	ATCGAT	1 cut(s)	1050
BsiHKAI	GWGCWC	2 cut(s)	693, 1584
BsiHKCI	CYCGRG	2 cut(s)	157, 784
BsiSI	CCGG	9 cut(s)	158, 253, 267, 284, 785, 828, 1227, 1268, 1509
BslFI	GGGAC	3 cut(s)	801, 1022, 1372
BslI	CCNNNNNNNGG	8 cut(s)	240, 348, 434, 435, 811, 1447, 1511, 1515
BsmAI	GTCTC	1 cut(s)	1153
BsmFI	GGGAC	3 cut(s)	801, 1022, 1372
BsmI	GAATGC	1 cut(s)	1607
BsnI	GGCC	4 cut(s)	163, 318, 815, 893
BsoBI	CYCGRG	2 cut(s)	157, 784
Bsp1286I	GDGCHC	3 cut(s)	415, 693, 1584
Bsp1407I	TGTACA	1 cut(s)	330
Bsp143I	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
BspACI	CCGC	5 cut(s)	73, 642, 897, 1235, 1512
BspANI	GGCC	4 cut(s)	163, 318, 815, 893
BspCNI	CTCAG	2 cut(s)	90, 1363
BspDI	ATCGAT	1 cut(s)	1050
BspLI	GGNNCC	4 cut(s)	162, 412, 441, 1452
BspOI	GCTAGC	2 cut(s)	930, 1319
BspPI	GGATC	4 cut(s)	1445, 1458, 1469, 1671
BspQI	GCTCTTC	1 cut(s)	698
BspT107I	GGYRCC	2 cut(s)	410, 439
BspTI	CTTAAG	1 cut(s)	1598
BsrDI	GCAATG	2 cut(s)	626, 1564
BsrFI	RCCGGY	2 cut(s)	266, 1267
BsrGI	TGTACA	1 cut(s)	330
BsrI	ACTGG	4 cut(s)	245, 919, 1098, 1360
BssAI	RCCGGY	2 cut(s)	266, 1267
BssECI	CCNNGG	8 cut(s)	157, 158, 177, 406, 783, 784, 1215, 1225
BssMI	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
Bst2UI	CCWGG	3 cut(s)	178, 588, 1548
Bst4CI	ACNGT	9 cut(s)	142, 193, 300, 403, 410, 517, 581, 1072, 1333
Bst6I	CTCTTC	3 cut(s)	698, 909, 1650
BstAFI	CTTAAG	1 cut(s)	1598
BstAUI	TGTACA	1 cut(s)	330
BstC8I	GCNNGC	5 cut(s)	40, 375, 928, 1279, 1317
BstDEI	CTNAG	4 cut(s)	98, 392, 1371, 1455
BstDSI	CCRYGG	2 cut(s)	406, 1215
BstF5I	GGATG	2 cut(s)	583, 1520
BstHHI	GCGC	1 cut(s)	679
BstKTI	GATC	5 cut(s)	522, 1054, 1453, 1477, 1666
BstMAI	GTCTC	1 cut(s)	1153
BstMBI	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
BstMWI	GCNNNNNNNGC	5 cut(s)	72, 439, 1101, 1283, 1312
BstNI	CCWGG	3 cut(s)	178, 588, 1548
BstNSI	RCATGY	3 cut(s)	42, 377, 1190
BstSCI	CCNGG	8 cut(s)	156, 157, 176, 586, 783, 784, 1225, 1546
BstSFI	CTRYAG	1 cut(s)	1362
BstSLI	GKGCMC	2 cut(s)	415, 1584
BstV1I	GCAGC	2 cut(s)	661, 857
BstV2I	GAAGAC	1 cut(s)	189
BstX2I	RGATCY	2 cut(s)	1450, 1663
BstXI	CCANNNNNNTGG	2 cut(s)	1394, 1554
BstYI	RGATCY	2 cut(s)	1450, 1663
Bsu15I	ATCGAT	1 cut(s)	1050
BsuI	GTATCC	1 cut(s)	260
BsuRI	GGCC	4 cut(s)	163, 318, 815, 893
BsuTUI	ATCGAT	1 cut(s)	1050
BtgI	CCRYGG	2 cut(s)	406, 1215
