pycom04g13330

Belongs to the multicopper oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005270
Physical Location & Seq
Forward (+)
16297384 .. 16298438
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13330.1

Sequence Viewer

Length: 843 bp
ATGGTGGCAGCCTCGCCATTTATGGACTCTCCAATCGCCGTTGACAACCTCACCGCCACCGCCACTCTTCACTATACTGGCACACTTGCTACCACACCCACAACCCTCACTAGCGCACCTCCCCAAAACGCAACCCAAGTTGCTAACAGCTTCATAAACTCTCTCAAAGCCCTAAATTCCAAAAAATTTCCAGCCAAAGTCCCTTTAAAAATCGATCACAATCTTCTGTTCACAGTTGGACTTGGGATTAATTCATGCCCTAGTTGCAAAGCTGCAAACGGAAGCAGAGTAGTGGCTAGTGTTAACAATGTCACATTTGTTATGCCAACCACAGCTCTCCTTCAAGCACATTTTTTCAACAAAAGTGGGGTTTTTACCACGGATTTCCCCGGAAACCCACCAACGGCTTTCAACTTTTCTGGAGGTCTACCGGCAAATGCAAGCTTGGCAACTACAAGTGGGACAAAGCTTTATAGGCTAGCTTATAACTCAACAGTTCAAATTGTGTTGCAAGACACTGGGACTGTAGCCCCTGAGAACCACCCTGTCCATTTGCATGGATTCAATTTCTTTTCAGTGGGTAGGGGAATTGGGAATTATAACCCCAAGATGGATCCGAAGAAGTTCAATCTTGTTGATCCCGTTGAAAGGAACACAGCTAATGTTCCGACCGGCGGATGGGTAGCTATCCGATTTATCGCAGATAATCCAGGGGTTTGGTTCATGCATTGCCATTTGGAAGTGCACACAACATGGGGGCTTAAGATGGCATTCTTGGTGGACAATGGCAAAGGACCTAATCAATCTCTTCTTCCACCTCCAAAGGATCTCCCAAAATGTTAA

Protein Analysis

281

Amino Acids

29.92

Weight (kDa)

