Rroxscaffold_6G00422130

PAPA-1-like conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005975
Physical Location & Seq
Forward (+)
43123306 .. 43126843
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422130.1

Sequence Viewer

Length: 849 bp
ATGGAGAGTTTCGGCGGCTTGGGATTCAGTGAAGTAAACAGTGCTGCAAGGAGGAAAAGGAGCAATACATCCCGTCGTCCTCGGAATGAGTCACAGCTTCCTTTGGATTATCGTGACATCTCTTCACTGTCATCAACTCCTCCTTCAGACGATAATATGATTAAATCTGAGGGAGGAGGATACGTTGAATCTGATGAAGCATCAAATAATGGTTCTTTTCGTGGCAGTAATGAGCATAGGCATACTGGCATTGATTCCAAAAGGTCGAGCATGGGTGTCCTTGGCCCTGCCAATAGGAAAGGCCCAAGCAAGGATGGGTCTTTTGGTATTGTTTCAGATAATTTAGAGAATAAGAGTACTGTGAAAAAGGTTAAGCTTAAGGTTGGTGGTGTTACACGCACAATTCAGGCCAAGTCTACATCTGATGGTGCATCTAATGTCGGGTTGTCTTCTACAAAATCTTCTCATATTTCAGATGCCCCTCGACCACGGAAGAGGTTGATTCTTCAGGAAAATTCAGATGACAATGGTTCCTTTGGTTCAGACAAGGGAAGTGGGTTACGGGGGTTGCATCTAAGGATCCATCTAAAAGTGTCTTTGATCTTGGGAAGGATGATAGTTCAAGGGGTAGGATTCCTGAAGAACCTATTCGCAAGAGCAAACGCATCCCTAAGAGACGTGTGCTGGATGGAACAGTTGATGTTGGCGATGATGATGATGATGATGATGTGGAAGTTCGATACCTTGAAAAACTCAAAACATTTAAGAACACCCCAGATTACAGTTTGGACTACCAAGAGGATGAGGAAAGAGCAGTCAGGAAAGAAAGGAAGATTTCAAGGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAPA-1 zf-HIT

Protein Analysis

282

Amino Acids

31.04

Weight (kDa)

