Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005975

Physical Location & Seq

Genomic Coordinates Forward (+)

28594072 .. 28598647

Gene Structure 4576 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1205400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGAGGGTTTTGGAGGTTTGGGATTCAGCGATGGAAGCAATGCTGTGAGGAAGAAAAGGAGTAACACGTCTCGTCGTCCTCGGAACGAGTCACAGCTACCTTTGGATTGTCGTGATAATGTGTCATCAACTCCTTCTTCGGAAGATGTAAGCAAAAGTGATGAGAATAACGATGATGGTTCAATTACACGAAAGAGAGACATTAATTTGAACTTATGCAGCGCAAGGGCTTCGTTTAGTAACATTATGGAAGCTGAGACTGTCCAGAATATGGATAAAGGTGAGGGAGGAGGTTCCGGAGAATCTGAGGAAGCTTCAAATGATGGTTCATTTCGAGGCAGTGACGAACAGAGGCATAGGGGGATTGATTCCAAAAGGTCCAATAAGGGTGCTCTTGCCCCCGCTAATTGGAAAGGCACAAACAAGCTTGGGCCTGTTGGAGTTGTTTCGGATGGCTCAGAGAATGAGAATAAAGTGAAAATGGTTAAGCTTAAAGTTGGTGGTGTTACACGCACAATTCAGGCCAAGTCTACATCTGGTGGTGCATCTACTGTTGGTCCGTCTTCTGTAAAATCTTCTCGCATTTCAGATGCCCCTCGACCACGCCAGAAGTTGATTCTTCAGGAAGACTCGGATGATAGTCCTTCCCTTAGTTCAGATAAAGGAAGTGGGTTACGGGGAGTCCCACGGAAGGATACATCTAAAACTGTCACTCGTGTTGGGAAGGCTGAAGAACCTGTTCGCAAGAGCAAACGGGTCCCTAAGAGACGCGTCCTGGATGCAGTTGATGATGGAGATGATGATGACATGGAAGTTCGATACCTTGAAAAACTCAAAATATCTAAGGTCACCTCAGATTATACTGCAGAGTACAATGAGGATGAGGAAAGGAAAACTAGAAAAGAAAGGAAGATTTCGACAGTGATGAAGGGGAGTGGTATTGGACTAAGTGTAGATTTGGGAGATTATGGCATACCAAGATCAGGCAAGGATGGTAAGAAGTCCAGAGTAGGAAGCGTATCTGATGATACTGATTATGTTGAAGAGGAAGAAGAACCTGTTTCTGATGGTGAGCCTAATACTAAAAGCAAAAAATCGAGAAAGGACTTTGTTGAATCATCCTCATATAATAAGAAGGAGATGACAGTCACTACTCGTCAAAGGGCTCTTAACACTGGCAAAGATGTCTCATCCAGCTCAGGTGCAGGTTTATTTGAGTTCCCAAATGGGTTGCCCCCGGCTCCACCAAGAAAGCAAAAGGAGAAACCCTGTGCATTGGAGCAGCAAATCAAGAAAGCTGAGGCGGCGGAGAGACGTAGGATGCAAGTAGAGAAGGCAACTCGAGAATCTGAGGCTGAGGCAATCAGAAAAATTCTGGGGCAAGATTCCAGTAGGAAGAAACGAGAAGACAAGATAAAGAAACGGCAAGAAGATATGGCTCAGGAGAGGGCTGCAAATGCTTTCACACTTCCGCCAGATTCTGTCCGATGGGTGATGGGTCCATCAGGATCGGTTGTAACTTTCCCCAATGAAATGGGCTTGCCAGCTATATTCGACTCCAAGCCTTGCAGTTACCCTCCTCCCCGAGAAAAGTGTGCCGGACCATATTGTACAAATCCTTACAAGTACCGGGATTCACAGTCGAAGCTCCCTTTGTGCAGTCTCCGGTGTTACAAGGCAATACATGAAAAGATGGCCCCTCTATCTGCCTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PAPA-1 zf-HIT

Protein Analysis

572

Amino Acids

62.56

Weight (kDa)

9.2

Isoelectric Point (pI)

