Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014499

Physical Location & Seq

Genomic Coordinates Reverse (-)

2737298 .. 2739093

Gene Structure 1796 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1040300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 864 bp

ATGGAGTTTGGCAGGATCCAGATTGTTGTTACCTTGTGCTCGCTGCTCCTTCTTTTTCCTGTGATTCATGGCTGGGGAACTGATGGGCATCTCACAATTTGCAGAATTGCTCAGTCTAGGCTGAGCACGGCGGCGGCGGATGCGGTAAAGCAACTGCTTCCTGACTATGCACAGAATGACTTGGGCAGCCTGTGCATATGGCCAGATCATGTCAAGTTTATTTACCACTGGTCATCTGCCCTTCACTTTATCAACACTCCTGATGTTTGCAATTACCAGTACAATAGGGACTGCAAAGATGAGACTGGAGAGAAGGGGCGTTGTGTTGCGGGGGCAATTAACAATTATACCACCCAGCTTCTCACGTACAGTAGCCATTCTTCTCAACCTAAATATAACCTCACGCAGGCGCTTCTATTTCTTTCCCATTTCATAGGAGACATCCATCAGCCTCTGCATGTAGGTTTTGGTCCGGACAAGGGAGGCAACACAATTGATGTTCACTGGTACACAACGAAGCAAAATCTTCATCATATATGGGATTCCAACATAATTGAGACAGCGGAAGAGAGATTCTACAATTCCAACGTCGAAGAACTGATTGACGCGATTAAACAGAACATTACGACTGAATGGTCAGATCAAGTTAAGGGTTGGGAGACCTGCAGCCTAAACAAGACAGCGTGCCCCGACGTATACGCATCTGAAGGCATCAAAGCAGCTTGTGATTGGGCATACAAGGGTGCAAATGAAGGTTCAGTGCTAGAAGATGAATACTTCCTCTCCCGGCTACCAATAGTCCATAGGCGGTTAGCTCAAGGTGGCGTCCGTCTAGCAGCCACCCTCAACCGCATCTTCGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0006139 GO:0006259 GO:0006308 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0016788 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0046483 GO:0046700 GO:0071704 GO:0090304 GO:0090305 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

S1-P1_nuclease

Protein Analysis

288

Amino Acids

32.32

Weight (kDa)

5.87

Isoelectric Point (pI)

