Rroxscaffold_6G00418370
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007643
Physical Location & Seq
Forward (+)
39887076 .. 39889638
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418370.1

Sequence Viewer

Length: 1314 bp
ATGTGGTGGCTCTTGCGCCGTACGACCCTTCTCTCCCCACCCTTTCTTCGTCGTTCATTCCCTCCCCGCCAAATTCGTCCACCGGAAAAAATGAGTTTCAGGAACTCACCCGCCACTCCCGGTGGCAATTCCGTCATTTCCTTAAGCCCCGTATCAGCAGCTAATGTTGGAAACAGAGATTGTCAGATTTTAAAGGTTGGAAGTATTCCTATTCTGATTTACGATAGTTGTGGTAATGTAGTTAAGCGGCCAATTGGGTTTGGAGTTGCATCAGGGTTTAGTGATAAGAAGCAATGTTCTTCAGTAAGTGATGGTATTTGTTTCGATGAAATGAGAACGAAAGTGGAGTTAGTGTCGGGTGATGATGTGGAGGCTGCTTGTATGAGTTCAAATTCGGAGAGTGTAAGTGAAAGGGAACATGAGAGGTGGAGTCCAATGCCGGATTGGAAACGCCAATTGCGTGAATGCTCTTTGCCTACGGAATTTGGGATGAAAAGGAAGCACCATCATCGTGCGGAAAAGCGGCATTCTGTACCGAGAAATTTGAAATATGCACCTGTTACCAAGAGTTCGGGTTTATCTAAGCGTTTGCATCGTTATGAACCGTTTGATATATGCTTGGATGGGAGAAATTCTGCTCTTGAAGCTTCTTTTCATGCAAGAATGATGGAAGCAGAGGACACGGTGGAATCTGCTAATCCAGAACGGATGGTGGAATTTAAAGATCCAATAATACTAAGGCCAGGGATGGTCTTGTTGAAAAATTATGTCAGCCTCTGTGAACAGATGATGTGCCTGGGCCTGAATTGGGATCCTGAGACCAGGATATATGAAGGTATACGACCAATAGATGGCACTCGGCCTCCTGGTATTCCTAATGAATTTAGTTTGTTGGTTGAAAGAGCAATTCAAGAGGCACATGCCCATCTCAAAGATAAAATCAGATGTAGCAGTGTAGAAAAAGTGCTACCTTCAATGACTCCAGACATATGCATCGCCAACTTTTATACAACTAGTGGGAGGCTTGGTCTTCATCAGGATCATGATGAAAGCAAAGAGTCTCTCCAAGAAGGATTACCTGTCGTCTCCATTTCTATAGGAGATTCTGCAGATTTCCTCTATGGGGATCAGAGAGATATTGATAAGGCAGAGAGTGTTGTTTTGGATTCAGGAGATGTGTTAATATTCGGTGGTCGTTCAAGACATATATTTCATGGTGTAACATCCATCATACCAAACTCAGCTCCAAACAATTTGCTAAAAGAAACTAGGCTTCGTCCTGGCCGTCTGAATCTTACCTTCAGACAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

437

Amino Acids

49.14

Weight (kDa)

