Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005975

Physical Location & Seq

Genomic Coordinates Forward (+)

43347867 .. 43350327

Gene Structure 2461 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025244

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGAGAGTTTCAGCGGCTTGGGATTCAGTGAAGTAAACAGTGCTGCAAGGAGGAAAAGGAGCAATACATCTCGTCGTCCTCGGAATGAGTCACAGCTTCCTATGGATTATCGTGACATCTCTTCACTGTCATCAACTCCTCCTTCAGACGATAATATGATTAAATCTGAGGGAGGAGGATACGTTGAATCTGATGAAGCTTCAAATAATGGTTCTTTTCGAGGCAGTAATGAGCATAGGCATACTGGGATTGATTCCAAAAGGTCAAGCATGGGTGTCCTTGGCCCTGCTAATAGGAAAGGCCCCAGCAAGGATGGGTCTTTTGGTATTGTTTCTGATAATTTAGAGAATAAGAGTACTGTCAAAAAGGTTAAGCTTAAGGTTAATGGTGTTACACGCACAATTCAGGCCAAGTCTACATCTGATGGTGCATCTAATGTCGGTTTGTCTTCTACAAAATCCTCTCATATTTCAGATGCCCCTCGACCACGGAAGAGGTTGATTCTTCAGGAAAATTCAGATGACAATGGTTCCTTTGGTTCAGACAAGGGAAGTGGGTTACGAGGGGTTGCATCTAAGGATCTATCTAAAAGTGTCTTTGATCTTGGGAAGGATGATAGTTCAAGGGGTAGGATGCCTGAAGAACCTATTCGCAAGAGCAAACGCATCCCTAAGAGACGTGTGCTGGATGGAACAGTTGATGTTGGCGATGATGATGATGATGTAGAAGTTCGATACCTTGAAAAACTCAAAACATCTAAGAATACCCCAGATTACAGTTTGGACTACCAAGAGGATGAGGAAAGAGCAGTCAGGAAAGAAAGGAAGATTTCAAGGGTGTTGAAGGGAAGTGGTGGTGGCCAGTGCAGTGTAGATATGGTAGATTATGGCATATCAAGGTCAAGCAAGGAGGGTAAGAAGTCAAGATCGGGAAGAGTAGTTGATGATACTGATTATGTAGAAGAAGACGAACCTGTTTCTGATGGCGAGCCAGAACTCAAGATCAGGAAGAAATTGAGAAAGGAGTTTGTTGAGTCATCCTCAGATAATAAGGAGATGACAGTAACTACTCGTCGAAGGGCCCTTCAAACTAGCAAAGATGTCTCTTCCAGCATAAGTCCAAGCGCAATTGAGTTTCCCAATGGGTTGCCTCCTGCACCACCAAGAAAGCAAAAAGATGAATTCTGTGCAGTGGAGCAGCAACTGAAGAAAGTTGAGGCTGCTCAGAGACGTAGGATGCAAGTAGAGAAGGCAGCTAGAGAATCTGAGGCTGAGGCAATCAGAAAAATTTTGGGCCAAGATTCCAGTCGGAAGAAAAAGGAAGACAAGATAAAGAAACGGCAAGAAGATCTGGCACAGGAGAGAGCTGCAAATGCTATGCTTCCATCAGATTCTGTTCGATGGGTGATGGGTCCATCAGGATCGATTGTTACTTTTCCTGATACAGTTGGTTTACCAACCATATTTGACCCGAAGCCTTGCAGTTACCCTCCTCCCCGTGAGAAGTGCGCCGGACCATATTGTACAAACCCATACAAGTACAGGGATTCCCAATCAAAGCTCCCTCTCTGCAGTCTCCAGTGCTACAAGGCAGTTCACGAAAAGATGCCTTCTCTATCTGCCTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PAPA-1 zf-HIT

Protein Analysis

543

Amino Acids

59.89

Weight (kDa)

9.29

Isoelectric Point (pI)