51.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAPA-1	PF04795	427 - 512	9.1e-22	PAPA-1-like conserved region
zf-HIT	PF04438	528 - 559	3e-07	HIT zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56460 AT1G56460 AT1G56460
fragaria_vesca	FvH4_6g07480 FvH4_6g07480 FvH4_6g07480 FvH4_6g07480
malus_domestica	MD00G1208200.v1.1 MD04G1214800.v1.1 MD04G1215000.v1.1 MD12G1205400.v1.1
prunus_persica	Prupe.6G312500_v2.0.a1
pyrus_communis	pycom12g19330
rosa_chinensis	RchiOBHm_Chr3g0457151 RchiOBHm_Chr3g0457181
rosa_laevigata	RLG00000025244
rosa_multiflora	Rmu_sc0000488.1_g000012
rosa_roxburghii	Rroxscaffold_6G00422130
rosa_rugosa	Rorug03G0019200 Rorug03G0019300 Rorug03G0019300 Rorug03G0019400
rosa_samantha	Rh3AG079100 Rh3BG080800 Rh3CG080900 Rh3DG082800
rosa_wichuraiana	Rw3G006800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1196
AccB7I	CCANNNNNTGG	1 cut(s)	271
AccI	GTMKAC	1 cut(s)	530
AccII	CGCG	1 cut(s)	771
AccIII	TCCGGA	1 cut(s)	296
AciI	CCGC	4 cut(s)	402, 1304, 1307, 1472
AclWI	GGATC	1 cut(s)	1516
AcsI	RAATTY	1 cut(s)	1371
AcuI	CTGAAG	2 cut(s)	605, 750
AfaI	GTAC	3 cut(s)	872, 1612, 1628
AfiI	CCNNNNNNNGG	4 cut(s)	271, 408, 691, 983
AflIII	ACRYGT	2 cut(s)	66, 769
AgsI	TTSAA	6 cut(s)	183, 211, 318, 827, 1043, 1115
AjiI	CACGTC	1 cut(s)	69
AjnI	CCWGG	1 cut(s)	774
AluBI	AGCT	9 cut(s)	97, 254, 314, 427, 490, 1197, 1298, 1547, 1648
AluI	AGCT	9 cut(s)	97, 254, 314, 427, 490, 1197, 1298, 1547, 1648
Alw21I	GWGCWC	1 cut(s)	394
Alw26I	GTCTC	7 cut(s)	75, 192, 251, 760, 1192, 1306, 1667
AlwI	GGATC	1 cut(s)	1516
AlwNI	CAGNNNCTG	1 cut(s)	1481
Ama87I	CYCGRG	2 cut(s)	1341, 1584
Aor13HI	TCCGGA	1 cut(s)	296
AoxI	GGCC	3 cut(s)	431, 522, 1695
ApeKI	GCWGC	3 cut(s)	219, 1282, 1451
ApoI	RAATTY	1 cut(s)	1371
AseI	ATTAAT	1 cut(s)	204
Asp700I	GAANNNNTTC	1 cut(s)	738
AspLEI	GCGC	1 cut(s)	224
AspS9I	GGNCC	7 cut(s)	378, 431, 557, 757, 1499, 1601, 1696
AsuC2I	CCSGG	2 cut(s)	1238, 1631
AsuHPI	GGTGA	4 cut(s)	293, 841, 1082, 1504
AvaI	CYCGRG	2 cut(s)	1341, 1584
AvaII	GGWCC	5 cut(s)	378, 557, 757, 1499, 1601
BanII	GRGCYC	1 cut(s)	1168
BauI	CACGAG	1 cut(s)	714
BbsI	GAAGAC	3 cut(s)	555, 633, 1413
Bbv12I	GWGCWC	1 cut(s)	394
BbvCI	CCTCAGC	2 cut(s)	1299, 1356
