RLG00000024938
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007643
Physical Location & Seq
Forward (+)
40513960 .. 40516043
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024938

Sequence Viewer

Length: 1392 bp
ATGTGGTGGCTCTTGCGCCGTACGACCCTTCTCTCCCCAGCCTTTCTTCGTCGTTCATTCCCTCCCCGCCAAATTCGTCCACCGGAAAAAATGAGTTTCAGGAACGACGGGAACTCACCCGCCACTCCCGGTGGCAATTCCGTCATTTGCTTAAGCCCCGTTGGAAACAGAGATTGTCAGATTTTAAAGGTTGGAAGTATTCCTATTCTGAGTTACGATAGTTCTGGTAATAATGTAGTTATGCCAATTGGGTTTGCAGTTACATCAGGGTTTAGTGATAAGAAGCAAACAAGTGATGGTATTTGTTTCGATGAAATGAGAACGAAAGTGGAGTTAGTGTCGGGGGATGATGTGGAGGATGCTTGTGTGAGTTCAAAATCGAAGAGTGTGAGTGAAAGGGAACATGAGAGGTGGAGTCCAATGGCTGATTGGAAAAGCCAATTGCGTGAATGCTCTTTGCCTACGGAATTTGGGATGAAAAGGAAGCACCATCATCATGAGGAAAAGCGGCATTCTGTACCGAGAAATTTGAAATATGCACCTGTTACCGAGAGTTCGGGTTTATCTAAGCGTTTGCATCGTTATGAACCGTTTGATATATGCTTGGATGGGAGAAATTCTGCTCTTCAAGCTTCTTTTCATGCAAGAATGATGGAAGCAGAGGACACGGTGGAATTTGCTAATCCAGAACGGATGGTGGAATATAAAGATCCAGTAGTACTGAGGCCAGGAATGGTCTTATTGAAAAATTATGTCAGCCTCAGTGACCAGGTTGAAATTGTAAAGACATGTCGGGAGCTAGGTTTAGGTCCTGGGGGGTTTTATCAACCAGTTTTCCAGCATGGAAGAAAGCTTCATCTGCAGATGATGTGCCTGGGCCTGAATTGGGATCCTGAGACTAGGATATATGAAGATATACGACCAATAGATGGCACTCGTCCTCCTGGTATTCCTAATGAATTTAGTTTGTTGGTTGAAAGAGCAATTCAAGAGGCACATGCCCATCTCAAAGATAAAATCAGATGTAGCAGTGTAGAAAAAGTGCTACCTTCAATGACTCCAGACATATGCATCGCCAACTTTTATACAACCAGTGGGAGGCTTGGTCTTCATCAGGATCGTGATGAAAGCAAAGAGTCTCTCCAAGAAGGATTACCTGTCGTCTCCATTTCTATAGGAGATTCTGCAGATTTCCTCTATGGGGATCAGAGAGAGATTGATAAGGCAGAGAGTGTTGTTTTGGATTCAGGAGATGTGTTAATATTCGGTGGTCATTCAAGACATATATTTCATGGTGTAACATCCATCCTACCAAACTCAGCTGCAAACAGTTTGCTAAAAGAAACTAGGCTTCGTCCTGGCCGTCTGAATCTTACCTTCAGACAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

464

Amino Acids

52.16

Weight (kDa)