53.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAPA-1	PF04795	399 - 482	1.2e-21	PAPA-1-like conserved region
zf-HIT	PF04438	499 - 530	4.4e-07	HIT zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56460 AT1G56460 AT1G56460
fragaria_vesca	FvH4_6g07480 FvH4_6g07480 FvH4_6g07480 FvH4_6g07480
malus_domestica	MD00G1208200.v1.1 MD04G1214800.v1.1 MD04G1215000.v1.1 MD12G1205400.v1.1
prunus_persica	Prupe.6G312500_v2.0.a1
pyrus_communis	pycom12g19330
rosa_chinensis	RchiOBHm_Chr3g0457151 RchiOBHm_Chr3g0457181
rosa_laevigata	RLG00000025244
rosa_multiflora	Rmu_sc0000488.1_g000012
rosa_roxburghii	Rroxscaffold_6G00422130
rosa_rugosa	Rorug03G0019200 Rorug03G0019300 Rorug03G0019300 Rorug03G0019400
rosa_samantha	Rh3AG079100 Rh3BG080800 Rh3CG080900 Rh3DG082800
rosa_wichuraiana	Rw3G006800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	416
AciI	CCGC	1 cut(s)	15
AclWI	GGATC	2 cut(s)	588, 1429
AcoI	YGGCCR	1 cut(s)	859
AcsI	RAATTY	3 cut(s)	514, 1181, 1287
AcuI	CTGAAG	4 cut(s)	129, 491, 660, 1226
AfaI	GTAC	3 cut(s)	358, 1525, 1541
AfiI	CCNNNNNNNGG	1 cut(s)	310
AflII	CTTAAG	1 cut(s)	377
AflIII	ACRYGT	1 cut(s)	679
AgsI	TTSAA	7 cut(s)	188, 204, 624, 743, 834, 844, 1088
AjiI	CACGTC	1 cut(s)	680
AluBI	AGCT	6 cut(s)	97, 200, 376, 1256, 1367, 1561
AluI	AGCT	6 cut(s)	97, 200, 376, 1256, 1367, 1561
Alw26I	GTCTC	4 cut(s)	670, 1108, 1222, 1580
AlwI	GGATC	2 cut(s)	588, 1429
AlwNI	CAGNNNCTG	2 cut(s)	1204, 1394
AoxI	GGCC	6 cut(s)	283, 301, 408, 859, 1080, 1294
ApaI	GGGCCC	1 cut(s)	1084
ApeKI	GCWGC	5 cut(s)	44, 1198, 1220, 1253, 1367
ApoI	RAATTY	3 cut(s)	514, 1181, 1287
Asp700I	GAANNNNTTC	1 cut(s)	648
AspLEI	GCGC	2 cut(s)	1127, 1511
AspS9I	GGNCC	7 cut(s)	284, 302, 1080, 1081, 1294, 1412, 1514
AsuHPI	GGTGA	1 cut(s)	1417
AvaII	GGWCC	2 cut(s)	1412, 1514
BaeGI	GKGCMC	1 cut(s)	1084
BalI	TGGCCA	1 cut(s)	861
BanII	GRGCYC	1 cut(s)	1084
BbsI	GAAGAC	3 cut(s)	441, 972, 1329
BbvCI	CCTCAGC	1 cut(s)	1272
BbvI	GCAGC	5 cut(s)	31, 1207, 1210, 1265, 1354
BccI	CCATC	8 cut(s)	308, 419, 683, 977, 1393, 1395, 1402, 1423
BceAI	ACGGC	1 cut(s)	1355
BciVI	GTATCC	1 cut(s)	173
BcoDI	GTCTC	4 cut(s)	670, 1108, 1222, 1580
BfaI	CTAG	2 cut(s)	1092, 1257
