Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007080

Physical Location & Seq

Genomic Coordinates Reverse (-)

32500080 .. 32502835

Gene Structure 2756 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1258000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGAAGATCCAAGTGGGGAGTCCGAGCTGGAAGAAGACTTTCCAGAAGCAAACTGTGGTAAGATGAGGTGGATCTTGAACAAAGGATGGGGACTTGGGAAGAAAGTTCTGGTGACTGGTTTGGTAATTTCTTCTGCCCCAATTGTTCTTCCGCCGCTTGTGGTTATTTCCGCAATTGGGCTTGCCCTTTCTGTTCCATCCGGCATTATCTTGGCAAGTTATGCTTGCAGTGAGAAGATCATGAGCAAGTTACTTCCATATAGTGAGCCGCCTCCTTTGTTGGAAAATAATGTAACGTTAATGTCGAATAACGAAGAATTGCAGCATGACGAGGAATGGGAACGGGGTATGTGGGTAGGAGCGAAGACTGATATCGAAAAGGAAGAAGGATGGGATTGGACGGATCGACTCGAAGGCCGTGAGATGAGAGTTGAATTTGTTGATGAGGAAACTGGTGACATTATTGAGGAGGCTGCGCAAGGAAATGTAACTGAAGATGTGGGTGAAATTGAATTTGTAGAAGAAAATGGATATGAGAGGGACGTAGATGAGTCTACGGATGAGGAGGAAAAGCTAATATTGGATAGTATTTATGAGGTGAAAATTAAAGGATTGAGTGAGGATTTTAAGGATGATTCCGTACTAGATGAGAATCAAGTCGATGAGGTGTGTGGATTAGTGTTCAATGTAATTGAGGGTGATGTGCAAATGGGAGTGATACCCACTGAAGGGACTACCGTAGCAATCAAAGAAAGCGAGGGTCAAGAAAGAGTAAATGATATAGTAGAAGAGGCGGAGTTGGTGGAAGAGACGAGGGGCTTGCTAGAAAAAATTAGGGATGAAGGCAACACTGATAATGCAATGGAGATGGACAAGCGGTATGTGGGAGAAAGTGAAGGAGGTGGGGAGCTAATCAATCAAAAAGTTGGTTCAAATGCTGAAGAGATTGAGATACCAGTTGAAGCTAGAATTGCTGACATTGGATCGCAAGAATCAACTAATACCAGGGAAGGTGGAGTTGGGGACGTTCCAGAGACCGTTTATGAGGATGTCAAACCGGTTAGAGATATTGGGAGTGTTTCGGAAAGCAAGAATGCAGAGAATGATTTAGCAGCCGAGAAGCCAATAGGGGAAACCAAGAATAATGTTAAAGACGTTTTAGTGGAAGAAAAATCAAGGGCAAAAACAAGGAATTTGAAGCCTTTGGCGAGCAGCTTCACGGAAGACAAGCAAAAGTTTGTGATAAACAAAGAGGAAATGGTACAAGGAAGGAGAACCGATGATACAATCAATGAATTAGAGTTTCAGTTGATTAGAGAGAAGGAAATTGTGGTTTCAAATGCAGATGCAAGAGAGATTGCTGATGAAAGCGGGCTTCATTTGTTTGATGAGAAGAATCAATCTGGTCAGCAAGACTGGCATTCTTCTTATGGAATTTCTCAAGGTCATGTGGGTGATGAAGGTGCAGATTATGCAGAGCTTCCCATTTCAAGCATTGCACGTGAATCTACAGATTCTGGAATATCATCCGGAAAGGACATTACAATGCCTTCAAATGGGGTACTGTATGATGAGACGAAAATATGGGCGAAGATCGACGCTATACGAAGTATAGTCGGATACAAAGGCACACCCCACGCAACATGCATAGAGGAACTGAAGGCTCTTTACCTCTTCACAGGTGTGGAACCCCCTACAGTATCATTTAGTGACCCTCCTGATCTCGCACAGGTTAATCACAATCTTCGCTTTCTCATGTCCATAATCGGAGTTAAATTGGACTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