BbvI	GCAGC	3 cut(s)	231, 1294, 1438
BceAI	ACGGC	1 cut(s)	1439
BcgI	CGANNNNNNTGC	2 cut(s)	213, 247
BciT130I	CCWGG	1 cut(s)	776
BciVI	GTATCC	1 cut(s)	688
BcnI	CCSGG	2 cut(s)	1238, 1631
BcoDI	GTCTC	7 cut(s)	75, 192, 251, 760, 1192, 1306, 1667
BfaI	CTAG	1 cut(s)	897
BfmI	CTRYAG	1 cut(s)	864
BfuAI	ACCTGC	1 cut(s)	1196
BfuI	GTATCC	1 cut(s)	688
BisI	GCNGC	4 cut(s)	220, 1283, 1305, 1452
BlsI	GCNGC	4 cut(s)	221, 1284, 1306, 1453
Bme1390I	CCNGG	3 cut(s)	776, 1238, 1631
Bme18I	GGWCC	5 cut(s)	378, 557, 757, 1499, 1601
BmeT110I	CYCGRG	2 cut(s)	1341, 1584
BmgBI	CACGTC	1 cut(s)	69
BmgT120I	GGNCC	7 cut(s)	378, 431, 557, 757, 1499, 1601, 1696
BmiI	GGNNCC	6 cut(s)	295, 758, 759, 1242, 1500, 1698
BmrFI	CCNGG	3 cut(s)	776, 1238, 1631
BmsI	GCATC	4 cut(s)	554, 580, 769, 1311
BpiI	GAAGAC	3 cut(s)	555, 633, 1413
Bpu10I	CCTNAGC	4 cut(s)	1198, 1299, 1356, 1440
BpuMI	CCSGG	2 cut(s)	1238, 1631
BsaJI	CCNNGG	3 cut(s)	80, 686, 1236
BsaWI	WCCGGW	2 cut(s)	296, 1665
Bsc4I	CCNNNNNNNGG	4 cut(s)	271, 408, 691, 983
Bse1I	ACTGG	2 cut(s)	1180, 1389
Bse3DI	GCAATG	1 cut(s)	46
BseAI	TCCGGA	1 cut(s)	296
BseBI	CCWGG	1 cut(s)	776
BseDI	CCNNGG	3 cut(s)	80, 686, 1236
BseGI	GGATG	8 cut(s)	457, 640, 784, 886, 997, 1118, 1190, 1326
BseLI	CCNNNNNNNGG	4 cut(s)	271, 408, 691, 983
BseMI	GCAATG	1 cut(s)	46
BseMII	CTCAG	9 cut(s)	246, 297, 471, 867, 1212, 1290, 1341, 1347, 1454
BseNI	ACTGG	2 cut(s)	1180, 1389
BseRI	GAGGAG	2 cut(s)	303, 1569
BseXI	GCAGC	3 cut(s)	231, 1294, 1438
BsgI	GTGCAG	2 cut(s)	1224, 1678
Bsh1236I	CGCG	1 cut(s)	771
BshFI	GGCC	3 cut(s)	433, 524, 1697
BsiHKAI	GWGCWC	1 cut(s)	394
BsiHKCI	CYCGRG	2 cut(s)	1341, 1584
BsiSI	CCGG	5 cut(s)	297, 1238, 1599, 1630, 1666
BslFI	GGGAC	2 cut(s)	668, 743
BslI	CCNNNNNNNGG	4 cut(s)	271, 408, 691, 983
BsmAI	GTCTC	7 cut(s)	75, 192, 251, 760, 1192, 1306, 1667
BsmBI	CGTCTC	3 cut(s)	75, 760, 1306
BsmFI	GGGAC	2 cut(s)	668, 743
BsnI	GGCC	3 cut(s)	433, 524, 1697
BsoBI	CYCGRG	2 cut(s)	1341, 1584
Bsp1286I	GDGCHC	2 cut(s)	394, 1168
Bsp13I	TCCGGA	1 cut(s)	296
Bsp1407I	TGTACA	1 cut(s)	1610
Bsp143I	GATC	2 cut(s)	980, 1508
BspACI	CCGC	4 cut(s)	402, 1304, 1307, 1472
BspANI	GGCC	3 cut(s)	433, 524, 1697
BspCNI	CTCAG	9 cut(s)	247, 298, 470, 866, 1211, 1291, 1342, 1348, 1453