10.37

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 416
AciI CCGC 1 cut(s) 15
AclWI GGATC 2 cut(s) 574, 587
AcsI RAATTY 1 cut(s) 514
AcuI CTGAAG 3 cut(s) 129, 491, 659
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 1 cut(s) 310
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 4 cut(s) 188, 623, 748, 839
AjiI CACGTC 1 cut(s) 679
AluBI AGCT 2 cut(s) 97, 376
AluI AGCT 2 cut(s) 97, 376
Alw26I GTCTC 1 cut(s) 669
AlwI GGATC 2 cut(s) 574, 587
AoxI GGCC 3 cut(s) 283, 301, 408
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 514
Asp700I GAANNNNTTC 1 cut(s) 647
AspS9I GGNCC 2 cut(s) 284, 302
BamHI GGATCC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 31
BccI CCATC 4 cut(s) 308, 419, 591, 682
BciVI GTATCC 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 669
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 173
BisI GCNGC 2 cut(s) 16, 45
BlsI GCNGC 2 cut(s) 17, 46
BmcAI AGTACT 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 2 cut(s) 284, 302
BmiI GGNNCC 2 cut(s) 532, 581
BmsI GCATC 5 cut(s) 209, 440, 466, 580, 674
BpiI GAAGAC 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 80, 280, 488
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 250
BseDI CCNNGG 3 cut(s) 80, 280, 488
BseGI GGATG 6 cut(s) 68, 319, 618, 665, 693, 807
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 129, 189
BseXI GCAGC 1 cut(s) 31
BshFI GGCC 3 cut(s) 285, 303, 410
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 1 cut(s) 669
BsmBI CGTCTC 1 cut(s) 669
BsnI GGCC 3 cut(s) 285, 303, 410
Bsp143I GATC 2 cut(s) 579, 600
BspACI CCGC 1 cut(s) 15
BspANI GGCC 3 cut(s) 285, 303, 410
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 532, 581
BspPI GGATC 2 cut(s) 574, 587
BspTI CTTAAG 1 cut(s) 377
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 80, 280, 488
BssMI GATC 2 cut(s) 579, 600
BssT1I CCWWGG 1 cut(s) 280
Bst4CI ACNGT 5 cut(s) 41, 129, 361, 696, 784
Bst6I CTCTTC 2 cut(s) 127, 488
BstAFI CTTAAG 1 cut(s) 377
BstDEI CTNAG 3 cut(s) 168, 575, 671
BstDSI CCRYGG 1 cut(s) 488
BstF5I GGATG 6 cut(s) 68, 319, 618, 665, 693, 807
BstKTI GATC 2 cut(s) 582, 603
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 2 cut(s) 579, 600
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 3 cut(s) 285, 303, 410
BtgI CCRYGG 1 cut(s) 488
BtgZI GCGATG 1 cut(s) 722
BtrI CACGTC 1 cut(s) 679
BtsCI GGATG 6 cut(s) 68, 319, 618, 665, 693, 807
BtsIMutI CAGTG 3 cut(s) 34, 46, 125
Cfr13I GGNCC 2 cut(s) 284, 302
Csp6I GTAC 1 cut(s) 357
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 1 cut(s) 271
CviJI RGCY 6 cut(s) 18, 97, 285, 303, 376, 410
CviKI_1 RGCY 6 cut(s) 18, 97, 285, 303, 376, 410
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 3 cut(s) 168, 575, 671
DpnI GATC 2 cut(s) 581, 602
DpnII GATC 2 cut(s) 579, 600
Eam1104I CTCTTC 2 cut(s) 127, 488
EarI CTCTTC 2 cut(s) 127, 488
Eco130I CCWWGG 1 cut(s) 280
Eco57I CTGAAG 3 cut(s) 129, 491, 659
EcoT14I CCWWGG 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 280
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 1 cut(s) 274
FaiI YATR 5 cut(s) 158, 237, 243, 272, 468
FatI CATG 1 cut(s) 270
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 2 cut(s) 16, 45
FokI GGATG 6 cut(s) 55, 326, 625, 652, 700, 814
Fsp4HI GCNGC 2 cut(s) 16, 45
GluI GCNGC 2 cut(s) 16, 45
HaeIII GGCC 3 cut(s) 285, 303, 410
Hin1II CATG 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 6 cut(s) 24, 89, 188, 254, 502, 633
Hpy166II GTNNAC 2 cut(s) 37, 417
Hpy188I TCNGA 9 cut(s) 84, 148, 169, 193, 337, 424, 475, 520, 544
Hpy188III TCNNGA 4 cut(s) 113, 509, 637, 819
Hpy8I GTNNAC 2 cut(s) 37, 417
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 2 cut(s) 153, 603
HpyCH4III ACNGT 5 cut(s) 41, 129, 361, 696, 784
HpyCH4IV ACGT 2 cut(s) 183, 678
HpyCH4V TGCA 3 cut(s) 47, 431, 571
HpyF3I CTNAG 3 cut(s) 168, 575, 671
HpySE526I ACGT 2 cut(s) 183, 678
Hsp92II CATG 1 cut(s) 274
Kzo9I GATC 2 cut(s) 579, 600
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 8 cut(s) 231, 300, 392, 494, 650, 670, 788, 804
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 5 cut(s) 209, 440, 466, 580, 674
MaeII ACGT 2 cut(s) 183, 678
MaeIII GTNAC 4 cut(s) 90, 113, 391, 558
MalI GATC 2 cut(s) 581, 602
MboI GATC 2 cut(s) 579, 600
MboII GAAGA 7 cut(s) 114, 441, 453, 497, 505, 652, 843
MflI RGATCY 1 cut(s) 579
MluCI AATT 3 cut(s) 340, 402, 514
MlyI GAGTC 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 4 cut(s) 162, 372, 378, 764
MspCI CTTAAG 1 cut(s) 377
NdeII GATC 2 cut(s) 579, 600
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 2 cut(s) 532, 581
NmuCI GTSAC 2 cut(s) 90, 113
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 5 cut(s) 24, 188, 254, 502, 633
PkrI GCNGC 2 cut(s) 17, 46
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PspN4I GGNNCC 2 cut(s) 532, 581
PspPI GGNCC 2 cut(s) 284, 302
PsuI RGATCY 1 cut(s) 579
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
SaqAI TTAA 4 cut(s) 162, 372, 378, 764
SatI GCNGC 2 cut(s) 16, 45
Sau3AI GATC 2 cut(s) 579, 600
Sau96I GGNCC 2 cut(s) 284, 302
ScaI AGTACT 1 cut(s) 358
SchI GAGTC 1 cut(s) 98
SfaNI GCATC 5 cut(s) 209, 440, 466, 580, 674
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 3 cut(s) 340, 402, 514
SsiI CCGC 1 cut(s) 15
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 5 cut(s) 41, 129, 361, 696, 784
TaiI ACGT 2 cut(s) 186, 681
TaqI TCGA 3 cut(s) 266, 484, 738
TasI AATT 3 cut(s) 340, 402, 514
TatI WGTACW 1 cut(s) 356
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 5 cut(s) 24, 188, 254, 502, 633
Tru1I TTAA 4 cut(s) 162, 372, 378, 764
Tru9I TTAA 4 cut(s) 162, 372, 378, 764
TscAI CASTG 3 cut(s) 34, 46, 132
TseFI GTSAC 2 cut(s) 90, 113
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 2 cut(s) 90, 113
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 3 cut(s) 34, 46, 132
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 416
XmnI GAANNNNTTC 1 cut(s) 647
ZrmI AGTACT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.