BfmI	CTRYAG	1 cut(s)	1570
BfrI	CTTAAG	1 cut(s)	377
BfuI	GTATCC	1 cut(s)	173
BglII	AGATCT	1 cut(s)	1348
BisI	GCNGC	6 cut(s)	16, 45, 1199, 1221, 1254, 1368
BlsI	GCNGC	6 cut(s)	17, 46, 1200, 1222, 1255, 1369
BmcAI	AGTACT	1 cut(s)	358
Bme18I	GGWCC	2 cut(s)	1412, 1514
BmgBI	CACGTC	1 cut(s)	680
BmgT120I	GGNCC	7 cut(s)	284, 302, 1080, 1081, 1294, 1412, 1514
BmiI	GGNNCC	4 cut(s)	304, 532, 1082, 1413
BmrI	ACTGGG	1 cut(s)	255
BmsI	GCATC	7 cut(s)	440, 466, 581, 624, 675, 1227, 1596
BmuI	ACTGGG	1 cut(s)	255
BpiI	GAAGAC	3 cut(s)	441, 972, 1329
BplI	GAGNNNNNCTC	2 cut(s)	1025, 1057
BpmI	CTGGAG	1 cut(s)	1562
Bpu10I	CCTNAGC	1 cut(s)	1272
BpuEI	CTTGAG	1 cut(s)	983
Bsa29I	ATCGAT	1 cut(s)	1424
BsaJI	CCNNGG	3 cut(s)	80, 280, 488
BsaXI	ACNNNNNCTCC	2 cut(s)	168, 198
Bsc4I	CCNNNNNNNGG	1 cut(s)	310
Bse1I	ACTGG	4 cut(s)	250, 862, 1305, 1579
BseCI	ATCGAT	1 cut(s)	1424
BseDI	CCNNGG	3 cut(s)	80, 280, 488
BseGI	GGATG	8 cut(s)	319, 619, 639, 666, 694, 802, 1037, 1242
BseLI	CCNNNNNNNGG	1 cut(s)	310
BseMII	CTCAG	5 cut(s)	159, 1056, 1238, 1257, 1263
BseNI	ACTGG	4 cut(s)	250, 862, 1305, 1579
BseRI	GAGGAG	3 cut(s)	129, 189, 1482
BseSI	GKGCMC	1 cut(s)	1084
BseXI	GCAGC	5 cut(s)	31, 1207, 1210, 1265, 1354
BseYI	CCCAGC	1 cut(s)	305
BsgI	GTGCAG	3 cut(s)	886, 1140, 1209
BshFI	GGCC	6 cut(s)	285, 303, 410, 861, 1082, 1296
BshVI	ATCGAT	1 cut(s)	1424
BsiSI	CCGG	1 cut(s)	1512
BslI	CCNNNNNNNGG	1 cut(s)	310
BsmAI	GTCTC	4 cut(s)	670, 1108, 1222, 1580
BsmBI	CGTCTC	2 cut(s)	670, 1222
BsnI	GGCC	6 cut(s)	285, 303, 410, 861, 1082, 1296
Bsp120I	GGGCCC	1 cut(s)	1080
Bsp1286I	GDGCHC	1 cut(s)	1084
Bsp1407I	TGTACA	1 cut(s)	1523
Bsp143I	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
BspACI	CCGC	1 cut(s)	15
BspANI	GGCC	6 cut(s)	285, 303, 410, 861, 1082, 1296
BspCNI	CTCAG	5 cut(s)	160, 1055, 1237, 1258, 1264
BspDI	ATCGAT	1 cut(s)	1424
BspLI	GGNNCC	4 cut(s)	304, 532, 1082, 1413
BspMAI	CTGCAG	1 cut(s)	1574
BspPI	GGATC	2 cut(s)	588, 1429
BspTI	CTTAAG	1 cut(s)	377
BsrGI	TGTACA	1 cut(s)	1523
BsrI	ACTGG	4 cut(s)	250, 862, 1305, 1579
BssECI	CCNNGG	3 cut(s)	80, 280, 488
BssMI	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
BssT1I	CCWWGG	1 cut(s)	280
