Rw4G028770

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010081
Physical Location & Seq
Forward (+)
55388709 .. 55389793
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028770.1

Sequence Viewer

Length: 387 bp
ATGGATCCTAATGCATCAAGCTCATTTTATTATGAAGCCAAAGAGAATGAGGGTATTATTGATCTTGGTCTTAGCCTCAGAGCTCTTCAACCTGAAACTTATCATCATCCATCAACACATTTGGTGAGCTTGGAGGGCTATGATGGTCTGATAGATTGGCCCCGGGCTAACAACTTGAATTTGAAGAATTCGAATGTCATTAGTCCGAGAAATATACCGGAAGATTGTGATGAGGAAGCAGAGGGAGTCCAGAGCAAGGAGAGGTGGGCTTATGTGAAAGTTAACATGGATGGGATTGTTATTGGCAGAAAAGTCTGTGTAATTGATCATAGTGGCTACTCGAGCCTTGCATTTCAACTAGAAGATATGTTTGGTAGGTGCAATTAA

Protein Analysis

128

Amino Acids

14.41

Weight (kDa)

4.75

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 77 - 125 4.8e-10 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15050 AT1G15050 AT2G01200 AT2G01200
fragaria_vesca FvH4_4g26700
malus_domestica MD13G1035200.v1.1
prunus_persica Prupe.1G317000_v2.0.a1
pyrus_communis pycom13g03080
rosa_chinensis RchiOBHm_Chr4g0434391
rosa_laevigata RLG00000006675
rosa_multiflora Rmu_co8521181.1_g000002
rosa_roxburghii Rroxscaffold_5G00375750
rosa_rugosa Rorug02G0288600 Rorug03G0339700 Rorug04G0276300
rosa_samantha Rh4AG331800 Rh4BG339600 Rh4CG354800 Rh4DG040900 Rh4DG334800
rosa_wichuraiana Rw4G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 178, 187
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 4 cut(s) 89, 178, 184, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 3 cut(s) 21, 83, 129
AluI AGCT 3 cut(s) 21, 83, 129
Alw21I GWGCWC 1 cut(s) 85
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 2 cut(s) 162, 340
AoxI GGCC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 178, 187
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 2 cut(s) 163, 164
AsuHPI GGTGA 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 191
AvaI CYCGRG 2 cut(s) 162, 340
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 85
BccI CCATC 3 cut(s) 118, 137, 284
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 2 cut(s) 163, 164
BfaI CTAG 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 163, 164
BmeT110I CYCGRG 2 cut(s) 162, 340
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 6, 161
BmrFI CCNGG 2 cut(s) 163, 164
BmsI GCATC 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 191
BpuMI CCSGG 2 cut(s) 163, 164
BsaJI CCNNGG 2 cut(s) 161, 162
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 256
BseDI CCNNGG 2 cut(s) 161, 162
BseGI GGATG 2 cut(s) 106, 295
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 91
BshFI GGCC 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 2 cut(s) 162, 340
BsiSI CCGG 2 cut(s) 163, 218
BslI CCNNNNNNNGG 1 cut(s) 256
BsnI GGCC 1 cut(s) 160
BsoBI CYCGRG 2 cut(s) 162, 340
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 3 cut(s) 4, 61, 325
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 6, 161
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 90
BspT104I TTCGAA 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 161, 162
BssMI GATC 3 cut(s) 4, 61, 325
Bst6I CTCTTC 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 191
BstDEI CTNAG 2 cut(s) 71, 77
BstF5I GGATG 2 cut(s) 106, 295
BstKTI GATC 3 cut(s) 7, 64, 328
BstMBI GATC 3 cut(s) 4, 61, 325
BstMWI GCNNNNNNNGC 2 cut(s) 135, 342
BstSCI CCNGG 2 cut(s) 161, 162
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 160
BtsCI GGATG 2 cut(s) 106, 295
Cfr13I GGNCC 1 cut(s) 159
Cfr9I CCCGGG 1 cut(s) 162
CviAII CATG 1 cut(s) 286
DdeI CTNAG 2 cut(s) 71, 77
DpnI GATC 3 cut(s) 6, 63, 327
DpnII GATC 3 cut(s) 4, 61, 325
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 162, 340
EcoICRI GAGCTC 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 85
FaeI CATG 1 cut(s) 289
FaiI YATR 7 cut(s) 33, 141, 215, 273, 287, 330, 368
FatI CATG 1 cut(s) 285
FbaI TGATCA 1 cut(s) 325
FokI GGATG 2 cut(s) 93, 302
FriOI GRGCYC 1 cut(s) 85
FspBI CTAG 1 cut(s) 359
HaeIII GGCC 1 cut(s) 160
HapII CCGG 2 cut(s) 163, 218
Hin1II CATG 1 cut(s) 289
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 1 cut(s) 246
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 163, 218
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 3 cut(s) 80, 150, 207
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 1 cut(s) 283
HpyCH4V TGCA 3 cut(s) 14, 350, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 342
HpyF3I CTNAG 2 cut(s) 71, 77
Hsp92II CATG 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 325
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 3 cut(s) 4, 61, 325
LguI GCTCTTC 1 cut(s) 90
LpnPI CCDG 4 cut(s) 105, 176, 231, 263
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 359
MalI GATC 3 cut(s) 6, 63, 327
MboI GATC 3 cut(s) 4, 61, 325
MboII GAAGA 4 cut(s) 77, 196, 233, 374
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 4 cut(s) 178, 187, 321, 382
MlyI GAGTC 1 cut(s) 255
MnlI CCTC 6 cut(s) 43, 86, 127, 226, 235, 255
Mph1103I ATGCAT 1 cut(s) 16
MseI TTAA 2 cut(s) 282, 385
MspI CCGG 2 cut(s) 163, 218
MspR9I CCNGG 2 cut(s) 163, 164
MwoI GCNNNNNNNGC 2 cut(s) 135, 342
NciI CCSGG 2 cut(s) 163, 164
NdeII GATC 3 cut(s) 4, 61, 325
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 2 cut(s) 6, 161
NsiI ATGCAT 1 cut(s) 16
NspV TTCGAA 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 90
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 6, 161
PspPI GGNCC 1 cut(s) 159
PspXI VCTCGAGB 1 cut(s) 340
PsuI RGATCY 1 cut(s) 4
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 90
SaqAI TTAA 2 cut(s) 282, 385
Sau3AI GATC 3 cut(s) 4, 61, 325
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 2 cut(s) 163, 164
SduI GDGCHC 1 cut(s) 85
SetI ASST 6 cut(s) 23, 85, 94, 131, 266, 380
SfaNI GCATC 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 340
SfuI TTCGAA 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 340
SmaI CCCGGG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 4 cut(s) 178, 187, 321, 382
SspMI CTAG 1 cut(s) 359
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 2 cut(s) 161, 162
TaqI TCGA 2 cut(s) 191, 341
TasI AATT 4 cut(s) 178, 187, 321, 382
Tru1I TTAA 2 cut(s) 282, 385
Tru9I TTAA 2 cut(s) 282, 385
TspDTI ATGAA 1 cut(s) 48
TspMI CCCGGG 1 cut(s) 162
XapI RAATTY 2 cut(s) 178, 187
XhoI CTCGAG 1 cut(s) 340
XmaI CCCGGG 1 cut(s) 162
XspI CTAG 1 cut(s) 359
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.