MD16G1008100.v1.1

Remorin, C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004396
Physical Location & Seq
Forward (+)
601705 .. 605496
3792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1008100.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGATATTATCACCCCCTTCCATGGATTACGAAAGGATCCAAAAACCTCAGGTGGGAGGTGGGGGATTTTCACCAGGGAAGCTGAGGAGTATGCTACTGGGAGTGGAGAAGAAGAGGAAGGAAAAGGAGGATTCTGAATCTGCATTCACTCTCAGATCTCAGCCTCCTGACTTTGATGATCAAGCTGGGGGTGGTGGTTCTGATGACTGTAAAGATGTAGATGTTGTGAGTGTTCTTCCCAAATGCTCGACTTCGAATGCTGCTGACTCATTAGGCCCGAAGATGGGTAGTGACCGGCGATTGAAGGATAGCGCATCAGTCAATTCAAGAATTAGGAACCAAGAAGACCCTTCATTAGATTATGATAGTGGGCATGATGCAATGGGTGTGTCCTCATCCATGTTTGAGTTTCAGAAGGCAGAGCGTGCCCCACAAAGGGTGCCTCTTGTGCCGTTCTCCAAACCAGCACCGTCCAAATGGGATGATGCTCAGAAATGGATAGCAAGCCCAACTTCGAACCGGCCAAAGATGGGGCAGGCTCAAATGCAGGGTGGGCAAGGAGTTGGATCGCGAAAGGCTAGTAGCTTTGGTTATGGGAGTAAACAATTGAGCACAAAGGTTGTTGTTGAAGTTCCAGATCAAAAAGTAGATATTTTTGAAGAACCAGATACGAAGCGAATTGACACAAATCAATCTAAGATGGAAAGTGGAGGGCAGAAGTATGTAAGTTGGGAGAATGATCCATACCCCATTGCCGATTCATATGGCAAATCAGTGCTCATGGTTGAGAACTCTGTCGGAGAGTCAGCAATCAATCTTAGCCAGCATGATTCTTCTACAGCGTTTCATGGAACAACCACATTTATTCCTCCTCCTTCAACCGCAAGATCTGTCTCAATGAGAGATATGGGTACAGAGATGACACCTATTGCTAGCCAGGAACCCTCCAGGACTGGAACTCCCGTGGGGGCAACAACACCGATTCGCAGTCCTACTAATTCAAGGCCATCAACTCCTAGCAGAGCTGCACAAGCTTCACCTCCGATCAACCAGAATCCTAACAAGGAGTTGTCTGAGAAGGAGTTACAAATGAAAACTAGGAGAGAGATAATGGTTCTTGGGACACAGTTGGGTAAGATGAACATCGCTGCTTGGGCTAGCAAAGAGGAGGATAAGGGTGCATCCACGTCACAGAAAACTGCACTGGAAGAACAACCAGGGAAAAGCATTGTTGAGACACGTGCAGCAGCATGGGAGGAAGCTGAGAAAGCCAAGTATATGGCTAGGTTCAAGCGTGAAGAGATGAAAATACAAGCTTGGGAAAATCATCAGAAGGCAAAAACAGAAGCTGAGATGAGGAAAATAGAGGTAGAGGTCGAAAGGATAAGAGGACGCGCTCATGGCAAGCTTATGAATAAGTTGGCAGCTGCAAGACATAAGGCTCAAGAAAAGCGAGCAGCAGCGGAGGCCAGGAGAAACCGGCAAGCAGCGAAAACAGAACATCAAGCAGAGTACATTCGCAAAACCGGTCGCATCCCGTCCTCGTTTTGGTGTTGGGGTTGGTGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Remorin_C

Protein Analysis

524

Amino Acids

57.62

Weight (kDa)

9.22

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Remorin_C PF03763 411 - 514 4.6e-35 Remorin, C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 439
AccII CGCG 2 cut(s) 571, 1395
AciI CCGC 2 cut(s) 882, 1463
AclWI GGATC 4 cut(s) 31, 44, 574, 734
AcoI YGGCCR 1 cut(s) 521
AcvI CACGTG 1 cut(s) 1241
AfaI GTAC 2 cut(s) 913, 1514
AfiI CCNNNNNNNGG 8 cut(s) 22, 53, 283, 284, 435, 436, 530, 1548
AflIII ACRYGT 1 cut(s) 1238
AgeI ACCGGT 1 cut(s) 1526
AgsI TTSAA 7 cut(s) 304, 327, 629, 659, 879, 1002, 1291
AjiI CACGTC 1 cut(s) 1188
AjnI CCWGG 5 cut(s) 73, 936, 947, 1216, 1469