596

Amino Acids

65.75

Weight (kDa)

4.29

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65090 AT1G65090 AT1G65090 AT5G36100 AT5G36100
fragaria_vesca	FvH4_6g00830 FvH4_6g00830
malus_domestica	MD04G1241000.v1.1 MD12G1258000.v1.1
prunus_persica	Prupe.6G358400_v2.0.a1 Prupe.6G358500_v2.0.a1 Prupe.6G358500_v2.0.a1
pyrus_communis	pycom04g21270 pycom12g23660
rosa_chinensis	RchiOBHm_Chr3g0448301
rosa_laevigata	RLG00000025929
rosa_multiflora	Rmu_co8397881.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426450
rosa_rugosa	Rorug02G0610100
rosa_samantha	Rh3AG009800 Rh3BG009300 Rh3BG009400 Rh3CG009300 Rh3DG010000
rosa_wichuraiana	Rw3G000790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	477
AccI	GTMKAC	1 cut(s)	554
AccIII	TCCGGA	1 cut(s)	1529
AciI	CCGC	7 cut(s)	152, 155, 171, 269, 794, 877, 1371
AclI	AACGTT	1 cut(s)	296
AclWI	GGATC	4 cut(s)	2, 80, 411, 991
AcsI	RAATTY	4 cut(s)	434, 512, 1192, 1434
AcuI	CTGAAG	4 cut(s)	513, 747, 960, 1679
AcvI	CACGTG	1 cut(s)	1502
AfaI	GTAC	3 cut(s)	642, 1263, 1563
AfiI	CCNNNNNNNGG	4 cut(s)	177, 728, 729, 1556
AgeI	ACCGGT	1 cut(s)	1057
AjnI	CCWGG	1 cut(s)	1004
AjuI	GAANNNNNNNTTGG	2 cut(s)	1002, 1034
AleI	CACNNNNGTG	1 cut(s)	1682
AluBI	AGCT	6 cut(s)	28, 574, 910, 965, 1215, 1480
AluI	AGCT	6 cut(s)	28, 574, 910, 965, 1215, 1480
Alw26I	GTCTC	3 cut(s)	803, 1028, 1568
AlwI	GGATC	4 cut(s)	2, 80, 411, 991
AlwNI	CAGNNNCTG	1 cut(s)	1517
Aor13HI	TCCGGA	1 cut(s)	1529
AoxI	GGCC	1 cut(s)	415
ApeKI	GCWGC	4 cut(s)	322, 473, 1112, 1212
ApoI	RAATTY	4 cut(s)	434, 512, 1192, 1434
AsiGI	ACCGGT	1 cut(s)	1057
Asp700I	GAANNNNTTC	1 cut(s)	39
AspLEI	GCGC	1 cut(s)	478
AsuHPI	GGTGA	6 cut(s)	124, 467, 515, 610, 710, 1466
BarI	GAAGNNNNNNTAC	2 cut(s)	1652, 1684
BauI	CACGAG	1 cut(s)	1783
BbrPI	CACGTG	1 cut(s)	1502
BbsI	GAAGAC	3 cut(s)	42, 371, 1230
BbvI	GCAGC	4 cut(s)	334, 460, 1124, 1224
BccI	CCATC	4 cut(s)	81, 205, 384, 862
BceAI	ACGGC	1 cut(s)	402
BcgI	CGANNNNNNTGC	2 cut(s)	802, 836
BciT130I	CCWGG	1 cut(s)	1006
BciVI	GTATCC	1 cut(s)	1613
BcoDI	GTCTC	3 cut(s)	803, 1028, 1568
BfaI	CTAG	3 cut(s)	644, 824, 966
