Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010576

Physical Location & Seq

Genomic Coordinates Forward (+)

31881107 .. 31882867

Gene Structure 1761 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1249400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1260 bp

ATGGGGTGCTTTCCAAAGTGCTTCTCTACTTGTAAGCGCCGAAAGCTCAAAAAATCAGTCACTGTAAACCCCTTTAAACACCAAAGCATTGAAGTTGCTGAAGAAGCTGTTCAAATCCAACCTGCTGAAGCTGAACCCACAAAGCTAGAAGAATTTATAGAAGCCATTAAACCCAGCAAAGAATCAGTAGAGGAGATTGAAGAACAATCAAAGAATGGTGACAAAAAGAAAGTCACATTTGATTTGAATGTCGAGGCAAATGATGAAGAAACACCCACCAAGGAAGAAGTCTCCAATGTACTGGTGGAAATTTATGATGAGATGGAGAATGAAGAGGAAGCGCGAAAAGGGTTCGAACCGGATTTGATTGTATCAAGTATGGTATCACCATCAACTCATCCGTTGAATCATCGACACAAAAATTGCGAAAACAGTGAGGATGAAGATGATGATTGTGCTAATGCTGATGTGTTTGAAAGTAGCAAGCAAATCTTTGTTGAAGAAGAGTCTTCCGAGTCCTTGTTTTCGCTATCAATTGATTCTCGAAAACAAGAAGTTTGTGATGATGCTGGAACAGATGAGAAGGAGGTGAATAGTCGAATGCCAAATCATCAAAATGGCAGAATAGATTACAGGAGACAGAGATTTCACCTGCAGGCGGTATCGAACCCCATTGAAAATCTTGCTCAATGGAAGGAGGAGGTCACCGGAAAGTCAACTCCAACAGAAGCTTCAAAACAGCAGCACCAAGAGAAGGAGAACATAAACATGGAGGTGGAGGAGCAGGATTTGAAATTACACTTTAGTCCGGAGCCTGCTTCGAAGCTGCCAAAACTCTCCGCAAGGCCGAGGACAAAGTCGTCTTCTTGCGTAGACACGAGCCTTTCGAGCTGGCTGGTTGAGTCTGAGACCACACCACAGTCAAATGCAAGTACTAATAATTCTGTGGGGAACGAGTTTAAGTCACCCGGAAGCCGTGAAGATAGGCCAATTCTGGGAGCTTGGACAACTGAAGAGCTAAAGCAGTTGTCGGCTTCTTCTACACCGAAAAAAGGGTTACGAAGCCCGAGTCCCGAGGAAATGCCGATCATTGGTACTGTTGGGAGCTACTGGAGTCGCACCGGACAGGCCATGGATTCGGACTCAGGCTCTTCTTGCAGAGGAAGGCCAAAATCAACAGGGCAACATAGACAGGATCAGAAAGTGGAATGGAAGTCCACTCCATTTAAGGCAAAATTGGAGAGAGCTTTGGAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

420

Amino Acids

47.19

Weight (kDa)

5.03

Isoelectric Point (pI)

