Rh4CG354800

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010081
Physical Location & Seq
Forward (+)
61089957 .. 61091522
1566 bp
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UTR
Exon/CDS
Intron
Rh4CG354800.1

Sequence Viewer

Length: 582 bp
ATGGATCCTAATGCATCAAGCTCTCTTCTGAACCCTTCAAGCTTTCAATCATTTTATTATGAAGCTAAAGAGAGTGAGGGTATTATTGATCTTGGTCTTAGCCTCAGAGCTCTTCAACCTGAAACTTATCATCATCCATCAGCACATTTGGTGAGCTTGGAGGGCTATGATGGTCTGATAGATTGGCCCCAGGCTAACAACTTGAATTTGAAGAATTCAAATGTCACAAGTCCGAGAAATATACCGGAAGATTGTGATGAGGAAGCAGAGGGAGTCCAGAGCAAGGAGAGGTGGGCTTATGTGAAAGTTAACATGGATGGGATTGTTATTGGCAGAAAAGTTTGTGTAATTGATCATAGTGGCTACTCGAGCCTTGCATTTCAACTAGAAGACATGTTTGGTAGACAATCTGTATCTGGGTTGAGGTTGTTCCAGGTTGGATCTGAGTTTTCACTTTTTTACAAGGACAGAGATGACAATTGGAGAACTGTTGGTGACGTTCCATGGAGGTACATGCTCCTTATCTACGACTTAAATTCTCCACTGTCATTTTCCATTTTATTTGTTCATTTATTATCTTAG

Protein Analysis

193

Amino Acids

21.89

Weight (kDa)

