RchiOBHm_Chr3g0461361
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007643
Physical Location & Seq
Reverse (-)
9441728 .. 9444217
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42778

Sequence Viewer

Length: 1392 bp
ATGTGGTGGCTCTTGCGCCGTACGACCCTTCTCTCCCCACCCCTTCTTCGTCGTTCATTCCCTCCCCGCCAAATTCGTCCACCGGAAAAAATGAGTTTCAGGAACGACGGGAACTCACCCGCCACTCCCGGTGGCAATTCCGTCATTTCCTTAAGCCCCGTATCAGCGACTAATGTTGGAAACAGAGATTGTCAGATTTTAAAGGTTGGAAGTATTCCTATTCTGATTTACGATAGTTTTGGTAATGTAGTTAAGCGGCCAATTGGGTTTGGAGTTACATCAGGGTTTAGTGATGAGAAGCAATGTGCTTCACTAAGTGATGGTATTCGTTTCGATGAAATGAGATCGAAAGTGGAGGATGCTTGTGTGAGTTCAAATTCGGAGAGTGCAAGTGAAAGGGAACATGAGAGGTGGAGTCCAATGCCGGATTGGAAAAGCCAATTGCGCGAATGCTCTTTGCCTACGGAATTTGGGATGAAAAGGAAGCACCACAGTCGTGCAGAAAAGCGGCATTCTGTACCGAGAAATTTGAAGAATGCACCTGTTACCAAGAGTTCGGGTTTATCTAAGCGTTTGCATTGTTATGAACCATTTGATATATGCTTGGATGGGAGAAATTCTGCTCTTGAAGCTTCTTTTCATGCAAGAATGATGGAAGCAGAGGACACGGTGGAATTTGCTAATCCAGAACGGATGGTGGAATCTAAAGATCCAGTAGTACTGAGGCCAGGAATGGTCTTATTGAAAAATTATGTCAGCCTCAGTCAACAGGTTGAAATTGTAAAGACATGTCGGGAGCTCGGTTTAGGTCCTGGGGGGTTTTATCAACCAGTTTTCCAGCATGGAAGAAAACTTCATCTGCAGATGATGTGCCTGGGCCGGAATTGGGATCCTGAGACTAGGATATATGAAGTTATACGACCAATAGATGACACTCGGCCTCCTGGTATTCCTAATGAATTTAGTTTGTTGGTTGAAAGAGCAATTCAAGAGGCACATGCCCATCTCAAAGATAAAATCATATGTAGCAGTGTAGAAAAAGTGCTACCTTCAATGACTCCAGACATATGCATCGCCAACTTTTATACAACTAGTGGGAGGCTTGGTCTTCATCAGGATCGTGATGAAAGCAAAGAGTCTCTCCAAGAAGGATTACCTGTCGTCTCCATTTCTATAGGAGATTCTGCAGATTTCCTCTATGGGGATCAGAGAGATATTGATAAGGCAGAGAGTGTTGTTTTGGATTCAGGAGATGTGTTAATATTTGGTGGTCGTTCAAGACATATATTTCATGGTGTAACATCCATCATACCAAACTCAGCTCCAAACAATTTGCTAAAAGAAGCTAGGCTTCGTCCTGGCCGTCTGAATCTTACCTTCAGACAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

463

Amino Acids

52.02

Weight (kDa)

