Rw3G009190
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007643
Physical Location & Seq
Reverse (-)
8764480 .. 8766942
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009190.1

Sequence Viewer

Length: 1053 bp
ATGAGATCGAAAGTGGAGGATGCTTGTGTGAGTTCAAATTCGGAGAGTGCAAGTGAAAGGGAACATGAGAGGTGGAGTCCAATGCCGGATTGGAAAAGCCAATTGCGCGAATGCTCTTTGCCTACGGAATTTGGGATGAAAAGGAAGCACCACAGTCGTGCAGAAAAGCGGCATTCTGTACCGAGAAATTTGAAGTATGCACCTGTTACCAAGAGTTCGGGTTTATCTAAGCGTTTGCATTGTTATGAACCGTTTGATATATGCTTGGATGGGAGAAATTCTGGTCTTGAAGCTTCTTTTCATGCAAGAATGATGGAAGCAGAGGACACGGTGGAATTCGCTAATCCAGAACGGATGGTGGAATCTAAAGATCCAGTAGTACTGAGGCCAGGAATGGTCTTATTGAAAAATTATGTCAGCCTCAGTCAACAGGTTGAAATTGTCAAGACATGTCGGGAGCTCGGTTTAGGTCCTGGGGGGTTTTATCAACCAGTTTTCCAGCATGGAAGAAAACTTCATCTGCAGATGATGTGCCTGGGCCAGAATTGGGATCCTGAGACTAGGATATATGAAGTTATACGACCAATAGATGGAACTCGGCCTCCTGGTATTCCTAATGAATTTAGTTTGTTGGTTGAAAGAGCAATTCAAGAGGCACATGCCCATCTCAAAGATAAAATCATATGTAGCAGTGTAGAAAAAGTGCTACCTTCAATGACTCCAGACATATGCATCGCCAACTTTTATACAACTAGTGGGAGGCTTGGTCTTCATCAGGATCGTGATGAAAGCGAAGAGTCTCTCCAAGAAGAATTACCTGTCGTCTCCATTTCTATAGGAGATTCTGCAGATTTCCTCTATGGGGATCAGAGAGATATTGATAAGGCAGAGAGTGTTGTTTTGAATTCAGGAGATGTGTTAATATTCGGTGGTCGTTCAAGACATATATTTCATGGTGTAACATCCATCATACCAAACTCAGCTCCAAACAATTTGCTAAAAGAAGCTAGGCTTCGTCCTGGCCGTCTGAATCTTACCTTCAGACAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

350

Amino Acids

39.63

Weight (kDa)