33.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1-P1_nuclease	PF02265	25 - 286	2.8e-84	S1/P1 Nuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68290
fragaria_vesca	FvH4_4g27190
malus_domestica	MD13G1040300.v1.1 MD16G1041300.v1.1
prunus_persica	Prupe.1G312700_v2.0.a1
pyrus_communis	pycom16g03580
rosa_chinensis	RchiOBHm_Chr4g0434991
rosa_laevigata	RLG00000006631
rosa_multiflora	Rmu_sc0000400.1_g000035 Rmu_sc0023421.1_g000001
rosa_roxburghii	Rroxscaffold_5G00376160
rosa_rugosa	Rorug04G0281500 Rorug04G0281500
rosa_samantha	Rh4AG336900 Rh4BG344800 Rh4DG339300
rosa_wichuraiana	Rw4G029370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	634
Acc36I	ACCTGC	1 cut(s)	671
AccI	GTMKAC	1 cut(s)	696
AccII	CGCG	1 cut(s)	608
AccIII	TCCGGA	1 cut(s)	472
AciI	CCGC	8 cut(s)	131, 134, 137, 143, 329, 563, 808, 850
AclWI	GGATC	2 cut(s)	10, 23
AcoI	YGGCCR	1 cut(s)	200
AcuI	CTGAAG	1 cut(s)	726
AcyI	GRCGYC	1 cut(s)	825
AfaI	GTAC	3 cut(s)	281, 368, 509
AfiI	CCNNNNNNNGG	2 cut(s)	406, 479
AluBI	AGCT	3 cut(s)	358, 722, 815
AluI	AGCT	3 cut(s)	358, 722, 815
Alw21I	GWGCWC	2 cut(s)	41, 128
Alw26I	GTCTC	4 cut(s)	296, 432, 551, 653
AlwI	GGATC	2 cut(s)	10, 23
AlwNI	CAGNNNCTG	1 cut(s)	454
Aor13HI	TCCGGA	1 cut(s)	472
AoxI	GGCC	1 cut(s)	200
ApeKI	GCWGC	5 cut(s)	43, 186, 666, 719, 836
AspLEI	GCGC	1 cut(s)	412
AspS9I	GGNCC	1 cut(s)	470
AsuC2I	CCSGG	1 cut(s)	787
AvaII	GGWCC	1 cut(s)	470
BaeGI	GKGCMC	1 cut(s)	689
BalI	TGGCCA	1 cut(s)	202
BamHI	GGATCC	1 cut(s)	15
BarI	GAAGNNNNNNTAC	2 cut(s)	364, 396
Bbv12I	GWGCWC	2 cut(s)	41, 128
BbvI	GCAGC	5 cut(s)	30, 198, 678, 731, 848
BccI	CCATC	2 cut(s)	77, 453
BceAI	ACGGC	1 cut(s)	144
BcnI	CCSGG	1 cut(s)	787
BcoDI	GTCTC	4 cut(s)	296, 432, 551, 653
BfaI	CTAG	3 cut(s)	117, 764, 833
BfmI	CTRYAG	1 cut(s)	664
BfoI	RGCGCY	1 cut(s)	413
BfuAI	ACCTGC	1 cut(s)	671
BisI	GCNGC	7 cut(s)	44, 132, 135, 187, 667, 720, 837
BlpI	GCTNAGC	1 cut(s)	122
BlsI	GCNGC	7 cut(s)	45, 133, 136, 188, 668, 721, 838
Bme1390I	CCNGG	1 cut(s)	787
Bme18I	GGWCC	1 cut(s)	470
BmgT120I	GGNCC	1 cut(s)	470
BmiI	GGNNCC	1 cut(s)	17
BmrFI	CCNGG	1 cut(s)	787
BmsI	GCATC	4 cut(s)	97, 130, 710, 720
BpmI	CTGGAG	1 cut(s)	327
Bpu1102I	GCTNAGC	1 cut(s)	122
BpuEI	CTTGAG	1 cut(s)	801
BpuMI	CCSGG	1 cut(s)	787
BsaAI	YACGTR	1 cut(s)	366
BsaBI	GATNNNNATC	1 cut(s)	87