9.32

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 129 - 263 8.2e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 486, 600
AccI GTMKAC 1 cut(s) 427
AciI CCGC 3 cut(s) 54, 60, 675
AclWI GGATC 4 cut(s) 608, 621, 632, 834
AcsI RAATTY 2 cut(s) 175, 185
AfiI CCNNNNNNNGG 5 cut(s) 403, 610, 648, 674, 678
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 7 cut(s) 344, 358, 412, 500, 565, 628, 647
AjnI CCWGG 1 cut(s) 709
AluBI AGCT 8 cut(s) 150, 272, 335, 444, 469, 482, 659, 686
AluI AGCT 8 cut(s) 150, 272, 335, 444, 469, 482, 659, 686
Alw21I GWGCWC 1 cut(s) 747
Alw44I GTGCAC 1 cut(s) 743
AlwI GGATC 4 cut(s) 608, 621, 632, 834
ApaLI GTGCAC 1 cut(s) 743
ApeKI GCWGC 2 cut(s) 8, 272
ApoI RAATTY 2 cut(s) 175, 185
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 623
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 794
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 1 cut(s) 43
AsuNHI GCTAGC 1 cut(s) 478
AvaII GGWCC 1 cut(s) 794
BaeGI GKGCMC 1 cut(s) 747
BamHI GGATCC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 2 cut(s) 20, 259
BccI CCATC 3 cut(s) 604, 672, 760
BceAI ACGGC 2 cut(s) 23, 420
BciT130I CCWGG 1 cut(s) 711
BcnI CCSGG 1 cut(s) 390
BfaI CTAG 4 cut(s) 111, 261, 297, 479
BfmI CTRYAG 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 2 cut(s) 9, 273
BlsI GCNGC 2 cut(s) 10, 274
Bme1390I CCNGG 2 cut(s) 390, 711
Bme18I GGWCC 1 cut(s) 794
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 1 cut(s) 615
BmrFI CCNGG 2 cut(s) 390, 711
BmrI ACTGGG 1 cut(s) 528
BmtI GCTAGC 1 cut(s) 482
BmuI ACTGGG 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 441
BpuMI CCSGG 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 378, 388, 710
Bsc4I CCNNNNNNNGG 5 cut(s) 403, 610, 648, 674, 678
Bse118I RCCGGY 2 cut(s) 430, 671
Bse1I ACTGG 2 cut(s) 82, 523
Bse3DI GCAATG 1 cut(s) 727
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 1 cut(s) 711
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 378, 388, 710
BseGI GGATG 1 cut(s) 683
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 5 cut(s) 403, 610, 648, 674, 678
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 2 cut(s) 82, 523
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 2 cut(s) 20, 259
Bsh1285I CGRYCG 1 cut(s) 672
BshVI ATCGAT 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 747
BsiSI CCGG 3 cut(s) 390, 431, 672
BslFI GGGAC 3 cut(s) 185, 475, 535
BslI CCNNNNNNNGG 5 cut(s) 403, 610, 648, 674, 678
BsmFI GGGAC 3 cut(s) 185, 475, 535
BsmI GAATGC 1 cut(s) 770
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 4 cut(s) 214, 613, 637, 826
BspACI CCGC 3 cut(s) 54, 60, 675
BspCNI CTCAG 1 cut(s) 526
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 615
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 4 cut(s) 608, 621, 632, 834
BspTI CTTAAG 1 cut(s) 761
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 2 cut(s) 430, 671
BsrI ACTGG 2 cut(s) 82, 523
BssAI RCCGGY 2 cut(s) 430, 671
BssECI CCNNGG 3 cut(s) 378, 388, 710
BssMI GATC 4 cut(s) 214, 613, 637, 826
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 3 cut(s) 235, 496, 526
Bst6I CTCTTC 2 cut(s) 72, 813
BstAFI CTTAAG 1 cut(s) 761
BstC8I GCNNGC 2 cut(s) 442, 480
BstDEI CTNAG 1 cut(s) 534
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 683
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 217, 616, 640, 829
BstMBI GATC 4 cut(s) 214, 613, 637, 826
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 3 cut(s) 264, 446, 475
BstNI CCWGG 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 388, 709
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 747
BstV1I GCAGC 2 cut(s) 20, 259
BstX2I RGATCY 2 cut(s) 613, 826
BstXI CCANNNNNNTGG 2 cut(s) 557, 717
BstYI RGATCY 2 cut(s) 613, 826
Bsu15I ATCGAT 1 cut(s) 213
BsuTUI ATCGAT 1 cut(s) 213
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 683
BtsIMutI CAGTG 2 cut(s) 516, 582
Cac8I GCNNGC 2 cut(s) 442, 480
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 2 cut(s) 430, 671
Cfr13I GGNCC 1 cut(s) 794
ClaI ATCGAT 1 cut(s) 213
CspCI CAANNNNNGTGG 2 cut(s) 346, 381
CviAII CATG 4 cut(s) 255, 557, 724, 753
DdeI CTNAG 1 cut(s) 534
DpnI GATC 4 cut(s) 216, 615, 639, 828