BtsCI	GGATG	2 cut(s)	583, 1520
BtsIMutI	CAGTG	3 cut(s)	238, 305, 1353
Cac8I	GCNNGC	5 cut(s)	40, 375, 928, 1279, 1317
CaiI	CAGNNNCTG	1 cut(s)	509
CfoI	GCGC	1 cut(s)	679
Cfr10I	RCCGGY	2 cut(s)	266, 1267
Cfr13I	GGNCC	4 cut(s)	161, 281, 1262, 1631
Cfr9I	CCCGGG	2 cut(s)	157, 784
ClaI	ATCGAT	1 cut(s)	1050
Csp6I	GTAC	2 cut(s)	331, 835
CspAI	ACCGGT	1 cut(s)	266
CspCI	CAANNNNNGTGG	2 cut(s)	1183, 1218
CviQI	GTAC	2 cut(s)	331, 835
DdeI	CTNAG	4 cut(s)	98, 392, 1371, 1455
DpnI	GATC	5 cut(s)	521, 1053, 1452, 1476, 1665
DpnII	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
DraIII	CACNNNGTG	2 cut(s)	300, 617
EaeI	YGGCCR	2 cut(s)	813, 891
Eam1104I	CTCTTC	3 cut(s)	698, 909, 1650
EarI	CTCTTC	3 cut(s)	698, 909, 1650
EciI	GGCGGA	2 cut(s)	62, 1527
Ecl136II	GAGCTC	1 cut(s)	691
Eco24I	GRGCYC	1 cut(s)	693
Eco47I	GGWCC	3 cut(s)	281, 1262, 1631
Eco53kI	GAGCTC	1 cut(s)	691
Eco88I	CYCGRG	2 cut(s)	157, 784
EcoICRI	GAGCTC	1 cut(s)	691
EcoO109I	RGGNCCY	1 cut(s)	1631
EcoRII	CCWGG	3 cut(s)	176, 586, 1546
EcoT22I	ATGCAT	1 cut(s)	1566
EcoT38I	GRGCYC	1 cut(s)	693
FaqI	GGGAC	3 cut(s)	801, 1022, 1372
FauI	CCCGC	1 cut(s)	1242
FauNDI	CATATG	1 cut(s)	745
FbaI	TGATCA	1 cut(s)	519
Fnu4HI	GCNGC	3 cut(s)	643, 675, 846
FokI	GGATG	2 cut(s)	570, 1527
FriOI	GRGCYC	1 cut(s)	693
Fsp4HI	GCNGC	3 cut(s)	643, 675, 846
FspBI	CTAG	4 cut(s)	806, 927, 948, 1316
GlaI	GCGC	1 cut(s)	678
GluI	GCNGC	3 cut(s)	643, 675, 846
GsaI	CCCAGC	1 cut(s)	458
GsuI	CTGGAG	1 cut(s)	1278
HaeIII	GGCC	4 cut(s)	163, 318, 815, 893
HapII	CCGG	9 cut(s)	158, 253, 267, 284, 785, 828, 1227, 1268, 1509
HhaI	GCGC	1 cut(s)	679
Hin6I	GCGC	1 cut(s)	677
HinP1I	GCGC	1 cut(s)	677
HincII	GTYRAC	3 cut(s)	145, 880, 1141
HindII	GTYRAC	3 cut(s)	145, 880, 1141
HindIII	AAGCTT	2 cut(s)	1279, 1304
HinfI	GANTC	5 cut(s)	13, 69, 663, 863, 1398
HpaI	GTTAAC	2 cut(s)	145, 1141
HpaII	CCGG	9 cut(s)	158, 253, 267, 284, 785, 828, 1227, 1268, 1509
HphI	GGTGA	3 cut(s)	199, 331, 500
Hpy166II	GTNNAC	9 cut(s)	145, 331, 406, 880, 1068, 1141, 1265, 1582, 1618
Hpy188I	TCNGA	2 cut(s)	505, 562
Hpy188III	TCNNGA	2 cut(s)	760, 1257
Hpy8I	GTNNAC	9 cut(s)	145, 331, 406, 880, 1068, 1141, 1265, 1582, 1618
HpyAV	CCTTC	2 cut(s)	665, 766
HpyCH4III	ACNGT	9 cut(s)	142, 193, 300, 403, 410, 517, 581, 1072, 1333
HpyCH4IV	ACGT	1 cut(s)	228
HpyCH4V	TGCA	8 cut(s)	377, 707, 1104, 1277, 1348, 1393, 1564, 1582