64.23

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010576)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g01750
malus_domestica	MD04G1231100.v1.1 MD12G1249400.v1.1
prunus_persica	Prupe.6G350800_v2.0.a1 Prupe.6G350800_v2.0.a1
pyrus_communis	pycom04g20470 pycom12g22920
rosa_chinensis	RchiOBHm_Chr3g0449491
rosa_laevigata	RLG00000025822 RLG00000025825
rosa_multiflora	Rmu_sc0000430.1_g000018 Rmu_sc0019012.1_g000001
rosa_roxburghii	Rroxscaffold_6G00427450
rosa_rugosa	Rorug02G0621600 Rorug02G0621700
rosa_samantha	Rh3AG019800 Rh3BG019800 Rh3CG018500 Rh3DG019800
rosa_wichuraiana	Rw3G001540 Rw3G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	660
AasI	GACNNNNNNGTC	1 cut(s)	859
Acc36I	ACCTGC	2 cut(s)	130, 660
AccI	GTMKAC	1 cut(s)	873
AccII	CGCG	1 cut(s)	343
AccIII	TCCGGA	1 cut(s)	808
AciI	CCGC	2 cut(s)	659, 840
AclWI	GGATC	1 cut(s)	1203
AcsI	RAATTY	2 cut(s)	152, 309
AcuI	CTGAAG	3 cut(s)	120, 147, 1032
AfaI	GTAC	3 cut(s)	300, 934, 1096
AfiI	CCNNNNNNNGG	5 cut(s)	658, 754, 995, 1052, 1091
AjuI	GAANNNNNNNTTGG	2 cut(s)	715, 747
Alw26I	GTCTC	3 cut(s)	295, 631, 902
AlwI	GGATC	1 cut(s)	1203
AlwNI	CAGNNNCTG	1 cut(s)	62
Ama87I	CYCGRG	2 cut(s)	1066, 1073
Aor13HI	TCCGGA	1 cut(s)	808
AoxI	GGCC	4 cut(s)	845, 986, 1128, 1166
ApeKI	GCWGC	2 cut(s)	742, 826
ApoI	RAATTY	2 cut(s)	152, 309
ArsI	GACNNNNNNTTYG	6 cut(s)	42, 74, 216, 248, 1054, 1086
Asp700I	GAANNNNTTC	1 cut(s)	108
AspLEI	GCGC	2 cut(s)	39, 343
AsuC2I	CCSGG	1 cut(s)	969
AsuHPI	GGTGA	6 cut(s)	230, 378, 601, 641, 697, 957
AsuII	TTCGAA	2 cut(s)	354, 821
AvaI	CYCGRG	2 cut(s)	1066, 1073
BauI	CACGAG	1 cut(s)	877
BbsI	GAAGAC	2 cut(s)	501, 855
BbvI	GCAGC	2 cut(s)	754, 813
BccI	CCATC	2 cut(s)	316, 397
BceAI	ACGGC	1 cut(s)	960
BcnI	CCSGG	1 cut(s)	969
BcoDI	GTCTC	3 cut(s)	295, 631, 902
BfaI	CTAG	1 cut(s)	146
BfmI	CTRYAG	1 cut(s)	653
BfoI	RGCGCY	1 cut(s)	40
BfuAI	ACCTGC	2 cut(s)	130, 660
BisI	GCNGC	2 cut(s)	743, 827
BlsI	GCNGC	2 cut(s)	744, 828
BmcAI	AGTACT	1 cut(s)	934
Bme1390I	CCNGG	1 cut(s)	969
BmeT110I	CYCGRG	2 cut(s)	1066, 1073
BmiI	GGNNCC	1 cut(s)	813
BmrFI	CCNGG	1 cut(s)	969
BmsI	GCATC	1 cut(s)	556
BpiI	GAAGAC	2 cut(s)	501, 855
BpmI	CTGGAG	1 cut(s)	1132
Bpu14I	TTCGAA	2 cut(s)	354, 821
BpuMI	CCSGG	1 cut(s)	969
BsaI	GGTCTC	1 cut(s)	902
BsaJI	CCNNGG	4 cut(s)	279, 848, 1074, 1131
BsaWI	WCCGGW	4 cut(s)	358, 707, 808, 1121
Bsc4I	CCNNNNNNNGG	5 cut(s)	658, 754, 995, 1052, 1091
Bse1I	ACTGG	2 cut(s)	306, 1115
BseAI	TCCGGA	1 cut(s)	808
