MD12G1184200.v1.1
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007643
Physical Location & Seq
Reverse (-)
26446186 .. 26449672
3487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1184200.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGCGGCTCTTCCTCCACCGTACGACCTCTAATTCCTCACCGCCTCTTCATCGTTCGCTATCGCCGCCGTTCCTTCTCCGCCAAGTTCTTCCGCCGCGGAAGTGGATGAAGAATTGGAGGAACGACGGTCGTACGACGAGCTCTGAAGCGCCGGGTTCTCCCGTCACTCCCGGTGGCAATTCCGTAATTCGATCTGATTCCGTGACCAGCTGTGGCTCTTCTTCAAATACTTACGATGGTTTTGTGAGTGGAAATGGCGGAAACTCAAACGAAAACTGGCAGATGATAAGGGTTGGAAGTTTTTCAATTGATTACAAGATTTTTGATGTCCCATCTGGTATTAGATCAACGCAACTTGTTCCCAAGTCTGCATTAGATCCTACTGATAAGGCACACTGCTACTCTCTCAGTTCGTTTATTCATTTCAATAAAAGGAAGTATCGAATCGATTTAAGATCAAAGCTAGTTAATATGGAAGATGCTGCTACAAGTTCAAGCTCTAAGAATATAAGTAAACTTCCACATGAAAGCTCAAATATGTCAGATTGTCAAAGAGATAACGATATATATTCATCTTTACCTACAGAATTTGGGAAAAAAAGAAAGCATGGCCTCCCAGAAAAATCTCAGTCCGCACCAAGAAATATGAAATATGTACCTGTTAGCAAGAACTCATATTGGTCTAAGGGTTCGCAGGAACCGTTTGATATATGCATGTCTCTGACCAGAAGTTCTGCACCTAAAGATTCTTGGCATGCAAGAAAGATAGAAAACCTGAATGAAGAGGAGGACATGGTGGAATTCGAAAATCCAGCACACACGGAACTCACAAGTTCAATAGTACTACTGAGGTCTGGGATGGTATTGTTGAAACATTTCGTCACCCTCAGTGAACAGGTTGAAATAGTGAAAAAATGCCGGGAGCTCGGTTTAAGTCCTGGAGGCTTTTATCAGCCAGGTTACCATGATGGAGCAAAACTTCGTCTACAGATGATGTGCCTTGGCTGGGACTGGGATCCTCAGACAAGAAAGTACGGAGATAGGCGAACAGTAGATGGTACTCGGCCTCCTGGTATTCCTAAGGAATTTAGTTTGCTGGTAAAAAGAGCGATTGATGAGGCACATGCCCACATTAAAGAGGAACTCAGAGTTAGCAGAGCTGAAGAAATACTCCCTTCAATGACTCCTGATATATGCATTGCCAACTTTTATACAACAACTGGCCGACTTGGTCTTCATCAGGATCGCGATGAAAGCACAAAGTCTCTCCATGACGGATTGCCTGTGGTCTCCATCTCTATAGGTGATTCAGCAGACTTCCTCTATGGAGATCAGAGAGATATTGCAAAGGCAGAGAATGTTGTTTTGGAATCAGGTGACGTGCTGATATTTGGAGGACCTTCGAGGCATATTTTTCATAGCGTGACATCCATCCTACCGGACTCAGCCCCAAGGAGTTTGTTGGAAGCAACCAGCCTTCGTCCTGGACGTCTCAATCTTACCTTCAGACAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

506

Amino Acids

56.54

Weight (kDa)

8.88

Isoelectric Point (pI)

58.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 286 - 503 2.3e-46 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1492
AccI GTMKAC 1 cut(s) 985
AccII CGCG 2 cut(s) 97, 1248
AciI CCGC 9 cut(s) 4, 41, 65, 79, 92, 95, 97, 258, 633
AclWI GGATC 4 cut(s) 371, 1010, 1023, 1251
AcoI YGGCCR 1 cut(s) 1222
AcsI RAATTY 3 cut(s) 587, 800, 1085
AcuI CTGAAG 3 cut(s) 165, 1182, 1489
AcyI GRCGYC 1 cut(s) 1489
AfaI GTAC 6 cut(s) 22, 133, 657, 843, 1034, 1060
AfiI CCNNNNNNNGG 1 cut(s) 1006