BfmI	CTRYAG	2 cut(s)	1509, 1695
BfuI	GTATCC	1 cut(s)	1613
BisI	GCNGC	6 cut(s)	155, 269, 323, 474, 1113, 1213
BlsI	GCNGC	6 cut(s)	156, 270, 324, 475, 1114, 1214
Bme1390I	CCNGG	1 cut(s)	1006
BmiI	GGNNCC	1 cut(s)	1689
BmrFI	CCNGG	1 cut(s)	1006
BmsI	GCATC	1 cut(s)	1336
BpiI	GAAGAC	3 cut(s)	42, 371, 1230
BpuEI	CTTGAG	1 cut(s)	1425
BsaAI	YACGTR	1 cut(s)	1502
BsaBI	GATNNNNATC	1 cut(s)	651
BsaI	GGTCTC	1 cut(s)	1028
BsaJI	CCNNGG	1 cut(s)	1005
BsaWI	WCCGGW	2 cut(s)	1057, 1529
Bsc4I	CCNNNNNNNGG	4 cut(s)	177, 728, 729, 1556
Bse118I	RCCGGY	1 cut(s)	1057
Bse1I	ACTGG	4 cut(s)	121, 457, 956, 1421
Bse3DI	GCAATG	2 cut(s)	867, 1494
Bse8I	GATNNNNATC	1 cut(s)	651
BseAI	TCCGGA	1 cut(s)	1529
BseBI	CCWGG	1 cut(s)	1006
BseDI	CCNNGG	1 cut(s)	1005
BseGI	GGATG	8 cut(s)	92, 197, 395, 565, 637, 844, 1054, 1526
BseJI	GATNNNNATC	1 cut(s)	651
BseLI	CCNNNNNNNGG	4 cut(s)	177, 728, 729, 1556
BseMI	GCAATG	2 cut(s)	867, 1494
BseNI	ACTGG	4 cut(s)	121, 457, 956, 1421
BseRI	GAGGAG	2 cut(s)	482, 578
BseXI	GCAGC	4 cut(s)	334, 460, 1124, 1224
BsgI	GTGCAG	1 cut(s)	1485
BshFI	GGCC	1 cut(s)	417
BshTI	ACCGGT	1 cut(s)	1057
BsiSI	CCGG	3 cut(s)	201, 1058, 1530
BslFI	GGGAC	4 cut(s)	105, 554, 745, 1037
BslI	CCNNNNNNNGG	4 cut(s)	177, 728, 729, 1556
BsmAI	GTCTC	3 cut(s)	803, 1028, 1568
BsmBI	CGTCTC	2 cut(s)	803, 1568
BsmFI	GGGAC	4 cut(s)	105, 554, 745, 1037
BsmI	GAATGC	2 cut(s)	1099, 1420
BsnI	GGCC	1 cut(s)	417
Bso31I	GGTCTC	1 cut(s)	1028
Bsp13I	TCCGGA	1 cut(s)	1529
Bsp143I	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
BspACI	CCGC	7 cut(s)	152, 155, 171, 269, 794, 877, 1371
BspANI	GGCC	1 cut(s)	417
BspEI	TCCGGA	1 cut(s)	1529
BspHI	TCATGA	1 cut(s)	240
BspLI	GGNNCC	1 cut(s)	1689
BspPI	GGATC	4 cut(s)	2, 80, 411, 991
BspTNI	GGTCTC	1 cut(s)	1028
BsrDI	GCAATG	2 cut(s)	867, 1494
BsrFI	RCCGGY	1 cut(s)	1057
BsrI	ACTGG	4 cut(s)	121, 457, 956, 1421
BssAI	RCCGGY	1 cut(s)	1057
BssECI	CCNNGG	1 cut(s)	1005
BssMI	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
BssSI	CACGAG	1 cut(s)	1783
Bst2BI	CACGAG	1 cut(s)	1783
Bst2UI	CCWGG	1 cut(s)	1006
Bst4CI	ACNGT	5 cut(s)	56, 739, 1039, 1566, 1699
Bst6I	CTCTTC	4 cut(s)	783, 801, 936, 1679