BseDI	CCNNGG	4 cut(s)	279, 848, 1074, 1131
BseGI	GGATG	2 cut(s)	397, 445
BseLI	CCNNNNNNNGG	5 cut(s)	658, 754, 995, 1052, 1091
BseMII	CTCAG	2 cut(s)	897, 1158
BseNI	ACTGG	2 cut(s)	306, 1115
BseRI	GAGGAG	3 cut(s)	206, 713, 794
BseXI	GCAGC	2 cut(s)	754, 813
BseYI	CCCAGC	1 cut(s)	173
Bsh1236I	CGCG	1 cut(s)	343
BshFI	GGCC	4 cut(s)	847, 988, 1130, 1168
BsiHKCI	CYCGRG	2 cut(s)	1066, 1073
BsiSI	CCGG	5 cut(s)	359, 708, 809, 969, 1122
BslFI	GGGAC	1 cut(s)	1056
BslI	CCNNNNNNNGG	5 cut(s)	658, 754, 995, 1052, 1091
BsmAI	GTCTC	3 cut(s)	295, 631, 902
BsmFI	GGGAC	1 cut(s)	1056
BsmI	GAATGC	1 cut(s)	606
BsnI	GGCC	4 cut(s)	847, 988, 1130, 1168
Bso31I	GGTCTC	1 cut(s)	902
BsoBI	CYCGRG	2 cut(s)	1066, 1073
Bsp119I	TTCGAA	2 cut(s)	354, 821
Bsp13I	TCCGGA	1 cut(s)	808
Bsp143I	GATC	2 cut(s)	1086, 1195
Bsp19I	CCATGG	1 cut(s)	1131
BspACI	CCGC	2 cut(s)	659, 840
BspANI	GGCC	4 cut(s)	847, 988, 1130, 1168
BspCNI	CTCAG	2 cut(s)	898, 1157
BspEI	TCCGGA	1 cut(s)	808
BspFNI	CGCG	1 cut(s)	343
BspLI	GGNNCC	1 cut(s)	813
BspMAI	CTGCAG	1 cut(s)	657
BspMI	ACCTGC	2 cut(s)	130, 660
BspPI	GGATC	1 cut(s)	1203
BspQI	GCTCTTC	2 cut(s)	1008, 1156
BspT104I	TTCGAA	2 cut(s)	354, 821
BspTNI	GGTCTC	1 cut(s)	902
BsrI	ACTGG	2 cut(s)	306, 1115
BssECI	CCNNGG	4 cut(s)	279, 848, 1074, 1131
BssMI	GATC	2 cut(s)	1086, 1195
BssSI	CACGAG	1 cut(s)	877
BssT1I	CCWWGG	2 cut(s)	279, 1131
Bst2BI	CACGAG	1 cut(s)	877
Bst4CI	ACNGT	4 cut(s)	64, 434, 921, 1099
Bst6I	CTCTTC	4 cut(s)	327, 498, 1008, 1156
BstBI	TTCGAA	2 cut(s)	354, 821
BstC8I	GCNNGC	4 cut(s)	485, 657, 816, 893
BstDEI	CTNAG	2 cut(s)	906, 1144
BstDSI	CCRYGG	1 cut(s)	1131
BstEII	GGTNACC	1 cut(s)	703
BstF5I	GGATG	2 cut(s)	397, 445
BstFNI	CGCG	1 cut(s)	343
BstH2I	RGCGCY	1 cut(s)	40
BstHHI	GCGC	2 cut(s)	39, 343
BstKTI	GATC	2 cut(s)	1089, 1198
BstMAI	GTCTC	3 cut(s)	295, 631, 902
BstMBI	GATC	2 cut(s)	1086, 1195
BstMWI	GCNNNNNNNGC	4 cut(s)	43, 104, 888, 1155
BstPI	GGTNACC	1 cut(s)	703
BstSCI	CCNGG	1 cut(s)	967
BstSFI	CTRYAG	1 cut(s)	653
BstUI	CGCG	1 cut(s)	343
BstV1I	GCAGC	2 cut(s)	754, 813
BstV2I	GAAGAC	2 cut(s)	501, 855
BstXI	CCANNNNNNTGG	1 cut(s)	301
BsuRI	GGCC	4 cut(s)	847, 988, 1130, 1168
BtgI	CCRYGG	1 cut(s)	1131
BtsCI	GGATG	2 cut(s)	397, 445
BtsIMutI	CAGTG	2 cut(s)	60, 439
BveI	ACCTGC	2 cut(s)	130, 660
Cac8I	GCNNGC	4 cut(s)	485, 657, 816, 893
CaiI	CAGNNNCTG	1 cut(s)	62
CfoI	GCGC	2 cut(s)	39, 343
Csp6I	GTAC	3 cut(s)	299, 933, 1095
CviAII	CATG	2 cut(s)	769, 1132
CviQI	GTAC	3 cut(s)	299, 933, 1095