BsaHI	GRCGYC	1 cut(s)	825
BsaI	GGTCTC	1 cut(s)	653
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	2 cut(s)	767, 797
Bsc4I	CCNNNNNNNGG	2 cut(s)	406, 479
Bse1I	ACTGG	4 cut(s)	233, 277, 310, 509
Bse8I	GATNNNNATC	1 cut(s)	87
BseAI	TCCGGA	1 cut(s)	472
BseGI	GGATG	2 cut(s)	145, 441
BseJI	GATNNNNATC	1 cut(s)	87
BseLI	CCNNNNNNNGG	2 cut(s)	406, 479
BseMII	CTCAG	2 cut(s)	113, 125
BseNI	ACTGG	4 cut(s)	233, 277, 310, 509
BseSI	GKGCMC	1 cut(s)	689
BseXI	GCAGC	5 cut(s)	30, 198, 678, 731, 848
BseYI	CCCAGC	2 cut(s)	72, 354
Bsh1236I	CGCG	1 cut(s)	608
BshFI	GGCC	1 cut(s)	202
BsiHKAI	GWGCWC	2 cut(s)	41, 128
BsiSI	CCGG	2 cut(s)	473, 787
BslFI	GGGAC	1 cut(s)	302
BslI	CCNNNNNNNGG	2 cut(s)	406, 479
BsmAI	GTCTC	4 cut(s)	296, 432, 551, 653
BsmFI	GGGAC	1 cut(s)	302
BsnI	GGCC	1 cut(s)	202
Bso31I	GGTCTC	1 cut(s)	653
Bsp1286I	GDGCHC	3 cut(s)	41, 128, 689
Bsp13I	TCCGGA	1 cut(s)	472
Bsp143I	GATC	3 cut(s)	15, 205, 640
Bsp1720I	GCTNAGC	1 cut(s)	122
BspACI	CCGC	8 cut(s)	131, 134, 137, 143, 329, 563, 808, 850
BspANI	GGCC	1 cut(s)	202
BspCNI	CTCAG	2 cut(s)	114, 124
BspEI	TCCGGA	1 cut(s)	472
BspFNI	CGCG	1 cut(s)	608
BspLI	GGNNCC	1 cut(s)	17
BspMAI	CTGCAG	1 cut(s)	668
BspMI	ACCTGC	1 cut(s)	671
BspPI	GGATC	2 cut(s)	10, 23
BspTNI	GGTCTC	1 cut(s)	653
BsrI	ACTGG	4 cut(s)	233, 277, 310, 509
BssMI	GATC	3 cut(s)	15, 205, 640
BssNAI	GTATAC	1 cut(s)	697
BssNI	GRCGYC	1 cut(s)	825
Bst1107I	GTATAC	1 cut(s)	697
Bst4CI	ACNGT	1 cut(s)	371
Bst6I	CTCTTC	1 cut(s)	561
BstACI	GRCGYC	1 cut(s)	825
BstAPI	GCANNNNNTGC	1 cut(s)	192
BstBAI	YACGTR	1 cut(s)	366
BstC8I	GCNNGC	3 cut(s)	41, 408, 685
BstDEI	CTNAG	2 cut(s)	111, 122
BstENI	CCTNNNNNAGG	1 cut(s)	404
BstF5I	GGATG	2 cut(s)	145, 441
BstFNI	CGCG	1 cut(s)	608
BstH2I	RGCGCY	1 cut(s)	413
BstHHI	GCGC	1 cut(s)	412
BstKTI	GATC	3 cut(s)	18, 208, 643
BstMAI	GTCTC	4 cut(s)	296, 432, 551, 653
BstMBI	GATC	3 cut(s)	15, 205, 640
BstMWI	GCNNNNNNNGC	2 cut(s)	140, 192
BstNSI	RCATGY	1 cut(s)	461
BstSCI	CCNGG	1 cut(s)	785
BstSFI	CTRYAG	1 cut(s)	664
BstSLI	GKGCMC	1 cut(s)	689
BstUI	CGCG	1 cut(s)	608
BstV1I	GCAGC	5 cut(s)	30, 198, 678, 731, 848
BstX2I	RGATCY	1 cut(s)	15
BstYI	RGATCY	1 cut(s)	15
BstZ17I	GTATAC	1 cut(s)	697
BsuRI	GGCC	1 cut(s)	202
BtsCI	GGATG	2 cut(s)	145, 441
BtsIMutI	CAGTG	3 cut(s)	226, 502, 765
BveI	ACCTGC	1 cut(s)	671
Cac8I	GCNNGC	3 cut(s)	41, 408, 685