HpyF10VI	GCNNNNNNNGC	5 cut(s)	72, 439, 1101, 1283, 1312
HpyF3I	CTNAG	4 cut(s)	98, 392, 1371, 1455
HpySE526I	ACGT	1 cut(s)	228
HspAI	GCGC	1 cut(s)	677
Ksp22I	TGATCA	1 cut(s)	519
KspAI	GTTAAC	2 cut(s)	145, 1141
Kzo9I	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
LguI	GCTCTTC	1 cut(s)	698
LmnI	GCTCC	1 cut(s)	688
Lsp1109I	GCAGC	2 cut(s)	661, 857
LweI	GCATC	2 cut(s)	553, 727
MaeI	CTAG	4 cut(s)	806, 927, 948, 1316
MaeII	ACGT	1 cut(s)	228
MaeIII	GTNAC	3 cut(s)	136, 205, 808
MalI	GATC	5 cut(s)	521, 1053, 1452, 1476, 1665
MboI	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
MboII	GAAGA	8 cut(s)	38, 194, 600, 685, 688, 896, 1637, 1640
MflI	RGATCY	2 cut(s)	1450, 1663
MhlI	GDGCHC	3 cut(s)	415, 693, 1584
MluCI	AATT	7 cut(s)	306, 420, 601, 702, 1012, 1021, 1402
MlyI	GAGTC	2 cut(s)	78, 857
MmeI	TCCRAC	2 cut(s)	1041, 1054
MnlI	CCTC	7 cut(s)	350, 679, 859, 896, 966, 1253, 1665
Mph1103I	ATGCAT	1 cut(s)	1566
MroXI	GAANNNNTTC	1 cut(s)	755
MseI	TTAA	8 cut(s)	87, 144, 576, 1043, 1086, 1140, 1599, 1678
MslI	CAYNNNNRTG	5 cut(s)	243, 588, 746, 773, 1392
MspCI	CTTAAG	1 cut(s)	1598
MspI	CCGG	9 cut(s)	158, 253, 267, 284, 785, 828, 1227, 1268, 1509
MspR9I	CCNGG	8 cut(s)	158, 159, 178, 588, 785, 786, 1227, 1548
Mva1269I	GAATGC	1 cut(s)	1607
MvaI	CCWGG	3 cut(s)	178, 588, 1548
MwoI	GCNNNNNNNGC	5 cut(s)	72, 439, 1101, 1283, 1312
NciI	CCSGG	5 cut(s)	158, 159, 785, 786, 1227
NdeI	CATATG	1 cut(s)	745
NdeII	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
NheI	GCTAGC	2 cut(s)	926, 1315
NlaIV	GGNNCC	4 cut(s)	162, 412, 441, 1452
NmuCI	GTSAC	2 cut(s)	205, 808
NsiI	ATGCAT	1 cut(s)	1566
NspI	RCATGY	3 cut(s)	42, 377, 1190
OliI	CACNNNNGTG	2 cut(s)	746, 773
PaeI	GCATGC	2 cut(s)	42, 377
PciI	ACATGT	1 cut(s)	1186
PciSI	GCTCTTC	1 cut(s)	698
PctI	GAATGC	1 cut(s)	1607
PdmI	GAANNNNTTC	1 cut(s)	755
PfeI	GAWTC	3 cut(s)	13, 663, 1398
PflMI	CCANNNNNTGG	1 cut(s)	240
PfoI	TCCNGGA	1 cut(s)	1546
PinAI	ACCGGT	1 cut(s)	266
PkrI	GCNGC	3 cut(s)	644, 676, 847
PleI	GAGTC	2 cut(s)	77, 857
PpsI	GAGTC	2 cut(s)	77, 857
PpuMI	RGGWCCY	1 cut(s)	1631
PscI	ACATGT	1 cut(s)	1186
PshBI	ATTAAT	1 cut(s)	1086
PsiI	TTATAA	1 cut(s)	1323
Psp124BI	GAGCTC	1 cut(s)	693
Psp1406I	AACGTT	1 cut(s)	228
Psp5II	RGGWCCY	1 cut(s)	1631
Psp6I	CCWGG	3 cut(s)	176, 586, 1546
PspFI	CCCAGC	1 cut(s)	454