Bst4CI	ACNGT	7 cut(s)	41, 129, 361, 697, 779, 1063, 1447
Bst6I	CTCTTC	4 cut(s)	127, 488, 928, 1111
BstAFI	CTTAAG	1 cut(s)	377
BstAUI	TGTACA	1 cut(s)	1523
BstC8I	GCNNGC	1 cut(s)	989
BstDEI	CTNAG	8 cut(s)	168, 576, 672, 759, 1042, 1224, 1266, 1272
BstDSI	CCRYGG	1 cut(s)	488
BstF5I	GGATG	8 cut(s)	319, 619, 639, 666, 694, 802, 1037, 1242
BstHHI	GCGC	2 cut(s)	1127, 1511
BstKTI	GATC	6 cut(s)	583, 604, 929, 1005, 1351, 1424
BstMAI	GTCTC	4 cut(s)	670, 1108, 1222, 1580
BstMBI	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
BstMWI	GCNNNNNNNGC	1 cut(s)	1373
BstSFI	CTRYAG	1 cut(s)	1570
BstSLI	GKGCMC	1 cut(s)	1084
BstV1I	GCAGC	5 cut(s)	31, 1207, 1210, 1265, 1354
BstV2I	GAAGAC	3 cut(s)	441, 972, 1329
BstX2I	RGATCY	2 cut(s)	580, 1348
BstYI	RGATCY	2 cut(s)	580, 1348
Bsu15I	ATCGAT	1 cut(s)	1424
BsuI	GTATCC	1 cut(s)	173
BsuRI	GGCC	6 cut(s)	285, 303, 410, 861, 1082, 1296
BsuTUI	ATCGAT	1 cut(s)	1424
BtgI	CCRYGG	1 cut(s)	488
BtgZI	GCGATG	1 cut(s)	723
BtrI	CACGTC	1 cut(s)	680
BtsCI	GGATG	8 cut(s)	319, 619, 639, 666, 694, 802, 1037, 1242
BtsI	GCAGTG	2 cut(s)	874, 1197
BtsIMutI	CAGTG	7 cut(s)	34, 46, 125, 869, 874, 1197, 1586
Cac8I	GCNNGC	1 cut(s)	989
CaiI	CAGNNNCTG	2 cut(s)	1204, 1394
CfoI	GCGC	2 cut(s)	1127, 1511
Cfr13I	GGNCC	7 cut(s)	284, 302, 1080, 1081, 1294, 1412, 1514
ClaI	ATCGAT	1 cut(s)	1424
Csp6I	GTAC	3 cut(s)	357, 1524, 1540
CspCI	CAANNNNNGTGG	2 cut(s)	535, 570
CviAII	CATG	1 cut(s)	271
CviQI	GTAC	3 cut(s)	357, 1524, 1540
DdeI	CTNAG	8 cut(s)	168, 576, 672, 759, 1042, 1224, 1266, 1272
DpnI	GATC	6 cut(s)	582, 603, 928, 1004, 1350, 1423
DpnII	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
EaeI	YGGCCR	1 cut(s)	859
Eam1104I	CTCTTC	4 cut(s)	127, 488, 928, 1111
EarI	CTCTTC	4 cut(s)	127, 488, 928, 1111
Eco130I	CCWWGG	1 cut(s)	280
Eco24I	GRGCYC	1 cut(s)	1084
Eco47I	GGWCC	2 cut(s)	1412, 1514
Eco57I	CTGAAG	4 cut(s)	129, 491, 660, 1226
EcoO109I	RGGNCCY	3 cut(s)	302, 1080, 1081
EcoRI	GAATTC	1 cut(s)	1181
EcoT14I	CCWWGG	1 cut(s)	280
EcoT38I	GRGCYC	1 cut(s)	1084
ErhI	CCWWGG	1 cut(s)	280
Esp3I	CGTCTC	2 cut(s)	670, 1222
FaeI	CATG	1 cut(s)	274
FatI	CATG	1 cut(s)	270
FblI	GTMKAC	1 cut(s)	416
Fnu4HI	GCNGC	6 cut(s)	16, 45, 1199, 1221, 1254, 1368
