MD16G1041300.v1.1

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014499
Physical Location & Seq
Reverse (-)
2911391 .. 2912711
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1041300.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGAGTGTGGCAGGATCCAGATTGTTATTGCCTTGTGCTTGCTGCTCCTTTTTCCTGTGATTCATGGCTGGGGCAATGATGGGCATGTCACAATCTGCAGAATTGCTCAGGTTCTATCCAACGTCATATTTTTCTCATACCTCGGCTTAACCTTGGCTAGATCTAGGCTGAGCAAGGCGGCGGCGGATGCGGTGAAGCAACTGCTTCCAGACTATGCAGAGAATGACTGGAGCAGCCTGTGCTTATGGGCAGATCGTGTCAAGTTTATTTTTCCCTGGTCATCTGCCCTTCACTACATTAACACTCCTGAAGTTTGCAATTACCAGTACAGTAGGGACTGCAAAGATGAGGATGGAGAGAAGGGGCGTTGTGTTGCAGGGGCAATTAAGAATTACACCACTCAGCTTCTGACCTACAATAGCCAGTTGTCTAAACCTAAATATAACCTCACGCAGGCGTTTCTATTTCTTTCCCATTTCATAGGAGACATCCATCAGCCTCTGCATGTAGGTTTTGCTTCGGACAAGGGAGGCAACACAATTGACGTTCACTGGTATACGAGGAATCAAAATCTTCATCATATTTGGGATGACAACATAATTGAGACGGCGGAAGAGAGGTTTTACGATTCCAATGTCGAAGAACTAATTGACGCAATTAAACAGAACATTACGGTATCTTAG

Protein Analysis

228

Amino Acids

25.89

Weight (kDa)