8.46

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 244 - 461 1e-45 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 447
AciI CCGC 4 cut(s) 67, 120, 256, 508
AclWI GGATC 5 cut(s) 704, 884, 897, 1125, 1212
AcoI YGGCCR 2 cut(s) 257, 1360
AcsI RAATTY 7 cut(s) 72, 376, 467, 526, 616, 674, 959
AcuI CTGAAG 1 cut(s) 1363
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 3 cut(s) 22, 519, 720
AfiI CCNNNNNNNGG 2 cut(s) 886, 1201
AflII CTTAAG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 788
AgsI TTSAA 9 cut(s) 375, 532, 629, 745, 776, 977, 989, 1053, 1278
AhlI ACTAGT 1 cut(s) 1091
AjnI CCWGG 5 cut(s) 727, 811, 873, 943, 1357
AleI CACNNNNGTG 1 cut(s) 495
AluBI AGCT 4 cut(s) 632, 799, 1322, 1346
AluI AGCT 4 cut(s) 632, 799, 1322, 1346
Alw21I GWGCWC 1 cut(s) 801
Alw26I GTCTC 3 cut(s) 890, 1143, 1168
AlwI GGATC 5 cut(s) 704, 884, 897, 1125, 1212
AoxI GGCC 5 cut(s) 257, 725, 877, 938, 1360
ApoI RAATTY 7 cut(s) 72, 376, 467, 526, 616, 674, 959
AspLEI GCGC 2 cut(s) 18, 447
AspS9I GGNCC 2 cut(s) 809, 877
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 108
AvaII GGWCC 1 cut(s) 809
BamHI GGATCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 1100
Bbv12I GWGCWC 1 cut(s) 801
BccI CCATC 6 cut(s) 314, 602, 646, 688, 1011, 1313
BceAI ACGGC 2 cut(s) 3, 1347
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 5 cut(s) 729, 813, 875, 945, 1359
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 3 cut(s) 890, 1143, 1168
BcuI ACTAGT 1 cut(s) 1091
BfaI CTAG 3 cut(s) 900, 1092, 1347
BfmI CTRYAG 3 cut(s) 860, 1173, 1185
BfrI CTTAAG 1 cut(s) 151
BisI GCNGC 2 cut(s) 257, 509
BlsI GCNGC 2 cut(s) 258, 510
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
Bme18I GGWCC 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 809, 877
BmiI GGNNCC 1 cut(s) 891
BmrFI CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
BmsI GCATC 2 cut(s) 349, 1080
BpiI GAAGAC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 1044
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 812, 874
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 4 cut(s) 925, 955, 1090, 1120
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 1201
Bse1I ACTGG 2 cut(s) 713, 830
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
BseDI CCNNGG 2 cut(s) 812, 874
BseGI GGATG 5 cut(s) 364, 480, 613, 699, 1301
BseLI CCNNNNNNNGG 2 cut(s) 886, 1201
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 4 cut(s) 713, 775, 885, 1332
BseNI ACTGG 2 cut(s) 713, 830
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 5 cut(s) 259, 727, 879, 940, 1362
BsiHKAI GWGCWC 1 cut(s) 801
BsiSI CCGG 4 cut(s) 83, 129, 425, 880
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 886, 1201
BsmAI GTCTC 3 cut(s) 890, 1143, 1168
BsmBI CGTCTC 1 cut(s) 1168
BsmI GAATGC 3 cut(s) 455, 511, 541
BsnI GGCC 5 cut(s) 259, 727, 879, 940, 1362
Bsp1286I GDGCHC 1 cut(s) 801
Bsp143I GATC 5 cut(s) 344, 709, 889, 1117, 1204
BspACI CCGC 4 cut(s) 67, 120, 256, 508
BspANI GGCC 5 cut(s) 259, 727, 879, 940, 1362
BspCNI CTCAG 4 cut(s) 714, 774, 886, 1331
BspFNI CGCG 1 cut(s) 447
BspLI GGNNCC 1 cut(s) 891
BspMAI CTGCAG 2 cut(s) 864, 1189
BspPI GGATC 5 cut(s) 704, 884, 897, 1125, 1212
BspTI CTTAAG 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 308