BspEI	TCCGGA	1 cut(s)	296
BspFNI	CGCG	1 cut(s)	771
BspLI	GGNNCC	6 cut(s)	295, 758, 759, 1242, 1500, 1698
BspMAI	CTGCAG	1 cut(s)	868
BspMI	ACCTGC	1 cut(s)	1196
BspPI	GGATC	1 cut(s)	1516
BsrDI	GCAATG	1 cut(s)	46
BsrGI	TGTACA	1 cut(s)	1610
BsrI	ACTGG	2 cut(s)	1180, 1389
BssECI	CCNNGG	3 cut(s)	80, 686, 1236
BssMI	GATC	2 cut(s)	980, 1508
BssSI	CACGAG	1 cut(s)	714
Bst2BI	CACGAG	1 cut(s)	714
Bst2UI	CCWGG	1 cut(s)	776
Bst4CI	ACNGT	6 cut(s)	262, 553, 709, 922, 1147, 1641
Bst6I	CTCTTC	1 cut(s)	1038
BstAUI	TGTACA	1 cut(s)	1610
BstC8I	GCNNGC	2 cut(s)	1541, 1545
BstDSI	CCRYGG	1 cut(s)	686
BstEII	GGTNACC	1 cut(s)	847
BstF5I	GGATG	8 cut(s)	457, 640, 784, 886, 997, 1118, 1190, 1326
BstFNI	CGCG	1 cut(s)	771
BstHHI	GCGC	1 cut(s)	224
BstKTI	GATC	2 cut(s)	983, 1511
BstMAI	GTCTC	7 cut(s)	75, 192, 251, 760, 1192, 1306, 1667
BstMBI	GATC	2 cut(s)	980, 1508
BstMWI	GCNNNNNNNGC	3 cut(s)	36, 1304, 1457
BstNI	CCWGG	1 cut(s)	776
BstPI	GGTNACC	1 cut(s)	847
BstSCI	CCNGG	3 cut(s)	774, 1236, 1629
BstSFI	CTRYAG	1 cut(s)	864
BstUI	CGCG	1 cut(s)	771
BstV1I	GCAGC	3 cut(s)	231, 1294, 1438
BstV2I	GAAGAC	3 cut(s)	555, 633, 1413
BstXI	CCANNNNNNTGG	1 cut(s)	1534
BsuI	GTATCC	1 cut(s)	688
BsuRI	GGCC	3 cut(s)	433, 524, 1697
BtgI	CCRYGG	1 cut(s)	686
BtgZI	GCGATG	1 cut(s)	45
BtrI	CACGTC	1 cut(s)	69
BtsCI	GGATG	8 cut(s)	457, 640, 784, 886, 997, 1118, 1190, 1326
BtsI	GCAGTG	1 cut(s)	346
BtsIMutI	CAGTG	3 cut(s)	346, 927, 1173
BveI	ACCTGC	1 cut(s)	1196
Cac8I	GCNNGC	2 cut(s)	1541, 1545
CaiI	CAGNNNCTG	1 cut(s)	1481
CfoI	GCGC	1 cut(s)	224
Cfr13I	GGNCC	7 cut(s)	378, 431, 557, 757, 1499, 1601, 1696
CseI	GACGC	2 cut(s)	760, 777
Csp6I	GTAC	3 cut(s)	871, 1611, 1627
CviAII	CATG	2 cut(s)	808, 1685
CviQI	GTAC	3 cut(s)	871, 1611, 1627
DpnI	GATC	2 cut(s)	982, 1510
DpnII	GATC	2 cut(s)	980, 1508
Eam1104I	CTCTTC	1 cut(s)	1038
EarI	CTCTTC	1 cut(s)	1038
EciI	GGCGGA	2 cut(s)	1322, 1461
Eco24I	GRGCYC	1 cut(s)	1168
Eco47I	GGWCC	5 cut(s)	378, 557, 757, 1499, 1601
Eco57I	CTGAAG	2 cut(s)	605, 750
Eco88I	CYCGRG	2 cut(s)	1341, 1584
Eco91I	GGTNACC	1 cut(s)	847
EcoO109I	RGGNCCY	1 cut(s)	757
EcoO65I	GGTNACC	1 cut(s)	847
EcoRII	CCWGG	1 cut(s)	774
EcoT38I	GRGCYC	1 cut(s)	1168
Esp3I	CGTCTC	3 cut(s)	75, 760, 1306