DpnII GATC 4 cut(s) 214, 613, 637, 826
DraI TTTAAA 1 cut(s) 207
Eam1104I CTCTTC 2 cut(s) 72, 813
EarI CTCTTC 2 cut(s) 72, 813
EciI GGCGGA 1 cut(s) 690
Eco47I GGWCC 1 cut(s) 794
EcoO109I RGGNCCY 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 709
EcoT22I ATGCAT 1 cut(s) 729
FaeI CATG 4 cut(s) 258, 560, 727, 756
FaqI GGGAC 3 cut(s) 185, 475, 535
FatI CATG 4 cut(s) 254, 556, 723, 752
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 2 cut(s) 9, 273
FokI GGATG 1 cut(s) 690
Fsp4HI GCNGC 2 cut(s) 9, 273
FspBI CTAG 4 cut(s) 111, 261, 297, 479
GlaI GCGC 1 cut(s) 115
GluI GCNGC 2 cut(s) 9, 273
GsuI CTGGAG 1 cut(s) 441
HapII CCGG 3 cut(s) 390, 431, 672
HhaI GCGC 1 cut(s) 116
Hin1II CATG 4 cut(s) 258, 560, 727, 756
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 2 cut(s) 43, 304
HindII GTYRAC 2 cut(s) 43, 304
HindIII AAGCTT 2 cut(s) 442, 467
HinfI GANTC 2 cut(s) 26, 561
HpaI GTTAAC 1 cut(s) 304
HpaII CCGG 3 cut(s) 390, 431, 672
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 6 cut(s) 43, 231, 304, 428, 745, 781
Hpy188I TCNGA 3 cut(s) 618, 669, 692
Hpy188III TCNNGA 1 cut(s) 420
Hpy8I GTNNAC 6 cut(s) 43, 231, 304, 428, 745, 781
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 3 cut(s) 235, 496, 526
HpyCH4V TGCA 7 cut(s) 267, 275, 440, 511, 556, 727, 745
HpyF10VI GCNNNNNNNGC 3 cut(s) 264, 446, 475
HpyF3I CTNAG 1 cut(s) 534
Hsp92II CATG 4 cut(s) 258, 560, 727, 756
HspAI GCGC 1 cut(s) 114
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 4 cut(s) 214, 613, 637, 826
Lsp1109I GCAGC 2 cut(s) 20, 259
MaeI CTAG 4 cut(s) 111, 261, 297, 479
MaeIII GTNAC 1 cut(s) 310
MalI GATC 4 cut(s) 216, 615, 639, 828
MboI GATC 4 cut(s) 214, 613, 637, 826
MboII GAAGA 5 cut(s) 59, 215, 631, 800, 803
MflI RGATCY 2 cut(s) 613, 826
MhlI GDGCHC 1 cut(s) 747
MluCI AATT 7 cut(s) 175, 185, 250, 501, 565, 588, 595
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 217, 692
MnlI CCTC 6 cut(s) 22, 59, 116, 129, 416, 828
Mph1103I ATGCAT 1 cut(s) 729
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 5 cut(s) 206, 249, 303, 762, 841
MslI CAYNNNNRTG 1 cut(s) 555
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 3 cut(s) 390, 431, 672
MspR9I CCNGG 2 cut(s) 390, 711
Mva1269I GAATGC 1 cut(s) 770
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 3 cut(s) 264, 446, 475
NciI CCSGG 1 cut(s) 390
NdeII GATC 4 cut(s) 214, 613, 637, 826
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 4 cut(s) 258, 560, 727, 756
NlaIV GGNNCC 1 cut(s) 615
NmuCI GTSAC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 729
PctI GAATGC 1 cut(s) 770
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 1 cut(s) 561
PkrI GCNGC 2 cut(s) 10, 274
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 794
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 2 cut(s) 486, 600
Psp5II RGGWCCY 1 cut(s) 794
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 1 cut(s) 615
PspPI GGNCC 1 cut(s) 794
PspPPI RGGWCCY 1 cut(s) 794
PsuI RGATCY 2 cut(s) 613, 826
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 5 cut(s) 206, 249, 303, 762, 841
SatI GCNGC 2 cut(s) 9, 273
Sau3AI GATC 4 cut(s) 214, 613, 637, 826
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 390, 711
SduI GDGCHC 1 cut(s) 747
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 7 cut(s) 175, 185, 250, 501, 565, 588, 595
SsiI CCGC 3 cut(s) 54, 60, 675
SspMI CTAG 4 cut(s) 111, 261, 297, 479
StyD4I CCNGG 2 cut(s) 388, 709
TaaI ACNGT 3 cut(s) 235, 496, 526
TaqI TCGA 1 cut(s) 213
TasI AATT 7 cut(s) 175, 185, 250, 501, 565, 588, 595
TfiI GAWTC 1 cut(s) 561
Tru1I TTAA 5 cut(s) 206, 249, 303, 762, 841
Tru9I TTAA 5 cut(s) 206, 249, 303, 762, 841
TscAI CASTG 2 cut(s) 523, 582
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 8, 272
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 3 cut(s) 142, 243, 712
TspGWI ACGGA 2 cut(s) 294, 395
TspRI CASTG 2 cut(s) 523, 582
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 794
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 2 cut(s) 175, 185
XmiI GTMKAC 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 4 cut(s) 111, 261, 297, 479
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.