MD13G1009800.v1.1

Remorin, C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004396
Physical Location & Seq
Forward (+)
623332 .. 627178
3847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1009800.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGATTACGAAAGGATCCAAAAGCCTCAGGTGGGCGGTGGGGGATTTTCCCCAGGGAAGCTGAGGAGTATGCTACTGGGAGTAGAGAAGAAGAGGAAGGAGAATGAAGATACTGAATGTGCATTCACTCTGAGATCTCAGCCTCCTGACTTTGATGATCAAGCTGGGGGTGGTGCATCTGATAACTGCAAAGATGTGGATGTTGTGAGTGTTCATCCCAAATGCTCGACTTTGAATGCTGCTGACTCATTAGGCCCGGAGATGGGTAGCGACCAGCGATTGAAGGATAGCAGATTAGTCAATTCAAGAATTAGGAGCCAAGAAGACCCTTCTTTAGATTATGGTAGTGGGTGCGATGCTATGAGTGTGTCCGCCTCCATGTTTGAGTTTCAGAAGGCAGAGCGTGCCCCACAAAGGGTGCCTCTTGCCCCATTCTCCAAACCAGCGCCGTCCAAATGGGATGATGCGCAGAAGTGGATAGCGAGCCCAACTTGGAACCGGCCAAAGATGGGACAGGTTCAAATGCAGGGTGGGCAAGGGGTTGGGTTGCAAAAGGTGGGTAGCTTTGGTTATGGGAGTGGACAATCGAGCTGTAGCACAAAGGTGGTTGTTGAAGTTCCAGATCAAAAAGTAGATATTTTTGAAGAACCTGATACGAAAAGAATTGACACAAGCCAACCTAAGACGGCAAATGGGGGGCAGAAGTGTGTAAGTTGGGAGCCTGATCCATACCCTATTGCCGATTCATATGGCAAATCGGTGCTCATGGTTGAGAGCTCTGTCGGAGAGTCGGCAATCAATCTTAGCCAGCATGATTCTTCTACAGCCTTTCATGGAACAACAATATTTATTCCTCCTCCTTCAACAGCAAGGTCGGTGTCAATGAGAGACATGGGGACAGAGATGACACCTATAGCTAGTCAGGAACCCTCCAGGACTGGAACTCCCGTGGGGGCAACAACACCAATTCGCAGTCCTAATAATTCAAGTCCATCAACTCCTAGCAGAGCTGCACCCACTTCATCTCCGATTGACCAAAATACTAACAAGGAGTTGTCTGAAAAGGAGTTACAATTGAAAACTAGGAGAGAGATAATGGTTCTTGGGACGCAGCTGGGTAAGATGAACATCGCTGCTTGGGCTAGCAAAGAGGAGGATAAGGATGCATCCGCGTCACAGAAAACTGTACTGGAAGAACAGCCGGGTACAAGTGTTATTGAGACACGTGCAGCAGCATGGGAGGAATCTGAGAAAGCCAAGTATATGGCTAGGTTCAAGCGTGAAGAGATGAAAATTCAAGCTTGGGAAAACCATCAGAAAGCGAAAACAGAAGCTGAGATGAGGAAAATCGAGGTCGAGGTTGAAAGGATAAGAGGACGCGCTCATGGTAAGCTTATGAATAAGTTAGCAGCTGCAAGACATAAAGCTCAAGAAAAGCGAACTGCGGCAGAGGCCAAGAGAAACCGGCAAGCAGCAAAAGCAGAGCATCAAGCAGAGTACATTCGCAAAACCGGTCGCATCCCGTCCTCGTTTTGGTGTTGGGGTTGGTGCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Remorin_C

Protein Analysis

519

Amino Acids

56.92

Weight (kDa)

8.55

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Remorin_C PF03763 406 - 509 1.9e-34 Remorin, C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 418
AccII CGCG 2 cut(s) 1172, 1380
AciI CCGC 4 cut(s) 36, 372, 1170, 1445
AclWI GGATC 3 cut(s) 10, 23, 719
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 1 cut(s) 1293
AcvI CACGTG 1 cut(s) 1226
AfaI GTAC 3 cut(s) 1188, 1207, 1499
AfiI CCNNNNNNNGG 7 cut(s) 32, 262, 263, 414, 415, 509, 1533
AflIII ACRYGT 1 cut(s) 1223
AgeI ACCGGT 1 cut(s) 1511
AjnI CCWGG 2 cut(s) 52, 932
AleI CACNNNNGTG 1 cut(s) 602
Alw21I GWGCWC 2 cut(s) 765, 779
Alw26I GTCTC 2 cut(s) 882, 1214
AlwI GGATC 3 cut(s) 10, 23, 719
AlwNI CAGNNNCTG 1 cut(s) 1334