FaeI	CATG	2 cut(s)	811, 1688
FaqI	GGGAC	2 cut(s)	668, 743
FatI	CATG	2 cut(s)	807, 1684
FauI	CCCGC	1 cut(s)	409
FblI	GTMKAC	1 cut(s)	530
Fnu4HI	GCNGC	4 cut(s)	220, 1283, 1305, 1452
FokI	GGATG	8 cut(s)	464, 647, 791, 893, 1004, 1105, 1177, 1333
FriOI	GRGCYC	1 cut(s)	1168
Fsp4HI	GCNGC	4 cut(s)	220, 1283, 1305, 1452
FspBI	CTAG	1 cut(s)	897
GlaI	GCGC	1 cut(s)	223
GluI	GCNGC	4 cut(s)	220, 1283, 1305, 1452
HaeIII	GGCC	3 cut(s)	433, 524, 1697
HapII	CCGG	5 cut(s)	297, 1238, 1599, 1630, 1666
HgaI	GACGC	2 cut(s)	760, 777
HhaI	GCGC	1 cut(s)	224
Hin1II	CATG	2 cut(s)	811, 1688
Hin6I	GCGC	1 cut(s)	222
HinP1I	GCGC	1 cut(s)	222
HindIII	AAGCTT	3 cut(s)	312, 425, 488
HpaII	CCGG	5 cut(s)	297, 1238, 1599, 1630, 1666
HphI	GGTGA	4 cut(s)	293, 841, 1082, 1504
Hpy166II	GTNNAC	1 cut(s)	531
Hpy8I	GTNNAC	1 cut(s)	531
Hpy99I	CGWCG	1 cut(s)	78
HpyAV	CCTTC	7 cut(s)	144, 654, 685, 718, 922, 1129, 1327
HpyCH4III	ACNGT	6 cut(s)	262, 553, 709, 922, 1147, 1641
HpyCH4IV	ACGT	2 cut(s)	68, 1315
HpyF10VI	GCNNNNNNNGC	3 cut(s)	36, 1304, 1457
HpySE526I	ACGT	2 cut(s)	68, 1315
Hsp92II	CATG	2 cut(s)	811, 1688
HspAI	GCGC	1 cut(s)	222
KflI	GGGWCCC	1 cut(s)	757
Kpn2I	TCCGGA	1 cut(s)	296
Kzo9I	GATC	2 cut(s)	980, 1508
LmnI	GCTCC	3 cut(s)	1246, 1279, 1653
Lsp1109I	GCAGC	3 cut(s)	231, 1294, 1438
LweI	GCATC	4 cut(s)	554, 580, 769, 1311
MaeI	CTAG	1 cut(s)	897
MaeII	ACGT	2 cut(s)	68, 1315
MalI	GATC	2 cut(s)	982, 1510
MboI	GATC	2 cut(s)	980, 1508
MhlI	GDGCHC	2 cut(s)	394, 1168
MluCI	AATT	5 cut(s)	183, 205, 406, 516, 1371
MluI	ACGCGT	1 cut(s)	769
MlyI	GAGTC	4 cut(s)	98, 623, 690, 1550
MmeI	TCCRAC	1 cut(s)	418
MroI	TCCGGA	1 cut(s)	296
MroXI	GAANNNNTTC	1 cut(s)	738
MseI	TTAA	4 cut(s)	204, 486, 492, 1170
MspI	CCGG	5 cut(s)	297, 1238, 1599, 1630, 1666
MspR9I	CCNGG	3 cut(s)	776, 1238, 1631
MvaI	CCWGG	1 cut(s)	776
MvnI	CGCG	1 cut(s)	771
MwoI	GCNNNNNNNGC	3 cut(s)	36, 1304, 1457
NciI	CCSGG	2 cut(s)	1238, 1631
NdeII	GATC	2 cut(s)	980, 1508
NlaIII	CATG	2 cut(s)	811, 1688
NlaIV	GGNNCC	6 cut(s)	295, 758, 759, 1242, 1500, 1698
NmuCI	GTSAC	5 cut(s)	90, 341, 709, 847, 1147
PaeR7I	CTCGAG	1 cut(s)	1341
PdmI	GAANNNNTTC	1 cut(s)	738
PfeI	GAWTC	9 cut(s)	24, 302, 368, 616, 1115, 1346, 1385, 1478, 1634
PflMI	CCANNNNNTGG	1 cut(s)	271