Alw21I GWGCWC 3 cut(s) 614, 780, 1568
Alw26I GTCTC 2 cut(s) 898, 1229
AlwI GGATC 4 cut(s) 31, 44, 574, 734
AlwNI CAGNNNCTG 1 cut(s) 1349
AoxI GGCC 4 cut(s) 274, 521, 1004, 1467
AsiGI ACCGGT 1 cut(s) 1526
AspLEI GCGC 2 cut(s) 314, 1397
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 3 cut(s) 3, 63, 1029
AsuII TTCGAA 2 cut(s) 254, 515
AsuNHI GCTAGC 2 cut(s) 932, 1157
AxyI CCTNAGG 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 430
BamHI GGATCC 1 cut(s) 36
BanI GGYRCC 1 cut(s) 439
BbrPI CACGTG 1 cut(s) 1241
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 3 cut(s) 614, 780, 1568
BbvCI CCTCAGC 1 cut(s) 83
BccI CCATC 4 cut(s) 277, 523, 694, 1015
BceAI ACGGC 1 cut(s) 436
BciT130I CCWGG 5 cut(s) 75, 938, 949, 1218, 1471
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 898, 1229
BfaI CTAG 6 cut(s) 579, 933, 1017, 1098, 1158, 1284
BfmI CTRYAG 1 cut(s) 837
BglII AGATCT 2 cut(s) 155, 887
Bme1390I CCNGG 5 cut(s) 75, 938, 949, 1218, 1471
BmgBI CACGTC 1 cut(s) 1188
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 4 cut(s) 38, 338, 441, 942
BmrFI CCNGG 5 cut(s) 75, 938, 949, 1218, 1471
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 5 cut(s) 323, 367, 475, 1190, 1542
BmtI GCTAGC 2 cut(s) 936, 1161
BmuI ACTGGG 1 cut(s) 107
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 931
Bpu10I CCTNAGC 1 cut(s) 83
Bpu14I TTCGAA 2 cut(s) 254, 515
BpuEI CTTGAG 1 cut(s) 1428
BsaAI YACGTR 1 cut(s) 1241
BsaBI GATNNNNATC 1 cut(s) 1142
BsaJI CCNNGG 4 cut(s) 21, 74, 963, 1217
BsaWI WCCGGW 1 cut(s) 1526
BsaXI ACNNNNNCTCC 2 cut(s) 943, 973
Bsc4I CCNNNNNNNGG 8 cut(s) 22, 53, 283, 284, 435, 436, 530, 1548
Bse118I RCCGGY 4 cut(s) 294, 519, 1479, 1526
Bse1I ACTGG 3 cut(s) 102, 958, 1209
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 2 cut(s) 387, 750
Bse8I GATNNNNATC 1 cut(s) 1142
BseBI CCWGG 5 cut(s) 75, 938, 949, 1218, 1471
BseDI CCNNGG 4 cut(s) 21, 74, 963, 1217
BseGI GGATG 4 cut(s) 395, 487, 1181, 1533
BseJI GATNNNNATC 1 cut(s) 1142
BseLI CCNNNNNNNGG 8 cut(s) 22, 53, 283, 284, 435, 436, 530, 1548
BseMI GCAATG 2 cut(s) 387, 750
BseMII CTCAG 8 cut(s) 62, 74, 166, 173, 503, 1065, 1254, 1341
BseNI ACTGG 3 cut(s) 102, 958, 1209
BseRI GAGGAG 3 cut(s) 100, 861, 1181
BseSI GKGCMC 1 cut(s) 430
BseYI CCCAGC 1 cut(s) 185
BsgI GTGCAG 3 cut(s) 1011, 1185, 1263
Bsh1236I CGCG 2 cut(s) 571, 1395
Bsh1285I CGRYCG 1 cut(s) 1531
BshFI GGCC 4 cut(s) 276, 523, 1006, 1469
BshNI GGYRCC 1 cut(s) 439
BshTI ACCGGT 1 cut(s) 1526
BsiEI CGRYCG 1 cut(s) 1531
BsiHKAI GWGCWC 3 cut(s) 614, 780, 1568