AgsI TTSAA 8 cut(s) 225, 306, 427, 495, 837, 871, 902, 1179
AjiI CACGTC 1 cut(s) 1381
AjnI CCWGG 4 cut(s) 937, 955, 1069, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 1349, 1381
AluBI AGCT 7 cut(s) 141, 210, 463, 498, 531, 925, 1160
AluI AGCT 7 cut(s) 141, 210, 463, 498, 531, 925, 1160
Alw21I GWGCWC 2 cut(s) 143, 927
Alw26I GTCTC 4 cut(s) 723, 1269, 1294, 1496
AlwI GGATC 4 cut(s) 371, 1010, 1023, 1251
AoxI GGCC 3 cut(s) 610, 1064, 1222
ApeKI GCWGC 1 cut(s) 482
ApoI RAATTY 3 cut(s) 587, 800, 1085
Asp700I GAANNNNTTC 2 cut(s) 301, 875
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 1397
AsuC2I CCSGG 3 cut(s) 153, 171, 920
AsuHPI GGTGA 4 cut(s) 30, 874, 1316, 1388
AsuII TTCGAA 1 cut(s) 804
AvaII GGWCC 1 cut(s) 1397
AxyI CCTNAGG 1 cut(s) 1080
BamHI GGATCC 1 cut(s) 1015
BanII GRGCYC 2 cut(s) 143, 927
BbsI GAAGAC 1 cut(s) 1226
Bbv12I GWGCWC 2 cut(s) 143, 927
BbvI GCAGC 1 cut(s) 469
BccI CCATC 7 cut(s) 230, 340, 853, 962, 1049, 1301, 1439
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 4 cut(s) 939, 957, 1071, 1485
BcnI CCSGG 3 cut(s) 153, 171, 920
BcoDI GTCTC 4 cut(s) 723, 1269, 1294, 1496
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 3 cut(s) 582, 986, 1299
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 4 cut(s) 5, 65, 95, 483
BlsI GCNGC 4 cut(s) 6, 66, 96, 484
BmcAI AGTACT 1 cut(s) 843
Bme1390I CCNGG 7 cut(s) 153, 171, 920, 939, 957, 1071, 1485
Bme18I GGWCC 1 cut(s) 1397
BmgBI CACGTC 1 cut(s) 1381
BmgT120I GGNCC 1 cut(s) 1397
BmiI GGNNCC 2 cut(s) 699, 1017
BmrFI CCNGG 7 cut(s) 153, 171, 920, 939, 957, 1071, 1485
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 1 cut(s) 469
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 1 cut(s) 1226
BpmI CTGGAG 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 804
BpuMI CCSGG 3 cut(s) 153, 171, 920
Bsa29I ATCGAT 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 1489
BsaI GGTCTC 1 cut(s) 1294
BsaJI CCNNGG 3 cut(s) 95, 1000, 1451
BsaWI WCCGGW 1 cut(s) 1438
BsaXI ACNNNNNCTCC 2 cut(s) 1051, 1081
Bsc4I CCNNNNNNNGG 1 cut(s) 1006
Bse1I ACTGG 3 cut(s) 281, 1016, 1225
Bse21I CCTNAGG 1 cut(s) 1080
Bse3DI GCAATG 1 cut(s) 1197
BseBI CCWGG 4 cut(s) 939, 957, 1071, 1485
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 3 cut(s) 95, 1000, 1451
BseGI GGATG 4 cut(s) 111, 864, 1427, 1431
BseLI CCNNNNNNNGG 1 cut(s) 1006
BseMI GCAATG 1 cut(s) 1197
BseMII CTCAG 7 cut(s) 421, 641, 839, 901, 1034, 1159, 1458