CaiI	CAGNNNCTG	1 cut(s)	454
CfoI	GCGC	1 cut(s)	412
Cfr13I	GGNCC	1 cut(s)	470
CseI	GACGC	2 cut(s)	614, 814
Csp6I	GTAC	3 cut(s)	280, 367, 508
CviAII	CATG	3 cut(s)	68, 209, 458
CviQI	GTAC	3 cut(s)	280, 367, 508
DdeI	CTNAG	2 cut(s)	111, 122
DpnI	GATC	3 cut(s)	17, 207, 642
DpnII	GATC	3 cut(s)	15, 205, 640
DrdI	GACNNNNNNGTC	1 cut(s)	634
DseDI	GACNNNNNNGTC	1 cut(s)	634
EaeI	YGGCCR	1 cut(s)	200
Eam1104I	CTCTTC	1 cut(s)	561
EarI	CTCTTC	1 cut(s)	561
EciI	GGCGGA	1 cut(s)	152
Eco31I	GGTCTC	1 cut(s)	653
Eco47I	GGWCC	1 cut(s)	470
Eco57I	CTGAAG	1 cut(s)	726
EcoNI	CCTNNNNNAGG	1 cut(s)	404
FaeI	CATG	3 cut(s)	71, 212, 461
FaqI	GGGAC	1 cut(s)	302
FatI	CATG	3 cut(s)	67, 208, 457
FauI	CCCGC	1 cut(s)	322
FauNDI	CATATG	1 cut(s)	197
FblI	GTMKAC	1 cut(s)	696
Fnu4HI	GCNGC	7 cut(s)	44, 132, 135, 187, 667, 720, 837
FokI	GGATG	2 cut(s)	152, 428
Fsp4HI	GCNGC	7 cut(s)	44, 132, 135, 187, 667, 720, 837
FspBI	CTAG	3 cut(s)	117, 764, 833
GlaI	GCGC	1 cut(s)	411
GluI	GCNGC	7 cut(s)	44, 132, 135, 187, 667, 720, 837
GsaI	CCCAGC	2 cut(s)	76, 358
GsuI	CTGGAG	1 cut(s)	327
HaeII	RGCGCY	1 cut(s)	413
HaeIII	GGCC	1 cut(s)	202
HapII	CCGG	2 cut(s)	473, 787
HgaI	GACGC	2 cut(s)	614, 814
HhaI	GCGC	1 cut(s)	412
Hin1I	GRCGYC	1 cut(s)	825
Hin1II	CATG	3 cut(s)	71, 212, 461
Hin6I	GCGC	1 cut(s)	410
HinP1I	GCGC	1 cut(s)	410
HinfI	GANTC	3 cut(s)	64, 542, 573
HpaII	CCGG	2 cut(s)	473, 787
Hpy166II	GTNNAC	3 cut(s)	502, 510, 697
Hpy188I	TCNGA	2 cut(s)	640, 706
Hpy188III	TCNNGA	4 cut(s)	19, 161, 260, 473
Hpy8I	GTNNAC	3 cut(s)	502, 510, 697
Hpy99I	CGWCG	2 cut(s)	593, 695
HpyAV	CCTTC	5 cut(s)	59, 251, 307, 701, 746
HpyCH4III	ACNGT	1 cut(s)	371
HpyCH4IV	ACGT	3 cut(s)	365, 588, 693
HpyCH4V	TGCA	8 cut(s)	102, 170, 195, 270, 294, 457, 666, 746
HpyF10VI	GCNNNNNNNGC	2 cut(s)	140, 192
HpyF3I	CTNAG	2 cut(s)	111, 122
HpySE526I	ACGT	3 cut(s)	365, 588, 693
Hsp92I	GRCGYC	1 cut(s)	825
Hsp92II	CATG	3 cut(s)	71, 212, 461
HspAI	GCGC	1 cut(s)	410
Kpn2I	TCCGGA	1 cut(s)	472
Kzo9I	GATC	3 cut(s)	15, 205, 640
LmnI	GCTCC	1 cut(s)	51
Lsp1109I	GCAGC	5 cut(s)	30, 198, 678, 731, 848
LweI	GCATC	4 cut(s)	97, 130, 710, 720
MaeI	CTAG	3 cut(s)	117, 764, 833
MaeII	ACGT	3 cut(s)	365, 588, 693
MaeIII	GTNAC	1 cut(s)	28
MalI	GATC	3 cut(s)	17, 207, 642
MboI	GATC	3 cut(s)	15, 205, 640
MboII	GAAGA	6 cut(s)	372, 518, 578, 605, 779, 847