BstAPI	GCANNNNNTGC	2 cut(s)	221, 1472
BstBAI	YACGTR	1 cut(s)	1502
BstC8I	GCNNGC	5 cut(s)	183, 226, 821, 1210, 1373
BstF5I	GGATG	8 cut(s)	92, 197, 395, 565, 637, 844, 1054, 1526
BstHHI	GCGC	1 cut(s)	478
BstKTI	GATC	7 cut(s)	10, 75, 240, 406, 986, 1596, 1723
BstMAI	GTCTC	3 cut(s)	803, 1028, 1568
BstMBI	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
BstMWI	GCNNNNNNNGC	4 cut(s)	221, 971, 1417, 1472
BstNI	CCWGG	1 cut(s)	1006
BstNSI	RCATGY	1 cut(s)	1647
BstSCI	CCNGG	1 cut(s)	1004
BstSFI	CTRYAG	2 cut(s)	1509, 1695
BstV1I	GCAGC	4 cut(s)	334, 460, 1124, 1224
BstV2I	GAAGAC	3 cut(s)	42, 371, 1230
BstX2I	RGATCY	2 cut(s)	7, 72
BstYI	RGATCY	2 cut(s)	7, 72
BsuI	GTATCC	1 cut(s)	1613
BsuRI	GGCC	1 cut(s)	417
BtsCI	GGATG	8 cut(s)	92, 197, 395, 565, 637, 844, 1054, 1526
BtsI	GCAGTG	1 cut(s)	235
BtsIMutI	CAGTG	3 cut(s)	235, 723, 849
Cac8I	GCNNGC	5 cut(s)	183, 226, 821, 1210, 1373
CaiI	CAGNNNCTG	1 cut(s)	1517
CciI	TCATGA	1 cut(s)	240
CfoI	GCGC	1 cut(s)	478
Cfr10I	RCCGGY	1 cut(s)	1057
CseI	GACGC	1 cut(s)	1607
Csp6I	GTAC	3 cut(s)	641, 1262, 1562
CspAI	ACCGGT	1 cut(s)	1057
CviAII	CATG	5 cut(s)	241, 326, 1448, 1644, 1756
CviQI	GTAC	3 cut(s)	641, 1262, 1562
DpnI	GATC	7 cut(s)	9, 74, 239, 405, 985, 1595, 1722
DpnII	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
Eam1104I	CTCTTC	4 cut(s)	783, 801, 936, 1679
EarI	CTCTTC	4 cut(s)	783, 801, 936, 1679
EciI	GGCGGA	2 cut(s)	141, 809
Eco31I	GGTCTC	1 cut(s)	1028
Eco32I	GATATC	1 cut(s)	373
Eco57I	CTGAAG	4 cut(s)	513, 747, 960, 1679
Eco72I	CACGTG	1 cut(s)	1502
EcoRII	CCWGG	1 cut(s)	1004
EcoRV	GATATC	1 cut(s)	373
EcoT22I	ATGCAT	1 cut(s)	1649
Esp3I	CGTCTC	2 cut(s)	803, 1568
FaeI	CATG	5 cut(s)	244, 329, 1451, 1647, 1759
FalI	AAGNNNNNCTT	2 cut(s)	208, 240
FaqI	GGGAC	4 cut(s)	105, 554, 745, 1037
FatI	CATG	5 cut(s)	240, 325, 1447, 1643, 1755
FauI	CCCGC	1 cut(s)	1364
FblI	GTMKAC	1 cut(s)	554
Fnu4HI	GCNGC	6 cut(s)	155, 269, 323, 474, 1113, 1213
FokI	GGATG	8 cut(s)	99, 184, 402, 572, 644, 851, 1061, 1513
Fsp4HI	GCNGC	6 cut(s)	155, 269, 323, 474, 1113, 1213
FspBI	CTAG	3 cut(s)	644, 824, 966
FspI	TGCGCA	1 cut(s)	477