BseNI ACTGG 3 cut(s) 281, 1016, 1225
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 1 cut(s) 469
BseYI CCCAGC 1 cut(s) 1005
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 2 cut(s) 97, 1248
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 3 cut(s) 612, 1066, 1224
BshVI ATCGAT 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 143, 927
BsiSI CCGG 4 cut(s) 152, 171, 919, 1439
BsiWI CGTACG 2 cut(s) 20, 131
BslFI GGGAC 2 cut(s) 314, 1022
BslI CCNNNNNNNGG 1 cut(s) 1006
BsmAI GTCTC 4 cut(s) 723, 1269, 1294, 1496
BsmBI CGTCTC 1 cut(s) 1496
BsmFI GGGAC 2 cut(s) 314, 1022
BsnI GGCC 3 cut(s) 612, 1066, 1224
Bso31I GGTCTC 1 cut(s) 1294
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 2 cut(s) 143, 927
Bsp143I GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
Bsp68I TCGCGA 1 cut(s) 1248
BspACI CCGC 9 cut(s) 4, 41, 65, 79, 92, 95, 97, 258, 633
BspANI GGCC 3 cut(s) 612, 1066, 1224
BspCNI CTCAG 7 cut(s) 420, 640, 840, 900, 1033, 1158, 1457
BspDI ATCGAT 1 cut(s) 447
BspFNI CGCG 2 cut(s) 97, 1248
BspLI GGNNCC 2 cut(s) 699, 1017
BspPI GGATC 4 cut(s) 371, 1010, 1023, 1251
BspQI GCTCTTC 2 cut(s) 14, 223
BspT104I TTCGAA 1 cut(s) 804
BspTNI GGTCTC 1 cut(s) 1294
BsrDI GCAATG 1 cut(s) 1197
BsrI ACTGG 3 cut(s) 281, 1016, 1225
BssECI CCNNGG 3 cut(s) 95, 1000, 1451
BssMI GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
BssNI GRCGYC 1 cut(s) 1489
BssT1I CCWWGG 2 cut(s) 1000, 1451
Bst2UI CCWGG 4 cut(s) 939, 957, 1071, 1485
Bst4CI ACNGT 5 cut(s) 20, 128, 702, 1051, 1512
Bst6I CTCTTC 4 cut(s) 14, 51, 223, 777
BstACI GRCGYC 1 cut(s) 1489
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 1 cut(s) 756
BstDSI CCRYGG 1 cut(s) 95
BstEII GGTNACC 1 cut(s) 959
BstF5I GGATG 4 cut(s) 111, 864, 1427, 1431
BstFNI CGCG 2 cut(s) 97, 1248
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 7 cut(s) 194, 347, 379, 458, 1018, 1246, 1333
BstMAI GTCTC 4 cut(s) 723, 1269, 1294, 1496
BstMBI GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 2 cut(s) 64, 1254
BstNI CCWGG 4 cut(s) 939, 957, 1071, 1485
BstNSI RCATGY 3 cut(s) 718, 758, 1127
BstPI GGTNACC 1 cut(s) 959
BstSCI CCNGG 7 cut(s) 151, 169, 918, 937, 955, 1069, 1483
BstSFI CTRYAG 3 cut(s) 582, 986, 1299
BstUI CGCG 2 cut(s) 97, 1248
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 1 cut(s) 1226
BstX2I RGATCY 2 cut(s) 376, 1015
BstYI RGATCY 2 cut(s) 376, 1015
Bsu15I ATCGAT 1 cut(s) 447
Bsu36I CCTNAGG 1 cut(s) 1080