6.51

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 131 - 348 2.4e-46 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 590
AccII CGCG 1 cut(s) 108
AciI CCGC 1 cut(s) 169
AclWI GGATC 5 cut(s) 365, 545, 558, 786, 873
AcoI YGGCCR 1 cut(s) 1021
AcsI RAATTY 7 cut(s) 37, 128, 187, 277, 335, 620, 904
AcuI CTGAAG 1 cut(s) 1024
AfaI GTAC 2 cut(s) 180, 381
AfiI CCNNNNNNNGG 3 cut(s) 547, 590, 862
AflIII ACRYGT 1 cut(s) 449
AhlI ACTAGT 1 cut(s) 752
AjnI CCWGG 5 cut(s) 388, 472, 534, 604, 1018
AleI CACNNNNGTG 1 cut(s) 156
AloI GAACNNNNNNTCC 2 cut(s) 586, 618
AluBI AGCT 4 cut(s) 293, 460, 983, 1007
AluI AGCT 4 cut(s) 293, 460, 983, 1007
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 3 cut(s) 551, 804, 829
AlwI GGATC 5 cut(s) 365, 545, 558, 786, 873
AoxI GGCC 4 cut(s) 386, 538, 599, 1021
ApoI RAATTY 7 cut(s) 37, 128, 187, 277, 335, 620, 904
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 2 cut(s) 470, 538
AvaII GGWCC 1 cut(s) 470
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 462
BbsI GAAGAC 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 462
BccI CCATC 6 cut(s) 263, 307, 349, 584, 672, 974
BceAI ACGGC 1 cut(s) 1008
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 5 cut(s) 390, 474, 536, 606, 1020
BcoDI GTCTC 3 cut(s) 551, 804, 829
BcuI ACTAGT 1 cut(s) 752
BfaI CTAG 3 cut(s) 561, 753, 1008
BfmI CTRYAG 3 cut(s) 521, 834, 846
BisI GCNGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 171
BmcAI AGTACT 1 cut(s) 381
Bme1390I CCNGG 5 cut(s) 390, 474, 536, 606, 1020
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 470, 538
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 5 cut(s) 390, 474, 536, 606, 1020
BmsI GCATC 2 cut(s) 10, 741
BpiI GAAGAC 1 cut(s) 761
BpmI CTGGAG 1 cut(s) 705
BsaJI CCNNGG 2 cut(s) 473, 535
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 751, 781
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 590, 862
Bse1I ACTGG 2 cut(s) 374, 491
BseBI CCWGG 5 cut(s) 390, 474, 536, 606, 1020
BseDI CCNNGG 2 cut(s) 473, 535
BseGI GGATG 5 cut(s) 25, 141, 274, 360, 962
BseLI CCNNNNNNNGG 3 cut(s) 547, 590, 862
BseMII CTCAG 4 cut(s) 374, 436, 546, 993
BseNI ACTGG 2 cut(s) 374, 491
BsgI GTGCAG 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 4 cut(s) 388, 540, 601, 1023
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 1 cut(s) 86
BslI CCNNNNNNNGG 3 cut(s) 547, 590, 862
BsmAI GTCTC 3 cut(s) 551, 804, 829
BsmBI CGTCTC 1 cut(s) 829
BsmI GAATGC 2 cut(s) 116, 172
BsnI GGCC 4 cut(s) 388, 540, 601, 1023
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 5 cut(s) 5, 370, 550, 778, 865
BspACI CCGC 1 cut(s) 169
BspANI GGCC 4 cut(s) 388, 540, 601, 1023
BspCNI CTCAG 4 cut(s) 375, 435, 547, 992
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 552
BspMAI CTGCAG 2 cut(s) 525, 850
BspPI GGATC 5 cut(s) 365, 545, 558, 786, 873
BsrI ACTGG 2 cut(s) 374, 491
BssECI CCNNGG 2 cut(s) 473, 535
BssMI GATC 5 cut(s) 5, 370, 550, 778, 865
Bst2UI CCWGG 5 cut(s) 390, 474, 536, 606, 1020
Bst4CI ACNGT 4 cut(s) 155, 252, 331, 1047
Bst6I CTCTTC 1 cut(s) 789
BstDEI CTNAG 5 cut(s) 228, 383, 422, 555, 979
BstF5I GGATG 5 cut(s) 25, 141, 274, 360, 962
BstFNI CGCG 1 cut(s) 108
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 5 cut(s) 8, 373, 553, 781, 868
BstMAI GTCTC 3 cut(s) 551, 804, 829
BstMBI GATC 5 cut(s) 5, 370, 550, 778, 865
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstNI CCWGG 5 cut(s) 390, 474, 536, 606, 1020
BstNSI RCATGY 2 cut(s) 453, 662
BstSCI CCNGG 5 cut(s) 388, 472, 534, 604, 1018
BstSFI CTRYAG 3 cut(s) 521, 834, 846
BstUI CGCG 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 761
BstX2I RGATCY 2 cut(s) 370, 550
BstYI RGATCY 2 cut(s) 370, 550
BsuRI GGCC 4 cut(s) 388, 540, 601, 1023
BtgZI GCGATG 1 cut(s) 718
BtsCI GGATG 5 cut(s) 25, 141, 274, 360, 962
BtsI GCAGTG 1 cut(s) 697
BtsIMutI CAGTG 1 cut(s) 697
CfoI GCGC 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 470, 538
Csp6I GTAC 2 cut(s) 179, 380
CviAII CATG 6 cut(s) 65, 302, 450, 503, 659, 953
CviQI GTAC 2 cut(s) 179, 380
DdeI CTNAG 5 cut(s) 228, 383, 422, 555, 979
DpnI GATC 5 cut(s) 7, 372, 552, 780, 867
DpnII GATC 5 cut(s) 5, 370, 550, 778, 865
EaeI YGGCCR 1 cut(s) 1021
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Ecl136II GAGCTC 1 cut(s) 460
Eco24I GRGCYC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 1024
EcoICRI GAGCTC 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 2 cut(s) 335, 904
