MD13G1035200.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010081
Physical Location & Seq
Forward (+)
2470384 .. 2471575
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1035200.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGATCCAAATGCATTAAGCTTTCTGGTCAGTCCTTCAACCTTCCAGTCAGCTTACTACGAAGCCAAAGAGAATGATGGCATCATTGATCTTGGTCTTAGCCTCAGAGCTCTGCAGCCTGAAACTTACCATCCATCTGCACATTTGGTGAGCTTGGAGGGATATGATGATCTGATAGATTGGCCCCAGGCTAACCTGAACTTGAAGAACACTAGCATTCTAAACGTGAGAAGTGTCGCGGAAGATTGCGACGAAGAAGCAGAGGGAGTGCAAAGCAAGGAGAGGAGGGCTTATGTGAAGGTTAACATGGATGGGCTTGTTATTGGCAGGAAAGTCTGTATTCCTGATCACAGTGGTTACTCGAGTCTTGCATTTCAGCTGGAAGACATGTTTGGTAGACAATCTGGATCTGGGTTAAGGTTATTTCAGGATGGGTCCGAGTTTTTGCTTTTTTACAAGGACAGAGATGACAATTGGCGAACTGTCGGTGATGTTCCATGGAAGGAATTCGTAGAATGTGTGACGCGAATGAGGATTGCAAAAAAGAATCAAGCTTTTCTTTCACCTCCTTTGAAATTTAACTAA

Protein Analysis

195

Amino Acids

21.98

Weight (kDa)