AoxI GGCC 3 cut(s) 253, 500, 1452
ApeKI GCWGC 9 cut(s) 239, 1010, 1111, 1133, 1229, 1232, 1409, 1412, 1472
ApoI RAATTY 1 cut(s) 1293
AsiGI ACCGGT 1 cut(s) 1511
AspLEI GCGC 3 cut(s) 448, 469, 1382
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 2 cut(s) 257, 1203
AsuNHI GCTAGC 1 cut(s) 1142
AxyI CCTNAGG 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 409
BaeI ACNNNNGTAYC 2 cut(s) 1197, 1230
BamHI GGATCC 1 cut(s) 15
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 2 cut(s) 488, 779
BbrPI CACGTG 1 cut(s) 1226
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 2 cut(s) 765, 779
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 9 cut(s) 226, 997, 1120, 1123, 1241, 1244, 1399, 1421, 1484
BccI CCATC 4 cut(s) 256, 502, 1000, 1320
BceAI ACGGC 2 cut(s) 433, 702
BciT130I CCWGG 2 cut(s) 54, 934
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 2 cut(s) 257, 1203
BcoDI GTCTC 2 cut(s) 882, 1214
BfaI CTAG 5 cut(s) 918, 1002, 1083, 1143, 1269
BfmI CTRYAG 3 cut(s) 592, 822, 912
BfoI RGCGCY 1 cut(s) 449
BglII AGATCT 1 cut(s) 134
Bme1390I CCNGG 4 cut(s) 54, 257, 934, 1203
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 6 cut(s) 17, 317, 420, 497, 720, 927
BmrFI CCNGG 4 cut(s) 54, 257, 934, 1203
BmrI ACTGGG 1 cut(s) 86
BmsI GCATC 7 cut(s) 185, 346, 454, 1153, 1175, 1495, 1527
BmtI GCTAGC 1 cut(s) 1146
BmuI ACTGGG 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 916
Bpu10I CCTNAGC 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 1413
BpuMI CCSGG 2 cut(s) 257, 1203
BsaAI YACGTR 1 cut(s) 1226
BsaBI GATNNNNATC 1 cut(s) 1127
BsaJI CCNNGG 3 cut(s) 52, 53, 948
BsaWI WCCGGW 1 cut(s) 1511
BsaXI ACNNNNNCTCC 4 cut(s) 928, 958, 1009, 1039
Bsc4I CCNNNNNNNGG 7 cut(s) 32, 262, 263, 414, 415, 509, 1533
Bse118I RCCGGY 3 cut(s) 498, 1464, 1511
Bse1I ACTGG 3 cut(s) 81, 943, 1194
Bse21I CCTNAGG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 1127
BseBI CCWGG 2 cut(s) 54, 934
BseDI CCNNGG 3 cut(s) 52, 53, 948
BseGI GGATG 6 cut(s) 205, 214, 466, 1166, 1168, 1518
BseJI GATNNNNATC 1 cut(s) 1127
BseLI CCNNNNNNNGG 7 cut(s) 32, 262, 263, 414, 415, 509, 1533
BseMII CTCAG 6 cut(s) 41, 53, 122, 152, 1239, 1326
BseNI ACTGG 3 cut(s) 81, 943, 1194
BseRI GAGGAG 3 cut(s) 79, 846, 1166
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 9 cut(s) 226, 997, 1120, 1123, 1241, 1244, 1399, 1421, 1484
BseYI CCCAGC 2 cut(s) 164, 1114
BsgI GTGCAG 2 cut(s) 996, 1248
Bsh1236I CGCG 2 cut(s) 1172, 1380
Bsh1285I CGRYCG 1 cut(s) 1516
BshFI GGCC 3 cut(s) 255, 502, 1454
BshNI GGYRCC 1 cut(s) 418
BshTI ACCGGT 1 cut(s) 1511
BsiEI CGRYCG 1 cut(s) 1516
BsiHKAI GWGCWC 2 cut(s) 765, 779