8.24

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 262 - 435 1.7e-38 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 851
AccI GTMKAC 1 cut(s) 838
AciI CCGC 5 cut(s) 67, 111, 247, 515, 523
AclWI GGATC 5 cut(s) 719, 806, 819, 1047, 1134
AcoI YGGCCR 2 cut(s) 248, 1282
AcsI RAATTY 7 cut(s) 72, 391, 482, 541, 631, 716, 881
AcuI CTGAAG 2 cut(s) 285, 1285
AfaI GTAC 2 cut(s) 22, 534
AfiI CCNNNNNNNGG 3 cut(s) 808, 851, 1123
AflII CTTAAG 1 cut(s) 142
AgsI TTSAA 8 cut(s) 390, 547, 644, 760, 899, 911, 975, 1200
AhlI ACTAGT 1 cut(s) 1013
AjnI CCWGG 5 cut(s) 742, 795, 821, 865, 1279
AluBI AGCT 3 cut(s) 161, 647, 1244
AluI AGCT 3 cut(s) 161, 647, 1244
Alw26I GTCTC 3 cut(s) 812, 1065, 1090
AlwI GGATC 5 cut(s) 719, 806, 819, 1047, 1134
AlwNI CAGNNNCTG 1 cut(s) 777
AoxI GGCC 5 cut(s) 248, 740, 799, 860, 1282
ApeKI GCWGC 2 cut(s) 158, 374
ApoI RAATTY 7 cut(s) 72, 391, 482, 541, 631, 716, 881
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 799
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 99, 371
BamHI GGATCC 1 cut(s) 811
BbsI GAAGAC 1 cut(s) 1022
BbvI GCAGC 2 cut(s) 170, 361
BccI CCATC 9 cut(s) 305, 513, 617, 661, 703, 742, 845, 933, 1235
BceAI ACGGC 2 cut(s) 3, 1269
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 5 cut(s) 744, 797, 823, 867, 1281
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 3 cut(s) 812, 1065, 1090
BcuI ACTAGT 1 cut(s) 1013
BfaI CTAG 2 cut(s) 1014, 1269
BfmI CTRYAG 2 cut(s) 1095, 1107
BfrI CTTAAG 1 cut(s) 142
BisI GCNGC 4 cut(s) 159, 248, 375, 524
BlsI GCNGC 4 cut(s) 160, 249, 376, 525
Bme1390I CCNGG 6 cut(s) 120, 744, 797, 823, 867, 1281
BmgT120I GGNCC 1 cut(s) 799
BmiI GGNNCC 1 cut(s) 813
BmrFI CCNGG 6 cut(s) 120, 744, 797, 823, 867, 1281
BmsI GCATC 3 cut(s) 278, 601, 1002
BpiI GAAGAC 1 cut(s) 1022
BpmI CTGGAG 1 cut(s) 966
BpuMI CCSGG 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 812
BsaJI CCNNGG 2 cut(s) 743, 796
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 6 cut(s) 338, 368, 847, 877, 1012, 1042
Bsc4I CCNNNNNNNGG 3 cut(s) 808, 851, 1123
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 5 cut(s) 744, 797, 823, 867, 1281
BseDI CCNNGG 2 cut(s) 743, 796
BseGI GGATG 5 cut(s) 495, 628, 714, 753, 1223
BseLI CCNNNNNNNGG 3 cut(s) 808, 851, 1123
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 2 cut(s) 807, 1254
BseXI GCAGC 2 cut(s) 170, 361
BshFI GGCC 5 cut(s) 250, 742, 801, 862, 1284
BsiSI CCGG 3 cut(s) 83, 120, 440
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 3 cut(s) 808, 851, 1123
BsmAI GTCTC 3 cut(s) 812, 1065, 1090
BsmBI CGTCTC 1 cut(s) 1090
BsmI GAATGC 2 cut(s) 470, 526
BsnI GGCC 5 cut(s) 250, 742, 801, 862, 1284
Bso31I GGTCTC 1 cut(s) 812
Bsp143I GATC 4 cut(s) 724, 811, 1039, 1126
BspACI CCGC 5 cut(s) 67, 111, 247, 515, 523
BspANI GGCC 5 cut(s) 250, 742, 801, 862, 1284
BspCNI CTCAG 2 cut(s) 808, 1253
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 1 cut(s) 813
BspMAI CTGCAG 1 cut(s) 1111