FokI	GGATG	8 cut(s)	326, 626, 646, 653, 701, 809, 1024, 1249
FriOI	GRGCYC	1 cut(s)	1084
Fsp4HI	GCNGC	6 cut(s)	16, 45, 1199, 1221, 1254, 1368
FspBI	CTAG	2 cut(s)	1092, 1257
GlaI	GCGC	2 cut(s)	1126, 1510
GluI	GCNGC	6 cut(s)	16, 45, 1199, 1221, 1254, 1368
GsaI	CCCAGC	1 cut(s)	309
GsuI	CTGGAG	1 cut(s)	1562
HaeIII	GGCC	6 cut(s)	285, 303, 410, 861, 1082, 1296
HapII	CCGG	1 cut(s)	1512
HhaI	GCGC	2 cut(s)	1127, 1511
Hin1II	CATG	1 cut(s)	274
Hin6I	GCGC	2 cut(s)	1125, 1509
HinP1I	GCGC	2 cut(s)	1125, 1509
HindIII	AAGCTT	2 cut(s)	198, 374
HpaII	CCGG	1 cut(s)	1512
HphI	GGTGA	1 cut(s)	1417
Hpy166II	GTNNAC	4 cut(s)	37, 417, 1454, 1597
Hpy8I	GTNNAC	4 cut(s)	37, 417, 1454, 1597
Hpy99I	CGWCG	2 cut(s)	78, 1077
HpyAV	CCTTC	7 cut(s)	153, 604, 838, 1071, 1094, 1243, 1620
HpyCH4III	ACNGT	7 cut(s)	41, 129, 361, 697, 779, 1063, 1447
HpyCH4IV	ACGT	3 cut(s)	183, 679, 1231
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1373
HpyF3I	CTNAG	8 cut(s)	168, 576, 672, 759, 1042, 1224, 1266, 1272
HpySE526I	ACGT	3 cut(s)	183, 679, 1231
Hsp92II	CATG	1 cut(s)	274
HspAI	GCGC	2 cut(s)	1125, 1509
Kzo9I	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
LmnI	GCTCC	3 cut(s)	60, 1195, 1566
Lsp1109I	GCAGC	5 cut(s)	31, 1207, 1210, 1265, 1354
LweI	GCATC	7 cut(s)	440, 466, 581, 624, 675, 1227, 1596
MaeI	CTAG	2 cut(s)	1092, 1257
MaeII	ACGT	3 cut(s)	183, 679, 1231
MaeIII	GTNAC	7 cut(s)	90, 113, 391, 558, 1063, 1429, 1484
MalI	GATC	6 cut(s)	582, 603, 928, 1004, 1350, 1423
MboI	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
MfeI	CAATTG	1 cut(s)	1128
MflI	RGATCY	2 cut(s)	580, 1348
MhlI	GDGCHC	1 cut(s)	1084
MlsI	TGGCCA	1 cut(s)	861
MluCI	AATT	7 cut(s)	340, 402, 514, 1013, 1128, 1181, 1287
MluNI	TGGCCA	1 cut(s)	861
MlyI	GAGTC	2 cut(s)	98, 1043
MmeI	TCCRAC	1 cut(s)	1289
Mox20I	TGGCCA	1 cut(s)	861
MroXI	GAANNNNTTC	1 cut(s)	648
MscI	TGGCCA	1 cut(s)	861
MseI	TTAA	5 cut(s)	162, 372, 378, 384, 1627
Msp20I	TGGCCA	1 cut(s)	861
MspA1I	CMGCKG	1 cut(s)	15
MspCI	CTTAAG	1 cut(s)	377
MspI	CCGG	1 cut(s)	1512
MunI	CAATTG	1 cut(s)	1128
MwoI	GCNNNNNNNGC	1 cut(s)	1373
NdeII	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
NlaIII	CATG	1 cut(s)	274
