Rroxscaffold_6G00418670
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007643
Physical Location & Seq
Forward (+)
40252164 .. 40255002
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418670.1

Sequence Viewer

Length: 1407 bp
ATGTGGTGGCTCTTGCGCCGTACGACCCTTCTCTCCCCACCCTTTCTTCGTCGTTCATTCCCTCCCCGCCAAATTCGTCCACCGGAAAAAATGAGTTTCAGGAACTCACCCGCCACTCCCGGTGGCAATTCCGTCATTTCCTTAAGCCCCGTATCAGCAGCTAATGTTGGAAACAGAGATTGTCAGATTTTAAAGGTTGGAAGTATTCCTATTCTGATTTACGATAGTTGTGGTAATGTAGTTAAGCGGCCAATTGGGTTTGGAGTTGCATCAGGGTTTAGTGATAAGAAGCAATGTTCTTCAGTAAGTGATGGTATTTGTTTCGATGAAATGAGAACGAAAGTGGAGTTAGTGTCGGGTGATGATGTGGAGGCTGCTTGTATGAGTTCAAATTCGGAGAGTGTAAGTGAAAGGGAACATGAGAGGTGGAGTCCAATGCCGGATTGGAAACGCCAATTGCGTGAATGCTCTTTGCCTACGGAATTTGGGATGAAAAGGAAGCACCATCATCGTGCGGAAAAGCGGCATTCTGTACCGAGAAATTTGAAATATGCACCTGTTACCAAGAGTTCGGGTTTATCTAAGCGTTTGCATCGTTATGAACCGTTTGATATATGCTTGGATGGGAGAAATTCTGCTCTTGAAGCTTCTTTTCATGCAAGAATGATGGAAGCAGAGGACACGGTGGAATCTGCTAATCCAGAACGGATGGTGGAATTTAAAGATCCAATAATACTAAGGCCAGGGATGGTCTTGTTGAAAAATTATGTCAGCCTCTGTGAACAGGTTGAAATTGTAAAGATATGTCGGGAGCTCGGTTTAGGTCCTGGGGGGTTTTATCAACCAGTTTTCCAGCATGGAAGAAAACTTCATCTGCAGATGATGTGCCTGGGCCTGAATTGGGATCCTGAGACCAGGATATATGAAGGTATACGACCAATAGATGGCACTCGGCCTCCTGGTATTCCTAATGAATTTAGTTTGTTGGTTGAAAGAGCAATTCAAGAGGCACATGCCCATCTCAAAGATAAAATCAGATGTAGCAGTGTAGAAAAAGTGCTACCTTCAATGACTCCAGACATATGCATCGCCAACTTTTATACAACTAGTGGGAGGCTTGGTCTTCATCAGGATCATGATGAAAGCAAAGAGTCTCTCCAAGAAGGATTACCTGTCGTCTCCATTTCTATAGGAGATTCTGCAGATTTCCTCTATGGGGATCAGAGAGATATTGATAAGGCAGAGAGTGTTGTTTTGGATTCAGGAGATGTGTTAATATTCGGTGGTCGTTCAAGACATATATTTCATGGTGTAACATCCATCATACCAAACTCAGCTCCAAACAATTTGCTAAAAGAAACTAGGCTTCGTCCTGGCCGTCTGAATCTTACCTTCAGACAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

468

Amino Acids

52.65

Weight (kDa)