BsrI ACTGG 2 cut(s) 713, 830
BssECI CCNNGG 2 cut(s) 812, 874
BssMI GATC 5 cut(s) 344, 709, 889, 1117, 1204
Bst2UI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
Bst4CI ACNGT 3 cut(s) 494, 670, 1386
BstAFI CTTAAG 1 cut(s) 151
BstDEI CTNAG 6 cut(s) 314, 567, 722, 761, 894, 1318
BstF5I GGATG 5 cut(s) 364, 480, 613, 699, 1301
BstFNI CGCG 1 cut(s) 447
BstHHI GCGC 2 cut(s) 18, 447
BstKTI GATC 5 cut(s) 347, 712, 892, 1120, 1207
BstMAI GTCTC 3 cut(s) 890, 1143, 1168
BstMBI GATC 5 cut(s) 344, 709, 889, 1117, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 444, 629
BstNI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
BstNSI RCATGY 2 cut(s) 792, 1001
BstSCI CCNGG 6 cut(s) 127, 727, 811, 873, 943, 1357
BstSFI CTRYAG 3 cut(s) 860, 1173, 1185
BstUI CGCG 1 cut(s) 447
BstV2I GAAGAC 1 cut(s) 1100
BstX2I RGATCY 2 cut(s) 709, 889
BstYI RGATCY 2 cut(s) 709, 889
BsuRI GGCC 5 cut(s) 259, 727, 879, 940, 1362
BtgZI GCGATG 1 cut(s) 1057
BtsCI GGATG 5 cut(s) 364, 480, 613, 699, 1301
BtsI GCAGTG 1 cut(s) 1036
BtsIMutI CAGTG 1 cut(s) 1036
CfoI GCGC 2 cut(s) 18, 447
Cfr13I GGNCC 2 cut(s) 809, 877
Csp6I GTAC 3 cut(s) 21, 518, 719
CviAII CATG 6 cut(s) 404, 641, 789, 842, 998, 1292
CviQI GTAC 3 cut(s) 21, 518, 719
DdeI CTNAG 6 cut(s) 314, 567, 722, 761, 894, 1318
DpnI GATC 5 cut(s) 346, 711, 891, 1119, 1206
DpnII GATC 5 cut(s) 344, 709, 889, 1117, 1204
DraI TTTAAA 1 cut(s) 201
DraIII CACNNNGTG 1 cut(s) 317
EaeI YGGCCR 2 cut(s) 257, 1360
Ecl136II GAGCTC 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 801
Eco47I GGWCC 1 cut(s) 809
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 1 cut(s) 1363
EcoICRI GAGCTC 1 cut(s) 799
EcoO109I RGGNCCY 1 cut(s) 809
EcoRII CCWGG 5 cut(s) 727, 811, 873, 943, 1357
EcoT22I ATGCAT 1 cut(s) 1073
EcoT38I GRGCYC 1 cut(s) 801
Esp3I CGTCTC 1 cut(s) 1168
FaeI CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
FalI AAGNNNNNCTT 2 cut(s) 1335, 1367
FatI CATG 6 cut(s) 403, 640, 788, 841, 997, 1291
FauI CCCGC 2 cut(s) 74, 127
FauNDI CATATG 2 cut(s) 1022, 1067
Fnu4HI GCNGC 2 cut(s) 257, 509
FokI GGATG 5 cut(s) 371, 487, 620, 706, 1288
FriOI GRGCYC 1 cut(s) 801
Fsp4HI GCNGC 2 cut(s) 257, 509
FspBI CTAG 3 cut(s) 900, 1092, 1347
GlaI GCGC 2 cut(s) 17, 446
GluI GCNGC 2 cut(s) 257, 509
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 5 cut(s) 259, 727, 879, 940, 1362
HapII CCGG 4 cut(s) 83, 129, 425, 880
HhaI GCGC 2 cut(s) 18, 447
Hin1II CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
Hin6I GCGC 2 cut(s) 16, 445
HinP1I GCGC 2 cut(s) 16, 445
HincII GTYRAC 1 cut(s) 767
HindII GTYRAC 1 cut(s) 767
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 7 cut(s) 415, 701, 1057, 1136, 1181, 1244, 1369
HpaII CCGG 4 cut(s) 83, 129, 425, 880
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 80, 767
Hpy188I TCNGA 6 cut(s) 195, 225, 382, 1209, 1368, 1382
Hpy8I GTNNAC 2 cut(s) 80, 767
Hpy99I CGWCG 2 cut(s) 54, 110
HpyAV CCTTC 5 cut(s) 38, 53, 1059, 1142, 1387
HpyCH4III ACNGT 3 cut(s) 494, 670, 1386
HpyCH4V TGCA 8 cut(s) 389, 500, 539, 577, 644, 862, 1071, 1187
HpyF10VI GCNNNNNNNGC 2 cut(s) 444, 629
HpyF3I CTNAG 6 cut(s) 314, 567, 722, 761, 894, 1318
Hsp92II CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
HspAI GCGC 2 cut(s) 16, 445