PfoI	TCCNGGA	1 cut(s)	774
PkrI	GCNGC	4 cut(s)	221, 1284, 1306, 1453
PleI	GAGTC	4 cut(s)	97, 623, 689, 1550
PpsI	GAGTC	4 cut(s)	97, 623, 689, 1550
PpuMI	RGGWCCY	1 cut(s)	757
PshBI	ATTAAT	1 cut(s)	204
Psp5II	RGGWCCY	1 cut(s)	757
Psp6I	CCWGG	1 cut(s)	774
PspEI	GGTNACC	1 cut(s)	847
PspGI	CCWGG	1 cut(s)	774
PspN4I	GGNNCC	6 cut(s)	295, 758, 759, 1242, 1500, 1698
PspPI	GGNCC	7 cut(s)	378, 431, 557, 757, 1499, 1601, 1696
PspPPI	RGGWCCY	1 cut(s)	757
PstI	CTGCAG	1 cut(s)	868
PstNI	CAGNNNCTG	1 cut(s)	1481
RsaI	GTAC	3 cut(s)	872, 1612, 1628
RsaNI	GTAC	3 cut(s)	871, 1611, 1627
SaqAI	TTAA	4 cut(s)	204, 486, 492, 1170
SatI	GCNGC	4 cut(s)	220, 1283, 1305, 1452
Sau3AI	GATC	2 cut(s)	980, 1508
Sau96I	GGNCC	7 cut(s)	378, 431, 557, 757, 1499, 1601, 1696
SchI	GAGTC	4 cut(s)	98, 623, 690, 1550
ScrFI	CCNGG	3 cut(s)	776, 1238, 1631
SduI	GDGCHC	2 cut(s)	394, 1168
SfaNI	GCATC	4 cut(s)	554, 580, 769, 1311
SfcI	CTRYAG	1 cut(s)	864
Sfr274I	CTCGAG	1 cut(s)	1341
SinI	GGWCC	5 cut(s)	378, 557, 757, 1499, 1601
SlaI	CTCGAG	1 cut(s)	1341
SmlI	CTYRAG	1 cut(s)	1341
SmoI	CTYRAG	1 cut(s)	1341
Sse9I	AATT	5 cut(s)	183, 205, 406, 516, 1371
SsiI	CCGC	4 cut(s)	402, 1304, 1307, 1472
SspMI	CTAG	1 cut(s)	897
StyD4I	CCNGG	3 cut(s)	774, 1236, 1629
TaaI	ACNGT	6 cut(s)	262, 553, 709, 922, 1147, 1641
TaiI	ACGT	2 cut(s)	71, 1318
TaqI	TCGA	8 cut(s)	334, 598, 817, 917, 1097, 1342, 1554, 1643
TasI	AATT	5 cut(s)	183, 205, 406, 516, 1371
TatI	WGTACW	2 cut(s)	870, 1610
TauI	GCSGC	1 cut(s)	1307
TfiI	GAWTC	9 cut(s)	24, 302, 368, 616, 1115, 1346, 1385, 1478, 1634
Tru1I	TTAA	4 cut(s)	204, 486, 492, 1170
Tru9I	TTAA	4 cut(s)	204, 486, 492, 1170
TscAI	CASTG	3 cut(s)	346, 927, 1180
TseFI	GTSAC	5 cut(s)	90, 341, 709, 847, 1147
TseI	GCWGC	3 cut(s)	219, 1282, 1451
Tsp45I	GTSAC	5 cut(s)	90, 341, 709, 847, 1147
TspDTI	ATGAA	4 cut(s)	318, 941, 1545, 1701
TspGWI	ACGGA	2 cut(s)	549, 703
TspRI	CASTG	3 cut(s)	346, 927, 1180
Van91I	CCANNNNNTGG	1 cut(s)	271
VpaK11BI	GGWCC	5 cut(s)	378, 557, 757, 1499, 1601
VspI	ATTAAT	1 cut(s)	204
XapI	RAATTY	1 cut(s)	1371
XhoI	CTCGAG	1 cut(s)	1341
XmiI	GTMKAC	1 cut(s)	530
XmnI	GAANNNNTTC	1 cut(s)	738
XspI	CTAG	1 cut(s)	897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.