BsiSI CCGG 4 cut(s) 295, 520, 1480, 1527
BslFI GGGAC 1 cut(s) 1135
BslI CCNNNNNNNGG 8 cut(s) 22, 53, 283, 284, 435, 436, 530, 1548
BsmAI GTCTC 2 cut(s) 898, 1229
BsmFI GGGAC 1 cut(s) 1135
BsmI GAATGC 2 cut(s) 143, 262
BsnI GGCC 4 cut(s) 276, 523, 1006, 1469
Bsp119I TTCGAA 2 cut(s) 254, 515
Bsp1286I GDGCHC 4 cut(s) 430, 614, 780, 1568
Bsp143I GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
Bsp19I CCATGG 1 cut(s) 21
Bsp68I TCGCGA 1 cut(s) 571
BspACI CCGC 2 cut(s) 882, 1463
BspANI GGCC 4 cut(s) 276, 523, 1006, 1469
BspCNI CTCAG 8 cut(s) 61, 75, 165, 172, 502, 1066, 1255, 1342
BspFNI CGCG 2 cut(s) 571, 1395
BspLI GGNNCC 4 cut(s) 38, 338, 441, 942
BspOI GCTAGC 2 cut(s) 936, 1161
BspPI GGATC 4 cut(s) 31, 44, 574, 734
BspT104I TTCGAA 2 cut(s) 254, 515
BspT107I GGYRCC 1 cut(s) 439
BsrDI GCAATG 2 cut(s) 387, 750
BsrFI RCCGGY 4 cut(s) 294, 519, 1479, 1526
BsrI ACTGG 3 cut(s) 102, 958, 1209
BssAI RCCGGY 4 cut(s) 294, 519, 1479, 1526
BssECI CCNNGG 4 cut(s) 21, 74, 963, 1217
BssMI GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 5 cut(s) 75, 938, 949, 1218, 1471
Bst4CI ACNGT 3 cut(s) 209, 471, 1128
Bst6I CTCTTC 2 cut(s) 107, 1293
BstAPI GCANNNNNTGC 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 1241
BstBI TTCGAA 2 cut(s) 254, 515
BstC8I GCNNGC 9 cut(s) 426, 505, 537, 824, 934, 1159, 1406, 1455, 1485
BstDSI CCRYGG 2 cut(s) 21, 963
BstF5I GGATG 4 cut(s) 395, 487, 1181, 1533
BstFNI CGCG 2 cut(s) 571, 1395
BstHHI GCGC 2 cut(s) 314, 1397
BstKTI GATC 8 cut(s) 39, 158, 181, 569, 640, 742, 890, 1047
BstMAI GTCTC 2 cut(s) 898, 1229
BstMBI GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
BstMCI CGRYCG 1 cut(s) 1531
BstMWI GCNNNNNNNGC 8 cut(s) 425, 448, 553, 1031, 1154, 1268, 1401, 1466
BstNI CCWGG 5 cut(s) 75, 938, 949, 1218, 1471
BstSCI CCNGG 5 cut(s) 73, 936, 947, 1216, 1469
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 430
BstUI CGCG 2 cut(s) 571, 1395
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 3 cut(s) 36, 155, 887
BstXI CCANNNNNNTGG 1 cut(s) 1279
BstYI RGATCY 3 cut(s) 36, 155, 887
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 4 cut(s) 276, 523, 1006, 1469
BtgI CCRYGG 2 cut(s) 21, 963
BtgZI GCGATG 1 cut(s) 1129
BtrI CACGTC 1 cut(s) 1188
BtsCI GGATG 4 cut(s) 395, 487, 1181, 1533
BtsIMutI CAGTG 2 cut(s) 780, 1202
BtuMI TCGCGA 1 cut(s) 571
Cac8I GCNNGC 9 cut(s) 426, 505, 537, 824, 934, 1159, 1406, 1455, 1485
CaiI CAGNNNCTG 1 cut(s) 1349
CfoI GCGC 2 cut(s) 314, 1397
Cfr10I RCCGGY 4 cut(s) 294, 519, 1479, 1526
Cfr13I GGNCC 1 cut(s) 275