4.92

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 60 - 185 1.1e-22 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15050 AT1G15050 AT2G01200 AT2G01200
fragaria_vesca FvH4_4g26700
malus_domestica MD13G1035200.v1.1
prunus_persica Prupe.1G317000_v2.0.a1
pyrus_communis pycom13g03080
rosa_chinensis RchiOBHm_Chr4g0434391
rosa_laevigata RLG00000006675
rosa_multiflora Rmu_co8521181.1_g000002
rosa_roxburghii Rroxscaffold_5G00375750
rosa_rugosa Rorug02G0288600 Rorug03G0339700 Rorug04G0276300
rosa_samantha Rh4AG331800 Rh4BG339600 Rh4CG354800 Rh4DG040900 Rh4DG334800
rosa_wichuraiana Rw4G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 397
AccII CGCG 2 cut(s) 239, 526
AciI CCGC 1 cut(s) 239
AclWI GGATC 2 cut(s) 12, 415
AcsI RAATTY 2 cut(s) 506, 575
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 3 cut(s) 39, 205, 574
AjnI CCWGG 1 cut(s) 186
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AluBI AGCT 6 cut(s) 21, 53, 110, 153, 379, 554
AluI AGCT 6 cut(s) 21, 53, 110, 153, 379, 554
Alw21I GWGCWC 1 cut(s) 112
AlwI GGATC 2 cut(s) 12, 415
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 2 cut(s) 506, 575
Asp700I GAANNNNTTC 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 183, 435
AsuHPI GGTGA 3 cut(s) 160, 500, 555
AvaI CYCGRG 1 cut(s) 361
AvaII GGWCC 1 cut(s) 435
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 112
BbvI GCAGC 1 cut(s) 127
BccI CCATC 5 cut(s) 71, 138, 142, 305, 425
BciT130I CCWGG 1 cut(s) 188
BclI TGATCA 1 cut(s) 346
BfaI CTAG 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 113
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 435
BmeT110I CYCGRG 1 cut(s) 361
BmgT120I GGNCC 2 cut(s) 183, 435
BmiI GGNNCC 3 cut(s) 6, 185, 436
BmrFI CCNGG 1 cut(s) 188
BmsI GCATC 1 cut(s) 90
BpiI GAAGAC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 186, 497
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 186, 497
BseGI GGATG 3 cut(s) 130, 316, 436
BseMII CTCAG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 298
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 239, 526
BshFI GGCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 361
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 5 cut(s) 4, 88, 169, 346, 407
Bsp19I CCATGG 1 cut(s) 497
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 117
BspFNI CGCG 2 cut(s) 239, 526
BspLI GGNNCC 3 cut(s) 6, 185, 436
BspMAI CTGCAG 1 cut(s) 117
BspPI GGATC 2 cut(s) 12, 415
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 186, 497
BssMI GATC 5 cut(s) 4, 88, 169, 346, 407
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 2 cut(s) 353, 484
BstDEI CTNAG 2 cut(s) 98, 104
BstDSI CCRYGG 1 cut(s) 497
BstF5I GGATG 3 cut(s) 130, 316, 436
BstFNI CGCG 2 cut(s) 239, 526
BstKTI GATC 5 cut(s) 7, 91, 172, 349, 410
BstMBI GATC 5 cut(s) 4, 88, 169, 346, 407
BstNI CCWGG 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 113
BstUI CGCG 2 cut(s) 239, 526
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 2 cut(s) 4, 407
BstYI RGATCY 2 cut(s) 4, 407
BsuRI GGCC 1 cut(s) 184
BtgI CCRYGG 1 cut(s) 497
BtsCI GGATG 3 cut(s) 130, 316, 436
BtsIMutI CAGTG 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 183, 435
CseI GACGC 1 cut(s) 532
CviAII CATG 3 cut(s) 307, 388, 498
DdeI CTNAG 2 cut(s) 98, 104
DpnI GATC 5 cut(s) 6, 90, 171, 348, 409
DpnII GATC 5 cut(s) 4, 88, 169, 346, 407
Ecl136II GAGCTC 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 497
Eco24I GRGCYC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 3 cut(s) 310, 391, 501
FaiI YATR 5 cut(s) 165, 294, 308, 389, 499
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FatI CATG 3 cut(s) 306, 387, 497
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 3 cut(s) 117, 323, 443
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 1 cut(s) 213
GluI GCNGC 1 cut(s) 116
HaeIII GGCC 1 cut(s) 184
HgaI GACGC 1 cut(s) 532
Hin1II CATG 3 cut(s) 310, 391, 501
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 2 cut(s) 19, 552
HinfI GANTC 2 cut(s) 364, 547
HpaI GTTAAC 1 cut(s) 304
HphI GGTGA 3 cut(s) 160, 500, 555
Hpy166II GTNNAC 2 cut(s) 304, 398
Hpy188I TCNGA 3 cut(s) 107, 174, 439
Hpy188III TCNNGA 3 cut(s) 344, 405, 428
Hpy8I GTNNAC 2 cut(s) 304, 398
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 4 cut(s) 45, 52, 292, 496
HpyCH4III ACNGT 2 cut(s) 353, 484
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 6 cut(s) 14, 115, 140, 271, 371, 539
HpyF3I CTNAG 2 cut(s) 98, 104
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 3 cut(s) 310, 391, 501
Ksp22I TGATCA 1 cut(s) 346
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 5 cut(s) 4, 88, 169, 346, 407
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 90
MaeI CTAG 1 cut(s) 213
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 356, 520
MalI GATC 5 cut(s) 6, 90, 171, 348, 409
MboI GATC 5 cut(s) 4, 88, 169, 346, 407
MboII GAAGA 4 cut(s) 217, 254, 266, 395
MfeI CAATTG 1 cut(s) 472
MflI RGATCY 2 cut(s) 4, 407
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 3 cut(s) 472, 506, 575
MlyI GAGTC 1 cut(s) 373
MnlI CCTC 7 cut(s) 113, 151, 256, 276, 279, 525, 576
Mph1103I ATGCAT 1 cut(s) 16
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 4 cut(s) 17, 303, 416, 579
MspA1I CMGCKG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 188
MunI CAATTG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 2 cut(s) 239, 526
NcoI CCATGG 1 cut(s) 497
NdeII GATC 5 cut(s) 4, 88, 169, 346, 407
NlaIII CATG 3 cut(s) 310, 391, 501
NlaIV GGNNCC 3 cut(s) 6, 185, 436
NmuCI GTSAC 1 cut(s) 520
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 1 cut(s) 391
PaeR7I CTCGAG 1 cut(s) 361
PciI ACATGT 1 cut(s) 387
PctI GAATGC 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 547
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PscI ACATGT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 3 cut(s) 6, 185, 436
PspPI GGNCC 2 cut(s) 183, 435
PspXI VCTCGAGB 1 cut(s) 361
PstI CTGCAG 1 cut(s) 117
PsuI RGATCY 2 cut(s) 4, 407
PvuII CAGCTG 1 cut(s) 379
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 4 cut(s) 17, 303, 416, 579
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 5 cut(s) 4, 88, 169, 346, 407
Sau96I GGNCC 2 cut(s) 183, 435
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 112
SfaNI GCATC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 113
Sfr274I CTCGAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 435
SlaI CTCGAG 1 cut(s) 361
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 3 cut(s) 472, 506, 575
SsiI CCGC 1 cut(s) 239
SspMI CTAG 1 cut(s) 213
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 2 cut(s) 353, 484
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 362
TasI AATT 3 cut(s) 472, 506, 575
TfiI GAWTC 1 cut(s) 547
Tru1I TTAA 4 cut(s) 17, 303, 416, 579
Tru9I TTAA 4 cut(s) 17, 303, 416, 579
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 520
TspRI CASTG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 2 cut(s) 506, 575
XceI RCATGY 1 cut(s) 391
XhoI CTCGAG 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 397
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 1 cut(s) 213
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.