DdeI	CTNAG	2 cut(s)	906, 1144
DpnI	GATC	2 cut(s)	1088, 1197
DpnII	GATC	2 cut(s)	1086, 1195
DraI	TTTAAA	1 cut(s)	76
DrdI	GACNNNNNNGTC	1 cut(s)	859
DseDI	GACNNNNNNGTC	1 cut(s)	859
Eam1104I	CTCTTC	4 cut(s)	327, 498, 1008, 1156
EarI	CTCTTC	4 cut(s)	327, 498, 1008, 1156
Eco130I	CCWWGG	2 cut(s)	279, 1131
Eco31I	GGTCTC	1 cut(s)	902
Eco57I	CTGAAG	3 cut(s)	120, 147, 1032
Eco88I	CYCGRG	2 cut(s)	1066, 1073
Eco91I	GGTNACC	1 cut(s)	703
EcoO65I	GGTNACC	1 cut(s)	703
EcoT14I	CCWWGG	2 cut(s)	279, 1131
ErhI	CCWWGG	2 cut(s)	279, 1131
FaeI	CATG	2 cut(s)	772, 1135
FaiI	YATR	7 cut(s)	158, 315, 380, 764, 770, 1133, 1188
FalI	AAGNNNNNCTT	2 cut(s)	476, 508
FaqI	GGGAC	1 cut(s)	1056
FatI	CATG	2 cut(s)	768, 1131
FblI	GTMKAC	1 cut(s)	873
Fnu4HI	GCNGC	2 cut(s)	743, 827
FokI	GGATG	2 cut(s)	384, 452
Fsp4HI	GCNGC	2 cut(s)	743, 827
FspBI	CTAG	1 cut(s)	146
GlaI	GCGC	2 cut(s)	38, 342
GluI	GCNGC	2 cut(s)	743, 827
GsaI	CCCAGC	1 cut(s)	177
GsuI	CTGGAG	1 cut(s)	1132
HaeII	RGCGCY	1 cut(s)	40
HaeIII	GGCC	4 cut(s)	847, 988, 1130, 1168
HapII	CCGG	5 cut(s)	359, 708, 809, 969, 1122
HhaI	GCGC	2 cut(s)	39, 343
Hin1II	CATG	2 cut(s)	772, 1135
Hin6I	GCGC	2 cut(s)	37, 341
HinP1I	GCGC	2 cut(s)	37, 341
HincII	GTYRAC	1 cut(s)	717
HindII	GTYRAC	1 cut(s)	717
HindIII	AAGCTT	1 cut(s)	729
HpaII	CCGG	5 cut(s)	359, 708, 809, 969, 1122
HphI	GGTGA	6 cut(s)	230, 378, 601, 641, 697, 957
Hpy166II	GTNNAC	4 cut(s)	67, 717, 874, 1218
Hpy188I	TCNGA	4 cut(s)	514, 907, 1141, 1200
Hpy188III	TCNNGA	3 cut(s)	543, 809, 1073
Hpy8I	GTNNAC	4 cut(s)	67, 717, 874, 1218
HpyAV	CCTTC	4 cut(s)	577, 688, 748, 1158
HpyCH4III	ACNGT	4 cut(s)	64, 434, 921, 1099
HpyCH4V	TGCA	3 cut(s)	655, 929, 1158
HpyF10VI	GCNNNNNNNGC	4 cut(s)	43, 104, 888, 1155
HpyF3I	CTNAG	2 cut(s)	906, 1144
Hsp92II	CATG	2 cut(s)	772, 1135
HspAI	GCGC	2 cut(s)	37, 341
Kpn2I	TCCGGA	1 cut(s)	808
Kzo9I	GATC	2 cut(s)	1086, 1195
LguI	GCTCTTC	2 cut(s)	1008, 1156
LmnI	GCTCC	4 cut(s)	781, 811, 998, 1104
Lsp1109I	GCAGC	2 cut(s)	754, 813
LweI	GCATC	1 cut(s)	556
MaeI	CTAG	1 cut(s)	146
MaeIII	GTNAC	6 cut(s)	58, 218, 232, 703, 963, 1056
MalI	GATC	2 cut(s)	1088, 1197
MboI	GATC	2 cut(s)	1086, 1195
MfeI	CAATTG	1 cut(s)	534
MluCI	AATT	8 cut(s)	152, 309, 421, 534, 794, 940, 990, 1235
MlyI	GAGTC	6 cut(s)	515, 524, 911, 1078, 1123, 1136
MmeI	TCCRAC	2 cut(s)	142, 746
MroI	TCCGGA	1 cut(s)	808
MroXI	GAANNNNTTC	1 cut(s)	108
MseI	TTAA	4 cut(s)	75, 168, 960, 1227
MslI	CAYNNNNRTG	3 cut(s)	615, 767, 773
MspI	CCGG	5 cut(s)	359, 708, 809, 969, 1122