8.53

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 249 - 466 6.5e-45 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 944
AccI GTMKAC 1 cut(s) 931
AciI CCGC 5 cut(s) 67, 111, 247, 515, 523
AclWI GGATC 5 cut(s) 719, 899, 912, 1140, 1227
AcoI YGGCCR 2 cut(s) 248, 1375
AcsI RAATTY 7 cut(s) 72, 391, 482, 541, 631, 716, 974
AcuI CTGAAG 2 cut(s) 285, 1378
AfaI GTAC 2 cut(s) 22, 534
AfiI CCNNNNNNNGG 3 cut(s) 901, 944, 1216
AflII CTTAAG 1 cut(s) 142
AgsI TTSAA 9 cut(s) 390, 547, 644, 760, 791, 992, 1004, 1068, 1293
AhlI ACTAGT 1 cut(s) 1106
AjnI CCWGG 6 cut(s) 742, 826, 888, 914, 958, 1372
AluBI AGCT 4 cut(s) 161, 647, 814, 1337
AluI AGCT 4 cut(s) 161, 647, 814, 1337
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 3 cut(s) 905, 1158, 1183
AlwI GGATC 5 cut(s) 719, 899, 912, 1140, 1227
AlwNI CAGNNNCTG 1 cut(s) 777
AoxI GGCC 5 cut(s) 248, 740, 892, 953, 1375
ApeKI GCWGC 2 cut(s) 158, 374
ApoI RAATTY 7 cut(s) 72, 391, 482, 541, 631, 716, 974
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 824, 892
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 99, 371
AvaII GGWCC 1 cut(s) 824
BamHI GGATCC 1 cut(s) 904
BanII GRGCYC 1 cut(s) 816
BbsI GAAGAC 1 cut(s) 1115
Bbv12I GWGCWC 1 cut(s) 816
BbvI GCAGC 2 cut(s) 170, 361
BccI CCATC 9 cut(s) 305, 513, 617, 661, 703, 742, 938, 1026, 1328
BceAI ACGGC 2 cut(s) 3, 1362
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 6 cut(s) 744, 828, 890, 916, 960, 1374
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 3 cut(s) 905, 1158, 1183
BcuI ACTAGT 1 cut(s) 1106
BfaI CTAG 2 cut(s) 1107, 1362
BfmI CTRYAG 3 cut(s) 875, 1188, 1200
BfrI CTTAAG 1 cut(s) 142
BisI GCNGC 4 cut(s) 159, 248, 375, 524
BlsI GCNGC 4 cut(s) 160, 249, 376, 525
Bme1390I CCNGG 7 cut(s) 120, 744, 828, 890, 916, 960, 1374
Bme18I GGWCC 1 cut(s) 824
BmgT120I GGNCC 2 cut(s) 824, 892
BmiI GGNNCC 1 cut(s) 906
BmrFI CCNGG 7 cut(s) 120, 744, 828, 890, 916, 960, 1374
BmsI GCATC 3 cut(s) 278, 601, 1095
BpiI GAAGAC 1 cut(s) 1115
BpmI CTGGAG 1 cut(s) 1059
BpuMI CCSGG 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 3 cut(s) 743, 827, 889
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 6 cut(s) 338, 368, 940, 970, 1105, 1135
Bsc4I CCNNNNNNNGG 3 cut(s) 901, 944, 1216
Bse1I ACTGG 1 cut(s) 845
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 6 cut(s) 744, 828, 890, 916, 960, 1374
BseDI CCNNGG 3 cut(s) 743, 827, 889
BseGI GGATG 5 cut(s) 495, 628, 714, 753, 1316
BseLI CCNNNNNNNGG 3 cut(s) 901, 944, 1216
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 2 cut(s) 900, 1347
BseNI ACTGG 1 cut(s) 845
BseXI GCAGC 2 cut(s) 170, 361
BshFI GGCC 5 cut(s) 250, 742, 894, 955, 1377
BsiHKAI GWGCWC 1 cut(s) 816
BsiSI CCGG 3 cut(s) 83, 120, 440
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 3 cut(s) 901, 944, 1216
BsmAI GTCTC 3 cut(s) 905, 1158, 1183
BsmBI CGTCTC 1 cut(s) 1183
BsmI GAATGC 2 cut(s) 470, 526
BsnI GGCC 5 cut(s) 250, 742, 894, 955, 1377
Bso31I GGTCTC 1 cut(s) 905
Bsp1286I GDGCHC 1 cut(s) 816
Bsp143I GATC 4 cut(s) 724, 904, 1132, 1219
BspACI CCGC 5 cut(s) 67, 111, 247, 515, 523
BspANI GGCC 5 cut(s) 250, 742, 894, 955, 1377
BspCNI CTCAG 2 cut(s) 901, 1346
BspHI TCATGA 1 cut(s) 1135
BspLI GGNNCC 1 cut(s) 906
BspMAI CTGCAG 2 cut(s) 879, 1204
BspPI GGATC 5 cut(s) 719, 899, 912, 1140, 1227