EcoRII CCWGG 5 cut(s) 388, 472, 534, 604, 1018
EcoT22I ATGCAT 1 cut(s) 734
EcoT38I GRGCYC 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 829
FaeI CATG 6 cut(s) 68, 305, 453, 506, 662, 956
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FatI CATG 6 cut(s) 64, 301, 449, 502, 658, 952
FauNDI CATATG 2 cut(s) 683, 728
Fnu4HI GCNGC 1 cut(s) 170
FokI GGATG 5 cut(s) 32, 148, 281, 367, 949
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 1 cut(s) 170
FspBI CTAG 3 cut(s) 561, 753, 1008
GlaI GCGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 170
GsuI CTGGAG 1 cut(s) 705
HaeIII GGCC 4 cut(s) 388, 540, 601, 1023
HapII CCGG 1 cut(s) 86
HhaI GCGC 1 cut(s) 108
Hin1II CATG 6 cut(s) 68, 305, 453, 506, 662, 956
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 428
HindII GTYRAC 1 cut(s) 428
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 6 cut(s) 76, 362, 718, 797, 842, 1030
HpaII CCGG 1 cut(s) 86
Hpy166II GTNNAC 1 cut(s) 428
Hpy188I TCNGA 4 cut(s) 43, 870, 1029, 1043
Hpy8I GTNNAC 1 cut(s) 428
HpyAV CCTTC 2 cut(s) 720, 1048
HpyCH4III ACNGT 4 cut(s) 155, 252, 331, 1047
HpyCH4V TGCA 8 cut(s) 50, 161, 200, 238, 305, 523, 732, 848
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 5 cut(s) 228, 383, 422, 555, 979
Hsp92II CATG 6 cut(s) 68, 305, 453, 506, 662, 956
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 5 cut(s) 5, 370, 550, 778, 865
LmnI GCTCC 2 cut(s) 457, 988
LweI GCATC 2 cut(s) 10, 741
MaeI CTAG 3 cut(s) 561, 753, 1008
MaeIII GTNAC 2 cut(s) 205, 958
MalI GATC 5 cut(s) 7, 372, 552, 780, 867
MboI GATC 5 cut(s) 5, 370, 550, 778, 865
MboII GAAGA 4 cut(s) 519, 761, 806, 821
MfeI CAATTG 1 cut(s) 101
MflI RGATCY 2 cut(s) 370, 550
MhlI GDGCHC 1 cut(s) 462
MlyI GAGTC 3 cut(s) 85, 712, 806
MnlI CCTC 9 cut(s) 10, 63, 316, 378, 431, 612, 646, 753, 866
Mph1103I ATGCAT 1 cut(s) 734
MseI TTAA 1 cut(s) 920
MslI CAYNNNNRTG 2 cut(s) 156, 243
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 5 cut(s) 390, 474, 536, 606, 1020
MunI CAATTG 1 cut(s) 101
Mva1269I GAATGC 2 cut(s) 116, 172
MvaI CCWGG 5 cut(s) 390, 474, 536, 606, 1020
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 105
NdeI CATATG 2 cut(s) 683, 728
NdeII GATC 5 cut(s) 5, 370, 550, 778, 865
NlaIII CATG 6 cut(s) 68, 305, 453, 506, 662, 956
NlaIV GGNNCC 1 cut(s) 552
NmeAIII GCCGAG 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 734
NspI RCATGY 2 cut(s) 453, 662
OliI CACNNNNGTG 1 cut(s) 156
PciI ACATGT 1 cut(s) 449
PcsI WCGNNNNNNNCGW 1 cut(s) 1021
PctI GAATGC 2 cut(s) 116, 172
PfeI GAWTC 3 cut(s) 362, 842, 1030
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 1 cut(s) 171
PleI GAGTC 3 cut(s) 84, 712, 805
PpsI GAGTC 3 cut(s) 84, 712, 805
PpuMI RGGWCCY 1 cut(s) 470
PscI ACATGT 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 5 cut(s) 388, 472, 534, 604, 1018
PspGI CCWGG 5 cut(s) 388, 472, 534, 604, 1018
PspN4I GGNNCC 1 cut(s) 552
PspPI GGNCC 2 cut(s) 470, 538
PspPPI RGGWCCY 1 cut(s) 470
PstI CTGCAG 2 cut(s) 525, 850
PsuI RGATCY 2 cut(s) 370, 550
RsaI GTAC 2 cut(s) 180, 381
RsaNI GTAC 2 cut(s) 179, 380
RseI CAYNNNNRTG 2 cut(s) 156, 243
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 1 cut(s) 920
SatI GCNGC 1 cut(s) 170
Sau3AI GATC 5 cut(s) 5, 370, 550, 778, 865
Sau96I GGNCC 2 cut(s) 470, 538
ScaI AGTACT 1 cut(s) 381
SchI GAGTC 3 cut(s) 85, 712, 806
ScrFI CCNGG 5 cut(s) 390, 474, 536, 606, 1020
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 2 cut(s) 10, 741
SfcI CTRYAG 3 cut(s) 521, 834, 846
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 156, 243
SpeI ACTAGT 1 cut(s) 752
SsiI CCGC 1 cut(s) 169
SspI AATATT 1 cut(s) 924
SspMI CTAG 3 cut(s) 561, 753, 1008
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 5 cut(s) 388, 472, 534, 604, 1018
TaaI ACNGT 4 cut(s) 155, 252, 331, 1047
TaqI TCGA 1 cut(s) 8
TatI WGTACW 1 cut(s) 379
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 3 cut(s) 362, 842, 1030
Tru1I TTAA 1 cut(s) 920
Tru9I TTAA 1 cut(s) 920
TscAI CASTG 1 cut(s) 697
TspDTI ATGAA 9 cut(s) 152, 261, 290, 506, 585, 633, 761, 801, 941
TspGWI ACGGA 2 cut(s) 140, 367
TspRI CASTG 1 cut(s) 697
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 1 cut(s) 470
XapI RAATTY 7 cut(s) 37, 128, 187, 277, 335, 620, 904
XceI RCATGY 2 cut(s) 453, 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 87
XspI CTAG 3 cut(s) 561, 753, 1008
ZrmI AGTACT 1 cut(s) 381
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.