BsuRI GGCC 3 cut(s) 612, 1066, 1224
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 95
BtgZI GCGATG 1 cut(s) 1263
BtrI CACGTC 1 cut(s) 1381
BtsCI GGATG 4 cut(s) 111, 864, 1427, 1431
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 2 cut(s) 394, 895
BtuMI TCGCGA 1 cut(s) 1248
Cac8I GCNNGC 1 cut(s) 756
CfoI GCGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 1397
Cfr42I CCGCGG 1 cut(s) 98
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 6 cut(s) 21, 132, 656, 842, 1033, 1059
CviAII CATG 8 cut(s) 524, 608, 715, 755, 793, 965, 1124, 1271
CviQI GTAC 6 cut(s) 21, 132, 656, 842, 1033, 1059
DpnI GATC 7 cut(s) 193, 346, 378, 457, 1017, 1245, 1332
DpnII GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
EaeI YGGCCR 1 cut(s) 1222
Eam1104I CTCTTC 4 cut(s) 14, 51, 223, 777
EarI CTCTTC 4 cut(s) 14, 51, 223, 777
EciI GGCGGA 3 cut(s) 68, 81, 273
Ecl136II GAGCTC 2 cut(s) 141, 925
Eco130I CCWWGG 2 cut(s) 1000, 1451
Eco24I GRGCYC 2 cut(s) 143, 927
Eco31I GGTCTC 1 cut(s) 1294
Eco47I GGWCC 1 cut(s) 1397
Eco53kI GAGCTC 2 cut(s) 141, 925
Eco57I CTGAAG 3 cut(s) 165, 1182, 1489
Eco81I CCTNAGG 1 cut(s) 1080
Eco91I GGTNACC 1 cut(s) 959
EcoICRI GAGCTC 2 cut(s) 141, 925
EcoO109I RGGNCCY 1 cut(s) 1397
EcoO65I GGTNACC 1 cut(s) 959
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 4 cut(s) 937, 955, 1069, 1483
EcoT14I CCWWGG 2 cut(s) 1000, 1451
EcoT22I ATGCAT 2 cut(s) 716, 1199
EcoT38I GRGCYC 2 cut(s) 143, 927
ErhI CCWWGG 2 cut(s) 1000, 1451
Esp3I CGTCTC 1 cut(s) 1496
FaeI CATG 8 cut(s) 527, 611, 718, 758, 796, 968, 1127, 1274
FaqI GGGAC 2 cut(s) 314, 1022
FatI CATG 8 cut(s) 523, 607, 714, 754, 792, 964, 1123, 1270
FblI GTMKAC 1 cut(s) 985
Fnu4HI GCNGC 4 cut(s) 5, 65, 95, 483
FokI GGATG 4 cut(s) 118, 871, 1414, 1418
FriOI GRGCYC 2 cut(s) 143, 927
Fsp4HI GCNGC 4 cut(s) 5, 65, 95, 483
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 150
GluI GCNGC 4 cut(s) 5, 65, 95, 483
GsaI CCCAGC 1 cut(s) 1009
GsuI CTGGAG 1 cut(s) 960
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 3 cut(s) 612, 1066, 1224
HapII CCGG 4 cut(s) 152, 171, 919, 1439
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 1489
Hin1II CATG 8 cut(s) 527, 611, 718, 758, 796, 968, 1127, 1274
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 7 cut(s) 197, 444, 746, 1183, 1307, 1370, 1442
HpaII CCGG 4 cut(s) 152, 171, 919, 1439
HphI GGTGA 4 cut(s) 30, 874, 1316, 1388
Hpy166II GTNNAC 3 cut(s) 515, 893, 986