5.81

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 52 - 225 9.9e-52 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68290
fragaria_vesca FvH4_4g27190
malus_domestica MD13G1040300.v1.1 MD16G1041300.v1.1
prunus_persica Prupe.1G312700_v2.0.a1
pyrus_communis pycom16g03580
rosa_chinensis RchiOBHm_Chr4g0434991
rosa_laevigata RLG00000006631
rosa_multiflora Rmu_sc0000400.1_g000035 Rmu_sc0023421.1_g000001
rosa_roxburghii Rroxscaffold_5G00376160
rosa_rugosa Rorug04G0281500 Rorug04G0281500
rosa_samantha Rh4AG336900 Rh4BG344800 Rh4DG339300
rosa_wichuraiana Rw4G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 557
AciI CCGC 5 cut(s) 179, 182, 185, 191, 611
AclWI GGATC 2 cut(s) 10, 23
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 454
AjnI CCWGG 1 cut(s) 275
AluBI AGCT 1 cut(s) 406
AluI AGCT 1 cut(s) 406
Alw26I GTCTC 2 cut(s) 480, 599
AlwI GGATC 2 cut(s) 10, 23
AlwNI CAGNNNCTG 2 cut(s) 409, 502
ApeKI GCWGC 2 cut(s) 43, 234
AsuHPI GGTGA 1 cut(s) 205
BamHI GGATCC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 30, 246
BccI CCATC 3 cut(s) 74, 347, 501
BceAI ACGGC 1 cut(s) 624
BciT130I CCWGG 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 480, 599
BfaI CTAG 2 cut(s) 159, 165
BfmI CTRYAG 1 cut(s) 97
BglII AGATCT 1 cut(s) 161
BisI GCNGC 4 cut(s) 44, 180, 183, 235
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 4 cut(s) 45, 181, 184, 236
Bme1390I CCNGG 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 250
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 142, 153, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse1I ACTGG 4 cut(s) 233, 325, 424, 557
Bse3DI GCAATG 1 cut(s) 82
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 3 cut(s) 142, 153, 275
BseGI GGATG 4 cut(s) 193, 358, 489, 595
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 3 cut(s) 122, 161, 416
BseNI ACTGG 4 cut(s) 233, 325, 424, 557
BseXI GCAGC 2 cut(s) 30, 246
BseYI CCCAGC 1 cut(s) 69
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 2 cut(s) 480, 599
BsmBI CGTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 350
Bsp143I GATC 3 cut(s) 15, 161, 253
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 5 cut(s) 179, 182, 185, 191, 611
BspCNI CTCAG 3 cut(s) 121, 162, 415
BspLI GGNNCC 1 cut(s) 17
BspMAI CTGCAG 1 cut(s) 101
BspPI GGATC 2 cut(s) 10, 23
BsrDI GCAATG 1 cut(s) 82
BsrI ACTGG 4 cut(s) 233, 325, 424, 557
BssECI CCNNGG 3 cut(s) 142, 153, 275
BssMI GATC 3 cut(s) 15, 161, 253
BssNAI GTATAC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 153
Bst1107I GTATAC 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 2 cut(s) 332, 676
Bst6I CTCTTC 1 cut(s) 609
BstAPI GCANNNNNTGC 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 41, 456
BstDEI CTNAG 4 cut(s) 108, 170, 402, 681
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 4 cut(s) 193, 358, 489, 595
BstKTI GATC 3 cut(s) 18, 164, 256
BstMAI GTCTC 2 cut(s) 480, 599
BstMBI GATC 3 cut(s) 15, 161, 253
BstMWI GCNNNNNNNGC 2 cut(s) 188, 240
BstNI CCWGG 1 cut(s) 277
BstNSI RCATGY 2 cut(s) 89, 509
BstSCI CCNGG 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 97
BstV1I GCAGC 2 cut(s) 30, 246
BstX2I RGATCY 2 cut(s) 15, 161
BstYI RGATCY 2 cut(s) 15, 161
BstZ17I GTATAC 1 cut(s) 558
BtsCI GGATG 4 cut(s) 193, 358, 489, 595
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 2 cut(s) 41, 456
CaiI CAGNNNCTG 2 cut(s) 409, 502
CseI GACGC 1 cut(s) 662
Csp6I GTAC 1 cut(s) 328
CviAII CATG 3 cut(s) 65, 86, 506
CviJI RGCY 8 cut(s) 69, 147, 158, 169, 237, 406, 423, 499
CviKI_1 RGCY 8 cut(s) 69, 147, 158, 169, 237, 406, 423, 499
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 4 cut(s) 108, 170, 402, 681
DpnI GATC 3 cut(s) 17, 163, 255
DpnII GATC 3 cut(s) 15, 161, 253
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
EciI GGCGGA 2 cut(s) 200, 626
Eco130I CCWWGG 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 599
FaeI CATG 3 cut(s) 68, 89, 509
FaqI GGGAC 1 cut(s) 350
FatI CATG 3 cut(s) 64, 85, 505
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 4 cut(s) 44, 180, 183, 235
FokI GGATG 4 cut(s) 200, 365, 476, 602
Fsp4HI GCNGC 4 cut(s) 44, 180, 183, 235
FspBI CTAG 2 cut(s) 159, 165
GluI GCNGC 4 cut(s) 44, 180, 183, 235
GsaI CCCAGC 1 cut(s) 73
GsuI CTGGAG 1 cut(s) 250
HgaI GACGC 1 cut(s) 662
Hin1II CATG 3 cut(s) 68, 89, 509
HinfI GANTC 3 cut(s) 61, 565, 629
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 550, 558
Hpy188I TCNGA 2 cut(s) 411, 523
Hpy188III TCNNGA 3 cut(s) 19, 209, 308
Hpy8I GTNNAC 2 cut(s) 550, 558
HpyAV CCTTC 2 cut(s) 299, 355
HpyCH4III ACNGT 2 cut(s) 332, 676
HpyCH4IV ACGT 2 cut(s) 123, 546
HpyCH4V TGCA 6 cut(s) 99, 218, 318, 342, 377, 505
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 240
HpyF3I CTNAG 4 cut(s) 108, 170, 402, 681
HpySE526I ACGT 2 cut(s) 123, 546
Hsp92II CATG 3 cut(s) 68, 89, 509
Kzo9I GATC 3 cut(s) 15, 161, 253
LmnI GCTCC 2 cut(s) 51, 231
Lsp1109I GCAGC 2 cut(s) 30, 246
LweI GCATC 1 cut(s) 178
MaeI CTAG 2 cut(s) 159, 165
MaeII ACGT 2 cut(s) 123, 546
MaeIII GTNAC 1 cut(s) 88
MalI GATC 3 cut(s) 17, 163, 255
MboI GATC 3 cut(s) 15, 161, 253
MboII GAAGA 3 cut(s) 566, 626, 653
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 2 cut(s) 15, 161
MluCI AATT 8 cut(s) 102, 319, 384, 391, 540, 600, 648, 657
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 7 cut(s) 152, 343, 458, 510, 524, 555, 612
MseI TTAA 4 cut(s) 149, 300, 387, 660
MspR9I CCNGG 1 cut(s) 277
MunI CAATTG 1 cut(s) 540
MvaI CCWGG 1 cut(s) 277
MwoI GCNNNNNNNGC 2 cut(s) 188, 240
NdeII GATC 3 cut(s) 15, 161, 253
NlaIII CATG 3 cut(s) 68, 89, 509
NlaIV GGNNCC 1 cut(s) 17
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 2 cut(s) 89, 509
PfeI GAWTC 3 cut(s) 61, 565, 629
PkrI GCNGC 4 cut(s) 45, 181, 184, 236
Psp6I CCWGG 1 cut(s) 275
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 17
PstI CTGCAG 1 cut(s) 101
PstNI CAGNNNCTG 2 cut(s) 409, 502
PsuI RGATCY 2 cut(s) 15, 161
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 4 cut(s) 149, 300, 387, 660
SatI GCNGC 4 cut(s) 44, 180, 183, 235
Sau3AI GATC 3 cut(s) 15, 161, 253
ScrFI CCNGG 1 cut(s) 277
SfaNI GCATC 1 cut(s) 178
SfcI CTRYAG 1 cut(s) 97
Sse9I AATT 8 cut(s) 102, 319, 384, 391, 540, 600, 648, 657
SsiI CCGC 5 cut(s) 179, 182, 185, 191, 611
SspMI CTAG 2 cut(s) 159, 165
StyD4I CCNGG 1 cut(s) 275
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 332, 676
TaiI ACGT 2 cut(s) 126, 549
TaqI TCGA 1 cut(s) 639
TasI AATT 8 cut(s) 102, 319, 384, 391, 540, 600, 648, 657
TatI WGTACW 1 cut(s) 327
TauI GCSGC 2 cut(s) 182, 185
TfiI GAWTC 3 cut(s) 61, 565, 629
Tru1I TTAA 4 cut(s) 149, 300, 387, 660
Tru9I TTAA 4 cut(s) 149, 300, 387, 660
TscAI CASTG 1 cut(s) 557
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 43, 234
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 3 cut(s) 53, 469, 566
TspRI CASTG 1 cut(s) 557
XagI CCTNNNNNAGG 1 cut(s) 452
XceI RCATGY 2 cut(s) 89, 509
XmiI GTMKAC 1 cut(s) 557
XspI CTAG 2 cut(s) 159, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.