RLG00000005445

Homeobox protein knotted-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002850
Physical Location & Seq
Forward (+)
67691679 .. 67693125
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005445

Sequence Viewer

Length: 501 bp
ATGAACAAATATGGCAACAGAGTCACGAGGAGGAAGAAAGTGAAGAAGATTCCCATCAATGCCATCAATCTCAAACGGAGAATCTCCAGTCATTCTCTGTATGAGCGATTGGTATACACAATCACTTTGGAGTTCTTGAAGGCCGTCGGGTTTCATTGTGAGTTTGCTGACCTAGAGAGAAATGATAGCCAAGAAGTAAAACAGGCGTTAGGCTTGCCTACTCAACAAAAAGAGCTAGATCAGTTCATGGAAGCATACCGCTTGGCACTTGGGAAGTTGAAAGAAGCAATGGAGGAACCTTACCAGATCTCCATGGCTTTCATAACAAACATGCATCTCCAACTCGAGGAGCAAGCATGCTTCTCCCATCCACATGAACCTGCAGACGCTCTTCTGCCCAGCCCAATGGACTTGGTTCCAGCTCATAAGCTTAATAACTCTAAGAGCCCTAACGGCCCAATAGATCGAAAGATCCCAAATGGACTTGATTCCAGCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KNOX1 KNOX2

Protein Analysis

167

Amino Acids

18.97

Weight (kDa)

8.83

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KNOX2 PF03791 75 - 116 2.5e-06 KNOX2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 388
AccI GTMKAC 1 cut(s) 114
AciI CCGC 1 cut(s) 259
AclWI GGATC 1 cut(s) 466
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 2 cut(s) 139, 280
AluBI AGCT 3 cut(s) 235, 422, 430
AluI AGCT 3 cut(s) 235, 422, 430
AlwI GGATC 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 2 cut(s) 141, 454
AspS9I GGNCC 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 344
BanII GRGCYC 1 cut(s) 449
BauI CACGAG 1 cut(s) 25
BccI CCATC 3 cut(s) 62, 71, 375
BceAI ACGGC 2 cut(s) 128, 469
BfaI CTAG 2 cut(s) 173, 236
BfmI CTRYAG 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 388
BglI GCCNNNNNGGC 1 cut(s) 453
BglII AGATCT 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 297, 417
BmsI GCATC 1 cut(s) 343
BpmI CTGGAG 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 312
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 1 cut(s) 367
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 2 cut(s) 43, 362
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 2 cut(s) 143, 456
BsiHKCI CYCGRG 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 346
BsnI GGCC 2 cut(s) 143, 456
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 4 cut(s) 238, 306, 463, 471
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 1 cut(s) 259
BspANI GGCC 2 cut(s) 143, 456
BspLI GGNNCC 2 cut(s) 297, 417
BspMAI CTGCAG 1 cut(s) 385
BspMI ACCTGC 1 cut(s) 388
BspPI GGATC 1 cut(s) 466
BspQI GCTCTTC 1 cut(s) 396
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 4 cut(s) 238, 306, 463, 471
BssNAI GTATAC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 312
Bst1107I GTATAC 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 396
BstC8I GCNNGC 3 cut(s) 215, 354, 358
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 367
BstKTI GATC 4 cut(s) 241, 309, 466, 474
BstMBI GATC 4 cut(s) 238, 306, 463, 471
BstMWI GCNNNNNNNGC 1 cut(s) 453
BstNSI RCATGY 2 cut(s) 334, 360
BstSFI CTRYAG 1 cut(s) 381
BstX2I RGATCY 2 cut(s) 306, 471
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 2 cut(s) 306, 471
BstZ17I GTATAC 1 cut(s) 115
BsuRI GGCC 2 cut(s) 143, 456
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 367
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 3 cut(s) 215, 354, 358
Cfr13I GGNCC 1 cut(s) 455
CseI GACGC 1 cut(s) 395
CviAII CATG 5 cut(s) 247, 313, 331, 357, 374
DdeI CTNAG 1 cut(s) 441
DpnI GATC 4 cut(s) 240, 308, 465, 473
DpnII GATC 4 cut(s) 238, 306, 463, 471
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 312
Eco24I GRGCYC 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 312
EcoT22I ATGCAT 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 5 cut(s) 250, 316, 334, 360, 377
FatI CATG 5 cut(s) 246, 312, 330, 356, 373
FblI GTMKAC 1 cut(s) 114
FokI GGATG 1 cut(s) 354
FriOI GRGCYC 1 cut(s) 449
FspBI CTAG 2 cut(s) 173, 236
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 2 cut(s) 143, 456
HgaI GACGC 1 cut(s) 395
Hin1II CATG 5 cut(s) 250, 316, 334, 360, 377
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 4 cut(s) 21, 49, 81, 488
Hpy166II GTNNAC 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 25, 136
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 1 cut(s) 133
HpyCH4V TGCA 2 cut(s) 334, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 453
HpyF3I CTNAG 1 cut(s) 441
Hsp92II CATG 5 cut(s) 250, 316, 334, 360, 377
Kzo9I GATC 4 cut(s) 238, 306, 463, 471
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 6 cut(s) 100, 188, 317, 393, 412, 432
LweI GCATC 1 cut(s) 343
MaeI CTAG 2 cut(s) 173, 236
MaeIII GTNAC 1 cut(s) 22
MalI GATC 4 cut(s) 240, 308, 465, 473
MboI GATC 4 cut(s) 238, 306, 463, 471
MboII GAAGA 4 cut(s) 46, 55, 58, 383
MflI RGATCY 2 cut(s) 306, 471
MhlI GDGCHC 1 cut(s) 449
MlyI GAGTC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 4 cut(s) 21, 24, 286, 340
Mph1103I ATGCAT 1 cut(s) 336
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 453
NcoI CCATGG 1 cut(s) 312
NdeII GATC 4 cut(s) 238, 306, 463, 471
NlaIII CATG 5 cut(s) 250, 316, 334, 360, 377
NlaIV GGNNCC 2 cut(s) 297, 417
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 2 cut(s) 334, 360
PaeI GCATGC 1 cut(s) 360
PaeR7I CTCGAG 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 396
PfeI GAWTC 3 cut(s) 49, 81, 488
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 398
PspN4I GGNNCC 2 cut(s) 297, 417
PspPI GGNCC 1 cut(s) 455
PspXI VCTCGAGB 1 cut(s) 344
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 2 cut(s) 306, 471
RseI CAYNNNNRTG 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 432
Sau3AI GATC 4 cut(s) 238, 306, 463, 471
Sau96I GGNCC 1 cut(s) 455
SchI GAGTC 1 cut(s) 30
SduI GDGCHC 1 cut(s) 449
SetI ASST 6 cut(s) 174, 237, 301, 382, 424, 432
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 381
Sfr274I CTCGAG 1 cut(s) 344
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SphI GCATGC 1 cut(s) 360
SsiI CCGC 1 cut(s) 259
SspMI CTAG 2 cut(s) 173, 236
StyI CCWWGG 1 cut(s) 312
TaqI TCGA 2 cut(s) 345, 466
TfiI GAWTC 3 cut(s) 49, 81, 488
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 5 cut(s) 17, 143, 235, 310, 390
TspGWI ACGGA 1 cut(s) 91
XceI RCATGY 2 cut(s) 334, 360
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 2 cut(s) 173, 236
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.