Rmu_sc0002677.1_g000024
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002677.1
OG0007643
Physical Location & Seq
Forward (+)
118059 .. 120133
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002677.1_g000024.1.cds

Sequence Viewer

Length: 1392 bp
atgtggtggctcttgcaccgtacgaccctcctctccccaccccttcttcgtcgttcattccctccccgccaaattcgtccaccggaaaaaatgagtttcaggaacgacgggaactcacccgccactcccggtggcaattccgtcatttccttaagccccgtatcagctgctaatgttggaaacagagactgtcagattttaaaggttggaagtattcctattctgatttacgatagttttggtaatgtagttaagcggccaattgggtttggagttacatcagggtttagtgatgagaagcaatgtgcttcactaagtgatggtattcgtttcgatgaaatgagatcgaaagtggaggatgcttgtgtgagttcaaattcggagagtgcaagtgaaagggaacatgagaggtggagtccaatgccggattggaaacgccaattgcgcgaatgctctttgcctacggaatttgggatgaaaaggaagcaccacagtcgtgcagaaaagcggcattctgtaccgagaaatttgaagtatgcacctgttaccaagagttcgggtttatctaagcgtttgcattgttatgaaccgtttgatatatgcttggatgggagaaattctgctcttgaagcttcttttcatgcaagaatgatggatgcagaggacacggtggaatttgctaatccagaacggatggtggaatctaaagatccagtagtactgaggccaggaatggtcttattgaaaaattatgtcagcctcagtcaacaggttgaaattgtaaagacatgtcgggagctcggtttaggtcctggggggttttatcaaccagttttccagcatggaagaaaacttcatctgcagatgatgtgcctgggccagaattgggatcctgagactaggatatatgaagttatacgaccaatagatggaactcggcctcctggtattcctaatgaatttagtttgttggttgaaagagcaattcaagaggcacatgcccatctcaaagataaaatcagatgtagcagtgtagaaaaagtgctaccttcaatgactccagacatatgcatcgccaacttttatacaactagtgggaggcttggtcttcatcaggattgtgatgaaagcaaagagtctctccaagaagaattacctgtcgtctccatttctataggagattctgcagatttcctctatggggatcagagagatattgataaggcagagagtgttgttttgaattcaggagatgtgttaatattcggtggtcgttcaagacatatatttcatggtgtaacatccatcataccaaactcagctccaaacaatttgctaaaagaagctaggcttcgtcctggccgtctgaatcttaccttcagacagtattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

463

Amino Acids

52.05

Weight (kDa)