4.76

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 72 - 171 6.9e-16 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15050 AT1G15050 AT2G01200 AT2G01200
fragaria_vesca FvH4_4g26700
malus_domestica MD13G1035200.v1.1
prunus_persica Prupe.1G317000_v2.0.a1
pyrus_communis pycom13g03080
rosa_chinensis RchiOBHm_Chr4g0434391
rosa_laevigata RLG00000006675
rosa_multiflora Rmu_co8521181.1_g000002
rosa_roxburghii Rroxscaffold_5G00375750
rosa_rugosa Rorug02G0288600 Rorug03G0339700 Rorug04G0276300
rosa_samantha Rh4AG331800 Rh4BG339600 Rh4CG354800 Rh4DG040900 Rh4DG334800
rosa_wichuraiana Rw4G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 403
AclWI GGATC 2 cut(s) 12, 448
AcsI RAATTY 3 cut(s) 205, 214, 535
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 7 cut(s) 39, 47, 116, 205, 211, 219, 383
AjnI CCWGG 2 cut(s) 189, 432
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AluBI AGCT 5 cut(s) 21, 42, 65, 110, 156
AluI AGCT 5 cut(s) 21, 42, 65, 110, 156
Alw21I GWGCWC 1 cut(s) 112
AlwI GGATC 2 cut(s) 12, 448
Ama87I CYCGRG 1 cut(s) 367
AoxI GGCC 1 cut(s) 185
ApoI RAATTY 3 cut(s) 205, 214, 535
AspS9I GGNCC 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 163, 506
AvaI CYCGRG 1 cut(s) 367
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 112
BccI CCATC 3 cut(s) 145, 164, 311
BciT130I CCWGG 2 cut(s) 191, 434
BclI TGATCA 1 cut(s) 352
BfaI CTAG 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 191, 434
BmeT110I CYCGRG 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 2 cut(s) 6, 188
BmrFI CCNGG 2 cut(s) 191, 434
BmsI GCATC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 189, 503
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 283
BseBI CCWGG 2 cut(s) 191, 434
BseDI CCNNGG 2 cut(s) 189, 503
BseGI GGATG 2 cut(s) 133, 322
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 118, 435
BshFI GGCC 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 283
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 4 cut(s) 4, 88, 352, 440
Bsp19I CCATGG 1 cut(s) 503
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 2 cut(s) 117, 436
BspLI GGNNCC 2 cut(s) 6, 188
BspPI GGATC 2 cut(s) 12, 448
BspQI GCTCTTC 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 189, 503
BssMI GATC 4 cut(s) 4, 88, 352, 440
BssT1I CCWWGG 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 191, 434
Bst4CI ACNGT 2 cut(s) 490, 546
Bst6I CTCTTC 2 cut(s) 30, 117
BstDEI CTNAG 4 cut(s) 98, 104, 444, 579
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 2 cut(s) 133, 322
BstKTI GATC 4 cut(s) 7, 91, 355, 443
BstMBI GATC 4 cut(s) 4, 88, 352, 440
BstMWI GCNNNNNNNGC 2 cut(s) 162, 369
BstNI CCWGG 2 cut(s) 191, 434
BstNSI RCATGY 2 cut(s) 397, 517
BstSCI CCNGG 2 cut(s) 189, 432
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 2 cut(s) 4, 440
BstYI RGATCY 2 cut(s) 4, 440
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 2 cut(s) 133, 322
BtsIMutI CAGTG 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 511
CviAII CATG 4 cut(s) 313, 394, 504, 514
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 4 cut(s) 98, 104, 444, 579
DpnI GATC 4 cut(s) 6, 90, 354, 442
DpnII GATC 4 cut(s) 4, 88, 352, 440
Eam1104I CTCTTC 2 cut(s) 30, 117
EarI CTCTTC 2 cut(s) 30, 117
Ecl136II GAGCTC 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 367
EcoICRI GAGCTC 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 189, 432
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 4 cut(s) 316, 397, 507, 517
FaiI YATR 9 cut(s) 60, 168, 242, 300, 314, 357, 395, 505, 515
FatI CATG 4 cut(s) 312, 393, 503, 513
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 403
FokI GGATG 2 cut(s) 120, 329
FriOI GRGCYC 1 cut(s) 112
FspBI CTAG 1 cut(s) 386
HaeIII GGCC 1 cut(s) 187
HapII CCGG 1 cut(s) 245
Hin1II CATG 4 cut(s) 316, 397, 507, 517
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 1 cut(s) 273
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 1 cut(s) 245
HphI GGTGA 2 cut(s) 163, 506
Hpy166II GTNNAC 2 cut(s) 310, 404
Hpy188I TCNGA 5 cut(s) 30, 107, 177, 234, 445
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 2 cut(s) 310, 404
HpyAV CCTTC 1 cut(s) 45
HpyCH4III ACNGT 2 cut(s) 490, 546
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 2 cut(s) 14, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 369
HpyF3I CTNAG 4 cut(s) 98, 104, 444, 579
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 4 cut(s) 316, 397, 507, 517
Ksp22I TGATCA 1 cut(s) 352
KspAI GTTAAC 1 cut(s) 310
Kzo9I GATC 4 cut(s) 4, 88, 352, 440
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 522
LpnPI CCDG 8 cut(s) 132, 176, 203, 258, 290, 402, 419, 446
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 386
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 223, 494
MalI GATC 4 cut(s) 6, 90, 354, 442
MboI GATC 4 cut(s) 4, 88, 352, 440
MboII GAAGA 5 cut(s) 17, 104, 223, 260, 401
MfeI CAATTG 1 cut(s) 478
MflI RGATCY 2 cut(s) 4, 440
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 5 cut(s) 205, 214, 348, 478, 535
MlyI GAGTC 1 cut(s) 282
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 8 cut(s) 70, 113, 154, 253, 262, 282, 417, 501
Mph1103I ATGCAT 1 cut(s) 16
MseI TTAA 2 cut(s) 309, 533
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 2 cut(s) 191, 434
MunI CAATTG 1 cut(s) 478
MvaI CCWGG 2 cut(s) 191, 434
MwoI GCNNNNNNNGC 2 cut(s) 162, 369
NcoI CCATGG 1 cut(s) 503
NdeII GATC 4 cut(s) 4, 88, 352, 440
NlaIII CATG 4 cut(s) 316, 397, 507, 517
NlaIV GGNNCC 2 cut(s) 6, 188
NmuCI GTSAC 2 cut(s) 223, 494
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 2 cut(s) 397, 517
PaeR7I CTCGAG 1 cut(s) 367
PciI ACATGT 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 117
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PscI ACATGT 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 189, 432
PspGI CCWGG 2 cut(s) 189, 432
PspN4I GGNNCC 2 cut(s) 6, 188
PspPI GGNCC 1 cut(s) 186
PspXI VCTCGAGB 1 cut(s) 367
PsuI RGATCY 2 cut(s) 4, 440
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SacI GAGCTC 1 cut(s) 112
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 309, 533
Sau3AI GATC 4 cut(s) 4, 88, 352, 440
Sau96I GGNCC 1 cut(s) 186
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 2 cut(s) 191, 434
SduI GDGCHC 1 cut(s) 112
SfaNI GCATC 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 367
SlaI CTCGAG 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 367
SmoI CTYRAG 1 cut(s) 367
Sse9I AATT 5 cut(s) 205, 214, 348, 478, 535
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 2 cut(s) 189, 432
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 490, 546
TaiI ACGT 1 cut(s) 501
TaqI TCGA 1 cut(s) 368
TasI AATT 5 cut(s) 205, 214, 348, 478, 535
Tru1I TTAA 2 cut(s) 309, 533
Tru9I TTAA 2 cut(s) 309, 533
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 2 cut(s) 223, 494
Tsp45I GTSAC 2 cut(s) 223, 494
TspDTI ATGAA 2 cut(s) 75, 557
TspRI CASTG 1 cut(s) 549
XapI RAATTY 3 cut(s) 205, 214, 535
XceI RCATGY 2 cut(s) 397, 517
XhoI CTCGAG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 403
XspI CTAG 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.