CseI GACGC 1 cut(s) 1401
Csp6I GTAC 2 cut(s) 912, 1513
CspAI ACCGGT 1 cut(s) 1526
CviAII CATG 8 cut(s) 22, 374, 400, 781, 827, 848, 1251, 1400
CviQI GTAC 2 cut(s) 912, 1513
DpnI GATC 8 cut(s) 38, 157, 180, 568, 639, 741, 889, 1046
DpnII GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
EaeI YGGCCR 1 cut(s) 521
Eam1104I CTCTTC 2 cut(s) 107, 1293
EarI CTCTTC 2 cut(s) 107, 1293
Eco130I CCWWGG 1 cut(s) 21
Eco72I CACGTG 1 cut(s) 1241
Eco81I CCTNAGG 1 cut(s) 48
EcoRII CCWGG 5 cut(s) 73, 936, 947, 1216, 1469
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 8 cut(s) 25, 377, 403, 784, 830, 851, 1254, 1403
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FaqI GGGAC 1 cut(s) 1135
FatI CATG 8 cut(s) 21, 373, 399, 780, 826, 847, 1250, 1399
FauNDI CATATG 1 cut(s) 763
FbaI TGATCA 1 cut(s) 178
FokI GGATG 4 cut(s) 382, 494, 1168, 1520
FspBI CTAG 6 cut(s) 579, 933, 1017, 1098, 1158, 1284
GlaI GCGC 2 cut(s) 313, 1396
GsaI CCCAGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 931
HaeIII GGCC 4 cut(s) 276, 523, 1006, 1469
HapII CCGG 4 cut(s) 295, 520, 1480, 1527
HgaI GACGC 1 cut(s) 1401
HhaI GCGC 2 cut(s) 314, 1397
Hin1II CATG 8 cut(s) 25, 377, 403, 784, 830, 851, 1254, 1403
Hin6I GCGC 2 cut(s) 312, 1395
HinP1I GCGC 2 cut(s) 312, 1395
HindIII AAGCTT 3 cut(s) 1032, 1314, 1406
HinfI GANTC 8 cut(s) 131, 137, 266, 758, 803, 830, 982, 1054
HpaII CCGG 4 cut(s) 295, 520, 1480, 1527
HphI GGTGA 3 cut(s) 3, 63, 1029
Hpy166II GTNNAC 1 cut(s) 602
Hpy188I TCNGA 9 cut(s) 136, 155, 202, 414, 492, 800, 1044, 1075, 1332
Hpy188III TCNNGA 6 cut(s) 167, 327, 570, 635, 1445, 1569
Hpy8I GTNNAC 1 cut(s) 602
HpyAV CCTTC 8 cut(s) 27, 112, 298, 360, 409, 885, 1072, 1327
HpyCH4III ACNGT 3 cut(s) 209, 471, 1128
HpyCH4IV ACGT 2 cut(s) 1187, 1240
HpyCH4V TGCA 8 cut(s) 143, 380, 547, 1028, 1181, 1202, 1244, 1430
HpyF10VI GCNNNNNNNGC 8 cut(s) 425, 448, 553, 1031, 1154, 1268, 1401, 1466
HpySE526I ACGT 2 cut(s) 1187, 1240
Hsp92II CATG 8 cut(s) 25, 377, 403, 784, 830, 851, 1254, 1403
HspAI GCGC 2 cut(s) 312, 1395
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
LweI GCATC 5 cut(s) 323, 367, 475, 1190, 1542
MaeI CTAG 6 cut(s) 579, 933, 1017, 1098, 1158, 1284
MaeII ACGT 2 cut(s) 1187, 1240
MaeIII GTNAC 3 cut(s) 290, 1083, 1188
MalI GATC 8 cut(s) 38, 157, 180, 568, 639, 741, 889, 1046
MboI GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
MboII GAAGA 9 cut(s) 121, 124, 227, 292, 356, 671, 825, 1220, 1310
MfeI CAATTG 1 cut(s) 605
MflI RGATCY 3 cut(s) 36, 155, 887
MhlI GDGCHC 4 cut(s) 430, 614, 780, 1568