GlaI	GCGC	1 cut(s)	477
GluI	GCNGC	6 cut(s)	155, 269, 323, 474, 1113, 1213
HaeIII	GGCC	1 cut(s)	417
HapII	CCGG	3 cut(s)	201, 1058, 1530
HgaI	GACGC	1 cut(s)	1607
HhaI	GCGC	1 cut(s)	478
Hin1II	CATG	5 cut(s)	244, 329, 1451, 1647, 1759
Hin6I	GCGC	1 cut(s)	476
HinP1I	GCGC	1 cut(s)	476
HpaII	CCGG	3 cut(s)	201, 1058, 1530
HphI	GGTGA	6 cut(s)	124, 467, 515, 610, 710, 1466
Hpy166II	GTNNAC	1 cut(s)	555
Hpy188I	TCNGA	4 cut(s)	25, 1084, 1619, 1769
Hpy188III	TCNNGA	9 cut(s)	44, 76, 241, 764, 1031, 1518, 1530, 1718, 1785
Hpy8I	GTNNAC	1 cut(s)	555
Hpy99I	CGWCG	1 cut(s)	1601
HpyCH4III	ACNGT	5 cut(s)	56, 739, 1039, 1566, 1699
HpyCH4IV	ACGT	5 cut(s)	296, 543, 1026, 1155, 1501
HpyF10VI	GCNNNNNNNGC	4 cut(s)	221, 971, 1417, 1472
HpySE526I	ACGT	5 cut(s)	296, 543, 1026, 1155, 1501
Hsp92II	CATG	5 cut(s)	244, 329, 1451, 1647, 1759
HspAI	GCGC	1 cut(s)	476
Kpn2I	TCCGGA	1 cut(s)	1529
Kzo9I	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
LmnI	GCTCC	2 cut(s)	359, 907
Lsp1109I	GCAGC	4 cut(s)	334, 460, 1124, 1224
LweI	GCATC	1 cut(s)	1336
MaeI	CTAG	3 cut(s)	644, 824, 966
MaeII	ACGT	5 cut(s)	296, 543, 1026, 1155, 1501
MaeIII	GTNAC	6 cut(s)	112, 249, 292, 455, 487, 1709
MalI	GATC	7 cut(s)	9, 74, 239, 405, 985, 1595, 1722
MboI	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
MfeI	CAATTG	2 cut(s)	141, 174
MflI	RGATCY	2 cut(s)	7, 72
MlyI	GAGTC	4 cut(s)	29, 402, 560, 1775
MmeI	TCCRAC	2 cut(s)	261, 1597
Mph1103I	ATGCAT	1 cut(s)	1649
MroI	TCCGGA	1 cut(s)	1529
MroXI	GAANNNNTTC	1 cut(s)	39
MseI	TTAA	6 cut(s)	299, 606, 627, 1149, 1734, 1773
MslI	CAYNNNNRTG	3 cut(s)	1452, 1544, 1682
MspI	CCGG	3 cut(s)	201, 1058, 1530
MspR9I	CCNGG	1 cut(s)	1006
MunI	CAATTG	2 cut(s)	141, 174
Mva1269I	GAATGC	2 cut(s)	1099, 1420
MvaI	CCWGG	1 cut(s)	1006
MwoI	GCNNNNNNNGC	4 cut(s)	221, 971, 1417, 1472
NdeII	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
NlaIII	CATG	5 cut(s)	244, 329, 1451, 1647, 1759
NlaIV	GGNNCC	1 cut(s)	1689
NmeAIII	GCCGAG	1 cut(s)	1141
NmuCI	GTSAC	3 cut(s)	112, 455, 1709
NsbI	TGCGCA	1 cut(s)	477
NsiI	ATGCAT	1 cut(s)	1649
NspI	RCATGY	1 cut(s)	1647
OliI	CACNNNNGTG	1 cut(s)	1682
PagI	TCATGA	1 cut(s)	240