NlaIV	GGNNCC	4 cut(s)	304, 532, 1082, 1413
NmuCI	GTSAC	2 cut(s)	90, 113
PdmI	GAANNNNTTC	1 cut(s)	648
PfeI	GAWTC	8 cut(s)	24, 188, 254, 502, 1262, 1301, 1391, 1547
PkrI	GCNGC	6 cut(s)	17, 46, 1200, 1222, 1255, 1369
PleI	GAGTC	2 cut(s)	97, 1042
PpsI	GAGTC	2 cut(s)	97, 1042
PspFI	CCCAGC	1 cut(s)	305
PspN4I	GGNNCC	4 cut(s)	304, 532, 1082, 1413
PspOMI	GGGCCC	1 cut(s)	1080
PspPI	GGNCC	7 cut(s)	284, 302, 1080, 1081, 1294, 1412, 1514
PstI	CTGCAG	1 cut(s)	1574
PstNI	CAGNNNCTG	2 cut(s)	1204, 1394
PsuI	RGATCY	2 cut(s)	580, 1348
RsaI	GTAC	3 cut(s)	358, 1525, 1541
RsaNI	GTAC	3 cut(s)	357, 1524, 1540
SaqAI	TTAA	5 cut(s)	162, 372, 378, 384, 1627
SatI	GCNGC	6 cut(s)	16, 45, 1199, 1221, 1254, 1368
Sau3AI	GATC	6 cut(s)	580, 601, 926, 1002, 1348, 1421
Sau96I	GGNCC	7 cut(s)	284, 302, 1080, 1081, 1294, 1412, 1514
ScaI	AGTACT	1 cut(s)	358
SchI	GAGTC	2 cut(s)	98, 1043
SduI	GDGCHC	1 cut(s)	1084
SfaNI	GCATC	7 cut(s)	440, 466, 581, 624, 675, 1227, 1596
SfcI	CTRYAG	1 cut(s)	1570
SinI	GGWCC	2 cut(s)	1412, 1514
SmlI	CTYRAG	2 cut(s)	377, 998
SmoI	CTYRAG	2 cut(s)	377, 998
Sse9I	AATT	7 cut(s)	340, 402, 514, 1013, 1128, 1181, 1287
SsiI	CCGC	1 cut(s)	15
SspMI	CTAG	2 cut(s)	1092, 1257
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	7 cut(s)	41, 129, 361, 697, 779, 1063, 1447
TaiI	ACGT	3 cut(s)	186, 682, 1234
TaqI	TCGA	6 cut(s)	220, 484, 733, 1075, 1399, 1424
TasI	AATT	7 cut(s)	340, 402, 514, 1013, 1128, 1181, 1287
TatI	WGTACW	3 cut(s)	356, 1523, 1539
TauI	GCSGC	1 cut(s)	18
TfiI	GAWTC	8 cut(s)	24, 188, 254, 502, 1262, 1301, 1391, 1547
Tru1I	TTAA	5 cut(s)	162, 372, 378, 384, 1627
Tru9I	TTAA	5 cut(s)	162, 372, 378, 384, 1627
TscAI	CASTG	7 cut(s)	34, 46, 132, 869, 874, 1197, 1586
TseFI	GTSAC	2 cut(s)	90, 113
TseI	GCWGC	5 cut(s)	44, 1198, 1220, 1253, 1367
Tsp45I	GTSAC	2 cut(s)	90, 113
TspDTI	ATGAA	2 cut(s)	210, 1194
TspGWI	ACGGA	1 cut(s)	505
TspRI	CASTG	7 cut(s)	34, 46, 132, 869, 874, 1197, 1586
Vha464I	CTTAAG	1 cut(s)	377
VpaK11BI	GGWCC	2 cut(s)	1412, 1514
XapI	RAATTY	3 cut(s)	514, 1181, 1287
XmiI	GTMKAC	1 cut(s)	416
XmnI	GAANNNNTTC	1 cut(s)	648
XspI	CTAG	2 cut(s)	1092, 1257
ZrmI	AGTACT	1 cut(s)	358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.