Hpy188I TCNGA 8 cut(s) 145, 196, 544, 723, 1023, 1148, 1335, 1508
Hpy188III TCNNGA 3 cut(s) 1187, 1241, 1247
Hpy8I GTNNAC 3 cut(s) 515, 893, 986
Hpy99I CGWCG 2 cut(s) 128, 139
HpyAV CCTTC 5 cut(s) 83, 1185, 1410, 1487, 1513
HpyCH4III ACNGT 5 cut(s) 20, 128, 702, 1051, 1512
HpyCH4IV ACGT 2 cut(s) 1380, 1489
HpyCH4V TGCA 6 cut(s) 371, 714, 737, 758, 1197, 1346
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 1254
HpySE526I ACGT 2 cut(s) 1380, 1489
Hsp92I GRCGYC 1 cut(s) 1489
Hsp92II CATG 8 cut(s) 527, 611, 718, 758, 796, 968, 1127, 1274
HspAI GCGC 1 cut(s) 149
KspI CCGCGG 1 cut(s) 98
Kzo9I GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
LguI GCTCTTC 2 cut(s) 14, 223
LmnI GCTCC 2 cut(s) 922, 971
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 1 cut(s) 469
MaeI CTAG 1 cut(s) 464
MaeII ACGT 2 cut(s) 1380, 1489
MaeIII GTNAC 6 cut(s) 163, 202, 880, 959, 1376, 1423
MalI GATC 7 cut(s) 193, 346, 378, 457, 1017, 1245, 1332
MboI GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
MboII GAAGA 9 cut(s) 38, 80, 121, 210, 213, 488, 794, 1175, 1226
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 2 cut(s) 376, 1015
MhlI GDGCHC 2 cut(s) 143, 927
MluCI AATT 8 cut(s) 31, 112, 178, 186, 306, 587, 800, 1085
MlyI GAGTC 2 cut(s) 1177, 1436
MmeI TCCRAC 2 cut(s) 274, 1443
Mph1103I ATGCAT 2 cut(s) 716, 1199
MroXI GAANNNNTTC 2 cut(s) 301, 875
MseI TTAA 5 cut(s) 452, 468, 932, 1134, 1516
MspA1I CMGCKG 2 cut(s) 97, 210
MspI CCGG 4 cut(s) 152, 171, 919, 1439
MspR9I CCNGG 7 cut(s) 153, 171, 920, 939, 957, 1071, 1485
MunI CAATTG 1 cut(s) 306
MvaI CCWGG 4 cut(s) 939, 957, 1071, 1485
MvnI CGCG 2 cut(s) 97, 1248
MwoI GCNNNNNNNGC 2 cut(s) 64, 1254
NciI CCSGG 3 cut(s) 153, 171, 920
NdeII GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
NlaIII CATG 8 cut(s) 527, 611, 718, 758, 796, 968, 1127, 1274
NlaIV GGNNCC 2 cut(s) 699, 1017
NmeAIII GCCGAG 1 cut(s) 1042
NmuCI GTSAC 5 cut(s) 163, 202, 880, 1376, 1423
NruI TCGCGA 1 cut(s) 1248
NsiI ATGCAT 2 cut(s) 716, 1199
NspI RCATGY 3 cut(s) 718, 758, 1127
NspV TTCGAA 1 cut(s) 804
PaeI GCATGC 1 cut(s) 758
PciSI GCTCTTC 2 cut(s) 14, 223
PcsI WCGNNNNNNNCGW 2 cut(s) 698, 1486
PdmI GAANNNNTTC 2 cut(s) 301, 875
PfeI GAWTC 5 cut(s) 197, 444, 746, 1307, 1370
Pfl23II CGTACG 2 cut(s) 20, 131
PflFI GACNNNGTC 1 cut(s) 1230
PfoI TCCNGGA 2 cut(s) 937, 1483
PkrI GCNGC 4 cut(s) 6, 66, 96, 484
PleI GAGTC 2 cut(s) 1177, 1436
PpsI GAGTC 2 cut(s) 1177, 1436