Kzo9I GATC 5 cut(s) 344, 709, 889, 1117, 1204
LmnI GCTCC 2 cut(s) 796, 1327
LweI GCATC 2 cut(s) 349, 1080
MaeI CTAG 3 cut(s) 900, 1092, 1347
MaeIII GTNAC 3 cut(s) 274, 544, 1297
MalI GATC 5 cut(s) 346, 711, 891, 1119, 1206
MboI GATC 5 cut(s) 344, 709, 889, 1117, 1204
MboII GAAGA 4 cut(s) 38, 544, 858, 1100
MfeI CAATTG 2 cut(s) 261, 440
MflI RGATCY 2 cut(s) 709, 889
MhlI GDGCHC 1 cut(s) 801
MlyI GAGTC 3 cut(s) 424, 1051, 1145
MmeI TCCRAC 2 cut(s) 157, 187
Mph1103I ATGCAT 1 cut(s) 1073
MseI TTAA 4 cut(s) 152, 200, 252, 1259
MslI CAYNNNNRTG 2 cut(s) 495, 582
MspCI CTTAAG 1 cut(s) 151
MspI CCGG 4 cut(s) 83, 129, 425, 880
MspR9I CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
MunI CAATTG 2 cut(s) 261, 440
Mva1269I GAATGC 3 cut(s) 455, 511, 541
MvaI CCWGG 5 cut(s) 729, 813, 875, 945, 1359
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 444, 629
NciI CCSGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 1022, 1067
NdeII GATC 5 cut(s) 344, 709, 889, 1117, 1204
NlaIII CATG 6 cut(s) 407, 644, 792, 845, 1001, 1295
NlaIV GGNNCC 1 cut(s) 891
NmeAIII GCCGAG 1 cut(s) 916
NsiI ATGCAT 1 cut(s) 1073
NspI RCATGY 2 cut(s) 792, 1001
OliI CACNNNNGTG 1 cut(s) 495
PciI ACATGT 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 1360
PctI GAATGC 3 cut(s) 455, 511, 541
PfeI GAWTC 4 cut(s) 701, 1181, 1244, 1369
Pfl23II CGTACG 1 cut(s) 20
PkrI GCNGC 2 cut(s) 258, 510
PleI GAGTC 3 cut(s) 423, 1051, 1144
PpsI GAGTC 3 cut(s) 423, 1051, 1144
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 5 cut(s) 727, 811, 873, 943, 1357
PspGI CCWGG 5 cut(s) 727, 811, 873, 943, 1357
PspLI CGTACG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 891
PspPI GGNCC 2 cut(s) 809, 877
PspPPI RGGWCCY 1 cut(s) 809
PstI CTGCAG 2 cut(s) 864, 1189
PsuI RGATCY 2 cut(s) 709, 889
RsaI GTAC 3 cut(s) 22, 519, 720
RsaNI GTAC 3 cut(s) 21, 518, 719
RseI CAYNNNNRTG 2 cut(s) 495, 582
SacI GAGCTC 1 cut(s) 801
SaqAI TTAA 4 cut(s) 152, 200, 252, 1259
SatI GCNGC 2 cut(s) 257, 509
Sau3AI GATC 5 cut(s) 344, 709, 889, 1117, 1204
Sau96I GGNCC 2 cut(s) 809, 877
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 3 cut(s) 424, 1051, 1145
ScrFI CCNGG 6 cut(s) 129, 729, 813, 875, 945, 1359
SduI GDGCHC 1 cut(s) 801
SfaNI GCATC 2 cut(s) 349, 1080
SfcI CTRYAG 3 cut(s) 860, 1173, 1185
SinI GGWCC 1 cut(s) 809
SmiMI CAYNNNNRTG 2 cut(s) 495, 582
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SpeI ACTAGT 1 cut(s) 1091
SsiI CCGC 4 cut(s) 67, 120, 256, 508
SspI AATATT 1 cut(s) 1263
SspMI CTAG 3 cut(s) 900, 1092, 1347
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 6 cut(s) 127, 727, 811, 873, 943, 1357
TaaI ACNGT 3 cut(s) 494, 670, 1386
TaqI TCGA 2 cut(s) 333, 347
TatI WGTACW 1 cut(s) 718
TauI GCSGC 2 cut(s) 259, 511
TfiI GAWTC 4 cut(s) 701, 1181, 1244, 1369
Tru1I TTAA 4 cut(s) 152, 200, 252, 1259
Tru9I TTAA 4 cut(s) 152, 200, 252, 1259
TscAI CASTG 1 cut(s) 1036
TspGWI ACGGA 3 cut(s) 130, 479, 706
TspRI CASTG 1 cut(s) 1036
Vha464I CTTAAG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 7 cut(s) 72, 376, 467, 526, 616, 674, 959
XceI RCATGY 2 cut(s) 792, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XspI CTAG 3 cut(s) 900, 1092, 1347
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.