8.42

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 929
AccII CGCG 1 cut(s) 447
AciI CCGC 4 cut(s) 67, 120, 256, 508
AclWI GGATC 4 cut(s) 704, 884, 897, 1212
AcoI YGGCCR 2 cut(s) 257, 1360
AcsI RAATTY 8 cut(s) 72, 376, 467, 526, 616, 674, 959, 1243
AcuI CTGAAG 1 cut(s) 1363
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 3 cut(s) 22, 519, 720
AfiI CCNNNNNNNGG 3 cut(s) 886, 929, 1201
AflII CTTAAG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 788
AhlI ACTAGT 1 cut(s) 1091
AjnI CCWGG 5 cut(s) 727, 811, 873, 943, 1357
AleI CACNNNNGTG 1 cut(s) 495
AloI GAACNNNNNNTCC 2 cut(s) 925, 957
AluBI AGCT 5 cut(s) 167, 632, 799, 1322, 1346
AluI AGCT 5 cut(s) 167, 632, 799, 1322, 1346
Alw21I GWGCWC 1 cut(s) 801
Alw26I GTCTC 4 cut(s) 180, 890, 1143, 1168
AlwI GGATC 4 cut(s) 704, 884, 897, 1212
AlwNI CAGNNNCTG 1 cut(s) 189
AoxI GGCC 5 cut(s) 257, 725, 877, 938, 1360
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 8 cut(s) 72, 376, 467, 526, 616, 674, 959, 1243
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 809, 877
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 108
AvaII GGWCC 1 cut(s) 809
BamHI GGATCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 1100
Bbv12I GWGCWC 1 cut(s) 801
BbvI GCAGC 1 cut(s) 154
BccI CCATC 7 cut(s) 314, 602, 646, 688, 923, 1011, 1313
BceAI ACGGC 1 cut(s) 1347
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 5 cut(s) 729, 813, 875, 945, 1359
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 4 cut(s) 180, 890, 1143, 1168
BcuI ACTAGT 1 cut(s) 1091
BfaI CTAG 3 cut(s) 900, 1092, 1347
BfmI CTRYAG 3 cut(s) 860, 1173, 1185
BfrI CTTAAG 1 cut(s) 151
BisI GCNGC 3 cut(s) 168, 257, 509
BlsI GCNGC 3 cut(s) 169, 258, 510
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
Bme18I GGWCC 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 809, 877
BmiI GGNNCC 1 cut(s) 891
BmrFI CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
BmsI GCATC 3 cut(s) 349, 646, 1080
BpiI GAAGAC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 1044
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 812, 874
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 4 cut(s) 925, 955, 1090, 1120
Bsc4I CCNNNNNNNGG 3 cut(s) 886, 929, 1201
Bse1I ACTGG 2 cut(s) 713, 830
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
BseDI CCNNGG 2 cut(s) 812, 874
BseGI GGATG 6 cut(s) 364, 480, 613, 661, 699, 1301
BseLI CCNNNNNNNGG 3 cut(s) 886, 929, 1201
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 4 cut(s) 713, 775, 885, 1332
BseNI ACTGG 2 cut(s) 713, 830
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 5 cut(s) 259, 727, 879, 940, 1362
BsiHKAI GWGCWC 1 cut(s) 801
BsiSI CCGG 3 cut(s) 83, 129, 425
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 3 cut(s) 886, 929, 1201
BsmAI GTCTC 4 cut(s) 180, 890, 1143, 1168
BsmBI CGTCTC 1 cut(s) 1168
BsmI GAATGC 2 cut(s) 455, 511
BsnI GGCC 5 cut(s) 259, 727, 879, 940, 1362
Bsp1286I GDGCHC 1 cut(s) 801
Bsp143I GATC 4 cut(s) 344, 709, 889, 1204
BspACI CCGC 4 cut(s) 67, 120, 256, 508
BspANI GGCC 5 cut(s) 259, 727, 879, 940, 1362
BspCNI CTCAG 4 cut(s) 714, 774, 886, 1331
BspFNI CGCG 1 cut(s) 447
BspLI GGNNCC 1 cut(s) 891
BspMAI CTGCAG 2 cut(s) 864, 1189
BspPI GGATC 4 cut(s) 704, 884, 897, 1212
BspTI CTTAAG 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 308