PspGI	CCWGG	3 cut(s)	176, 586, 1546
PspN4I	GGNNCC	4 cut(s)	162, 412, 441, 1452
PspPI	GGNCC	4 cut(s)	161, 281, 1262, 1631
PspPPI	RGGWCCY	1 cut(s)	1631
PstNI	CAGNNNCTG	1 cut(s)	509
PsuI	RGATCY	2 cut(s)	1450, 1663
RsaI	GTAC	2 cut(s)	332, 836
RsaNI	GTAC	2 cut(s)	331, 835
RseI	CAYNNNNRTG	5 cut(s)	243, 588, 746, 773, 1392
SacI	GAGCTC	1 cut(s)	693
SapI	GCTCTTC	1 cut(s)	698
SaqAI	TTAA	8 cut(s)	87, 144, 576, 1043, 1086, 1140, 1599, 1678
SatI	GCNGC	3 cut(s)	643, 675, 846
Sau3AI	GATC	5 cut(s)	519, 1051, 1450, 1474, 1663
Sau96I	GGNCC	4 cut(s)	161, 281, 1262, 1631
SchI	GAGTC	2 cut(s)	78, 857
ScrFI	CCNGG	8 cut(s)	158, 159, 178, 588, 785, 786, 1227, 1548
SduI	GDGCHC	3 cut(s)	415, 693, 1584
SfaNI	GCATC	2 cut(s)	553, 727
SfcI	CTRYAG	1 cut(s)	1362
SinI	GGWCC	3 cut(s)	281, 1262, 1631
SmaI	CCCGGG	2 cut(s)	159, 786
SmiMI	CAYNNNNRTG	5 cut(s)	243, 588, 746, 773, 1392
SmlI	CTYRAG	2 cut(s)	121, 1598
SmoI	CTYRAG	2 cut(s)	121, 1598
SphI	GCATGC	2 cut(s)	42, 377
Sse9I	AATT	7 cut(s)	306, 420, 601, 702, 1012, 1021, 1402
SsiI	CCGC	5 cut(s)	73, 642, 897, 1235, 1512
SspI	AATATT	1 cut(s)	1501
SspMI	CTAG	4 cut(s)	806, 927, 948, 1316
SstI	GAGCTC	1 cut(s)	693
StyD4I	CCNGG	8 cut(s)	156, 157, 176, 586, 783, 784, 1225, 1546
TaaI	ACNGT	9 cut(s)	142, 193, 300, 403, 410, 517, 581, 1072, 1333
TaiI	ACGT	1 cut(s)	231
TaqI	TCGA	4 cut(s)	11, 16, 1050, 1528
TasI	AATT	7 cut(s)	306, 420, 601, 702, 1012, 1021, 1402
TatI	WGTACW	2 cut(s)	330, 834
TauI	GCSGC	1 cut(s)	645
TfiI	GAWTC	3 cut(s)	13, 663, 1398
Tru1I	TTAA	8 cut(s)	87, 144, 576, 1043, 1086, 1140, 1599, 1678
Tru9I	TTAA	8 cut(s)	87, 144, 576, 1043, 1086, 1140, 1599, 1678
TscAI	CASTG	3 cut(s)	245, 305, 1360
TseFI	GTSAC	2 cut(s)	205, 808
TseI	GCWGC	2 cut(s)	674, 845
Tsp45I	GTSAC	2 cut(s)	205, 808
TspDTI	ATGAA	2 cut(s)	979, 1549
TspGWI	ACGGA	4 cut(s)	779, 1232, 1463, 1494
TspMI	CCCGGG	2 cut(s)	157, 784
TspRI	CASTG	3 cut(s)	245, 305, 1360
Van91I	CCANNNNNTGG	1 cut(s)	240
Vha464I	CTTAAG	1 cut(s)	1598
VneI	GTGCAC	1 cut(s)	1580
VpaK11BI	GGWCC	3 cut(s)	281, 1262, 1631
VspI	ATTAAT	1 cut(s)	1086
XapI	RAATTY	1 cut(s)	1012
XceI	RCATGY	3 cut(s)	42, 377, 1190
XmaI	CCCGGG	2 cut(s)	157, 784
XmnI	GAANNNNTTC	1 cut(s)	755
XspI	CTAG	4 cut(s)	806, 927, 948, 1316
Zsp2I	ATGCAT	1 cut(s)	1566

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.