PpuMI RGGWCCY 1 cut(s) 1397
Psp124BI GAGCTC 2 cut(s) 143, 927
Psp5II RGGWCCY 1 cut(s) 1397
Psp6I CCWGG 4 cut(s) 937, 955, 1069, 1483
PspEI GGTNACC 1 cut(s) 959
PspFI CCCAGC 1 cut(s) 1005
PspGI CCWGG 4 cut(s) 937, 955, 1069, 1483
PspLI CGTACG 2 cut(s) 20, 131
PspN4I GGNNCC 2 cut(s) 699, 1017
PspPI GGNCC 1 cut(s) 1397
PspPPI RGGWCCY 1 cut(s) 1397
PsuI RGATCY 2 cut(s) 376, 1015
PsyI GACNNNGTC 1 cut(s) 1230
PvuII CAGCTG 1 cut(s) 210
RruI TCGCGA 1 cut(s) 1248
RsaI GTAC 6 cut(s) 22, 133, 657, 843, 1034, 1060
RsaNI GTAC 6 cut(s) 21, 132, 656, 842, 1033, 1059
SacI GAGCTC 2 cut(s) 143, 927
SacII CCGCGG 1 cut(s) 98
SapI GCTCTTC 2 cut(s) 14, 223
SaqAI TTAA 5 cut(s) 452, 468, 932, 1134, 1516
SatI GCNGC 4 cut(s) 5, 65, 95, 483
Sau3AI GATC 7 cut(s) 191, 344, 376, 455, 1015, 1243, 1330
Sau96I GGNCC 1 cut(s) 1397
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 2 cut(s) 1177, 1436
ScrFI CCNGG 7 cut(s) 153, 171, 920, 939, 957, 1071, 1485
SduI GDGCHC 2 cut(s) 143, 927
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 3 cut(s) 582, 986, 1299
Sfr303I CCGCGG 1 cut(s) 98
SfuI TTCGAA 1 cut(s) 804
SgrBI CCGCGG 1 cut(s) 98
SinI GGWCC 1 cut(s) 1397
SphI GCATGC 1 cut(s) 758
Sse9I AATT 8 cut(s) 31, 112, 178, 186, 306, 587, 800, 1085
SsiI CCGC 9 cut(s) 4, 41, 65, 79, 92, 95, 97, 258, 633
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 2 cut(s) 143, 927
StyD4I CCNGG 7 cut(s) 151, 169, 918, 937, 955, 1069, 1483
StyI CCWWGG 2 cut(s) 1000, 1451
TaaI ACNGT 5 cut(s) 20, 128, 702, 1051, 1512
TaiI ACGT 2 cut(s) 1383, 1492
TaqI TCGA 5 cut(s) 190, 442, 447, 804, 1403
TasI AATT 8 cut(s) 31, 112, 178, 186, 306, 587, 800, 1085
TatI WGTACW 1 cut(s) 841
TauI GCSGC 3 cut(s) 7, 67, 97
TfiI GAWTC 5 cut(s) 197, 444, 746, 1307, 1370
Tru1I TTAA 5 cut(s) 452, 468, 932, 1134, 1516
Tru9I TTAA 5 cut(s) 452, 468, 932, 1134, 1516
TscAI CASTG 2 cut(s) 401, 895
TseFI GTSAC 5 cut(s) 163, 202, 880, 1376, 1423
TseI GCWGC 1 cut(s) 482
Tsp45I GTSAC 5 cut(s) 163, 202, 880, 1376, 1423
TspGWI ACGGA 5 cut(s) 172, 190, 836, 1050, 1290
TspRI CASTG 2 cut(s) 401, 895
Tth111I GACNNNGTC 1 cut(s) 1230
VpaK11BI GGWCC 1 cut(s) 1397
XapI RAATTY 3 cut(s) 587, 800, 1085
XceI RCATGY 3 cut(s) 718, 758, 1127
XmiI GTMKAC 1 cut(s) 985
XmnI GAANNNNTTC 2 cut(s) 301, 875
XspI CTAG 1 cut(s) 464
ZraI GACGTC 1 cut(s) 1490
ZrmI AGTACT 1 cut(s) 843
Zsp2I ATGCAT 2 cut(s) 716, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.