MD13G1018900.v1.1

Homeobox protein knotted-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002850
Physical Location & Seq
Reverse (-)
1182375 .. 1182962
588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1018900.v1.1.491

Sequence Viewer

Length: 330 bp
ATGGTGGGTTTGGGTGAAATTGGAGATCAAATCGGTGATAAAAGCACTACTACAACGGACAATAACATTGTCAATGCCAAGCAATTCATCAAGGACGCTACCAGCAGCGATCGCGATTCTTCAAACCTTGATCAGTTCATGGAAGCGTACTGCAACGGTCTAAACAAGCTCAAAGAAGCCATGGCGGATCCCATGAAGGAAATGACATCTTTTATCACTGCTATGTATGCTCAACTTGAGGACCTCAGTGGTTCAAAAAAAAAAACCATTACCCACCCGGCCTGCTGGCGGCGATCTGGCTATGTCGTCTGCGTTTCAGCCGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KNOX1 KNOX2

Protein Analysis

110

Amino Acids

12.0

Weight (kDa)

4.95

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KNOX2 PF03791 41 - 82 4.1e-10 KNOX2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 114
AciI CCGC 2 cut(s) 185, 289
AclWI GGATC 2 cut(s) 182, 195
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 2 cut(s) 123, 255
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
AlwI GGATC 2 cut(s) 182, 195
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 105
AsiSI GCGATCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 241
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 26, 47
AvaII GGWCC 1 cut(s) 241
BamHI GGATCC 1 cut(s) 187
BbvI GCAGC 1 cut(s) 117
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 278
BisI GCNGC 2 cut(s) 106, 290
BlsI GCNGC 2 cut(s) 107, 291
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 180, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 180, 321
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 288
BsnI GGCC 1 cut(s) 281
Bsp143I GATC 5 cut(s) 25, 109, 130, 187, 293
Bsp19I CCATGG 1 cut(s) 180
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 2 cut(s) 185, 289
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 1 cut(s) 258
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 2 cut(s) 182, 195
BssECI CCNNGG 2 cut(s) 180, 321
BssMI GATC 5 cut(s) 25, 109, 130, 187, 293
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 283, 287
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 1 cut(s) 180
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 5 cut(s) 28, 112, 133, 190, 296
BstMBI GATC 5 cut(s) 25, 109, 130, 187, 293
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 111, 227
BstSCI CCNGG 1 cut(s) 276
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 216, 253
BtuMI TCGCGA 1 cut(s) 114
Cac8I GCNNGC 2 cut(s) 283, 287
Cfr13I GGNCC 1 cut(s) 241
CseI GACGC 1 cut(s) 104
Csp6I GTAC 1 cut(s) 148
CviAII CATG 3 cut(s) 139, 181, 193
CviJI RGCY 5 cut(s) 169, 179, 281, 300, 320
CviKI_1 RGCY 5 cut(s) 169, 179, 281, 300, 320
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 245
DpnI GATC 5 cut(s) 27, 111, 132, 189, 295
DpnII GATC 5 cut(s) 25, 109, 130, 187, 293
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 3 cut(s) 142, 184, 196
FaiI YATR 6 cut(s) 140, 182, 194, 224, 228, 303
FatI CATG 3 cut(s) 138, 180, 192
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 106, 290
Fsp4HI GCNGC 2 cut(s) 106, 290
GluI GCNGC 2 cut(s) 106, 290
HaeIII GGCC 1 cut(s) 281
HapII CCGG 1 cut(s) 278
HgaI GACGC 1 cut(s) 104
Hin1II CATG 3 cut(s) 142, 184, 196
HinfI GANTC 1 cut(s) 116
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 26, 47
Hpy188III TCNNGA 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 153
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 227
HpyF3I CTNAG 1 cut(s) 245
Hsp92II CATG 3 cut(s) 142, 184, 196
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 5 cut(s) 25, 109, 130, 187, 293
LpnPI CCDG 5 cut(s) 115, 271, 282, 291, 295
Lsp1109I GCAGC 1 cut(s) 117
MalI GATC 5 cut(s) 27, 111, 132, 189, 295
MboI GATC 5 cut(s) 25, 109, 130, 187, 293
MboII GAAGA 1 cut(s) 111
MflI RGATCY 1 cut(s) 187
MluCI AATT 2 cut(s) 18, 83
MnlI CCTC 3 cut(s) 232, 254, 316
MslI CAYNNNNRTG 1 cut(s) 221
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 2 cut(s) 111, 227
NciI CCSGG 1 cut(s) 278
NcoI CCATGG 1 cut(s) 180
NdeII GATC 5 cut(s) 25, 109, 130, 187, 293
NlaIII CATG 3 cut(s) 142, 184, 196
NlaIV GGNNCC 1 cut(s) 189
NruI TCGCGA 1 cut(s) 114
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 2 cut(s) 107, 291
Ple19I CGATCG 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 241
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 1 cut(s) 187
PvuI CGATCG 1 cut(s) 112
RgaI GCGATCGC 1 cut(s) 112
RruI TCGCGA 1 cut(s) 114
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 1 cut(s) 221
SatI GCNGC 2 cut(s) 106, 290
Sau3AI GATC 5 cut(s) 25, 109, 130, 187, 293
Sau96I GGNCC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 278
SetI ASST 3 cut(s) 129, 171, 246
SfaAI GCGATCGC 1 cut(s) 112
SgfI GCGATCGC 1 cut(s) 112
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 2 cut(s) 18, 83
SsiI CCGC 2 cut(s) 185, 289
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 158
TasI AATT 2 cut(s) 18, 83
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 1 cut(s) 116
TscAI CASTG 2 cut(s) 223, 253
TseI GCWGC 1 cut(s) 105
TspDTI ATGAA 3 cut(s) 76, 127, 209
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 2 cut(s) 223, 253
VpaK11BI GGWCC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.