BspPI GGATC 5 cut(s) 719, 806, 819, 1047, 1134
BspTI CTTAAG 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 743, 796
BssMI GATC 4 cut(s) 724, 811, 1039, 1126
BssNAI GTATAC 1 cut(s) 839
Bst1107I GTATAC 1 cut(s) 839
Bst2UI CCWGG 5 cut(s) 744, 797, 823, 867, 1281
Bst4CI ACNGT 3 cut(s) 606, 685, 1308
BstAFI CTTAAG 1 cut(s) 142
BstDEI CTNAG 4 cut(s) 582, 737, 816, 1240
BstF5I GGATG 5 cut(s) 495, 628, 714, 753, 1223
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 4 cut(s) 727, 814, 1042, 1129
BstMAI GTCTC 3 cut(s) 812, 1065, 1090
BstMBI GATC 4 cut(s) 724, 811, 1039, 1126
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNI CCWGG 5 cut(s) 744, 797, 823, 867, 1281
BstNSI RCATGY 1 cut(s) 923
BstSCI CCNGG 6 cut(s) 118, 742, 795, 821, 865, 1279
BstSFI CTRYAG 2 cut(s) 1095, 1107
BstV1I GCAGC 2 cut(s) 170, 361
BstV2I GAAGAC 1 cut(s) 1022
BstX2I RGATCY 2 cut(s) 724, 811
BstYI RGATCY 2 cut(s) 724, 811
BstZ17I GTATAC 1 cut(s) 839
BsuRI GGCC 5 cut(s) 250, 742, 801, 862, 1284
BtgZI GCGATG 1 cut(s) 979
BtsCI GGATG 5 cut(s) 495, 628, 714, 753, 1223
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 1 cut(s) 958
CaiI CAGNNNCTG 1 cut(s) 777
CciI TCATGA 1 cut(s) 1042
CfoI GCGC 1 cut(s) 18
Cfr13I GGNCC 1 cut(s) 799
Csp6I GTAC 2 cut(s) 21, 533
CviAII CATG 5 cut(s) 419, 656, 920, 1043, 1214
CviQI GTAC 2 cut(s) 21, 533
DdeI CTNAG 4 cut(s) 582, 737, 816, 1240
DpnI GATC 4 cut(s) 726, 813, 1041, 1128
DpnII GATC 4 cut(s) 724, 811, 1039, 1126
DraI TTTAAA 2 cut(s) 192, 721
EaeI YGGCCR 2 cut(s) 248, 1282
Eco31I GGTCTC 1 cut(s) 812
Eco57I CTGAAG 2 cut(s) 285, 1285
EcoRII CCWGG 5 cut(s) 742, 795, 821, 865, 1279
EcoT22I ATGCAT 1 cut(s) 995
Esp3I CGTCTC 1 cut(s) 1090
FaeI CATG 5 cut(s) 422, 659, 923, 1046, 1217
FatI CATG 5 cut(s) 418, 655, 919, 1042, 1213
FauI CCCGC 2 cut(s) 74, 118
FauNDI CATATG 1 cut(s) 989
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 4 cut(s) 159, 248, 375, 524
FokI GGATG 5 cut(s) 502, 635, 721, 760, 1210
Fsp4HI GCNGC 4 cut(s) 159, 248, 375, 524
FspBI CTAG 2 cut(s) 1014, 1269
GlaI GCGC 1 cut(s) 17
GluI GCNGC 4 cut(s) 159, 248, 375, 524
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 5 cut(s) 250, 742, 801, 862, 1284
HapII CCGG 3 cut(s) 83, 120, 440
HhaI GCGC 1 cut(s) 18
Hin1II CATG 5 cut(s) 422, 659, 923, 1046, 1217
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 7 cut(s) 430, 689, 979, 1058, 1103, 1166, 1291
HpaII CCGG 3 cut(s) 83, 120, 440
HphI GGTGA 2 cut(s) 99, 371
Hpy166II GTNNAC 3 cut(s) 80, 782, 839
Hpy188I TCNGA 7 cut(s) 186, 216, 397, 944, 1131, 1290, 1304
Hpy8I GTNNAC 3 cut(s) 80, 782, 839
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 5 cut(s) 38, 827, 981, 1064, 1309
HpyCH4III ACNGT 3 cut(s) 606, 685, 1308
HpyCH4V TGCA 6 cut(s) 269, 554, 592, 659, 993, 1109
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 4 cut(s) 582, 737, 816, 1240
Hsp92II CATG 5 cut(s) 422, 659, 923, 1046, 1217
HspAI GCGC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 724, 811, 1039, 1126