MluCI AATT 5 cut(s) 322, 330, 605, 678, 997
MlyI GAGTC 2 cut(s) 260, 812
MmeI TCCRAC 2 cut(s) 544, 778
MspA1I CMGCKG 2 cut(s) 1427, 1463
MspI CCGG 4 cut(s) 295, 520, 1480, 1527
MspR9I CCNGG 5 cut(s) 75, 938, 949, 1218, 1471
MunI CAATTG 1 cut(s) 605
Mva1269I GAATGC 2 cut(s) 143, 262
MvaI CCWGG 5 cut(s) 75, 938, 949, 1218, 1471
MvnI CGCG 2 cut(s) 571, 1395
MwoI GCNNNNNNNGC 8 cut(s) 425, 448, 553, 1031, 1154, 1268, 1401, 1466
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 763
NdeII GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
NheI GCTAGC 2 cut(s) 932, 1157
NlaIII CATG 8 cut(s) 25, 377, 403, 784, 830, 851, 1254, 1403
NlaIV GGNNCC 4 cut(s) 38, 338, 441, 942
NmuCI GTSAC 2 cut(s) 290, 1188
NruI TCGCGA 1 cut(s) 571
NspV TTCGAA 2 cut(s) 254, 515
PctI GAATGC 2 cut(s) 143, 262
PfeI GAWTC 6 cut(s) 131, 137, 758, 830, 982, 1054
PfoI TCCNGGA 1 cut(s) 947
PinAI ACCGGT 1 cut(s) 1526
PleI GAGTC 2 cut(s) 260, 811
PmaCI CACGTG 1 cut(s) 1241
PmlI CACGTG 1 cut(s) 1241
PpsI GAGTC 2 cut(s) 260, 811
Ppu21I YACGTR 1 cut(s) 1241
Psp6I CCWGG 5 cut(s) 73, 936, 947, 1216, 1469
PspCI CACGTG 1 cut(s) 1241
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 5 cut(s) 73, 936, 947, 1216, 1469
PspN4I GGNNCC 4 cut(s) 38, 338, 441, 942
PspPI GGNCC 1 cut(s) 275
PstNI CAGNNNCTG 1 cut(s) 1349
PsuI RGATCY 3 cut(s) 36, 155, 887
PvuII CAGCTG 1 cut(s) 1427
RruI TCGCGA 1 cut(s) 571
RsaI GTAC 2 cut(s) 913, 1514
RsaNI GTAC 2 cut(s) 912, 1513
Sau3AI GATC 8 cut(s) 36, 155, 178, 566, 637, 739, 887, 1044
Sau96I GGNCC 1 cut(s) 275
SchI GAGTC 2 cut(s) 260, 812
ScrFI CCNGG 5 cut(s) 75, 938, 949, 1218, 1471
SduI GDGCHC 4 cut(s) 430, 614, 780, 1568
SfaNI GCATC 5 cut(s) 323, 367, 475, 1190, 1542
SfcI CTRYAG 1 cut(s) 837
SfuI TTCGAA 2 cut(s) 254, 515
SmlI CTYRAG 1 cut(s) 1443
SmoI CTYRAG 1 cut(s) 1443
Sse9I AATT 5 cut(s) 322, 330, 605, 678, 997
SsiI CCGC 2 cut(s) 882, 1463
SspMI CTAG 6 cut(s) 579, 933, 1017, 1098, 1158, 1284
StyD4I CCNGG 5 cut(s) 73, 936, 947, 1216, 1469
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 3 cut(s) 209, 471, 1128
TaiI ACGT 2 cut(s) 1190, 1243
TaqI TCGA 4 cut(s) 248, 254, 515, 1377
TasI AATT 5 cut(s) 322, 330, 605, 678, 997
TatI WGTACW 1 cut(s) 1512
TfiI GAWTC 6 cut(s) 131, 137, 758, 830, 982, 1054
TscAI CASTG 2 cut(s) 780, 1209
TseFI GTSAC 2 cut(s) 290, 1188
Tsp45I GTSAC 2 cut(s) 290, 1188
TspDTI ATGAA 7 cut(s) 342, 750, 836, 1106, 1154, 1319, 1427
TspRI CASTG 2 cut(s) 780, 1209
XspI CTAG 6 cut(s) 579, 933, 1017, 1098, 1158, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.