MfeI	CAATTG	1 cut(s)	492
MflI	RGATCY	1 cut(s)	15
MhlI	GDGCHC	3 cut(s)	41, 128, 689
MlsI	TGGCCA	1 cut(s)	202
MluCI	AATT	8 cut(s)	96, 105, 271, 336, 343, 492, 552, 580
MluNI	TGGCCA	1 cut(s)	202
MmeI	TCCRAC	2 cut(s)	570, 609
MnlI	CCTC	5 cut(s)	410, 462, 476, 791, 854
Mox20I	TGGCCA	1 cut(s)	202
MroI	TCCGGA	1 cut(s)	472
MscI	TGGCCA	1 cut(s)	202
MseI	TTAA	3 cut(s)	339, 612, 648
Msp20I	TGGCCA	1 cut(s)	202
MspA1I	CMGCKG	1 cut(s)	563
MspI	CCGG	2 cut(s)	473, 787
MspR9I	CCNGG	1 cut(s)	787
MunI	CAATTG	1 cut(s)	492
MvnI	CGCG	1 cut(s)	608
MwoI	GCNNNNNNNGC	2 cut(s)	140, 192
NciI	CCSGG	1 cut(s)	787
NdeI	CATATG	1 cut(s)	197
NdeII	GATC	3 cut(s)	15, 205, 640
NlaIII	CATG	3 cut(s)	71, 212, 461
NlaIV	GGNNCC	1 cut(s)	17
NspI	RCATGY	1 cut(s)	461
PfeI	GAWTC	3 cut(s)	64, 542, 573
PkrI	GCNGC	7 cut(s)	45, 133, 136, 188, 668, 721, 838
Ppu21I	YACGTR	1 cut(s)	366
PspFI	CCCAGC	2 cut(s)	72, 354
PspN4I	GGNNCC	1 cut(s)	17
PspPI	GGNCC	1 cut(s)	470
PstI	CTGCAG	1 cut(s)	668
PstNI	CAGNNNCTG	1 cut(s)	454
PsuI	RGATCY	1 cut(s)	15
RsaI	GTAC	3 cut(s)	281, 368, 509
RsaNI	GTAC	3 cut(s)	280, 367, 508
SaqAI	TTAA	3 cut(s)	339, 612, 648
SatI	GCNGC	7 cut(s)	44, 132, 135, 187, 667, 720, 837
Sau3AI	GATC	3 cut(s)	15, 205, 640
Sau96I	GGNCC	1 cut(s)	470
ScrFI	CCNGG	1 cut(s)	787
SduI	GDGCHC	3 cut(s)	41, 128, 689
SfaNI	GCATC	4 cut(s)	97, 130, 710, 720
SfcI	CTRYAG	1 cut(s)	664
SinI	GGWCC	1 cut(s)	470
SmlI	CTYRAG	1 cut(s)	816
SmoI	CTYRAG	1 cut(s)	816
Sse9I	AATT	8 cut(s)	96, 105, 271, 336, 343, 492, 552, 580
SsiI	CCGC	8 cut(s)	131, 134, 137, 143, 329, 563, 808, 850
SspMI	CTAG	3 cut(s)	117, 764, 833
StyD4I	CCNGG	1 cut(s)	785
TaaI	ACNGT	1 cut(s)	371
TaiI	ACGT	3 cut(s)	368, 591, 696
TaqI	TCGA	1 cut(s)	591
TasI	AATT	8 cut(s)	96, 105, 271, 336, 343, 492, 552, 580
TatI	WGTACW	1 cut(s)	279
TauI	GCSGC	2 cut(s)	134, 137
TfiI	GAWTC	3 cut(s)	64, 542, 573
Tru1I	TTAA	3 cut(s)	339, 612, 648
Tru9I	TTAA	3 cut(s)	339, 612, 648
TscAI	CASTG	3 cut(s)	233, 509, 765
TseI	GCWGC	5 cut(s)	43, 186, 666, 719, 836
TspDTI	ATGAA	5 cut(s)	56, 421, 518, 765, 786
TspGWI	ACGGA	1 cut(s)	818
TspRI	CASTG	3 cut(s)	233, 509, 765
VpaK11BI	GGWCC	1 cut(s)	470
XagI	CCTNNNNNAGG	1 cut(s)	404
XceI	RCATGY	1 cut(s)	461
XmiI	GTMKAC	1 cut(s)	696
XspI	CTAG	3 cut(s)	117, 764, 833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.