6.97

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 244 - 461 3.7e-45 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 929
AciI CCGC 3 cut(s) 67, 120, 508
AclWI GGATC 5 cut(s) 704, 884, 897, 1125, 1212
AcoI YGGCCR 1 cut(s) 1360
AcsI RAATTY 6 cut(s) 72, 467, 526, 616, 674, 959
AcuI CTGAAG 1 cut(s) 1363
AfaI GTAC 3 cut(s) 22, 519, 720
AfiI CCNNNNNNNGG 4 cut(s) 886, 929, 1098, 1201
AflII CTTAAG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 788
AgsI TTSAA 9 cut(s) 375, 532, 629, 745, 776, 977, 989, 1053, 1278
AjnI CCWGG 6 cut(s) 727, 768, 811, 873, 943, 1357
AluBI AGCT 4 cut(s) 632, 799, 853, 1322
AluI AGCT 4 cut(s) 632, 799, 853, 1322
Alw26I GTCTC 3 cut(s) 890, 1143, 1168
AlwI GGATC 5 cut(s) 704, 884, 897, 1125, 1212
AoxI GGCC 3 cut(s) 725, 877, 1360
ApeKI GCWGC 1 cut(s) 1322
ApoI RAATTY 6 cut(s) 72, 467, 526, 616, 674, 959
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 809, 877
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 108
AvaII GGWCC 1 cut(s) 809
BamHI GGATCC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 1100
BbvI GCAGC 1 cut(s) 1309
BccI CCATC 8 cut(s) 290, 498, 602, 646, 688, 923, 1011, 1313
BceAI ACGGC 2 cut(s) 3, 1347
BciT130I CCWGG 6 cut(s) 729, 770, 813, 875, 945, 1359
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 3 cut(s) 890, 1143, 1168
BfaI CTAG 3 cut(s) 800, 900, 1347
BfmI CTRYAG 3 cut(s) 860, 1173, 1185
BfrI CTTAAG 1 cut(s) 151
BisI GCNGC 2 cut(s) 509, 1323
BlsI GCNGC 2 cut(s) 510, 1324
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 7 cut(s) 129, 729, 770, 813, 875, 945, 1359
Bme18I GGWCC 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 809, 877
BmiI GGNNCC 1 cut(s) 891
BmrFI CCNGG 7 cut(s) 129, 729, 770, 813, 875, 945, 1359
BmsI GCATC 3 cut(s) 349, 586, 1080
BpiI GAAGAC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 1044
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 812, 874
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 6 cut(s) 323, 353, 925, 955, 1090, 1120
Bsc4I CCNNNNNNNGG 4 cut(s) 886, 929, 1098, 1201
Bse1I ACTGG 3 cut(s) 713, 830, 1092
BseBI CCWGG 6 cut(s) 729, 770, 813, 875, 945, 1359
BseDI CCNNGG 2 cut(s) 812, 874
BseGI GGATG 7 cut(s) 352, 364, 480, 613, 699, 1301, 1305
BseLI CCNNNNNNNGG 4 cut(s) 886, 929, 1098, 1201
BseMII CTCAG 5 cut(s) 200, 713, 775, 885, 1332
BseNI ACTGG 3 cut(s) 713, 830, 1092
BseXI GCAGC 1 cut(s) 1309
BseYI CCCAGC 1 cut(s) 37
BshFI GGCC 3 cut(s) 727, 879, 1362
BsiSI CCGG 2 cut(s) 83, 129
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 4 cut(s) 886, 929, 1098, 1201
BsmAI GTCTC 3 cut(s) 890, 1143, 1168
BsmBI CGTCTC 1 cut(s) 1168
BsmI GAATGC 2 cut(s) 455, 511
BsnI GGCC 3 cut(s) 727, 879, 1362
Bsp143I GATC 4 cut(s) 709, 889, 1117, 1204
BspACI CCGC 3 cut(s) 67, 120, 508
BspANI GGCC 3 cut(s) 727, 879, 1362
BspCNI CTCAG 5 cut(s) 201, 714, 774, 886, 1331
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 891
BspMAI CTGCAG 2 cut(s) 864, 1189
BspPI GGATC 5 cut(s) 704, 884, 897, 1125, 1212
BspQI GCTCTTC 1 cut(s) 630
BspTI CTTAAG 1 cut(s) 151
BsrI ACTGG 3 cut(s) 713, 830, 1092
BssECI CCNNGG 2 cut(s) 812, 874
BssMI GATC 4 cut(s) 709, 889, 1117, 1204
Bst2UI CCWGG 6 cut(s) 729, 770, 813, 875, 945, 1359