BspTI CTTAAG 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 905
BsrDI GCAATG 1 cut(s) 299
BsrI ACTGG 1 cut(s) 845
BssECI CCNNGG 3 cut(s) 743, 827, 889
BssMI GATC 4 cut(s) 724, 904, 1132, 1219
BssNAI GTATAC 1 cut(s) 932
Bst1107I GTATAC 1 cut(s) 932
Bst2UI CCWGG 6 cut(s) 744, 828, 890, 916, 960, 1374
Bst4CI ACNGT 3 cut(s) 606, 685, 1401
BstAFI CTTAAG 1 cut(s) 142
BstDEI CTNAG 4 cut(s) 582, 737, 909, 1333
BstF5I GGATG 5 cut(s) 495, 628, 714, 753, 1316
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 4 cut(s) 727, 907, 1135, 1222
BstMAI GTCTC 3 cut(s) 905, 1158, 1183
BstMBI GATC 4 cut(s) 724, 904, 1132, 1219
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNI CCWGG 6 cut(s) 744, 828, 890, 916, 960, 1374
BstNSI RCATGY 1 cut(s) 1016
BstSCI CCNGG 7 cut(s) 118, 742, 826, 888, 914, 958, 1372
BstSFI CTRYAG 3 cut(s) 875, 1188, 1200
BstV1I GCAGC 2 cut(s) 170, 361
BstV2I GAAGAC 1 cut(s) 1115
BstX2I RGATCY 2 cut(s) 724, 904
BstYI RGATCY 2 cut(s) 724, 904
BstZ17I GTATAC 1 cut(s) 932
BsuRI GGCC 5 cut(s) 250, 742, 894, 955, 1377
BtgZI GCGATG 1 cut(s) 1072
BtsCI GGATG 5 cut(s) 495, 628, 714, 753, 1316
BtsI GCAGTG 1 cut(s) 1051
BtsIMutI CAGTG 1 cut(s) 1051
CaiI CAGNNNCTG 1 cut(s) 777
CciI TCATGA 1 cut(s) 1135
CfoI GCGC 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 824, 892
Csp6I GTAC 2 cut(s) 21, 533
CviAII CATG 6 cut(s) 419, 656, 857, 1013, 1136, 1307
CviQI GTAC 2 cut(s) 21, 533
DdeI CTNAG 4 cut(s) 582, 737, 909, 1333
DpnI GATC 4 cut(s) 726, 906, 1134, 1221
DpnII GATC 4 cut(s) 724, 904, 1132, 1219
DraI TTTAAA 2 cut(s) 192, 721
EaeI YGGCCR 2 cut(s) 248, 1375
Ecl136II GAGCTC 1 cut(s) 814
Eco24I GRGCYC 1 cut(s) 816
Eco31I GGTCTC 1 cut(s) 905
Eco47I GGWCC 1 cut(s) 824
Eco53kI GAGCTC 1 cut(s) 814
Eco57I CTGAAG 2 cut(s) 285, 1378
EcoICRI GAGCTC 1 cut(s) 814
EcoO109I RGGNCCY 1 cut(s) 824
EcoRII CCWGG 6 cut(s) 742, 826, 888, 914, 958, 1372
EcoT22I ATGCAT 1 cut(s) 1088
EcoT38I GRGCYC 1 cut(s) 816
Esp3I CGTCTC 1 cut(s) 1183
FaeI CATG 6 cut(s) 422, 659, 860, 1016, 1139, 1310
FatI CATG 6 cut(s) 418, 655, 856, 1012, 1135, 1306
FauI CCCGC 2 cut(s) 74, 118
FauNDI CATATG 1 cut(s) 1082
FblI GTMKAC 1 cut(s) 931
Fnu4HI GCNGC 4 cut(s) 159, 248, 375, 524
FokI GGATG 5 cut(s) 502, 635, 721, 760, 1303
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 4 cut(s) 159, 248, 375, 524
FspBI CTAG 2 cut(s) 1107, 1362
GlaI GCGC 1 cut(s) 17
GluI GCNGC 4 cut(s) 159, 248, 375, 524
GsuI CTGGAG 1 cut(s) 1059
HaeIII GGCC 5 cut(s) 250, 742, 894, 955, 1377
HapII CCGG 3 cut(s) 83, 120, 440
HhaI GCGC 1 cut(s) 18
Hin1II CATG 6 cut(s) 422, 659, 860, 1016, 1139, 1310
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 7 cut(s) 430, 689, 1072, 1151, 1196, 1259, 1384
HpaII CCGG 3 cut(s) 83, 120, 440
HphI GGTGA 2 cut(s) 99, 371
Hpy166II GTNNAC 3 cut(s) 80, 782, 932
Hpy188I TCNGA 7 cut(s) 186, 216, 397, 1037, 1224, 1383, 1397
Hpy8I GTNNAC 3 cut(s) 80, 782, 932
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 5 cut(s) 38, 920, 1074, 1157, 1402
HpyCH4III ACNGT 3 cut(s) 606, 685, 1401
HpyCH4V TGCA 7 cut(s) 269, 554, 592, 659, 877, 1086, 1202
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 4 cut(s) 582, 737, 909, 1333
Hsp92II CATG 6 cut(s) 422, 659, 860, 1016, 1139, 1310
HspAI GCGC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 724, 904, 1132, 1219