PcsI	WCGNNNNNNNCGW	1 cut(s)	302
PctI	GAATGC	2 cut(s)	1099, 1420
PdmI	GAANNNNTTC	1 cut(s)	39
PfeI	GAWTC	6 cut(s)	635, 652, 992, 1396, 1505, 1514
PinAI	ACCGGT	1 cut(s)	1057
PkrI	GCNGC	6 cut(s)	156, 270, 324, 475, 1114, 1214
PleI	GAGTC	4 cut(s)	28, 402, 559, 1775
PmaCI	CACGTG	1 cut(s)	1502
PmlI	CACGTG	1 cut(s)	1502
PpsI	GAGTC	4 cut(s)	28, 402, 559, 1775
Ppu21I	YACGTR	1 cut(s)	1502
Psp1406I	AACGTT	1 cut(s)	296
Psp6I	CCWGG	1 cut(s)	1004
PspCI	CACGTG	1 cut(s)	1502
PspGI	CCWGG	1 cut(s)	1004
PspN4I	GGNNCC	1 cut(s)	1689
PsrI	GAACNNNNNNTAC	2 cut(s)	1267, 1299
PstNI	CAGNNNCTG	1 cut(s)	1517
PsuI	RGATCY	2 cut(s)	7, 72
RsaI	GTAC	3 cut(s)	642, 1263, 1563
RsaNI	GTAC	3 cut(s)	641, 1262, 1562
RseI	CAYNNNNRTG	3 cut(s)	1452, 1544, 1682
SaqAI	TTAA	6 cut(s)	299, 606, 627, 1149, 1734, 1773
SatI	GCNGC	6 cut(s)	155, 269, 323, 474, 1113, 1213
Sau3AI	GATC	7 cut(s)	7, 72, 237, 403, 983, 1593, 1720
SchI	GAGTC	4 cut(s)	29, 402, 560, 1775
ScrFI	CCNGG	1 cut(s)	1006
SfaNI	GCATC	1 cut(s)	1336
SfcI	CTRYAG	2 cut(s)	1509, 1695
SmiMI	CAYNNNNRTG	3 cut(s)	1452, 1544, 1682
SmlI	CTYRAG	1 cut(s)	1440
SmoI	CTYRAG	1 cut(s)	1440
SsiI	CCGC	7 cut(s)	152, 155, 171, 269, 794, 877, 1371
SspI	AATATT	1 cut(s)	579
SspMI	CTAG	3 cut(s)	644, 824, 966
StyD4I	CCNGG	1 cut(s)	1004
TaaI	ACNGT	5 cut(s)	56, 739, 1039, 1566, 1699
TaiI	ACGT	5 cut(s)	299, 546, 1029, 1158, 1504
TaqI	TCGA	6 cut(s)	305, 375, 406, 411, 660, 1596
TauI	GCSGC	2 cut(s)	157, 271
TfiI	GAWTC	6 cut(s)	635, 652, 992, 1396, 1505, 1514
Tru1I	TTAA	6 cut(s)	299, 606, 627, 1149, 1734, 1773
Tru9I	TTAA	6 cut(s)	299, 606, 627, 1149, 1734, 1773
TscAI	CASTG	3 cut(s)	235, 730, 856
TseFI	GTSAC	3 cut(s)	112, 455, 1709
TseI	GCWGC	4 cut(s)	322, 473, 1112, 1212
Tsp45I	GTSAC	3 cut(s)	112, 455, 1709
TspDTI	ATGAA	5 cut(s)	855, 1308, 1367, 1380, 1473
TspGWI	ACGGA	4 cut(s)	416, 572, 628, 1235
TspRI	CASTG	3 cut(s)	235, 730, 856
XapI	RAATTY	4 cut(s)	434, 512, 1192, 1434
XceI	RCATGY	1 cut(s)	1647
XmiI	GTMKAC	1 cut(s)	554
XmnI	GAANNNNTTC	1 cut(s)	39
XspI	CTAG	3 cut(s)	644, 824, 966
Zsp2I	ATGCAT	1 cut(s)	1649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.