LmnI GCTCC 1 cut(s) 1249
Lsp1109I GCAGC 2 cut(s) 170, 361
LweI GCATC 3 cut(s) 278, 601, 1002
MaeI CTAG 2 cut(s) 1014, 1269
MaeIII GTNAC 2 cut(s) 559, 1219
MalI GATC 4 cut(s) 726, 813, 1041, 1128
MboI GATC 4 cut(s) 724, 811, 1039, 1126
MboII GAAGA 3 cut(s) 38, 291, 1022
MfeI CAATTG 2 cut(s) 252, 455
MflI RGATCY 2 cut(s) 724, 811
MlyI GAGTC 3 cut(s) 439, 973, 1067
MmeI TCCRAC 2 cut(s) 148, 178
MnlI CCTC 9 cut(s) 72, 364, 417, 670, 785, 873, 907, 1014, 1127
Mph1103I ATGCAT 1 cut(s) 995
MseI TTAA 5 cut(s) 143, 191, 243, 720, 1181
MslI CAYNNNNRTG 2 cut(s) 510, 597
MspCI CTTAAG 1 cut(s) 142
MspI CCGG 3 cut(s) 83, 120, 440
MspR9I CCNGG 6 cut(s) 120, 744, 797, 823, 867, 1281
MunI CAATTG 2 cut(s) 252, 455
Mva1269I GAATGC 2 cut(s) 470, 526
MvaI CCWGG 5 cut(s) 744, 797, 823, 867, 1281
MwoI GCNNNNNNNGC 1 cut(s) 644
NciI CCSGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 989
NdeII GATC 4 cut(s) 724, 811, 1039, 1126
NlaIII CATG 5 cut(s) 422, 659, 923, 1046, 1217
NlaIV GGNNCC 1 cut(s) 813
NmeAIII GCCGAG 1 cut(s) 838
NsiI ATGCAT 1 cut(s) 995
NspI RCATGY 1 cut(s) 923
PagI TCATGA 1 cut(s) 1042
PcsI WCGNNNNNNNCGW 2 cut(s) 457, 1282
PctI GAATGC 2 cut(s) 470, 526
PfeI GAWTC 4 cut(s) 689, 1103, 1166, 1291
Pfl23II CGTACG 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 4 cut(s) 160, 249, 376, 525
PleI GAGTC 3 cut(s) 438, 973, 1066
PpsI GAGTC 3 cut(s) 438, 973, 1066
Psp6I CCWGG 5 cut(s) 742, 795, 821, 865, 1279
PspGI CCWGG 5 cut(s) 742, 795, 821, 865, 1279
PspLI CGTACG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 813
PspPI GGNCC 1 cut(s) 799
PstI CTGCAG 1 cut(s) 1111
PstNI CAGNNNCTG 1 cut(s) 777
PsuI RGATCY 2 cut(s) 724, 811
RsaI GTAC 2 cut(s) 22, 534
RsaNI GTAC 2 cut(s) 21, 533
RseI CAYNNNNRTG 2 cut(s) 510, 597
SaqAI TTAA 5 cut(s) 143, 191, 243, 720, 1181
SatI GCNGC 4 cut(s) 159, 248, 375, 524
Sau3AI GATC 4 cut(s) 724, 811, 1039, 1126
Sau96I GGNCC 1 cut(s) 799
SchI GAGTC 3 cut(s) 439, 973, 1067
ScrFI CCNGG 6 cut(s) 120, 744, 797, 823, 867, 1281
SfaNI GCATC 3 cut(s) 278, 601, 1002
SfcI CTRYAG 2 cut(s) 1095, 1107
SmiMI CAYNNNNRTG 2 cut(s) 510, 597
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
SpeI ACTAGT 1 cut(s) 1013
SsiI CCGC 5 cut(s) 67, 111, 247, 515, 523
SspI AATATT 1 cut(s) 1185
SspMI CTAG 2 cut(s) 1014, 1269
StyD4I CCNGG 6 cut(s) 118, 742, 795, 821, 865, 1279
TaaI ACNGT 3 cut(s) 606, 685, 1308
TaqI TCGA 1 cut(s) 324
TauI GCSGC 2 cut(s) 250, 526
TfiI GAWTC 4 cut(s) 689, 1103, 1166, 1291
Tru1I TTAA 5 cut(s) 143, 191, 243, 720, 1181
Tru9I TTAA 5 cut(s) 143, 191, 243, 720, 1181
TscAI CASTG 1 cut(s) 958
TseI GCWGC 2 cut(s) 158, 374
TspGWI ACGGA 3 cut(s) 121, 494, 721
TspRI CASTG 1 cut(s) 958
Van91I CCANNNNNTGG 1 cut(s) 851
Vha464I CTTAAG 1 cut(s) 142
XapI RAATTY 7 cut(s) 72, 391, 482, 541, 631, 716, 881
XceI RCATGY 1 cut(s) 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 838
XspI CTAG 2 cut(s) 1014, 1269
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.