BsiSI CCGG 5 cut(s) 257, 499, 1202, 1465, 1512
BslFI GGGAC 3 cut(s) 525, 910, 1120
BslI CCNNNNNNNGG 7 cut(s) 32, 262, 263, 414, 415, 509, 1533
BsmAI GTCTC 2 cut(s) 882, 1214
BsmFI GGGAC 3 cut(s) 525, 910, 1120
BsmI GAATGC 2 cut(s) 122, 241
BsnI GGCC 3 cut(s) 255, 502, 1454
Bsp1286I GDGCHC 4 cut(s) 409, 488, 765, 779
Bsp143I GATC 5 cut(s) 15, 134, 157, 622, 724
BspACI CCGC 4 cut(s) 36, 372, 1170, 1445
BspANI GGCC 3 cut(s) 255, 502, 1454
BspCNI CTCAG 6 cut(s) 40, 54, 123, 151, 1240, 1327
BspFNI CGCG 2 cut(s) 1172, 1380
BspLI GGNNCC 6 cut(s) 17, 317, 420, 497, 720, 927
BspOI GCTAGC 1 cut(s) 1146
BspPI GGATC 3 cut(s) 10, 23, 719
BspT107I GGYRCC 1 cut(s) 418
BsrFI RCCGGY 3 cut(s) 498, 1464, 1511
BsrI ACTGG 3 cut(s) 81, 943, 1194
BssAI RCCGGY 3 cut(s) 498, 1464, 1511
BssECI CCNNGG 3 cut(s) 52, 53, 948
BssMI GATC 5 cut(s) 15, 134, 157, 622, 724
Bst2UI CCWGG 2 cut(s) 54, 934
Bst4CI ACNGT 1 cut(s) 1186
Bst6I CTCTTC 2 cut(s) 86, 1278
BstAPI GCANNNNNTGC 1 cut(s) 404
BstBAI YACGTR 1 cut(s) 1226
BstC8I GCNNGC 5 cut(s) 405, 484, 809, 1144, 1470
BstDEI CTNAG 8 cut(s) 27, 62, 131, 138, 681, 803, 1248, 1335
BstDSI CCRYGG 1 cut(s) 948
BstF5I GGATG 6 cut(s) 205, 214, 466, 1166, 1168, 1518
BstFNI CGCG 2 cut(s) 1172, 1380
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 3 cut(s) 448, 469, 1382
BstKTI GATC 5 cut(s) 18, 137, 160, 625, 727
BstMAI GTCTC 2 cut(s) 882, 1214
BstMBI GATC 5 cut(s) 15, 134, 157, 622, 724
BstMCI CGRYCG 1 cut(s) 1516
BstMWI GCNNNNNNNGC 5 cut(s) 404, 532, 1139, 1451, 1478
BstNI CCWGG 2 cut(s) 54, 934
BstSCI CCNGG 4 cut(s) 52, 255, 932, 1201
BstSFI CTRYAG 3 cut(s) 592, 822, 912
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 2 cut(s) 1172, 1380
BstV1I GCAGC 9 cut(s) 226, 997, 1120, 1123, 1241, 1244, 1399, 1421, 1484
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 2 cut(s) 15, 134
BstXI CCANNNNNNTGG 1 cut(s) 1264
BstYI RGATCY 2 cut(s) 15, 134
Bsu36I CCTNAGG 1 cut(s) 27
BsuRI GGCC 3 cut(s) 255, 502, 1454
BtgI CCRYGG 1 cut(s) 948
BtgZI GCGATG 2 cut(s) 369, 1114
BtsCI GGATG 6 cut(s) 205, 214, 466, 1166, 1168, 1518
Cac8I GCNNGC 5 cut(s) 405, 484, 809, 1144, 1470
CaiI CAGNNNCTG 1 cut(s) 1334
CfoI GCGC 3 cut(s) 448, 469, 1382
Cfr10I RCCGGY 3 cut(s) 498, 1464, 1511
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 3 cut(s) 1117, 1161, 1386
Csp6I GTAC 3 cut(s) 1187, 1206, 1498
CspAI ACCGGT 1 cut(s) 1511
CviAII CATG 7 cut(s) 379, 766, 812, 833, 892, 1236, 1385
CviQI GTAC 3 cut(s) 1187, 1206, 1498
DdeI CTNAG 8 cut(s) 27, 62, 131, 138, 681, 803, 1248, 1335
DpnI GATC 5 cut(s) 17, 136, 159, 624, 726
DpnII GATC 5 cut(s) 15, 134, 157, 622, 724
