Rh4BG339600

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010081
Physical Location & Seq
Forward (+)
50188426 .. 50189991
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG339600.1

Sequence Viewer

Length: 414 bp
ATGGATCCTAATGCATCAAGCTCTCTTCTGAACCCTTCAAGCTTTCAATCATTTTATTATGAAGCTAAAGAGAGTGAGGGTATTATTGATCTTGGTCTTAGCCTCAGAGCTCTTCAACCTGAAACTTATCATCATCCATCAGCACATTTGGTGAGCTTGGAGGGCTATGATGGTCTGATAGATTGGCCCCAGGCTAACAACTTGAATTTGAAGAATTCAAATGTCACAAGTCCGAGAAATATACCGGAAGATTGTGATGAGGAAGCAGAGGGAGTCCAGAGCAAGGAGAGGTGGGCTTATGTGAAAGTTAACATGGATGGGATTGTTATTGGCAGAAAAGTTTGTGTAATTGATCATAGTGGCTACTCGAGCCTTGCATTTCAACTAGAAGACATGTTTGGTAGGTGCAATTAA

Protein Analysis

137

Amino Acids

15.28

Weight (kDa)

4.65

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 71 - 134 3.5e-10 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15050 AT1G15050 AT2G01200 AT2G01200
fragaria_vesca FvH4_4g26700
malus_domestica MD13G1035200.v1.1
prunus_persica Prupe.1G317000_v2.0.a1
pyrus_communis pycom13g03080
rosa_chinensis RchiOBHm_Chr4g0434391
rosa_laevigata RLG00000006675
rosa_multiflora Rmu_co8521181.1_g000002
rosa_roxburghii Rroxscaffold_5G00375750
rosa_rugosa Rorug02G0288600 Rorug03G0339700 Rorug04G0276300
rosa_samantha Rh4AG331800 Rh4BG339600 Rh4CG354800 Rh4DG040900 Rh4DG334800
rosa_wichuraiana Rw4G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 205, 214
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 7 cut(s) 39, 47, 116, 205, 211, 219, 383
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AluBI AGCT 5 cut(s) 21, 42, 65, 110, 156
AluI AGCT 5 cut(s) 21, 42, 65, 110, 156
Alw21I GWGCWC 1 cut(s) 112
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 367
AoxI GGCC 1 cut(s) 185
ApoI RAATTY 2 cut(s) 205, 214
AspS9I GGNCC 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 367
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 112
BccI CCATC 3 cut(s) 145, 164, 311
BciT130I CCWGG 1 cut(s) 191
BclI TGATCA 1 cut(s) 352
BfaI CTAG 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 2 cut(s) 6, 188
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 189
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 283
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 133, 322
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 118
BshFI GGCC 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 283
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 3 cut(s) 4, 88, 352
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 6, 188
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 117
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 3 cut(s) 4, 88, 352
Bst2UI CCWGG 1 cut(s) 191
Bst6I CTCTTC 2 cut(s) 30, 117
BstDEI CTNAG 2 cut(s) 98, 104
BstF5I GGATG 2 cut(s) 133, 322
BstKTI GATC 3 cut(s) 7, 91, 355
BstMBI GATC 3 cut(s) 4, 88, 352
BstMWI GCNNNNNNNGC 2 cut(s) 162, 369
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 187
BtsCI GGATG 2 cut(s) 133, 322
Cfr13I GGNCC 1 cut(s) 186
CviAII CATG 2 cut(s) 313, 394
DdeI CTNAG 2 cut(s) 98, 104
DpnI GATC 3 cut(s) 6, 90, 354
DpnII GATC 3 cut(s) 4, 88, 352
Eam1104I CTCTTC 2 cut(s) 30, 117
EarI CTCTTC 2 cut(s) 30, 117
Ecl136II GAGCTC 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 367
EcoICRI GAGCTC 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 112
FaeI CATG 2 cut(s) 316, 397
FaiI YATR 7 cut(s) 60, 168, 242, 300, 314, 357, 395
FatI CATG 2 cut(s) 312, 393
FbaI TGATCA 1 cut(s) 352
FokI GGATG 2 cut(s) 120, 329
FriOI GRGCYC 1 cut(s) 112
FspBI CTAG 1 cut(s) 386
HaeIII GGCC 1 cut(s) 187
HapII CCGG 1 cut(s) 245
Hin1II CATG 2 cut(s) 316, 397
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 1 cut(s) 273
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 4 cut(s) 30, 107, 177, 234
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 310
HpyAV CCTTC 1 cut(s) 45
HpyCH4V TGCA 3 cut(s) 14, 377, 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 369
HpyF3I CTNAG 2 cut(s) 98, 104
Hsp92II CATG 2 cut(s) 316, 397
Ksp22I TGATCA 1 cut(s) 352
KspAI GTTAAC 1 cut(s) 310
Kzo9I GATC 3 cut(s) 4, 88, 352
LguI GCTCTTC 1 cut(s) 117
LpnPI CCDG 5 cut(s) 132, 176, 203, 258, 290
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 223
MalI GATC 3 cut(s) 6, 90, 354
MboI GATC 3 cut(s) 4, 88, 352
MboII GAAGA 5 cut(s) 17, 104, 223, 260, 401
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 4 cut(s) 205, 214, 348, 409
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 6 cut(s) 70, 113, 154, 253, 262, 282
Mph1103I ATGCAT 1 cut(s) 16
MseI TTAA 2 cut(s) 309, 412
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 162, 369
NdeII GATC 3 cut(s) 4, 88, 352
NlaIII CATG 2 cut(s) 316, 397
NlaIV GGNNCC 2 cut(s) 6, 188
NmuCI GTSAC 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 1 cut(s) 397
PaeR7I CTCGAG 1 cut(s) 367
PciI ACATGT 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 117
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PscI ACATGT 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 6, 188
PspPI GGNCC 1 cut(s) 186
PspXI VCTCGAGB 1 cut(s) 367
PsuI RGATCY 1 cut(s) 4
SacI GAGCTC 1 cut(s) 112
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 309, 412
Sau3AI GATC 3 cut(s) 4, 88, 352
Sau96I GGNCC 1 cut(s) 186
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 112
SetI ASST 8 cut(s) 23, 44, 67, 112, 121, 158, 293, 407
SfaNI GCATC 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 367
SlaI CTCGAG 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 367
SmoI CTYRAG 1 cut(s) 367
Sse9I AATT 4 cut(s) 205, 214, 348, 409
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 189
TaqI TCGA 1 cut(s) 368
TasI AATT 4 cut(s) 205, 214, 348, 409
Tru1I TTAA 2 cut(s) 309, 412
Tru9I TTAA 2 cut(s) 309, 412
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 75
XapI RAATTY 2 cut(s) 205, 214
XceI RCATGY 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 367
XspI CTAG 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.