Bst4CI ACNGT 4 cut(s) 591, 670, 1331, 1386
Bst6I CTCTTC 2 cut(s) 377, 630
BstAFI CTTAAG 1 cut(s) 151
BstDEI CTNAG 6 cut(s) 209, 567, 722, 761, 894, 1318
BstF5I GGATG 7 cut(s) 352, 364, 480, 613, 699, 1301, 1305
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 4 cut(s) 712, 892, 1120, 1207
BstMAI GTCTC 3 cut(s) 890, 1143, 1168
BstMBI GATC 4 cut(s) 709, 889, 1117, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 629, 859
BstNI CCWGG 6 cut(s) 729, 770, 813, 875, 945, 1359
BstNSI RCATGY 2 cut(s) 792, 1001
BstSCI CCNGG 7 cut(s) 127, 727, 768, 811, 873, 943, 1357
BstSFI CTRYAG 3 cut(s) 860, 1173, 1185
BstV1I GCAGC 1 cut(s) 1309
BstV2I GAAGAC 1 cut(s) 1100
BstX2I RGATCY 2 cut(s) 709, 889
BstYI RGATCY 2 cut(s) 709, 889
BsuRI GGCC 3 cut(s) 727, 879, 1362
BtgZI GCGATG 1 cut(s) 1057
BtsCI GGATG 7 cut(s) 352, 364, 480, 613, 699, 1301, 1305
BtsI GCAGTG 1 cut(s) 1036
BtsIMutI CAGTG 3 cut(s) 769, 1036, 1099
CciI TCATGA 1 cut(s) 496
CfoI GCGC 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 809, 877
CsiI ACCWGGT 1 cut(s) 768
Csp6I GTAC 3 cut(s) 21, 518, 719
CviAII CATG 7 cut(s) 404, 497, 641, 789, 842, 998, 1292
CviQI GTAC 3 cut(s) 21, 518, 719
DdeI CTNAG 6 cut(s) 209, 567, 722, 761, 894, 1318
DpnI GATC 4 cut(s) 711, 891, 1119, 1206
DpnII GATC 4 cut(s) 709, 889, 1117, 1204
DraI TTTAAA 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 1360
Eam1104I CTCTTC 2 cut(s) 377, 630
EarI CTCTTC 2 cut(s) 377, 630
Eco47I GGWCC 1 cut(s) 809
Eco57I CTGAAG 1 cut(s) 1363
EcoO109I RGGNCCY 1 cut(s) 809
EcoRII CCWGG 6 cut(s) 727, 768, 811, 873, 943, 1357
EcoT22I ATGCAT 1 cut(s) 1073
Esp3I CGTCTC 1 cut(s) 1168
FaeI CATG 7 cut(s) 407, 500, 644, 792, 845, 1001, 1295
FatI CATG 7 cut(s) 403, 496, 640, 788, 841, 997, 1291
FauI CCCGC 2 cut(s) 74, 127
FauNDI CATATG 1 cut(s) 1067
Fnu4HI GCNGC 2 cut(s) 509, 1323
FokI GGATG 7 cut(s) 359, 371, 487, 620, 706, 1288, 1292
Fsp4HI GCNGC 2 cut(s) 509, 1323
FspBI CTAG 3 cut(s) 800, 900, 1347
GlaI GCGC 1 cut(s) 17
GluI GCNGC 2 cut(s) 509, 1323
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 3 cut(s) 727, 879, 1362
HapII CCGG 2 cut(s) 83, 129
HhaI GCGC 1 cut(s) 18
Hin1II CATG 7 cut(s) 407, 500, 644, 792, 845, 1001, 1295
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HindIII AAGCTT 2 cut(s) 630, 851
HinfI GANTC 6 cut(s) 415, 1057, 1136, 1181, 1244, 1369
HpaII CCGG 2 cut(s) 83, 129
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 6 cut(s) 180, 210, 1022, 1209, 1368, 1382
Hpy8I GTNNAC 1 cut(s) 80
Hpy99I CGWCG 2 cut(s) 54, 110
HpyAV CCTTC 4 cut(s) 38, 1059, 1142, 1387
HpyCH4III ACNGT 4 cut(s) 591, 670, 1331, 1386
HpyCH4V TGCA 8 cut(s) 257, 539, 577, 644, 862, 1071, 1187, 1325
HpyF10VI GCNNNNNNNGC 2 cut(s) 629, 859
HpyF3I CTNAG 6 cut(s) 209, 567, 722, 761, 894, 1318
Hsp92II CATG 7 cut(s) 407, 500, 644, 792, 845, 1001, 1295
HspAI GCGC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 709, 889, 1117, 1204
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 1 cut(s) 796
Lsp1109I GCAGC 1 cut(s) 1309
LweI GCATC 3 cut(s) 349, 586, 1080
MabI ACCWGGT 1 cut(s) 768
MaeI CTAG 3 cut(s) 800, 900, 1347
MaeIII GTNAC 5 cut(s) 212, 259, 544, 764, 1297
MalI GATC 4 cut(s) 711, 891, 1119, 1206