EaeI YGGCCR 1 cut(s) 500
Eam1104I CTCTTC 2 cut(s) 86, 1278
EarI CTCTTC 2 cut(s) 86, 1278
EciI GGCGGA 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 777
Eco24I GRGCYC 2 cut(s) 488, 779
Eco53kI GAGCTC 1 cut(s) 777
Eco72I CACGTG 1 cut(s) 1226
Eco81I CCTNAGG 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 777
EcoRII CCWGG 2 cut(s) 52, 932
EcoT22I ATGCAT 1 cut(s) 1168
EcoT38I GRGCYC 2 cut(s) 488, 779
FaeI CATG 7 cut(s) 382, 769, 815, 836, 895, 1239, 1388
FaqI GGGAC 3 cut(s) 525, 910, 1120
FatI CATG 7 cut(s) 378, 765, 811, 832, 891, 1235, 1384
FauNDI CATATG 1 cut(s) 748
FbaI TGATCA 1 cut(s) 157
FokI GGATG 6 cut(s) 201, 212, 473, 1153, 1175, 1505
FriOI GRGCYC 2 cut(s) 488, 779
FspBI CTAG 5 cut(s) 918, 1002, 1083, 1143, 1269
FspI TGCGCA 1 cut(s) 468
GlaI GCGC 3 cut(s) 447, 468, 1381
GsaI CCCAGC 2 cut(s) 168, 1118
GsuI CTGGAG 1 cut(s) 916
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 3 cut(s) 255, 502, 1454
HapII CCGG 5 cut(s) 257, 499, 1202, 1465, 1512
HgaI GACGC 3 cut(s) 1117, 1161, 1386
HhaI GCGC 3 cut(s) 448, 469, 1382
Hin1II CATG 7 cut(s) 382, 769, 815, 836, 895, 1239, 1388
Hin6I GCGC 3 cut(s) 446, 467, 1380
HinP1I GCGC 3 cut(s) 446, 467, 1380
HindIII AAGCTT 2 cut(s) 1299, 1391
HinfI GANTC 5 cut(s) 245, 743, 788, 815, 1244
HpaII CCGG 5 cut(s) 257, 499, 1202, 1465, 1512
Hpy166II GTNNAC 1 cut(s) 581
Hpy188I TCNGA 8 cut(s) 132, 181, 393, 785, 1029, 1060, 1249, 1317
Hpy188III TCNNGA 5 cut(s) 146, 306, 620, 923, 1430
Hpy8I GTNNAC 1 cut(s) 581
HpyAV CCTTC 5 cut(s) 91, 277, 339, 388, 870
HpyCH4III ACNGT 1 cut(s) 1186
HpyCH4IV ACGT 1 cut(s) 1225
HpyCH4V TGCA 9 cut(s) 122, 176, 189, 526, 550, 1013, 1166, 1229, 1415
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 532, 1139, 1451, 1478
HpyF3I CTNAG 8 cut(s) 27, 62, 131, 138, 681, 803, 1248, 1335
HpySE526I ACGT 1 cut(s) 1225
Hsp92II CATG 7 cut(s) 382, 769, 815, 836, 895, 1239, 1388
HspAI GCGC 3 cut(s) 446, 467, 1380
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 5 cut(s) 15, 134, 157, 622, 724
LmnI GCTCC 2 cut(s) 315, 718
Lsp1109I GCAGC 9 cut(s) 226, 997, 1120, 1123, 1241, 1244, 1399, 1421, 1484
LweI GCATC 7 cut(s) 185, 346, 454, 1153, 1175, 1495, 1527
MaeI CTAG 5 cut(s) 918, 1002, 1083, 1143, 1269
MaeII ACGT 1 cut(s) 1225
MaeIII GTNAC 2 cut(s) 1068, 1173
MalI GATC 5 cut(s) 17, 136, 159, 624, 726
MboI GATC 5 cut(s) 15, 134, 157, 622, 724
MboII GAAGA 8 cut(s) 100, 103, 119, 335, 656, 810, 1205, 1295
MfeI CAATTG 1 cut(s) 1073
MflI RGATCY 2 cut(s) 15, 134
MhlI GDGCHC 4 cut(s) 409, 488, 765, 779
MluCI AATT 7 cut(s) 301, 309, 663, 966, 982, 1073, 1293
MlyI GAGTC 2 cut(s) 239, 797
MmeI TCCRAC 1 cut(s) 763