LmnI GCTCC 2 cut(s) 811, 1342
Lsp1109I GCAGC 2 cut(s) 170, 361
LweI GCATC 3 cut(s) 278, 601, 1095
MaeI CTAG 2 cut(s) 1107, 1362
MaeIII GTNAC 2 cut(s) 559, 1312
MalI GATC 4 cut(s) 726, 906, 1134, 1221
MboI GATC 4 cut(s) 724, 904, 1132, 1219
MboII GAAGA 4 cut(s) 38, 291, 873, 1115
MfeI CAATTG 2 cut(s) 252, 455
MflI RGATCY 2 cut(s) 724, 904
MhlI GDGCHC 1 cut(s) 816
MlyI GAGTC 3 cut(s) 439, 1066, 1160
MmeI TCCRAC 2 cut(s) 148, 178
MnlI CCTC 9 cut(s) 72, 364, 417, 670, 785, 966, 1000, 1107, 1220
Mph1103I ATGCAT 1 cut(s) 1088
MseI TTAA 5 cut(s) 143, 191, 243, 720, 1274
MslI CAYNNNNRTG 2 cut(s) 510, 597
MspCI CTTAAG 1 cut(s) 142
MspI CCGG 3 cut(s) 83, 120, 440
MspR9I CCNGG 7 cut(s) 120, 744, 828, 890, 916, 960, 1374
MunI CAATTG 2 cut(s) 252, 455
Mva1269I GAATGC 2 cut(s) 470, 526
MvaI CCWGG 6 cut(s) 744, 828, 890, 916, 960, 1374
MwoI GCNNNNNNNGC 1 cut(s) 644
NciI CCSGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 1082
NdeII GATC 4 cut(s) 724, 904, 1132, 1219
NlaIII CATG 6 cut(s) 422, 659, 860, 1016, 1139, 1310
NlaIV GGNNCC 1 cut(s) 906
NmeAIII GCCGAG 1 cut(s) 931
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 1 cut(s) 1016
PagI TCATGA 1 cut(s) 1135
PcsI WCGNNNNNNNCGW 2 cut(s) 457, 1375
PctI GAATGC 2 cut(s) 470, 526
PfeI GAWTC 4 cut(s) 689, 1196, 1259, 1384
Pfl23II CGTACG 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 944
PkrI GCNGC 4 cut(s) 160, 249, 376, 525
PleI GAGTC 3 cut(s) 438, 1066, 1159
PpsI GAGTC 3 cut(s) 438, 1066, 1159
PpuMI RGGWCCY 1 cut(s) 824
Psp124BI GAGCTC 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 6 cut(s) 742, 826, 888, 914, 958, 1372
PspGI CCWGG 6 cut(s) 742, 826, 888, 914, 958, 1372
PspLI CGTACG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 906
PspPI GGNCC 2 cut(s) 824, 892
PspPPI RGGWCCY 1 cut(s) 824
PstI CTGCAG 2 cut(s) 879, 1204
PstNI CAGNNNCTG 1 cut(s) 777
PsuI RGATCY 2 cut(s) 724, 904
RsaI GTAC 2 cut(s) 22, 534
RsaNI GTAC 2 cut(s) 21, 533
RseI CAYNNNNRTG 2 cut(s) 510, 597
SacI GAGCTC 1 cut(s) 816
SaqAI TTAA 5 cut(s) 143, 191, 243, 720, 1274
SatI GCNGC 4 cut(s) 159, 248, 375, 524
Sau3AI GATC 4 cut(s) 724, 904, 1132, 1219
Sau96I GGNCC 2 cut(s) 824, 892
SchI GAGTC 3 cut(s) 439, 1066, 1160
ScrFI CCNGG 7 cut(s) 120, 744, 828, 890, 916, 960, 1374
SduI GDGCHC 1 cut(s) 816
SfaNI GCATC 3 cut(s) 278, 601, 1095
SfcI CTRYAG 3 cut(s) 875, 1188, 1200
SinI GGWCC 1 cut(s) 824
SmiMI CAYNNNNRTG 2 cut(s) 510, 597
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
SpeI ACTAGT 1 cut(s) 1106
SsiI CCGC 5 cut(s) 67, 111, 247, 515, 523
SspI AATATT 1 cut(s) 1278
SspMI CTAG 2 cut(s) 1107, 1362
SstI GAGCTC 1 cut(s) 816
StyD4I CCNGG 7 cut(s) 118, 742, 826, 888, 914, 958, 1372
TaaI ACNGT 3 cut(s) 606, 685, 1401
TaqI TCGA 1 cut(s) 324
TauI GCSGC 2 cut(s) 250, 526
TfiI GAWTC 4 cut(s) 689, 1196, 1259, 1384
Tru1I TTAA 5 cut(s) 143, 191, 243, 720, 1274
Tru9I TTAA 5 cut(s) 143, 191, 243, 720, 1274
TscAI CASTG 1 cut(s) 1051
TseI GCWGC 2 cut(s) 158, 374
TspGWI ACGGA 3 cut(s) 121, 494, 721
TspRI CASTG 1 cut(s) 1051
Van91I CCANNNNNTGG 1 cut(s) 944
Vha464I CTTAAG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 7 cut(s) 72, 391, 482, 541, 631, 716, 974
XceI RCATGY 1 cut(s) 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 931
XspI CTAG 2 cut(s) 1107, 1362
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.