MspR9I	CCNGG	1 cut(s)	969
MunI	CAATTG	1 cut(s)	534
Mva1269I	GAATGC	1 cut(s)	606
MvnI	CGCG	1 cut(s)	343
MwoI	GCNNNNNNNGC	4 cut(s)	43, 104, 888, 1155
NciI	CCSGG	1 cut(s)	969
NcoI	CCATGG	1 cut(s)	1131
NdeII	GATC	2 cut(s)	1086, 1195
NlaIII	CATG	2 cut(s)	772, 1135
NlaIV	GGNNCC	1 cut(s)	813
NmeAIII	GCCGAG	1 cut(s)	873
NmuCI	GTSAC	5 cut(s)	58, 218, 232, 703, 963
NspV	TTCGAA	2 cut(s)	354, 821
PaqCI	CACCTGC	1 cut(s)	660
PciSI	GCTCTTC	2 cut(s)	1008, 1156
PcsI	WCGNNNNNNNCGW	1 cut(s)	884
PctI	GAATGC	1 cut(s)	606
PdmI	GAANNNNTTC	1 cut(s)	108
PfeI	GAWTC	4 cut(s)	182, 406, 539, 1136
PflFI	GACNNNGTC	1 cut(s)	856
PkrI	GCNGC	2 cut(s)	744, 828
PleI	GAGTC	6 cut(s)	514, 523, 910, 1077, 1122, 1136
PpsI	GAGTC	6 cut(s)	514, 523, 910, 1077, 1122, 1136
PspEI	GGTNACC	1 cut(s)	703
PspFI	CCCAGC	1 cut(s)	173
PspN4I	GGNNCC	1 cut(s)	813
PstI	CTGCAG	1 cut(s)	657
PstNI	CAGNNNCTG	1 cut(s)	62
PsyI	GACNNNGTC	1 cut(s)	856
RsaI	GTAC	3 cut(s)	300, 934, 1096
RsaNI	GTAC	3 cut(s)	299, 933, 1095
RseI	CAYNNNNRTG	3 cut(s)	615, 767, 773
SapI	GCTCTTC	2 cut(s)	1008, 1156
SaqAI	TTAA	4 cut(s)	75, 168, 960, 1227
SatI	GCNGC	2 cut(s)	743, 827
Sau3AI	GATC	2 cut(s)	1086, 1195
SbfI	CCTGCAGG	1 cut(s)	657
ScaI	AGTACT	1 cut(s)	934
SchI	GAGTC	6 cut(s)	515, 524, 911, 1078, 1123, 1136
ScrFI	CCNGG	1 cut(s)	969
SdaI	CCTGCAGG	1 cut(s)	657
SfaNI	GCATC	1 cut(s)	556
SfcI	CTRYAG	1 cut(s)	653
SfuI	TTCGAA	2 cut(s)	354, 821
SmiMI	CAYNNNNRTG	3 cut(s)	615, 767, 773
Sse8387I	CCTGCAGG	1 cut(s)	657
Sse9I	AATT	8 cut(s)	152, 309, 421, 534, 794, 940, 990, 1235
SsiI	CCGC	2 cut(s)	659, 840
SspMI	CTAG	1 cut(s)	146
StyD4I	CCNGG	1 cut(s)	967
StyI	CCWWGG	2 cut(s)	279, 1131
TaaI	ACNGT	4 cut(s)	64, 434, 921, 1099
TaqI	TCGA	8 cut(s)	252, 354, 412, 544, 598, 665, 821, 887
TasI	AATT	8 cut(s)	152, 309, 421, 534, 794, 940, 990, 1235
TatI	WGTACW	2 cut(s)	298, 932
TfiI	GAWTC	4 cut(s)	182, 406, 539, 1136
Tru1I	TTAA	4 cut(s)	75, 168, 960, 1227
Tru9I	TTAA	4 cut(s)	75, 168, 960, 1227
TscAI	CASTG	2 cut(s)	67, 439
TseFI	GTSAC	5 cut(s)	58, 218, 232, 703, 963
TseI	GCWGC	2 cut(s)	742, 826
Tsp45I	GTSAC	5 cut(s)	58, 218, 232, 703, 963
TspDTI	ATGAA	3 cut(s)	279, 345, 456
TspGWI	ACGGA	1 cut(s)	390
TspRI	CASTG	2 cut(s)	67, 439
Tth111I	GACNNNGTC	1 cut(s)	856
XapI	RAATTY	2 cut(s)	152, 309
XcmI	CCANNNNNNNNNTGG	1 cut(s)	301
XmiI	GTMKAC	1 cut(s)	873
XmnI	GAANNNNTTC	1 cut(s)	108
XspI	CTAG	1 cut(s)	146
ZrmI	AGTACT	1 cut(s)	934

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.