BsrI ACTGG 2 cut(s) 713, 830
BssECI CCNNGG 2 cut(s) 812, 874
BssMI GATC 4 cut(s) 344, 709, 889, 1204
Bst2UI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
Bst4CI ACNGT 6 cut(s) 20, 191, 494, 591, 670, 1386
BstAFI CTTAAG 1 cut(s) 151
BstDEI CTNAG 6 cut(s) 314, 567, 722, 761, 894, 1318
BstF5I GGATG 6 cut(s) 364, 480, 613, 661, 699, 1301
BstFNI CGCG 1 cut(s) 447
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 4 cut(s) 347, 712, 892, 1207
BstMAI GTCTC 4 cut(s) 180, 890, 1143, 1168
BstMBI GATC 4 cut(s) 344, 709, 889, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 444, 629
BstNI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
BstNSI RCATGY 2 cut(s) 792, 1001
BstSCI CCNGG 6 cut(s) 127, 727, 811, 873, 943, 1357
BstSFI CTRYAG 3 cut(s) 860, 1173, 1185
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 1100
BstX2I RGATCY 2 cut(s) 709, 889
BstYI RGATCY 2 cut(s) 709, 889
BsuRI GGCC 5 cut(s) 259, 727, 879, 940, 1362
BtgZI GCGATG 1 cut(s) 1057
BtsCI GGATG 6 cut(s) 364, 480, 613, 661, 699, 1301
BtsI GCAGTG 1 cut(s) 1036
BtsIMutI CAGTG 1 cut(s) 1036
CaiI CAGNNNCTG 1 cut(s) 189
CfoI GCGC 1 cut(s) 447
Cfr13I GGNCC 2 cut(s) 809, 877
Csp6I GTAC 3 cut(s) 21, 518, 719
CviAII CATG 6 cut(s) 404, 641, 789, 842, 998, 1292
CviQI GTAC 3 cut(s) 21, 518, 719
DdeI CTNAG 6 cut(s) 314, 567, 722, 761, 894, 1318
DpnI GATC 4 cut(s) 346, 711, 891, 1206
DpnII GATC 4 cut(s) 344, 709, 889, 1204
DraI TTTAAA 1 cut(s) 201
DraIII CACNNNGTG 1 cut(s) 317
EaeI YGGCCR 2 cut(s) 257, 1360
Ecl136II GAGCTC 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 801
Eco47I GGWCC 1 cut(s) 809
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 1 cut(s) 1363
EcoICRI GAGCTC 1 cut(s) 799
EcoO109I RGGNCCY 1 cut(s) 809
EcoRI GAATTC 1 cut(s) 1243
EcoRII CCWGG 5 cut(s) 727, 811, 873, 943, 1357
EcoT22I ATGCAT 1 cut(s) 1073
EcoT38I GRGCYC 1 cut(s) 801
Esp3I CGTCTC 1 cut(s) 1168
FaeI CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
FalI AAGNNNNNCTT 2 cut(s) 1335, 1367
FatI CATG 6 cut(s) 403, 640, 788, 841, 997, 1291
FauI CCCGC 2 cut(s) 74, 127
FauNDI CATATG 1 cut(s) 1067
Fnu4HI GCNGC 3 cut(s) 168, 257, 509
FokI GGATG 6 cut(s) 371, 487, 620, 668, 706, 1288
FriOI GRGCYC 1 cut(s) 801
Fsp4HI GCNGC 3 cut(s) 168, 257, 509
FspBI CTAG 3 cut(s) 900, 1092, 1347
GlaI GCGC 1 cut(s) 446
GluI GCNGC 3 cut(s) 168, 257, 509
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 5 cut(s) 259, 727, 879, 940, 1362
HapII CCGG 3 cut(s) 83, 129, 425
HhaI GCGC 1 cut(s) 447
Hin1II CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HincII GTYRAC 1 cut(s) 767
HindII GTYRAC 1 cut(s) 767
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 6 cut(s) 415, 701, 1057, 1136, 1181, 1369
HpaII CCGG 3 cut(s) 83, 129, 425
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 80, 767
Hpy188I TCNGA 7 cut(s) 195, 225, 382, 1022, 1209, 1368, 1382
Hpy8I GTNNAC 2 cut(s) 80, 767
Hpy99I CGWCG 2 cut(s) 54, 110
HpyAV CCTTC 3 cut(s) 53, 1059, 1387
HpyCH4III ACNGT 6 cut(s) 20, 191, 494, 591, 670, 1386
HpyF10VI GCNNNNNNNGC 2 cut(s) 444, 629
HpyF3I CTNAG 6 cut(s) 314, 567, 722, 761, 894, 1318
Hsp92II CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
HspAI GCGC 1 cut(s) 445
Kzo9I GATC 4 cut(s) 344, 709, 889, 1204
LmnI GCTCC 2 cut(s) 796, 1327
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 3 cut(s) 349, 646, 1080