MboI GATC 4 cut(s) 709, 889, 1117, 1204
MboII GAAGA 6 cut(s) 38, 394, 617, 858, 923, 1100
MfeI CAATTG 2 cut(s) 246, 440
MflI RGATCY 2 cut(s) 709, 889
MlyI GAGTC 3 cut(s) 424, 1051, 1145
MmeI TCCRAC 2 cut(s) 142, 172
Mph1103I ATGCAT 1 cut(s) 1073
MseI TTAA 3 cut(s) 152, 185, 1259
MslI CAYNNNNRTG 2 cut(s) 495, 582
MspA1I CMGCKG 1 cut(s) 1322
MspCI CTTAAG 1 cut(s) 151
MspI CCGG 2 cut(s) 83, 129
MspR9I CCNGG 7 cut(s) 129, 729, 770, 813, 875, 945, 1359
MunI CAATTG 2 cut(s) 246, 440
Mva1269I GAATGC 2 cut(s) 455, 511
MvaI CCWGG 6 cut(s) 729, 770, 813, 875, 945, 1359
MwoI GCNNNNNNNGC 2 cut(s) 629, 859
NciI CCSGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 1067
NdeII GATC 4 cut(s) 709, 889, 1117, 1204
NlaIII CATG 7 cut(s) 407, 500, 644, 792, 845, 1001, 1295
NlaIV GGNNCC 1 cut(s) 891
NmuCI GTSAC 1 cut(s) 764
NsiI ATGCAT 1 cut(s) 1073
NspI RCATGY 2 cut(s) 792, 1001
PagI TCATGA 1 cut(s) 496
PciI ACATGT 1 cut(s) 788
PciSI GCTCTTC 1 cut(s) 630
PcsI WCGNNNNNNNCGW 1 cut(s) 1360
PctI GAATGC 2 cut(s) 455, 511
PfeI GAWTC 3 cut(s) 1181, 1244, 1369
Pfl23II CGTACG 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 2 cut(s) 510, 1324
PleI GAGTC 3 cut(s) 423, 1051, 1144
PpsI GAGTC 3 cut(s) 423, 1051, 1144
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 788
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 6 cut(s) 727, 768, 811, 873, 943, 1357
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 6 cut(s) 727, 768, 811, 873, 943, 1357
PspLI CGTACG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 891
PspPI GGNCC 2 cut(s) 809, 877
PspPPI RGGWCCY 1 cut(s) 809
PstI CTGCAG 2 cut(s) 864, 1189
PsuI RGATCY 2 cut(s) 709, 889
PvuII CAGCTG 1 cut(s) 1322
RsaI GTAC 3 cut(s) 22, 519, 720
RsaNI GTAC 3 cut(s) 21, 518, 719
RseI CAYNNNNRTG 2 cut(s) 495, 582
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 3 cut(s) 152, 185, 1259
SatI GCNGC 2 cut(s) 509, 1323
Sau3AI GATC 4 cut(s) 709, 889, 1117, 1204
Sau96I GGNCC 2 cut(s) 809, 877
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 3 cut(s) 424, 1051, 1145
ScrFI CCNGG 7 cut(s) 129, 729, 770, 813, 875, 945, 1359
SexAI ACCWGGT 1 cut(s) 768
SfaNI GCATC 3 cut(s) 349, 586, 1080
SfcI CTRYAG 3 cut(s) 860, 1173, 1185
SinI GGWCC 1 cut(s) 809
SmiMI CAYNNNNRTG 2 cut(s) 495, 582
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SsiI CCGC 3 cut(s) 67, 120, 508
SspI AATATT 1 cut(s) 1263
SspMI CTAG 3 cut(s) 800, 900, 1347
StyD4I CCNGG 7 cut(s) 127, 727, 768, 811, 873, 943, 1357
TaaI ACNGT 4 cut(s) 591, 670, 1331, 1386
TaqI TCGA 2 cut(s) 309, 380
TatI WGTACW 1 cut(s) 718
TauI GCSGC 1 cut(s) 511
TfiI GAWTC 3 cut(s) 1181, 1244, 1369
Tru1I TTAA 3 cut(s) 152, 185, 1259
Tru9I TTAA 3 cut(s) 152, 185, 1259
TscAI CASTG 3 cut(s) 769, 1036, 1099
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 1 cut(s) 1322
Tsp45I GTSAC 1 cut(s) 764
TspGWI ACGGA 3 cut(s) 130, 479, 706
TspRI CASTG 3 cut(s) 769, 1036, 1099
Van91I CCANNNNNTGG 1 cut(s) 929
Vha464I CTTAAG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 6 cut(s) 72, 467, 526, 616, 674, 959
XceI RCATGY 2 cut(s) 792, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XspI CTAG 3 cut(s) 800, 900, 1347
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.