Mph1103I ATGCAT 1 cut(s) 1168
MslI CAYNNNNRTG 1 cut(s) 602
MspA1I CMGCKG 2 cut(s) 1114, 1412
MspI CCGG 5 cut(s) 257, 499, 1202, 1465, 1512
MspR9I CCNGG 4 cut(s) 54, 257, 934, 1203
MunI CAATTG 1 cut(s) 1073
Mva1269I GAATGC 2 cut(s) 122, 241
MvaI CCWGG 2 cut(s) 54, 934
MvnI CGCG 2 cut(s) 1172, 1380
MwoI GCNNNNNNNGC 5 cut(s) 404, 532, 1139, 1451, 1478
NciI CCSGG 2 cut(s) 257, 1203
NdeI CATATG 1 cut(s) 748
NdeII GATC 5 cut(s) 15, 134, 157, 622, 724
NheI GCTAGC 1 cut(s) 1142
NlaIII CATG 7 cut(s) 382, 769, 815, 836, 895, 1239, 1388
NlaIV GGNNCC 6 cut(s) 17, 317, 420, 497, 720, 927
NmuCI GTSAC 1 cut(s) 1173
NsbI TGCGCA 1 cut(s) 468
NsiI ATGCAT 1 cut(s) 1168
OliI CACNNNNGTG 1 cut(s) 602
PasI CCCWGGG 1 cut(s) 53
PctI GAATGC 2 cut(s) 122, 241
PfeI GAWTC 3 cut(s) 743, 815, 1244
PfoI TCCNGGA 1 cut(s) 932
PinAI ACCGGT 1 cut(s) 1511
PleI GAGTC 2 cut(s) 239, 796
PmaCI CACGTG 1 cut(s) 1226
PmlI CACGTG 1 cut(s) 1226
PpsI GAGTC 2 cut(s) 239, 796
Ppu21I YACGTR 1 cut(s) 1226
Psp124BI GAGCTC 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 52, 932
PspCI CACGTG 1 cut(s) 1226
PspFI CCCAGC 2 cut(s) 164, 1114
PspGI CCWGG 2 cut(s) 52, 932
PspN4I GGNNCC 6 cut(s) 17, 317, 420, 497, 720, 927
PspPI GGNCC 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 1334
PsuI RGATCY 2 cut(s) 15, 134
PvuII CAGCTG 2 cut(s) 1114, 1412
RsaI GTAC 3 cut(s) 1188, 1207, 1499
RsaNI GTAC 3 cut(s) 1187, 1206, 1498
RseI CAYNNNNRTG 1 cut(s) 602
SacI GAGCTC 1 cut(s) 779
Sau3AI GATC 5 cut(s) 15, 134, 157, 622, 724
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 2 cut(s) 239, 797
ScrFI CCNGG 4 cut(s) 54, 257, 934, 1203
SduI GDGCHC 4 cut(s) 409, 488, 765, 779
SfaNI GCATC 7 cut(s) 185, 346, 454, 1153, 1175, 1495, 1527
SfcI CTRYAG 3 cut(s) 592, 822, 912
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 1428
SmoI CTYRAG 1 cut(s) 1428
Sse9I AATT 7 cut(s) 301, 309, 663, 966, 982, 1073, 1293
SsiI CCGC 4 cut(s) 36, 372, 1170, 1445
SspI AATATT 1 cut(s) 846
SspMI CTAG 5 cut(s) 918, 1002, 1083, 1143, 1269
SstI GAGCTC 1 cut(s) 779
StyD4I CCNGG 4 cut(s) 52, 255, 932, 1201
TaaI ACNGT 1 cut(s) 1186
TaiI ACGT 1 cut(s) 1228
TaqI TCGA 4 cut(s) 227, 587, 1350, 1356
TasI AATT 7 cut(s) 301, 309, 663, 966, 982, 1073, 1293
TatI WGTACW 2 cut(s) 1186, 1497
TauI GCSGC 1 cut(s) 1448
TfiI GAWTC 3 cut(s) 743, 815, 1244
TseFI GTSAC 1 cut(s) 1173
TseI GCWGC 9 cut(s) 239, 1010, 1111, 1133, 1229, 1232, 1409, 1412, 1472
Tsp45I GTSAC 1 cut(s) 1173
TspDTI ATGAA 8 cut(s) 120, 203, 735, 821, 1011, 1139, 1304, 1412
XapI RAATTY 1 cut(s) 1293
XspI CTAG 5 cut(s) 918, 1002, 1083, 1143, 1269
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.