MaeI CTAG 3 cut(s) 900, 1092, 1347
MaeIII GTNAC 3 cut(s) 274, 544, 1297
MalI GATC 4 cut(s) 346, 711, 891, 1206
MboI GATC 4 cut(s) 344, 709, 889, 1204
MboII GAAGA 4 cut(s) 38, 858, 1100, 1160
MfeI CAATTG 2 cut(s) 261, 440
MflI RGATCY 2 cut(s) 709, 889
MhlI GDGCHC 1 cut(s) 801
MlyI GAGTC 3 cut(s) 424, 1051, 1145
MmeI TCCRAC 2 cut(s) 157, 187
Mph1103I ATGCAT 1 cut(s) 1073
MseI TTAA 4 cut(s) 152, 200, 252, 1259
MslI CAYNNNNRTG 2 cut(s) 495, 582
MspA1I CMGCKG 1 cut(s) 167
MspCI CTTAAG 1 cut(s) 151
MspI CCGG 3 cut(s) 83, 129, 425
MspR9I CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
MunI CAATTG 2 cut(s) 261, 440
Mva1269I GAATGC 2 cut(s) 455, 511
MvaI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 444, 629
NciI CCSGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 1067
NdeII GATC 4 cut(s) 344, 709, 889, 1204
NlaIII CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
NlaIV GGNNCC 1 cut(s) 891
NmeAIII GCCGAG 1 cut(s) 916
NsiI ATGCAT 1 cut(s) 1073
NspI RCATGY 2 cut(s) 792, 1001
OliI CACNNNNGTG 1 cut(s) 495
PciI ACATGT 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 1360
PctI GAATGC 2 cut(s) 455, 511
PfeI GAWTC 3 cut(s) 701, 1181, 1369
Pfl23II CGTACG 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 3 cut(s) 169, 258, 510
PleI GAGTC 3 cut(s) 423, 1051, 1144
PpsI GAGTC 3 cut(s) 423, 1051, 1144
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 5 cut(s) 727, 811, 873, 943, 1357
PspGI CCWGG 5 cut(s) 727, 811, 873, 943, 1357
PspLI CGTACG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 891
PspPI GGNCC 2 cut(s) 809, 877
PspPPI RGGWCCY 1 cut(s) 809
PstI CTGCAG 2 cut(s) 864, 1189
PstNI CAGNNNCTG 1 cut(s) 189
PsuI RGATCY 2 cut(s) 709, 889
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 3 cut(s) 22, 519, 720
RsaNI GTAC 3 cut(s) 21, 518, 719
RseI CAYNNNNRTG 2 cut(s) 495, 582
SacI GAGCTC 1 cut(s) 801
SaqAI TTAA 4 cut(s) 152, 200, 252, 1259
SatI GCNGC 3 cut(s) 168, 257, 509
Sau3AI GATC 4 cut(s) 344, 709, 889, 1204
Sau96I GGNCC 2 cut(s) 809, 877
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 3 cut(s) 424, 1051, 1145
ScrFI CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
SduI GDGCHC 1 cut(s) 801
SfaNI GCATC 3 cut(s) 349, 646, 1080
SfcI CTRYAG 3 cut(s) 860, 1173, 1185
SinI GGWCC 1 cut(s) 809
SmiMI CAYNNNNRTG 2 cut(s) 495, 582
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SpeI ACTAGT 1 cut(s) 1091
SsiI CCGC 4 cut(s) 67, 120, 256, 508
SspI AATATT 1 cut(s) 1263
SspMI CTAG 3 cut(s) 900, 1092, 1347
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 6 cut(s) 127, 727, 811, 873, 943, 1357
TaaI ACNGT 6 cut(s) 20, 191, 494, 591, 670, 1386
TaqI TCGA 2 cut(s) 333, 347
TatI WGTACW 1 cut(s) 718
TauI GCSGC 2 cut(s) 259, 511
TfiI GAWTC 3 cut(s) 701, 1181, 1369
Tru1I TTAA 4 cut(s) 152, 200, 252, 1259
Tru9I TTAA 4 cut(s) 152, 200, 252, 1259
TscAI CASTG 1 cut(s) 1036
TseI GCWGC 1 cut(s) 167
TspGWI ACGGA 3 cut(s) 130, 479, 706
TspRI CASTG 1 cut(s) 1036
Van91I CCANNNNNTGG 1 cut(s) 929
Vha464I CTTAAG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 8 cut(s) 72, 376, 467, 526, 616, 674, 959, 1243
XceI RCATGY 2 cut(s) 792, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XspI CTAG 3 cut(s) 900, 1092, 1347
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.